CoimA.01523.b

Putative uncharacterized protein (PRMT7 homologue)

CENTER ID: CoimA.01523.b
ORGANISM: Coccidioides immitis RS
ASSOCIATED DISEASE: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 II

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.01523.b.D16.PD00116 full length 232 548
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CIMG_03417
RefSeq: XP_001243976.1
UniProt: Q1E1Z6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDSELPARP REAVDDVEDS SSESSPSECL DLTKDDGWED VEPDEEVNPV VDLFSDKVFP DTRSMLQHCK ENFNFDLVKV QKQLDPTISG LDFLGTIKLV NYIRSEVQSG NTKPDVSSSA PFEDDKYLKP VLEDDALLYT LEDISDGDED LGQQAANPTS RIRDLEEELS RLREEFVEYK NMVQRSLSKQ LGSEVENGSQ IPPNQNQNEH VTETRRYKDA EAGYFTSYAY NTIHESMLKD TVRTDSYRDF IYDNKSIFKD KVVLDVGCGS GILSMFCAKA GAKMVIAVDN SDIIDKARQI VYENGFGDVI KCIRGKIEEV VLPVKQVDVI VSEWMGYCLL FEAMLDSVLF ARDRYLAPGG LMVPSHATLR IAPIADSDFI DEHISFWNSV YGFKMSGMLE NVYDEVLIQT ISPSAMVADS ALFLSLPLHT ITVEELTFVK EFKVAITKDA DTLDGWLVWF DMFFMPSCES KLADNATPGE MKKSGYVAFT TGPDGKETHW QQGVFLINRG KHQERPLKKG QVIKGKIGYK KKEEQSRLLD IDIEWAVDEE PPVHQEWALQ
NT Sequence
atgacggact ccgaacttcc tgctcggcct cgcgaggccg tcgacgatgt cgaagatagc tccagtgaat cgagtcccag cgaatgtcta gatctcacaa aggacgatgg atgggaggat gttgagcctg atgaagaagt caatcctgtc gttgacctct ttagcgacaa ggtctttcca gacactcggt caatgcttca acactgcaaa gaaaatttca acttcgacct tgttaaggtg cagaagcagc ttgatcctac aatttcaggc ctagatttcc ttgggaccat aaagctcgtc aattatatcc ggtcggaagt ccaatcgggg aataccaagc cggatgtatc gtccagtgcc ccatttgagg atgacaagta ccttaagcca gtgctggaag atgacgcgct tctgtatact ctagaggata tatcggatgg cgatgaagac cttggtcaac aggccgcgaa tcctacctct agaataaggg atcttgaaga agagctcagt cgccttcggg aagagttcgt tgaatacaag aatatggtgc agagatcgct cagcaaacag cttgggagcg aagtcgaaaa cggctcgcag atccctccga accagaatca aaacgagcac gttaccgaga caaggagata taaggatgca gaagctgggt acttcacttc ttatgcctat aacacgatac atgaatcgat gttaaaggat actgttcgaa ctgattctta ccgcgatttc atttacgata ataaaagtat ctttaaggat aaagtcgtac tggatgtggg ctgcggatct gggatcttgt ctatgttctg tgcaaaggcc ggagcaaaga tggttatagc cgttgacaat tcagatatca tcgacaaagc acgccaaatc gtgtatgaga atggattcgg cgacgtgatc aagtgtatcc ggggtaaaat tgaggaagta gtcttgcctg taaagcaagt tgacgttata gtctcggagt ggatgggata ctgtctccta tttgaggcga tgctcgactc agtgctcttt gcccgagatc gctatctcgc acctggagga ctaatggtgc cttcacatgc gacacttcgg atagcaccca ttgcagattc cgatttcatt gatgagcata tatcattctg gaattcagtt tatggattca agatgtcagg catgcttgag aacgtctatg atgaggttct catccaaaca atcagccctt ctgcaatggt tgcagattct gctttatttt tgtctctccc tttgcacact attacagttg aagagctcac gtttgtcaaa gagttcaaag ttgctataac aaaagatgcc gacaccttgg atggttggct tgtttggttt gacatgtttt tcatgccttc ttgtgaaagc aagcttgcag ataatgccac tccaggcgag atgaagaagt cgggctatgt ggcctttaca actggccctg atggaaaaga aacccattgg cagcaagggg tgtttttgat aaaccgtgga aaacaccaag agcgtccatt gaaaaaaggt caagtgatta agggaaaaat tggatacaaa aagaaggaag aacagtcgcg gttattggat atcgacatcg aatgggcagt tgatgaagag cctccggttc accaggaatg ggcattgcag tga
Details for CoimA.01523.b.D16.PD00116
PURIFICATION DATe: 3/9/2010
CONCENTRATION: 5.45mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Protocol Notes
notes unavailable
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG TIHESMLKDT VRTDSYRDFI YDNKSIFKDK VVLDVGCGSG ILSMFCAKAG AKMVIAVDNS DIIDKARQIV YENGFGDVIK CIRGKIEEVV LPVKQVDVIV SEWMGYCLLF EAMLDSVLFA RDRYLAPGGL MVPSHATLRI APIADSDFID EHISFWNSVY GFKMSGMLEN VYDEVLIQTI SPSAMVADSA LFLSLPLHTI TVEELTFVKE FKVAITKDAD TLDGWLVWFD MFFMPSCESK LADNATPGEM KKSGYVAFTT GPDGKETHWQ QGVFLINRGK HQERPLKKGQ VIKGKIGYKK KEEQSRLLDI DIEWAVD
Validated NT Sequence
taatccactg cccattcgat gtcgatgtcg aggagacgag actgttcctc ctttttctta tagccaatct tgcctttgat tacctggcct ttcttcagcg gacgctcttg gtgcttacca cggttaatga ggaagacacc ttgctgccag tgagtttctt taccatcagg accggtagtg aaagctacat aaccagactt cttcatttcg ccaggagttg cgttgtctgc cagtttagac tcgcatgaag gcatgaagaa catgtcgaac caaactaacc agccgtccag agtatcggcg tctttagtaa tagcaacctt gaattcttta acgaaggtaa gttcctcaac ggtgatagtg tggagaggga ggctcaggaa cagtgcggag tcagctacca ttgcggacgg agagatggtt tggatcagaa cttcgtcgta aacattctcc agcatgccgc tcatcttgaa gccgtaaaca gagttccaga aggagatgtg ttcatcgata aagtcgctat ctgcaatcgg agcaatgcgc agggttgcgt ggctaggaac catgagacca ccaggagcta aataacgatc gcgtgcgaac agcacggagt ccagcatagc ttcaaagagc aggcagtagc ccatccattc gctaacaatt acgtctactt gcttaaccgg cagtaccact tcttcaatct tgccacggat gcatttgata acgtcaccga agccattctc ataaactatc tgacgggctt tatcgatgat atcggagttg tcaactgcaa taaccatctt tgcaccggct ttagcgcaga acatgctcag gatacccgag ccacagccta cgtcaagaac tactttatcc ttaaagatgc tcttgttatc gtagatgaaa tcgcggtagg aatcagtacg cacggtatcc ttcagcatcg actcatggat ggttccaccg atttgttcac ggtgtgcttc gataatgtcg ttatcttcca tgtccagatc ttccggagtc tggtctgcct gaatacggat accgtcgtac agaaagcgca gggaatccat ttccttgccc tggcgtttag cgaatgcttc catcaggcga cgcagcgggg tggtcttctt aatcttgaag aagatttcgc tgctaccgtc gctaaccttc aggttgatgt gggtctccgg cttcacttcc ggttttactt cgccgcta
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGTIHE SMLKDTVRTD SYRDFIYDNK SIFKDKVVLD VGCGSGILSM FCAKAGAKMV IAVDNSDIID KARQIVYENG FGDVIKCIRG KIEEVVLPVK QVDVIVSEWM GYCLLFEAML DSVLFARDRY LAPGGLMVPS HATLRIAPIA DSDFIDEHIS FWNSVYGFKM SGMLENVYDE VLIQTISPSA MVADSALFLS LPLHTITVEE LTFVKEFKVA ITKDADTLDG WLVWFDMFFM PSCESKLADN ATPGEMKKSG YVAFTTGPDG KETHWQQGVF LINRGKHQER PLKKGQVIKG KIGYKKKEEQ SRLLDIDIEW AVD
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAC CATCCATGAG TCGATGCTGA AGGATACCGT GCGTACTGAT TCCTACCGCG ATTTCATCTA CGATAACAAG AGCATCTTTA AGGATAAAGT AGTTCTTGAC GTAGGCTGTG GCTCGGGTAT CCTGAGCATG TTCTGCGCTA AAGCCGGTGC AAAGATGGTT ATTGCAGTTG ACAACTCCGA TATCATCGAT AAAGCCCGTC AGATAGTTTA TGAGAATGGC TTCGGTGACG TTATCAAATG CATCCGTGGC AAGATTGAAG AAGTGGTACT GCCGGTTAAG CAAGTAGACG TAATTGTTAG CGAATGGATG GGCTACTGCC TGCTCTTTGA AGCTATGCTG GACTCCGTGC TGTTCGCACG CGATCGTTAT TTAGCTCCTG GTGGTCTCAT GGTTCCTAGC CACGCAACCC TGCGCATTGC TCCGATTGCA GATAGCGACT TTATCGATGA ACACATCTCC TTCTGGAACT CTGTTTACGG CTTCAAGATG AGCGGCATGC TGGAGAATGT TTACGACGAA GTTCTGATCC AAACCATCTC TCCGTCCGCA ATGGTAGCTG ACTCCGCACT GTTCCTGAGC CTCCCTCTCC ACACTATCAC CGTTGAGGAA CTTACCTTCG TTAAAGAATT CAAGGTTGCT ATTACTAAAG ACGCCGATAC TCTGGACGGC TGGTTAGTTT GGTTCGACAT GTTCTTCATG CCTTCATGCG AGTCTAAACT GGCAGACAAC GCAACTCCTG GCGAAATGAA GAAGTCTGGT TATGTAGCTT TCACTACCGG TCCTGATGGT AAAGAAACTC ACTGGCAGCA AGGTGTCTTC CTCATTAACC GTGGTAAGCA CCAAGAGCGT CCGCTGAAGA AAGGCCAGGT AATCAAAGGC AAGATTGGCT ATAAGAAAAA GGAGGAACAG TCTCGTCTCC TCGACATCGA CATCGAATGG GCAGTGGATT AAAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT