CoimA.00871.a

Oxidoreductase

CENTER ID: CoimA.00871.a
ORGANISM: Coccidioides immitis RS
ASSOCIATED DISEASE: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 II

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00871.a.A1.PW29193 Full length( CoimA.00871.a ) 1 348

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CIMG_03135
UniProt: A0A0E1RWJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATENKALFY QKVPTEFPVP GEHLTIEKVP FDPEAPAPDG GLTLQALYTS FDPYMRGGMR PAQVKSYRPA YEIGKPLPSI SIVKVLKSNN DRFKPGDIAI GFMPIQQYIA APAETVQALR LLDNPLGLDD LRYFLGALGM PGLTAYSSLM EIGKPKKGET IFISSAAGAV GQVVGQIAKH EGLRVVGSVG SDEKLNYIIN DLGFDAGFNY KKEKPRDALK RLIPEGIDIY YENVGGEHLD AAIESMNDFG RIVTCGMISQ YNVKPEDRYP IKNLFMVVTK RITMRGFIVS DPGMGDKWAK EHRERVSQWI KDGTFKPMIH ETVGIDNAAE GLVGLFQGKN FGKAVLKL
NT Sequence
ATGGCTACAG AAAACAAGGC GTTGTTCTAT CAGAAGGTCC CCACCGAGTT TCCTGTACCC GGTGAGCACT TGACGATTGA GAAAGTGCCG TTTGACCCTG AAGCTCCAGC TCCCGATGGC GGTCTTACCC TTCAAGCGTT GTACACGAGC TTCGATCCAT ATATGCGGGG TGGCATGCGG CCGGCTCAAG TCAAATCCTA CCGTCCGGCA TACGAAATAG GAAAACCACT TCCTAGCATT TCGATCGTTA AGGTCCTAAA ATCGAACAAT GACAGGTTCA AACCAGGCGA CATTGCAATA GGATTCATGC CCATCCAACA GTATATCGCT GCACCCGCAG AAACTGTCCA GGCCCTTAGG CTGCTGGATA ACCCACTCGG GCTGGACGAC CTCCGCTACT TCCTTGGCGC TCTGGGGATG CCCGGCCTCA CCGCATATTC GTCCCTGATG GAGATTGGAA AGCCGAAGAA GGGAGAGACC ATTTTTATTT CGTCTGCGGC TGGAGCCGTG GGCCAGGTCG TTGGCCAGAT CGCGAAGCAC GAAGGTCTCA GGGTCGTTGG AAGTGTTGGG TCCGATGAAA AATTGAACTA CATTATTAAT GACCTTGGTT TTGACGCTGG ATTCAATTAT AAGAAGGAAA AGCCCCGTGA CGCTCTGAAG CGACTGATCC CCGAAGGCAT CGACATCTAC TACGAGAACG TGGGTGGCGA GCATCTGGAT GCGGCTATTG AGTCAATGAA TGACTTTGGA CGCATCGTTA CCTGTGGAAT GATTTCCCAG TATAACGTCA AGCCAGAGGA CCGGTACCCG ATCAAGAATC TTTTTATGGT AGTGACCAAG AGAATCACGA TGCGCGGCTT CATTGTATCT GATCCCGGAA TGGGAGACAA ATGGGCCAAG GAGCACCGGG AGCGTGTCTC CCAATGGATC AAAGATGGCA CCTTCAAACC TATGATCCAT GAGACCGTTG GCATCGACAA TGCTGCAGAG GGACTCGTGG GCTTGTTCCA GGGGAAGAAT TTCGGCAAGG CTGTGTTGAA ACTT
Details for CoimA.00871.a.A1.PW29193
PURIFICATION DATe: 3/22/2010
CONCENTRATION: 25.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATENKALF YQKVPTEFPV PGEHLTIEKV PFDPEAPAPD GGLTLQALYT SFDPYMRGGM RPAQVKSYRP AYEIGKPLPS ISIVKVLKSN NDRFKPGDIA IGFMPIQQYI AAPAETVQAL RLLDNPLGLD DLRYSLGALG MPGLTAYSSL MEIGKPKKGE TIFISSAAGA VGQVVGQIAK HEGLRVVGSV GSDEKLNYII NDLGFDAGFN YKKEKPRDAL KRLIPEGIDI YYENVGGEHL DAAIESMNDF GRIVTCGMIS QYNVKPEDRY PIKNLFMVVT KRITMRGFIV SDPGMGDKWA KEHRERVSQW IKDGTFKPMI HETVGIDNAA EGLVGLFQGK NFGKAVL
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggctaca gaaaacaagg cgttgttcta tcagaaggtc cccaccgagt ttcctgtacc cggtgagcac ttgacgattg agaaagtgcc gtttgaccct gaagctccag ctcccgatgg cggtcttacc cttcaagcgt tgtacacgag cttcgatcca tatatgcggg gtggcatgcg gccggctcaa gtcaaatcct accgtccggc atacgaaata ggaaaaccac ttcctagcat ttcgatcgtt aaggtcctaa aatcgaacaa tgacaggttc aaaccaggcg acattgcaat aggattcatg cccatccaac agtatatcgc tgcacccgca gaaactgtcc aggcccttag gctgctggat aacccactcg ggctggacga cctccgctac tcccttggcg ctctggggat gcccggcctc accgcatatt cgtccctgat ggagattgga aagccgaaga agggagagac catttttatt tcgtctgcgg ctggagccgt gggccaggtc gttggccaga tcgcgaagca cgaaggtctc agggtcgttg gaagtgttgg gtccgatgaa aaattgaact acattattaa tgaccttggt tttgacgctg gattcaatta taagaaggaa aagccccgtg acgctctgaa gcgactgatc cccgaaggca tcgacatcta ctacgagaac gtgggtggcg agcatctgga tgcggctatt gagtcaatga atgactttgg acgcatcgtt acctgtggaa tgatttccca gtataacgtc aagccagagg accggtaccc gatcaagaat ctttttatgg tagtgaccaa gagaatcacg atgcgcggct tcattgtatc tgatcccgga atgggagaca aatgggccaa ggagcaccgg gagcgtgtct cccaatggat caaagatggc accttcaaac ctatgatcca tgagaccgtt ggcatcgaca atgctgcaga gggactcgtg ggcttgttcc aggggaagaa tttcggcaag gctgtgttga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATENKALF YQKVPTEFPV PGEHLTIEKV PFDPEAPAPD GGLTLQALYT SFDPYMRGGM RPAQVKSYRP AYEIGKPLPS ISIVKVLKSN NDRFKPGDIA IGFMPIQQYI AAPAETVQAL RLLDNPLGLD DLRYFLGALG MPGLTAYSSL MEIGKPKKGE TIFISSAAGA VGQVVGQIAK HEGLRVVGSV GSDEKLNYII NDLGFDAGFN YKKEKPRDAL KRLIPEGIDI YYENVGGEHL DAAIESMNDF GRIVTCGMIS QYNVKPEDRY PIKNLFMVVT KRITMRGFIV SDPGMGDKWA KEHRERVSQW IKDGTFKPMI HETVGIDNAA EGLVGLFQGK NFGKAVLKL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTACAGAA AACAAGGCGT TGTTCTATCA GAAGGTCCCC ACCGAGTTTC CTGTACCCGG TGAGCACTTG ACGATTGAGA AAGTGCCGTT TGACCCTGAA GCTCCAGCTC CCGATGGCGG TCTTACCCTT CAAGCGTTGT ACACGAGCTT CGATCCATAT ATGCGGGGTG GCATGCGGCC GGCTCAAGTC AAATCCTACC GTCCGGCATA CGAAATAGGA AAACCACTTC CTAGCATTTC GATCGTTAAG GTCCTAAAAT CGAACAATGA CAGGTTCAAA CCAGGCGACA TTGCAATAGG ATTCATGCCC ATCCAACAGT ATATCGCTGC ACCCGCAGAA ACTGTCCAGG CCCTTAGGCT GCTGGATAAC CCACTCGGGC TGGACGACCT CCGCTACTTC CTTGGCGCTC TGGGGATGCC CGGCCTCACC GCATATTCGT CCCTGATGGA GATTGGAAAG CCGAAGAAGG GAGAGACCAT TTTTATTTCG TCTGCGGCTG GAGCCGTGGG CCAGGTCGTT GGCCAGATCG CGAAGCACGA AGGTCTCAGG GTCGTTGGAA GTGTTGGGTC CGATGAAAAA TTGAACTACA TTATTAATGA CCTTGGTTTT GACGCTGGAT TCAATTATAA GAAGGAAAAG CCCCGTGACG CTCTGAAGCG ACTGATCCCC GAAGGCATCG ACATCTACTA CGAGAACGTG GGTGGCGAGC ATCTGGATGC GGCTATTGAG TCAATGAATG ACTTTGGACG CATCGTTACC TGTGGAATGA TTTCCCAGTA TAACGTCAAG CCAGAGGACC GGTACCCGAT CAAGAATCTT TTTATGGTAG TGACCAAGAG AATCACGATG CGCGGCTTCA TTGTATCTGA TCCCGGAATG GGAGACAAAT GGGCCAAGGA GCACCGGGAG CGTGTCTCCC AATGGATCAA AGATGGCACC TTCAAACCTA TGATCCATGA GACCGTTGGC ATCGACAATG CTGCAGAGGG ACTCGTGGGC TTGTTCCAGG GGAAGAATTT CGGCAAGGCT GTGTTGAAAC TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA