SSGCID: Target Page

CoimA.01544.a

Putative uncharacterized protein

CENTER ID:	CoimA.01544.a
ORGANISM:	Coccidioides immitis RS
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.01544.a.A1.GE30648	Full length( CoimA.01544.a )		1	215

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CIMG_12809
UniProt:	Q1E8U5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDDVKKGILL QLFGGTNKTF EKGGSPRYRG DINVLLCGDP STSKSQILQY VHKIAPRGIY TSGKGSSAVG LTAYVTRDPE SRQLVLESGA LVLSDGGVCC IDEFDKMNEA TRSVLHEVME QQTVSIAKAG IITTLNARTS ILASANPIGS KYNPNLPVPQ NIDLPPTLLS RFDLVYLVLD RIDEQNDRRL AKHLVGMYLE DTPENASTEE ILVCL

NT Sequence

ATGGACGACG TGAAAAAGGG AATATTACTC CAGCTTTTTG GCGGGACGAA TAAAACTTTT GAAAAGGGTG GAAGTCCGCG ATATCGTGGA GATATCAACG TGTTGTTGTG CGGGGACCCA TCGACGTCAA AGTCTCAGAT CCTGCAATAT GTCCACAAAA TTGCACCCAG AGGCATTTAT ACCAGCGGTA AAGGATCATC AGCTGTTGGT CTGACAGCAT ATGTTACGCG GGATCCAGAA TCAAGGCAGT TAGTTCTTGA ATCAGGTGCC CTTGTTCTTT CAGACGGGGG CGTTTGCTGC ATTGATGAAT TTGACAAAAT GAACGAGGCT ACGCGGTCTG TGCTACATGA AGTTATGGAA CAACAGACTG TGTCCATAGC AAAAGCAGGG ATCATCACAA CACTGAACGC AAGAACCAGT ATTCTTGCTT CTGCTAATCC AATCGGAAGC AAGTACAATC CAAACTTACC CGTTCCTCAA AATATCGATT TACCGCCGAC TCTCCTATCA CGATTTGACT TGGTGTATCT AGTGTTGGAC CGGATTGACG AACAGAACGA TCGTCGCCTG GCGAAACATT TGGTGGGAAT GTACCTTGAG GATACTCCCG AGAATGCCAG CACTGAGGAG ATATTGGTAT GCCTT

Details for CoimA.01544.a.A1.GE30648

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	25kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDDVKKGIL LQLFGGTNKT FEKGGSPRYR GDINVLLCGD PSTSKSQILQ YVHKIAPRGI YTSGKGSSAV GLTAYVTRDP ESRQLVLESG ALVLSDGGVC CIDEFDKMNE ATRSVLHEVM EQQTVSIAKA GIITTLNART SILASANPIG SKYNPNLPVP QNIDLPPTLL SRFDLVYLVL DRIDEQNDRR LAKHLVGMYL EDTPENASTE EILVCL

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg acgtgaaaaa gggaatatta ctccagcttt ttggcgggac gaataaaact tttgaaaagg gtggaagtcc gcgatatcgt ggagatatca acgtgttgtt gtgcggggac ccatcgacgt caaagtctca gatcctgcaa tatgtccaca aaattgcacc cagaggcatt tataccagcg gtaaaggatc atcagctgtt ggtctgacag catatgttac gcgggatcca gaatcaaggc agttagttct tgaatcaggt gcccttgttc tttcagacgg gggcgtttgc tgcattgatg aatttgacaa aatgaacgag gctacgcggt ctgtgctaca tgaagttatg gaacaacaga ctgtgtccat agcaaaagca gggatcatca caacactgaa cgcaagaacc agtattcttg cttctgctaa tccaatcgga agcaagtaca atccaaactt acccgttcct caaaatatcg atttaccgcc gactctccta tcacgatttg acttggtgta tctagtgttg gaccggattg acgaacagaa cgatcgtcgc ctggcgaaac atttggtggg aatgtacctt gaggatactc ccgagaatgc cagcactgag gagatattgg tatgccttta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctganttgg ctgctgccac cgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgacgtg aaaaagggaa tattactcca gctttttggc gggacgaata aaacttttga aaagggtgga agtccgcgat atcgtggaga tatcaacgtg ttgttgtgcg gggacccatc gacgtcaaag tctcagatcc tgcaatatgt ccacaaaatt gcacccagag gcatttatac cagcggtaaa ggatcatcag ctgttggtct gacagcatat gttacgcggg atccagaatc aaggcagtta gttcttgaat caggtgccct tgttctttca gacgggggcg tttgctgcat tgatgaattt gacaaaatga acgaggctac gcggtctgtg ctacatgaag ttatggaaca acagactgtg tccatagcaa aagcagggat catcacaaca ctgaacgcaa gaaccagtat tcttgcttct gctaatccaa tcggaagcaa gtacaatcca aacttacccg ttcctcaaaa tatcgattta ccgccgactc tcctatcacg atttgacttg gtgtatctag tgttggaccg gattgacgaa cagaacgatc gtcgcctggc gaaacatttg gtgggaatgt accttgagga tactcccgag aatgccagca ctgaggagat attggtatgc cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa