SSGCID: Target Page

CoimA.01173.a

Putative uncharacterized protein

CENTER ID:	CoimA.01173.a
ORGANISM:	Coccidioides immitis RS
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.01173.a.A1.GE27333	Full length( CoimA.01173.a )		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CIMG_07467
UniProt:	Q1DQE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDAIREVKYL QELSHPNVIA LHAVFSSKDR NLNLVLEYLP LGDLEMLIKD SAIQYGAADV KAWVGMLGRA VWFCHENFIL HRDIKPNNLL IASDGEVKLA DFGLARSFAD PRLNMTHQVI TRWYRPLELL YGARQYSGAV DVWSMGMVFA ELLLRVPFAA GNTDLDQISK ICAAFGTPTE DNWPGVTKLP NFVPVEKNQI VPLQGRDFFL RQFPTVGHLG ADLLSSMLKL DPRNRSTAKQ ILQHAWWSAE PRPTRKENLP RKAGGAKKMG NDLSRRGGVT DEGSFKNAAR RLDFGGLLPK

NT Sequence

ATGGATGCAA TACGCGAAGT GAAATATCTC CAGGAACTGT CTCATCCCAA CGTAATTGCT CTTCATGCCG TCTTCTCGTC CAAAGATCGA AATTTAAACT TGGTTTTGGA ATATTTACCG CTCGGTGATT TGGAGATGCT GATCAAAGAC AGCGCTATTC AGTACGGCGC TGCCGACGTC AAGGCTTGGG TGGGCATGCT CGGCCGCGCA GTCTGGTTTT GTCATGAGAA TTTCATCTTA CACAGGGATA TCAAACCAAA CAATCTGCTA ATAGCATCAG ACGGTGAAGT TAAGCTTGCG GACTTTGGAC TGGCCAGATC ATTCGCAGAC CCTCGCCTAA ATATGACTCA CCAGGTAATC ACGCGCTGGT ATAGGCCACT AGAGCTTCTT TACGGGGCGC GCCAGTACTC TGGCGCAGTA GATGTATGGT CTATGGGAAT GGTTTTTGCA GAGTTACTCC TCCGCGTCCC CTTCGCTGCA GGAAATACAG ACTTGGATCA GATATCCAAG ATCTGCGCCG CGTTTGGCAC ACCCACCGAG GATAACTGGC CGGGGGTAAC GAAATTGCCC AACTTTGTCC CCGTTGAAAA GAACCAAATT GTCCCATTGC AAGGGAGGGA TTTCTTCCTT CGGCAATTTC CCACCGTGGG ACACCTTGGA GCAGACTTGC TATCGTCGAT GTTAAAACTT GATCCACGAA ATCGCAGTAC TGCGAAGCAG ATATTACAAC ATGCGTGGTG GTCCGCGGAA CCCAGGCCAA CTAGAAAAGA AAATTTACCT CGCAAAGCTG GAGGTGCAAA GAAGATGGGC AATGATTTGA GCAGAAGAGG CGGCGTAACA GATGAAGGAT CTTTCAAGAA TGCGGCTCGT AGGCTTGATT TTGGAGGACT CCTACCGAAA

Details for CoimA.01173.a.A1.GE27333

HARVESTED ON:	10/23/2009
SEQUENCED ON:	10/29/2009
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MDAIREVKYL QELSHPNVIA LHAVFSSKDR NLNLVLEYLP LGDLEMLIKD SAIQYGAADV KAWVGMLGRA VWFCHENFIL HRDIKPNNLL IASDGEVKLA DFGLARSFAD PRLNMTHQVI TRWYRPLELL YGARQYSGAV DVWSMGMVFA ELLLRVPFAA GNTDLDQISX ICAAFGTPTE DNWPGVTKLP NFVPVEKNQI VPLQGRDFFL RQFPTVGHLG ADLLSSMLKL DPRNRSTAKQ ILQHAWWSAE PRPTRKENLP RKAGGAKKMG NDLSRRGGVT DEGSFKNAAR RLDFGGLLPK

Validated NT Sequence

ttattatttc ggtaggagtc ctccaaaatc aagcctacga gccgcattct tgaaagatcc ttcatctgtt acgccgcctc ttctgctcaa atcattgccc atcttctttg cacctccagc tttgcgaggt aaattttctt ttctagttgg cctgggttcc gcggaccacc acgcatgttg taatatctgc ttcgcagtac tgcgatttcg tggatcaagt tttaacatcg acgatagcaa gtctgctcca aggtgtccca cggtgggaaa ttgccgaagg aagaaatccc tcccttgcaa tgggacaatt tggttctttt caacggggac aaagttgggc aatttcgtta cccccggcca gttatcctcg gtgggtgtgc caaacgcggc gcagatctng gatatctgat ccaagtctgt atttcctgca gcgaagggga cgcggaggag taactctgca aaaaccattc ccatagacca tacatctact gcgccagagt actggcgcgc cccgtaaaga agctctagtg gcctatacca gcgcgtgatt acctggtgag tcatatttag gcgagggtct gcgaatgatc tggccagtcc aaagtccgca agcttaactt caccgtctga tgctattagc agattgtttg gtttgatatc cctgtgtaag atgaaattct catgacaaaa ccagactgcg cggccgagca tgcccaccca agccttgacg tcggcagcgc cgtactgaat agcgctgtct ttgatcagca tctccaaatc accgagcggt aaatattcca aaaccaagtt taaatttcga tctttggacg agaagacggc atgaagagca attacgttgg gatgagacag ttcctggaga tatttcactt cgcgtattgc atccatcgaa ccaggaccct gggtctgagc ttccagggta cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDAIREVKY LQELSHPNVI ALHAVFSSKD RNLNLVLEYL PLGDLEMLIK DSAIQYGAAD VKAWVGMLGR AVWFCHENFI LHRDIKPNNL LIASDGEVKL ADFGLARSFA DPRLNMTHQV ITRWYRPLEL LYGARQYSGA VDVWSMGMVF AELLLRVPFA AGNTDLDQIS KICAAFGTPT EDNWPGVTKL PNFVPVEKNQ IVPLQGRDFF LRQFPTVGHL GADLLSSMLK LDPRNRSTAK QILQHAWWSA EPRPTRKENL PRKAGGAKKM GNDLSRRGGV TDEGSFKNAA RRLDFGGLLP K

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatgcaata cgcgaagtga aatatctcca ggaactgtct catcccaacg taattgctct tcatgccgtc ttctcgtcca aagatcgaaa tttaaacttg gttttggaat atttaccgct cggtgatttg gagatgctga tcaaagacag cgctattcag tacggcgctg ccgacgtcaa ggcttgggtg ggcatgctcg gccgcgcagt ctggttttgt catgagaatt tcatcttaca cagggatatc aaaccaaaca atctgctaat agcatcagac ggtgaagtta agcttgcgga ctttggactg gccagatcat tcgcagaccc tcgcctaaat atgactcacc aggtaatcac gcgctggtat aggccactag agcttcttta cggggcgcgc cagtactctg gcgcagtaga tgtatggtct atgggaatgg tttttgcaga gttactcctc cgcgtcccct tcgctgcagg aaatacagac ttggatcaga tatccaagat ctgcgccgcg tttggcacac ccaccgagga taactggccg ggggtaacga aattgcccaa ctttgtcccc gttgaaaaga accaaattgt cccattgcaa gggagggatt tcttccttcg gcaatttccc accgtgggac accttggagc agacttgcta tcgtcgatgt taaaacttga tccacgaaat cgcagtactg cgaagcagat attacaacat gcgtggtggt ccgcggaacc caggccaact agaaaagaaa atttacctcg caaagctgga ggtgcaaaga agatgggcaa tgatttgagc agaagaggcg gcgtaacaga tgaaggatct ttcaagaatg cggctcgtag gcttgatttt ggaggactcc taccgaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa