CoimA.01320.a
Macrophage binding protein
| CENTER ID: | CoimA.01320.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.01320.a.A1.GE27869 | Full length( CoimA.01320.a ) | 1 | 243 | ||
| CoimA.01320.a.A1.GE28293 | Full length( CoimA.01320.a ) | 1 | 243 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.01320.a.A1.PS00439 | Full length( CoimA.01320.a ) | 1 | 243 |
Structures
3N7T
DEPOSITED: 5/27/2010
DETERMINATION: XRay
CLONE: CoimA.01320.a.A1.GE27869
PROTEIN: CoimA.01320.a.A1.PS00439
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_03805 |
| OrthoMCL: | OG5_130793 |
| UniProt: | Q9HEU1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPLPRKALL AITSAHPPFW PDGKRTGLFF SEALHPFNEL TAAGFEVDVA SETGTFGWDE HSLTQEYLSK EDEKVLHSEH NHFMEKMNKQ VFKAGDLAPH DYGLMFVCGG HGALYDFPHA KHLQNIAQDI YKRGGVIGAV CHGPAMLPGI HDENGDSVIK DKTVTGFTTK GEIMIKVIDK MREDHLHTIA DMAQTANAEY VPPEDPWDDF CKVDGRIVTG ANPQSATNTA RDTIKVYEGI VNE
NT Sequence
ATGGCACCGC TTCCGCGAAA GGCTTTGCTT GCAATAACCA GTGCGCACCC GCCGTTCTGG CCGGACGGGA AGAGGACAGG GCTCTTTTTC TCTGAGGCCC TTCACCCGTT CAACGAACTT ACTGCGGCGG GCTTCGAAGT TGACGTTGCG TCCGAGACGG GAACATTTGG ATGGGATGAG CATTCTCTCA CCCAAGAATA CCTATCCAAA GAGGACGAAA AAGTCTTGCA CTCTGAGCAC AATCACTTTA TGGAGAAGAT GAACAAGCAG GTGTTCAAGG CCGGTGATTT AGCGCCACAC GATTACGGAC TGATGTTTGT GTGTGGAGGG CACGGGGCAT TATATGATTT CCCGCATGCC AAACATCTTC AAAATATTGC GCAAGACATC TACAAACGCG GTGGGGTCAT TGGTGCAGTG TGTCACGGTC CAGCTATGCT ACCAGGAATC CATGACGAGA ATGGCGACTC GGTCATTAAG GACAAGACTG TCACTGGGTT CACAACAAAA GGCGAGATCA TGATTAAGGT CATCGATAAG ATGCGGGAAG ATCATCTCCA TACTATCGCC GATATGGCTC AGACCGCCAA CGCCGAATAT GTTCCTCCCG AAGACCCCTG GGACGACTTC TGCAAAGTCG ACGGCAGGAT CGTCACGGGT GCAAACCCGC AGAGCGCAAC AAATACGGCT AGGGATACGA TCAAAGTGTA CGAAGGCATT GTGAACGAA
Details for CoimA.01320.a.A1.GE27869
| HARVESTED ON: | 11/3/2009 |
| SEQUENCED ON: | 11/10/2009 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPLPRKAL LAITSAHPPF WPDGKRTGLF FSEALHPFNE LTAAGFEVDV ASETGTFGWD EHSLTQEYLS KEDEKVLHSE HNHFMEKMNK QVFKAGDLAP HDYGLMFVCG GHGALYDFPH AKHLQNIAQD IYKRGGVIGA VCHGPAMLPG IHDENGDSVI KDKTVTGFTT KGEIMIKVID KMREDHLHTI ADMAQTANAE YVPPEDPWDD FCKVDGRIVT GANPQSATNT ARDTIKVYEG IV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac cgcttccgcg aaaggctttg cttgcaataa ccagtgcgca cccgccgttc tggccggacg ggaagaggac agggctcttt ttctctgagg cccttcaccc gttcaacgaa cttactgcgg cgggcttcga agttgacgtt gcgtccgaga cgggaacatt tggatgggat gagcattctc tcacccaaga atacctatcc aaagaggacg aaaaagtctt gcactctgag cacaatcact ttatggagaa gatgaacaag caggtgttca aggccggtga tttagcgcca cacgattacg gactgatgtt tgtgtgtgga gggcacgggg cattatatga tttcccgcat gccaaacatc ttcaaaatat tgcgcaagac atctacaaac gcggtggggt cattggtgca gtgtgtcacg gtccagctat gctaccagga atccatgacg agaatggcga ctcggtcatt aaggacaaga ctgtcactgg gttcacaaca aaaggcgaga tcatgattaa ggtcatcgat aagatgcggg aagatcatct ccatactatc gccgatatgg ctcagaccgc caacgccgaa tatgttcctc ccgaagaccc ctgggacgac ttctgcaaag tcgacggcag gatcgtcacg ggtgcaaacc cgcagagcgc aacaaatacg gctagggata cgatcaaagt gtacgaaggc attgtga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPLPRKAL LAITSAHPPF WPDGKRTGLF FSEALHPFNE LTAAGFEVDV ASETGTFGWD EHSLTQEYLS KEDEKVLHSE HNHFMEKMNK QVFKAGDLAP HDYGLMFVCG GHGALYDFPH AKHLQNIAQD IYKRGGVIGA VCHGPAMLPG IHDENGDSVI KDKTVTGFTT KGEIMIKVID KMREDHLHTI ADMAQTANAE YVPPEDPWDD FCKVDGRIVT GANPQSATNT ARDTIKVYEG IVNE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaccgctt ccgcgaaagg ctttgcttgc aataaccagt gcgcacccgc cgttctggcc ggacgggaag aggacagggc tctttttctc tgaggccctt cacccgttca acgaacttac tgcggcgggc ttcgaagttg acgttgcgtc cgagacggga acatttggat gggatgagca ttctctcacc caagaatacc tatccaaaga ggacgaaaaa gtcttgcact ctgagcacaa tcactttatg gagaagatga acaagcaggt gttcaaggcc ggtgatttag cgccacacga ttacggactg atgtttgtgt gtggagggca cggggcatta tatgatttcc cgcatgccaa acatcttcaa aatattgcgc aagacatcta caaacgcggt ggggtcattg gtgcagtgtg tcacggtcca gctatgctac caggaatcca tgacgagaat ggcgactcgg tcattaagga caagactgtc actgggttca caacaaaagg cgagatcatg attaaggtca tcgataagat gcgggaagat catctccata ctatcgccga tatggctcag accgccaacg ccgaatatgt tcctcccgaa gacccctggg acgacttctg caaagtcgac ggcaggatcg tcacgggtgc aaacccgcag agcgcaacaa atacggctag ggatacgatc aaagtgtacg aaggcattgt gaacgaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for CoimA.01320.a.A1.GE28293
| HARVESTED ON: | 1/5/2010 |
| SEQUENCED ON: | 1/15/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPLPRKAL LAITSAHPPF WPDGKRTGLF FSEALHPFNE LTAAGFEVDV ASETGTFGWD EHSLTQEYLS KEDEKVLHSE HNHFMEKMNK QVFKAGDLAP HDYGLMFVCG GHGALYDFPH AKHLQNIAQD IYKRGGVIGA VCHGPAMLPG IHDENGDSVI KDKTVTGFTT KGEIMIKVID KMREDHLHTI ADMAQTANAE YVPPEDPWDD FCKVDGRIVT GANPQSATNT ARDTIKVYEG IV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac cgcttccgcg aaaggctttg cttgcaataa ccagtgcgca cccgccgttc tggccggacg ggaagaggac agggctcttt ttctctgagg cccttcaccc gttcaacgaa cttactgcgg cgggcttcga agttgacgtt gcgtccgaga cgggaacatt tggatgggat gagcattctc tcacccaaga atacctatcc aaagaggacg aaaaagtctt gcactctgag cacaatcact ttatggagaa gatgaacaag caggtgttca aggccggtga tttagcgcca cacgattacg gactgatgtt tgtgtgtgga gggcacgggg cattatatga tttcccgcat gccaaacatc ttcaaaatat tgcgcaagac atctacaaac gcggtggggt cattggtgca gtgtgtcacg gtccagctat gctaccagga atccatgacg agaatggcga ctcggtcatt aaggacaaga ctgtcactgg gttcacaaca aaaggcgaga tcatgattaa ggtcatcgat aagatgcggg aagatcatct ccatactatc gccgatatgg ctcagaccgc caacgccgaa tatgttcctc ccgaagaccc ctgggacgac ttctgcaaag tcgacggcag gatcgtcacg ggtgcaaacc cgcagagcgc aacaaatacg gctagggata cgatcaaagt gtacgaaggc attgtga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPLPRKAL LAITSAHPPF WPDGKRTGLF FSEALHPFNE LTAAGFEVDV ASETGTFGWD EHSLTQEYLS KEDEKVLHSE HNHFMEKMNK QVFKAGDLAP HDYGLMFVCG GHGALYDFPH AKHLQNIAQD IYKRGGVIGA VCHGPAMLPG IHDENGDSVI KDKTVTGFTT KGEIMIKVID KMREDHLHTI ADMAQTANAE YVPPEDPWDD FCKVDGRIVT GANPQSATNT ARDTIKVYEG IVNE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaccgctt ccgcgaaagg ctttgcttgc aataaccagt gcgcacccgc cgttctggcc ggacgggaag aggacagggc tctttttctc tgaggccctt cacccgttca acgaacttac tgcggcgggc ttcgaagttg acgttgcgtc cgagacggga acatttggat gggatgagca ttctctcacc caagaatacc tatccaaaga ggacgaaaaa gtcttgcact ctgagcacaa tcactttatg gagaagatga acaagcaggt gttcaaggcc ggtgatttag cgccacacga ttacggactg atgtttgtgt gtggagggca cggggcatta tatgatttcc cgcatgccaa acatcttcaa aatattgcgc aagacatcta caaacgcggt ggggtcattg gtgcagtgtg tcacggtcca gctatgctac caggaatcca tgacgagaat ggcgactcgg tcattaagga caagactgtc actgggttca caacaaaagg cgagatcatg attaaggtca tcgataagat gcgggaagat catctccata ctatcgccga tatggctcag accgccaacg ccgaatatgt tcctcccgaa gacccctggg acgacttctg caaagtcgac ggcaggatcg tcacgggtgc aaacccgcag agcgcaacaa atacggctag ggatacgatc aaagtgtacg aaggcattgt gaacgaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for CoimA.01320.a.A1.PS00439
| PURIFICATION DATe: | 12/31/2009 |
| CONCENTRATION: | 32.14mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPLPRKAL LAITSAHPPF WPDGKRTGLF FSEALHPFNE LTAAGFEVDV ASETGTFGWD EHSLTQEYLS KEDEKVLHSE HNHFMEKMNK QVFKAGDLAP HDYGLMFVCG GHGALYDFPH AKHLQNIAQD IYKRGGVIGA VCHGPAMLPG IHDENGDSVI KDKTVTGFTT KGEIMIKVID KMREDHLHTI ADMAQTANAE YVPPEDPWDD FCKVDGRIVT GANPQSATNT ARDTIKVYEG IV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac cgcttccgcg aaaggctttg cttgcaataa ccagtgcgca cccgccgttc tggccggacg ggaagaggac agggctcttt ttctctgagg cccttcaccc gttcaacgaa cttactgcgg cgggcttcga agttgacgtt gcgtccgaga cgggaacatt tggatgggat gagcattctc tcacccaaga atacctatcc aaagaggacg aaaaagtctt gcactctgag cacaatcact ttatggagaa gatgaacaag caggtgttca aggccggtga tttagcgcca cacgattacg gactgatgtt tgtgtgtgga gggcacgggg cattatatga tttcccgcat gccaaacatc ttcaaaatat tgcgcaagac atctacaaac gcggtggggt cattggtgca gtgtgtcacg gtccagctat gctaccagga atccatgacg agaatggcga ctcggtcatt aaggacaaga ctgtcactgg gttcacaaca aaaggcgaga tcatgattaa ggtcatcgat aagatgcggg aagatcatct ccatactatc gccgatatgg ctcagaccgc caacgccgaa tatgttcctc ccgaagaccc ctgggacgac ttctgcaaag tcgacggcag gatcgtcacg ggtgcaaacc cgcagagcgc aacaaatacg gctagggata cgatcaaagt gtacgaaggc attgtga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPLPRKAL LAITSAHPPF WPDGKRTGLF FSEALHPFNE LTAAGFEVDV ASETGTFGWD EHSLTQEYLS KEDEKVLHSE HNHFMEKMNK QVFKAGDLAP HDYGLMFVCG GHGALYDFPH AKHLQNIAQD IYKRGGVIGA VCHGPAMLPG IHDENGDSVI KDKTVTGFTT KGEIMIKVID KMREDHLHTI ADMAQTANAE YVPPEDPWDD FCKVDGRIVT GANPQSATNT ARDTIKVYEG IVNE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACCGCTT CCGCGAAAGG CTTTGCTTGC AATAACCAGT GCGCACCCGC CGTTCTGGCC GGACGGGAAG AGGACAGGGC TCTTTTTCTC TGAGGCCCTT CACCCGTTCA ACGAACTTAC TGCGGCGGGC TTCGAAGTTG ACGTTGCGTC CGAGACGGGA ACATTTGGAT GGGATGAGCA TTCTCTCACC CAAGAATACC TATCCAAAGA GGACGAAAAA GTCTTGCACT CTGAGCACAA TCACTTTATG GAGAAGATGA ACAAGCAGGT GTTCAAGGCC GGTGATTTAG CGCCACACGA TTACGGACTG ATGTTTGTGT GTGGAGGGCA CGGGGCATTA TATGATTTCC CGCATGCCAA ACATCTTCAA AATATTGCGC AAGACATCTA CAAACGCGGT GGGGTCATTG GTGCAGTGTG TCACGGTCCA GCTATGCTAC CAGGAATCCA TGACGAGAAT GGCGACTCGG TCATTAAGGA CAAGACTGTC ACTGGGTTCA CAACAAAAGG CGAGATCATG ATTAAGGTCA TCGATAAGAT GCGGGAAGAT CATCTCCATA CTATCGCCGA TATGGCTCAG ACCGCCAACG CCGAATATGT TCCTCCCGAA GACCCCTGGG ACGACTTCTG CAAAGTCGAC GGCAGGATCG TCACGGGTGC AAACCCGCAG AGCGCAACAA ATACGGCTAG GGATACGATC AAAGTGTACG AAGGCATTGT GAACGAAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA