SSGCID: Target Page

CoimA.00860.a

Putative uncharacterized protein

CENTER ID:	CoimA.00860.a
ORGANISM:	Coccidioides immitis RS
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.00860.a.A1.GE30643	Full length( CoimA.00860.a )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.00860.a.A1.PW30517	Full length( CoimA.00860.a )		1	268

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CIMG_08639
UniProt:	Q1DM24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSPSRASAL LANLSSVTSR ISAASVPGSS VRLVAVSKLK PASDILALYN PPTSHLHFGE NYLQELLEKS KILPPEIRWH FIGGLQSNKC VTLARDVRGL WAVESVDTQK KATLLDKGWG ERLKNHSAQQ QQQQQGRAEP ERLRVFVQVN TSGEESKSGV EPSQAVELCR FIREQCPRLK LEGLMTIGAI ARSKATTPET QNEDFDCLRE TRDRICQELG LEGEEKLELS MGMSEDFEGA IAMGSNEVRV GSTIFGARPP KGEAKVVP

NT Sequence

ATGCCCTCTC CGTCCCGCGC CTCCGCCTTG CTTGCGAACC TCTCCTCCGT GACCTCCCGC ATCTCCGCCG CCTCAGTCCC CGGCAGTTCT GTCCGCCTCG TCGCTGTCTC CAAACTCAAG CCCGCATCCG ACATCCTCGC CCTCTACAAC CCACCCACAT CCCACCTTCA TTTCGGTGAA AACTACCTGC AAGAGCTCCT CGAAAAATCC AAAATCCTGC CCCCGGAAAT CAGATGGCAC TTCATCGGCG GGCTACAGTC GAACAAATGC GTCACACTAG CGAGGGATGT GAGAGGTCTC TGGGCCGTCG AGAGTGTAGA CACACAGAAG AAAGCGACCC TGCTAGACAA AGGGTGGGGC GAGAGACTAA AGAATCACTC CGCACAGCAG CAGCAACAAC AACAGGGCAG AGCGGAGCCG GAGCGATTGC GTGTTTTTGT CCAGGTGAAC ACCTCCGGAG AAGAGAGTAA GTCTGGTGTT GAGCCATCAC AGGCGGTGGA ATTGTGCCGG TTTATCAGGG AGCAGTGTCC ACGGTTGAAG CTAGAGGGCC TAATGACTAT CGGGGCCATT GCTCGGTCCA AGGCAACAAC CCCGGAGACT CAGAATGAAG ATTTTGACTG TTTGAGAGAG ACAAGGGATA GAATTTGCCA AGAATTGGGT TTGGAAGGGG AGGAGAAGCT GGAGTTGAGC ATGGGTATGA GTGAAGACTT TGAGGGTGCT ATTGCCATGG GGAGCAATGA GGTTAGAGTG GGGAGCACGA TTTTCGGAGC TCGTCCTCCA AAAGGAGAAG CAAAAGTGGT TCCC

Details for CoimA.00860.a.A1.GE30643

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	92

Validated AA Sequence

MPSPSRASAL LANLSSVTSR ISAASXPGSS VRLVAVSKLK PASDILALYN PPTSHLHFGE NCLQELLEKS KILPPEIRWH FIGGLQSNXC VTLARDVRGL WAVESVDTQK KATLLDKGWG ERLKNHSAQQ QQQQQGRAEP ERLRVFVQVN TSGEESKSGV EPSQAVELCR FIREQCPRLK LEGLMTIGAI ARSKATTPET QNEDFDCLRE TRDRICQELG LEGEEKLELS MGMSEDFEGA IAMGSNEVRV GSTIFGARPP KGEAKVVP

Validated NT Sequence

ttattaggga accacttttg cttctccttt tggaggacga gctccgaaaa tcgtgctccc cactctaacc tcattgctcc ccatggcaat agcaccctca aagtcttcac tcatacccat gctcaactcc agcttctcct ccccttccaa acccaattct tggcaaattc tatcccttgt ctctctcaaa cagtcaaaat cttcattctg agtctccggg gttgttgcct tggaccgagc aatggccccg atagtcatta ggccctctag cttcaaccgt ggacactgct ccctgataaa ccggcacaat tccaccgcct gtgatggctc aacaccagac ttactctctt ctccggaggt gttcacctgg acaaaaacac gcaatcgctc cggctccgct ctgccctgtt gttgttgctg ctgctgtgcg gagtgattct ttagtctctc gccccaccct ttgtctagca gggtcgcttt cttctgtgtg tctacactct cgacggccca gagacctctc acatccctcg ctagtgtgac gcanttgttc gactgtagcc cgccgatgaa gtgccatctg atttccgggg gcaggatttt ggatttttcg aggagctctt gcaggcagtt ttcaccgaaa tgaaggtggg atgtgggtgg gttgtagagg gcgaggatgt cggatgcggg cttgagtttg gagacagcga cgaggcggac agaactgccg gggantgagg cggcggagat gcgggaggtc acggaggaga ggttcgcaag caaggcggag gcgcgggacg gagagggcat cgaaccnnga cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPSPSRASA LLANLSSVTS RISAASVPGS SVRLVAVSKL KPASDILALY NPPTSHLHFG ENYLQELLEK SKILPPEIRW HFIGGLQSNK CVTLARDVRG LWAVESVDTQ KKATLLDKGW GERLKNHSAQ QQQQQQGRAE PERLRVFVQV NTSGEESKSG VEPSQAVELC RFIREQCPRL KLEGLMTIGA IARSKATTPE TQNEDFDCLR ETRDRICQEL GLEGEEKLEL SMGMSEDFEG AIAMGSNEVR VGSTIFGARP PKGEAKVVP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccctctccg tcccgcgcct ccgccttgct tgcgaacctc tcctccgtga cctcccgcat ctccgccgcc tcagtccccg gcagttctgt ccgcctcgtc gctgtctcca aactcaagcc cgcatccgac atcctcgccc tctacaaccc acccacatcc caccttcatt tcggtgaaaa ctacctgcaa gagctcctcg aaaaatccaa aatcctgccc ccggaaatca gatggcactt catcggcggg ctacagtcga acaaatgcgt cacactagcg agggatgtga gaggtctctg ggccgtcgag agtgtagaca cacagaagaa agcgaccctg ctagacaaag ggtggggcga gagactaaag aatcactccg cacagcagca gcaacaacaa cagggcagag cggagccgga gcgattgcgt gtttttgtcc aggtgaacac ctccggagaa gagagtaagt ctggtgttga gccatcacag gcggtggaat tgtgccggtt tatcagggag cagtgtccac ggttgaagct agagggccta atgactatcg gggccattgc tcggtccaag gcaacaaccc cggagactca gaatgaagat tttgactgtt tgagagagac aagggataga atttgccaag aattgggttt ggaaggggag gagaagctgg agttgagcat gggtatgagt gaagactttg agggtgctat tgccatgggg agcaatgagg ttagagtggg gagcacgatt ttcggagctc gtcctccaaa aggagaagca aaagtggttc ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for CoimA.00860.a.A1.PW30517

PURIFICATION DATe:	12/20/2010
CONCENTRATION:	26.24mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	92

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCTCTCCG TCCCGCGCCT CCGCCTTGCT TGCGAACCTC TCCTCCGTGA CCTCCCGCAT CTCCGCCGCC TCAGTCCCCG GCAGTTCTGT CCGCCTCGTC GCTGTCTCCA AACTCAAGCC CGCATCCGAC ATCCTCGCCC TCTACAACCC ACCCACATCC CACCTTCATT TCGGTGAAAA CTACCTGCAA GAGCTCCTCG AAAAATCCAA AATCCTGCCC CCGGAAATCA GATGGCACTT CATCGGCGGG CTACAGTCGA ACAAATGCGT CACACTAGCG AGGGATGTGA GAGGTCTCTG GGCCGTCGAG AGTGTAGACA CACAGAAGAA AGCGACCCTG CTAGACAAAG GGTGGGGCGA GAGACTAAAG AATCACTCCG CACAGCAGCA GCAACAACAA CAGGGCAGAG CGGAGCCGGA GCGATTGCGT GTTTTTGTCC AGGTGAACAC CTCCGGAGAA GAGAGTAAGT CTGGTGTTGA GCCATCACAG GCGGTGGAAT TGTGCCGGTT TATCAGGGAG CAGTGTCCAC GGTTGAAGCT AGAGGGCCTA ATGACTATCG GGGCCATTGC TCGGTCCAAG GCAACAACCC CGGAGACTCA GAATGAAGAT TTTGACTGTT TGAGAGAGAC AAGGGATAGA ATTTGCCAAG AATTGGGTTT GGAAGGGGAG GAGAAGCTGG AGTTGAGCAT GGGTATGAGT GAAGACTTTG AGGGTGCTAT TGCCATGGGG AGCAATGAGG TTAGAGTGGG GAGCACGATT TTCGGAGCTC GTCCTCCAAA AGGAGAAGCA AAAGTGGTTC CCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA