CopoB.01659.a

Spherule outer wall glycoprotein

CENTER ID:	CopoB.01659.a
ORGANISM:	Coccidioides posadasii Silveira
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CopoB.01659.a.D21.GE082212a	mature protein		61	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CPSG_06466
OrthoMCL:	OG5_223177
UniProt:	Q8NK60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIFRIIIIS AIAACPFAIG YEYPPHDCQS YGDDYGNCKG EKPSATPSHY DEYGYKMRKR GATSHKEHSY CDTYGCDGPM EPKTPKPTDC YGDCEDGYDY SPPPPPKKYG DCDDYDGYCD GPSKTSMKPE PPKPTDCYGD CEDGYDYSPP PPPKKYGDCD DYDGYCDGPS KTSMKPEPPK PTDCYGDCED GYDYSPPPPP KKYGDCDDYD GYCDGPSKTS IKPEPPKPTD CYGDCEDGYD YSPPPPPKKY GDCDYDDGYC DGSPPPKETK NYGYHYARAH DKAASSGSTD RPASTPVHTP TQFEGAAGIL QPRGISFVAL AIAAAFYV

NT Sequence

atgaaaatct ttcgcataat catcatctca gccatagcag cttgtccctt tgccatcggc tacgagtatc cgccacatga ttgccaatct tacggtgatg attatggaaa ttgtaaagga gagaagcctt ctgccacgcc cagtcattat gatgaatatg gatataaaat gaggaaacga ggtgctacga gccacaaaga gcacagttat tgcgatacct atggttgtga cggcccaatg gagccaaaaa cccctaagcc aacagattgt tatggtgact gcgaggatgg gtatgattac tcccctccac ctcctcccaa gaagtatggt gactgtgatg attatgatgg gtattgtgat ggtccctcaa aaacctcaat gaagccagaa ccccctaagc caacagattg ttatggtgac tgcgaggatg ggtatgatta ctcccctcca cctcctccca agaagtatgg tgactgtgat gattatgacg ggtattgtga tggtccctca aaaacctcaa tgaagccaga accccctaag ccaacagatt gttatggtga ctgtgaggat gggtatgatt actcccctcc accacctccc aagaagtatg gtgactgtga tgattatgat gggtattgtg atggcccctc aaaaacttca ataaagccag aaccccctaa gccaacagat tgttatggtg actgtgagga tgggtatgat tactcccctc caccacctcc caagaagtat ggcgactgtg attatgatga tgggtattgc gacggttccc ctccacctaa ggaaacaaag aattatggct accattatgc cagagcccat gacaaggcag cttcctctgg cagcacagac cggccagctt ctacaccagt tcatacgcca actcagtttg agggggctgc aggaatcttg cagccgcggg gtatctcttt cgtcgccctt gctatcgcag ctgcattcta tgtttga

Details for CopoB.01659.a.D21.GE082212a

HARVESTED ON:	2/8/2010
SEQUENCED ON:	9/18/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	74
PERCENT COVERAGE:	85

Validated AA Sequence

MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG GAXSHKEXSY XXTXGCDGXX EPXTXXXXDC YGDCEXGYDY SXPPPXXXXG DXXXXXXYXX XXXXTSXKPX XXXXXXCYGX XEDGYDYXXX PXPXXXXXXX DXXXYCXXXS XXSMXXEPXX XXDXXXXXED GYDYSPPPPP KKYGDCDDYD GYCDGPSKTS IKPEPPKPTD XYGDCEDGYD YSPPPPPKKY GDCXXDDGXX DGSPPPKETK NYGXHYARAH DKAASSGSTD RPASTPVHTP TQFEGAXGIL QPRGXSXVAL X

Validated NT Sequence

ggcgaagtaa aaccggaagt gaagccggag acccacatca acctgaaggt tagcgacggt agcagcgaaa tcttcttcaa gattaagaag accaccccgc tgcgtcgcct gatggaagca ttcgctaaac gccagggcaa ggaaatggat tccctgcgct ttctgtacga cggtatccgt attcaggcag accagactcc ggaagatctg gacatggaag ataacgacat tatcgaagca caccgtgaac aaatcggtgg aggcgctncn tcncataaag agnantctta ttgnnatacn tncggntgtg acggnnnnnn ggaaccnaan actncnaann nnantgactg ctatggtgac tgtgaanatg gttacgatta ttctnctccn cctccnnnna nnnnnnnngg tgactgnnnn nacnnnnann gctatngnga nngnnnnnnn nnnacctccn tgaaaccaga nnnnnnnnnn nnnnctgant gctatggnna tngtgaggat ggttatgact atnnnncacn tccgnntccg aanaantann gtgantgnna cgatnncnac ngttactgcn acngtncgtc cnanantagc atgaannctg aaccgncnnn nnnnancgat tnnnnnnnnn nnngcgaaga tggttacgac tattctcctc cgcctccgcc gaagaagtac ggtgattgcg acgattacga cggttattgt gacggtccta gcaagacctc cattaaaccg gagcctccaa agccaaccga ctgntacggc gactgtgaag acggttacga ttactctcca ccgccacctc cgaagaaata tggngactgc gantncgatg atggctantg ngatggtagc ccgccaccga aggaaaccaa gaattacggt tnccattacg cccgtgccca tgacaaagca gcttcctctg gcagcaccga tcgtcctgct agcactcctg ttcacactcc gacccagttt gaaggtgcnn cnggtatcct gcagcctcgt ggcatntcnt tngtcgccct gnctat

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGGATS HKEHSYCDTY GCDGPMEPKT PKPTDCYGDC EDGYDYSPPP PPKKYGDCDD YDGYCDGPSK TSMKPEPPKP TDCYGDCEDG YDYSPPPPPK KYGDCDDYDG YCDGPSKTSM KPEPPKPTDC YGDCEDGYDY SPPPPPKKYG DCDDYDGYCD GPSKTSIKPE PPKPTDCYGD CEDGYDYSPP PPPKKYGDCD YDDGYCDGSP PPKETKNYGY HYARAHDKAA SSGSTDRPAS TPVHTPTQFE GAAGILQPRG ISFVALAIAA AFYV

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagg cgctacgtcc cataaagagc actcttattg cgatacgtac ggctgtgacg gcccgatgga accgaagact ccgaagccga ctgactgcta tggtgactgt gaagatggtt acgattattc tcctccgcct ccgccaaaga aatatggtga ctgtgacgac tacgacggct attgtgacgg tccgtccaaa acctccatga aaccagaacc accgaaacca actgactgct atggcgattg tgaggatggt tatgactata gcccacctcc gcctccgaag aagtacggtg attgcgacga ttacgacggt tactgcgacg gtccgtccaa gactagcatg aagcctgaac cgcctaaacc gaccgattgc tatggcgact gcgaagatgg ttacgactat tctcctccgc ctccgccgaa gaagtacggt gattgcgacg attacgacgg ttattgtgac ggtcctagca agacctccat taaaccggag cctccaaagc caaccgactg ctacggcgac tgtgaagacg gttacgatta ctctccaccg ccacctccga agaaatatgg cgactgcgac tacgatgatg gctactgcga tggtagcccg ccaccgaagg aaaccaagaa ttacggttac cattacgccc gtgcccatga caaagcagct tcctctggca gcaccgatcg tcctgctagc actcctgttc acactccgac ccagtttgaa ggtgctgccg gtatcctgca gcctcgtggc atctctttcg tcgccctggc tattgccgca gcattctatg tttaaaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat