CoimA.01291.a

Putative uncharacterized protein

CENTER ID: CoimA.01291.a
ORGANISM: Coccidioides immitis RS
ASSOCIATED DISEASE: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.01291.a.A1.GE30646 Full length( CoimA.01291.a ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.01291.a.A1.PW30520 Full length( CoimA.01291.a ) 1 253

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CIMG_07499
UniProt: Q1DQB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQTENSTA SEPVVTLAPA PAPLTVAPEL TPPLTSNNTT PRFSEKDVSV IFVLGGPGSG KGTQSANLVR EYGFSHLSAG DLLRAEQNRE ESQYGELIRN YIREGLIVPM EITVALLSNA MADILAEKKA QNQLIPGIPS RFLIDGFPRK MDQAIFFEET VVPSMATLFL SCPEEIMLNR LLKRGETSGR SDDNIDSIRK RFRVFVDQSM PVVDHYTKEG KVMSVSSVGS VDEVYSGIKR EIEARGIRPV QEN
NT Sequence
ATGTCGAGCC AAACTGAAAA CTCTACGGCC TCCGAGCCCG TAGTCACTCT TGCTCCCGCT CCCGCTCCGT TGACCGTCGC GCCCGAACTA ACCCCTCCAT TAACTTCGAA CAACACCACT CCTAGATTCT CAGAAAAAGA CGTGTCGGTG ATATTCGTCC TCGGCGGTCC CGGCAGTGGA AAAGGAACCC AGTCCGCCAA CCTCGTCCGG GAATATGGGT TCTCCCACCT ATCGGCGGGC GATCTCCTGC GCGCAGAGCA AAACCGTGAA GAGAGCCAAT ACGGCGAACT GATCCGGAAC TATATTCGTG AGGGTCTCAT CGTGCCCATG GAAATCACCG TCGCTCTACT TTCCAACGCA ATGGCCGACA TTCTCGCGGA GAAAAAGGCG CAGAACCAGC TTATTCCCGG CATACCATCA CGGTTCCTGA TCGATGGGTT CCCGCGCAAA ATGGACCAGG CGATCTTTTT CGAAGAGACT GTAGTCCCAT CAATGGCTAC TTTGTTTCTC TCGTGCCCGG AAGAAATCAT GCTCAACCGC CTGCTGAAGC GCGGGGAAAC AAGTGGTCGT TCAGACGATA ACATCGACTC GATCCGTAAG CGCTTCCGGG TCTTTGTGGA CCAGAGCATG CCTGTGGTCG ATCATTATAC GAAAGAGGGC AAGGTCATGA GTGTTTCCTC TGTTGGATCT GTAGATGAGG TGTACAGCGG GATCAAGAGG GAGATTGAAG CGCGGGGGAT CCGGCCTGTG CAAGAGAAT
Details for CoimA.01291.a.A1.GE30646
HARVESTED ON: 10/27/2010
SEQUENCED ON: 11/3/2010
EXPECTED MW: 30kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQTENST ASEPVVTLAP APAPLTVAPE LTPPLTSNNT TPRFSEKDVS VIFVLGGPGS GKGTQSANLV REYGFSHLSA GDLLRAEQNR EESQYGELIR NYIREGLIVP MEITVALLSN AMADILAEKK AQNQLIPGIP SRFLIDGFPR KMDQAIFFEE TVVPSMATLF LSCPEEIMLN RLLKRGETSG RSDDNIDSIR KRFRVFVGQS MPVVDHYTKE GKVMSVSSVG SVDEVYSGIK REIEARGIRP VQ
Validated NT Sequence
cttgcacagg ccggatcccc cgcgcttcaa tctccctctt gatcccgctg tacacctcat ctacagatcc aacagaggaa acactcatga ccttgccctc tttcgtataa tgatcgacca caggcatgct ctggcctaca aagacccgga agcgcttacg gatcgagtcg atgttatcgt ctgaacgacc acttgtttcc ccgcgcttca gcaggcggtt gagcatgatt tcttccgggc acgagagaaa caaagtagcc attgatggga ctacagtctc ttcgaaaaag atcgcctggt ccattttgcg cgggaaccca tcgatcagga accgtgatgg tatgccggga ataagctggt tctgcgcctt tttctccgcg agaatgtcgg ccattgcgtt ggaaagtaga gcgacggtga tttccatggg cacgatgaga ccctcacgaa tatagttccg gatcagttcg ccgtattggc tctcttcacg gttttgctct gcgcgcagga gatcgcccgc cgataggtgg gagaacccat attcccggac gaggttggcg gactgggttc cttttccact gccgggaccg ccgaggacga atatcaccga cacgtctttt tctgagaatc taggagtggt gttgttcgaa gttaatggag gggttagttc gggcgcgacg gtcaacggag cgggagcggg agcaagagtg actacgggct cggaggccgt agagttttca gtttggctcg acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQTENST ASEPVVTLAP APAPLTVAPE LTPPLTSNNT TPRFSEKDVS VIFVLGGPGS GKGTQSANLV REYGFSHLSA GDLLRAEQNR EESQYGELIR NYIREGLIVP MEITVALLSN AMADILAEKK AQNQLIPGIP SRFLIDGFPR KMDQAIFFEE TVVPSMATLF LSCPEEIMLN RLLKRGETSG RSDDNIDSIR KRFRVFVDQS MPVVDHYTKE GKVMSVSSVG SVDEVYSGIK REIEARGIRP VQEN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgagccaa actgaaaact ctacggcctc cgagcccgta gtcactcttg ctcccgctcc cgctccgttg accgtcgcgc ccgaactaac ccctccatta acttcgaaca acaccactcc tagattctca gaaaaagacg tgtcggtgat attcgtcctc ggcggtcccg gcagtggaaa aggaacccag tccgccaacc tcgtccggga atatgggttc tcccacctat cggcgggcga tctcctgcgc gcagagcaaa accgtgaaga gagccaatac ggcgaactga tccggaacta tattcgtgag ggtctcatcg tgcccatgga aatcaccgtc gctctacttt ccaacgcaat ggccgacatt ctcgcggaga aaaaggcgca gaaccagctt attcccggca taccatcacg gttcctgatc gatgggttcc cgcgcaaaat ggaccaggcg atctttttcg aagagactgt agtcccatca atggctactt tgtttctctc gtgcccggaa gaaatcatgc tcaaccgcct gctgaagcgc ggggaaacaa gtggtcgttc agacgataac atcgactcga tccgtaagcg cttccgggtc tttgtggacc agagcatgcc tgtggtcgat cattatacga aagagggcaa ggtcatgagt gtttcctctg ttggatctgt agatgaggtg tacagcggga tcaagaggga gattgaagcg cgggggatcc ggcctgtgca agagaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for CoimA.01291.a.A1.PW30520
PURIFICATION DATe: 12/28/2010
CONCENTRATION: 24.94mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQTENST ASEPVVTLAP APAPLTVAPE LTPPLTSNNT TPRFSEKDVS VIFVLGGPGS GKGTQSANLV REYGFSHLSA GDLLRAEQNR EESQYGELIR NYIREGLIVP MEITVALLSN AMADILAEKK AQNQLIPGIP SRFLIDGFPR KMDQAIFFEE TVVPSMATLF LSCPEEIMLN RLLKRGETSG RSDDNIDSIR KRFRVFVGQS MPVVDHYTKE GKVMSVSSVG SVDEVYSGIK REIEARGIRP VQ
Validated NT Sequence
cttgcacagg ccggatcccc cgcgcttcaa tctccctctt gatcccgctg tacacctcat ctacagatcc aacagaggaa acactcatga ccttgccctc tttcgtataa tgatcgacca caggcatgct ctggcctaca aagacccgga agcgcttacg gatcgagtcg atgttatcgt ctgaacgacc acttgtttcc ccgcgcttca gcaggcggtt gagcatgatt tcttccgggc acgagagaaa caaagtagcc attgatggga ctacagtctc ttcgaaaaag atcgcctggt ccattttgcg cgggaaccca tcgatcagga accgtgatgg tatgccggga ataagctggt tctgcgcctt tttctccgcg agaatgtcgg ccattgcgtt ggaaagtaga gcgacggtga tttccatggg cacgatgaga ccctcacgaa tatagttccg gatcagttcg ccgtattggc tctcttcacg gttttgctct gcgcgcagga gatcgcccgc cgataggtgg gagaacccat attcccggac gaggttggcg gactgggttc cttttccact gccgggaccg ccgaggacga atatcaccga cacgtctttt tctgagaatc taggagtggt gttgttcgaa gttaatggag gggttagttc gggcgcgacg gtcaacggag cgggagcggg agcaagagtg actacgggct cggaggccgt agagttttca gtttggctcg acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQTENST ASEPVVTLAP APAPLTVAPE LTPPLTSNNT TPRFSEKDVS VIFVLGGPGS GKGTQSANLV REYGFSHLSA GDLLRAEQNR EESQYGELIR NYIREGLIVP MEITVALLSN AMADILAEKK AQNQLIPGIP SRFLIDGFPR KMDQAIFFEE TVVPSMATLF LSCPEEIMLN RLLKRGETSG RSDDNIDSIR KRFRVFVDQS MPVVDHYTKE GKVMSVSSVG SVDEVYSGIK REIEARGIRP VQEN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGAGCCAA ACTGAAAACT CTACGGCCTC CGAGCCCGTA GTCACTCTTG CTCCCGCTCC CGCTCCGTTG ACCGTCGCGC CCGAACTAAC CCCTCCATTA ACTTCGAACA ACACCACTCC TAGATTCTCA GAAAAAGACG TGTCGGTGAT ATTCGTCCTC GGCGGTCCCG GCAGTGGAAA AGGAACCCAG TCCGCCAACC TCGTCCGGGA ATATGGGTTC TCCCACCTAT CGGCGGGCGA TCTCCTGCGC GCAGAGCAAA ACCGTGAAGA GAGCCAATAC GGCGAACTGA TCCGGAACTA TATTCGTGAG GGTCTCATCG TGCCCATGGA AATCACCGTC GCTCTACTTT CCAACGCAAT GGCCGACATT CTCGCGGAGA AAAAGGCGCA GAACCAGCTT ATTCCCGGCA TACCATCACG GTTCCTGATC GATGGGTTCC CGCGCAAAAT GGACCAGGCG ATCTTTTTCG AAGAGACTGT AGTCCCATCA ATGGCTACTT TGTTTCTCTC GTGCCCGGAA GAAATCATGC TCAACCGCCT GCTGAAGCGC GGGGAAACAA GTGGTCGTTC AGACGATAAC ATCGACTCGA TCCGTAAGCG CTTCCGGGTC TTTGTGGACC AGAGCATGCC TGTGGTCGAT CATTATACGA AAGAGGGCAA GGTCATGAGT GTTTCCTCTG TTGGATCTGT AGATGAGGTG TACAGCGGGA TCAAGAGGGA GATTGAAGCG CGGGGGATCC GGCCTGTGCA AGAGAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA