CoimA.01523.a

Putative uncharacterized protein (PRMT7 homologue)

CENTER ID:	CoimA.01523.a
ORGANISM:	Coccidioides immitis RS
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.01523.a.D11.GE082212a	full length		1	350

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CIMG_03531
RefSeq:	XP_001244090.1
UniProt:	Q1E1N2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNGAQKATAA AEMTSSADRM VGMDHAEVRY FTSYDHHGIH EEMLKDEVRT RSYRDAIYQN RHIFKDKVVL DVGCGTGILS MFAVRAGAKH VIGVDMSSII EKARQIVEIN GMSNKITLLQ GKMEEVVLPF PKVDIIISEW MGYFLLYESM LDTVLYARDN YLVPGGKIFP DKATMYLAGI EDGEYKDEKI GFWDNVYGFD YSPMKEIALA EPLVDTVELK ALVTDPCPII TFDLYTVTPA DLAFKVPFNL TAKRNDFIHA LIAWFDIDFT ACHKQIRFST GPHAKYTHWK QTVFYINEVI TIEENECVTG YLSNRPNDKN KRDLDIQISY RFETPDENRY TQGSCEYKMC

NT Sequence

atgaacggag ctcagaaagc gacggcggca gcagagatga cttccagtgc tgacagaatg gttggaatgg atcacgctga agtacgctat ttcactagct atgatcatca tggaattcat gaggagatgc ttaaagacga ggttcgaacc cgctcatacc gcgacgctat ctaccaaaac agacatatct ttaaggacaa ggttgtcctt gatgttggct gcggcactgg tatcctcagc atgtttgccg tccgcgctgg agctaaacac gtcattggtg ttgatatgtc ctccatcatt gagaaggccc gtcaaattgt agaaattaac gggatgagca acaagatcac tcttctccag ggtaaaatgg aggaggttgt tctgccattc ccaaaggttg atataatcat ctcagagtgg atgggatact tcttgctcta tgagagcatg ctggataccg tcctctatgc tcgtgataat tatctcgttc cgggtggaaa gattttccct gataaagcca ccatgtacct ggctggaatc gaagatggcg agtataagga tgagaaaatc ggcttttggg acaatgtgta tggtttcgac tattcgccga tgaaagaaat tgcgctggcc gaaccacttg tcgacaccgt tgagctgaag gctcttgtaa cggatccttg tcccatcata acctttgatt tatacaccgt tactccggcg gatttagctt ttaaggtccc tttcaatctc actgcaaaga gaaatgactt cattcatgct ctcattgctt ggtttgacat tgacttcaca gcatgccata agcaaattcg attctcgacc ggcccacatg cgaagtacac tcactggaaa cagaccgttt tctacattaa cgaagttatt actatcgaag agaacgagtg cgtgaccggt taccttagca accgaccgaa tgacaaaaat aagcgtgatc ttgatatcca gatctcgtac agatttgaga caccagatga aaatcgctat acgcagggtt cttgtgaata taagatgtgc taa

Details for CoimA.01523.a.D11.GE082212a

HARVESTED ON:	2/15/2010
SEQUENCED ON:	9/19/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	60

Validated AA Sequence

MFAVRAGAKH XIGVDXSSII EKARQIVEIN GMSNKITLLQ GKMEEVVLPF PKVDIIISEW MGYFLLYESM LDTVLYARDN YLVPGGKIFP DKATMYLAGI EDGEYKDEKI GFWDNVYGFD YSPMKEIALA EPLVDTVELK ALVTDPCPII TFDLYTVTPA DLAFKVPFNL TAKRNDFIHA LIAWFDIDFT ACHKQIRFST GPHAKYTHWK QTVFYINEVI TIEENECVTG YLSNRPNDKN KRDLDIQISY RFETPDENRY TQGSCEYKMC

Validated NT Sequence

ttaacacatc ttgtattcgc aggaaccctg ggtgtagcga ttctcgtccg gagtttcaaa gcgatagctg atctgaatat ccaggtcgcg cttattctta tcgttaggac ggttagagag gtaaccggtc acgcactcat tctcttcgat ggtgatcact tcgttgatat aaaacacggt ctgtttccaa tgggtgtatt tagcgtgcgg accagtgcta aaacggattt gcttgtggca ggcagtaaag tcgatgtcaa accaggcgat cagagcgtgg atgaagtcgt tacgtttagc ggtcaggtta aatgggactt tgaaagctaa gtcagccgga gtcacagtgt acagatcgaa ggtgatgatc gggcaaggat cggtcaccag agctttcagt tcaacagtgt caaccagcgg ttcagctaat gcgatctcct tcatcggaga gtagtcgaag ccgtagacgt tatcccagaa accaatcttc tcgtccttat attcaccatc ttcgatacca gctaaataca tggtagcttt atccgggaaa atcttgccac cagggaccag gtagttatca cgagcgtaca acacggtgtc aagcatggac tcgtacagca ggaaatagcc catccactcg gaaatgataa tatcgacctt tgggaatggg agcacgactt cctccatttt accctgcagc agagtgatct tgttgctcat gccgttgatc tctacgatct ggcgtgcttt ctcgattata gaaganatgt ccacaccgat ancgtgttta gcgcctgcac gaactgcgaa catggacaga atgccagtac cacagccaac gtcaagaact actttgtcct taaagatgtg acggttctga naaatagcat cacggtagg

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNGA QKATAAAEMT SSADRMVGMD HAEVRYFTSY DHHGIHEEML KDEVRTRSYR DAIYQNRHIF KDKVVLDVGC GTGILSMFAV RAGAKHVIGV DMSSIIEKAR QIVEINGMSN KITLLQGKME EVVLPFPKVD IIISEWMGYF LLYESMLDTV LYARDNYLVP GGKIFPDKAT MYLAGIEDGE YKDEKIGFWD NVYGFDYSPM KEIALAEPLV DTVELKALVT DPCPIITFDL YTVTPADLAF KVPFNLTAKR NDFIHALIAW FDIDFTACHK QIRFSTGPHA KYTHWKQTVF YINEVITIEE NECVTGYLSN RPNDKNKRDL DIQISYRFET PDENRYTQGS CEYKMC

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaacggcgct cagaaagcaa ccgccgctgc tgaaatgacc tcttctgctg accgtatggt aggcatggac cacgcggaag ttcgctactt cacttcttac gaccaccacg gtatccatga agaaatgctg aaagatgaag ttcgtactcg ttcctaccgt gatgctattt atcagaaccg tcacatcttt aaggacaaag tagttcttga cgttggctgt ggtactggca ttctgtccat gttcgcagtt cgtgcaggcg ctaaacacgt tatcggtgtg gacatgtctt ctataatcga gaaagcacgc cagatcgtag agatcaacgg catgagcaac aagatcactc tgctgcaggg taaaatggag gaagtcgtgc tcccattccc aaaggtcgat attatcattt ccgagtggat gggctatttc ctgctgtacg agtccatgct tgacaccgtg ttgtacgctc gtgataacta cctggtccct ggtggcaaga ttttcccgga taaagctacc atgtatttag ctggtatcga agatggtgaa tataaggacg agaagattgg tttctgggat aacgtctacg gcttcgacta ctctccgatg aaggagatcg cattagctga accgctggtt gacactgttg aactgaaagc tctggtgacc gatccttgcc cgatcatcac cttcgatctg tacactgtga ctccggctga cttagctttc aaagtcccat ttaacctgac cgctaaacgt aacgacttca tccacgctct gatcgcctgg tttgacatcg actttactgc ctgccacaag caaatccgtt ttagcactgg tccgcacgct aaatacaccc attggaaaca gaccgtgttt tatatcaacg aagtgatcac catcgaagag aatgagtgcg tgaccggtta cctctctaac cgtcctaacg ataagaataa gcgcgacctg gatattcaga tcagctatcg ctttgaaact ccggacgaga atcgctacac ccagggttcc tgcgaataca agatgtgtta aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat