CoimA.00717.a
Putative triosephosphate isomerase (TIM) (EC=5.3.1.1) (Triose-phosphate isomerase)
| CENTER ID: | CoimA.00717.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00717.a.A1.GE28391 | Full length( CoimA.00717.a ) | 1 | 289 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00717.a.A1.PS00678 | Full length( CoimA.00717.a ) | 1 | 289 |
Structures
3S6D
DEPOSITED: 5/25/2011
DETERMINATION: XRay
CLONE: CoimA.00717.a.A1.GE28391
PROTEIN: CoimA.00717.a.A1.PS00678
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_07933 |
| OrthoMCL: | OG5_155475 |
| RefSeq: | XP_001240770.1 |
| UniProt: | P0CL22 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASALAGPRF PPLPKTLLII SLKMYFTPSR TIDYIQGLLE PRNDIIRQEN RSRLLLALIP DFLTIYPCSE AIKEFESNLA APQDADTPPP LLLGAQDCFW DSLGPYTGEI SPVCLRDMNV SIVELGHAER RAIFGETDQQ VARKAAAAAD QGLIPLVCIG EVSTLGPIVS EAIGRAVGEC EAQIRPVLEA LPRDAPVIFA YEPVWAIGKP QPARVDHVGA VVSGIRSVIE RIDRHRKGEV RILYGGSAGP GLWGPGGLGK EVDGMFLGRF AHDIEGVRKV VREVEESLR
NT Sequence
ATGGCATCCG CACTGGCAGG GCCTCGTTTT CCCCCACTGC CAAAAACGCT CCTCATCATC TCTTTGAAAA TGTACTTTAC TCCCTCTCGA ACAATCGACT ATATCCAAGG CCTCCTAGAG CCCCGGAACG ACATCATCCG GCAAGAGAAT AGATCCAGAC TCCTTCTCGC TCTTATTCCA GATTTCCTTA CCATATATCC CTGCTCTGAA GCAATTAAAG AATTTGAATC CAACCTTGCC GCTCCGCAAG ATGCAGACAC ACCACCCCCA CTTCTCCTCG GTGCGCAGGA TTGTTTCTGG GACAGCTTGG GTCCTTATAC AGGCGAGATA TCCCCCGTCT GCTTGCGAGA CATGAATGTT TCAATTGTCG AGCTTGGACA TGCAGAGCGT CGCGCCATAT TCGGCGAGAC AGACCAGCAA GTTGCACGAA AGGCCGCCGC GGCAGCGGAC CAGGGTCTGA TCCCGCTGGT GTGTATTGGG GAAGTTTCCA CACTGGGACC AATCGTGTCA GAAGCAATTG GTCGCGCGGT GGGGGAGTGC GAGGCGCAAA TTAGACCGGT GCTGGAGGCG CTGCCGCGTG ATGCGCCGGT GATTTTTGCC TACGAGCCAG TGTGGGCCAT TGGGAAGCCA CAGCCCGCCA GGGTTGACCA TGTTGGTGCC GTTGTGTCGG GAATAAGGAG TGTTATTGAG AGAATTGATA GACATAGGAA AGGGGAAGTT AGGATCTTGT ATGGCGGGAG CGCGGGACCG GGACTATGGG GGCCAGGGGG GTTGGGAAAG GAGGTGGACG GGATGTTTCT GGGCAGGTTT GCACATGATA TTGAGGGTGT AAGGAAGGTG GTTAGGGAGG TGGAAGAGAG TTTGAGA
Details for CoimA.00717.a.A1.GE28391
| HARVESTED ON: | 2/2/2010 |
| SEQUENCED ON: | 2/3/2015 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASALAGPR FPPLPKTLLI ISLKMYFTPS RTIDYIQGLL EPRNDIIRQE NRSRLLLALI PDFLTIYPCS EAIKEFESNL AAPQDADTPP PLLLGAQDCF WDSLGPYTGE ISPVCLRDMN VSIVELGHAE RRAIFGETDQ QVARKAAAAA DQGLIPLVCI GEVSTLGPIV SEAIGRAVGE CEAQIRPVLE ALPRDAPVIF AYEPVWAIGK PQPARVDHVG AVVSGIRSVI ERIDRHRKGE VRILYGGSAG PGLWGPGGLG KEVDGMFLGR FAHDIEGVRK VVREVEESLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcat ccgcactggc agggcctcgt tttcccccac tgccaaaaac gctcctcatc atctctttga aaatgtactt tactccctct cgaacaatcg actatatcca aggcctccta gagccccgga acgacatcat ccggcaagag aatagatcca gactccttct cgctcttatt ccagatttcc ttaccatata tccctgctct gaagcaatta aagaatttga atccaacctt gccgctccgc aagatgcaga cacaccaccc ccacttctcc tcggtgcgca ggattgtttc tgggacagct tgggtcctta tacaggcgag atatcccccg tctgcttgcg agacatgaat gtttcaattg tcgagcttgg acatgcagag cgtcgcgcca tattcggcga gacagaccag caagttgcac gaaaggccgc cgcggcagcg gaccagggtc tgatcccgct ggtgtgtatt ggggaagttt ccacactggg accaatcgtg tcagaagcaa ttggtcgcgc ggtgggggag tgcgaggcgc aaattagacc ggtgctggag gcgctgccgc gtgatgcgcc ggtgattttt gcctacgagc cagtgtgggc cattgggaag ccacagcccg ccagggttga ccatgttggt gccgttgtgt cgggaataag gagtgttatt gagagaattg atagacatag gaaaggggaa gttaggatct tgtatggcgg gagcgcggga ccgggactat gggggccagg ggggttggga aaggaggtgg acgggatgtt tctgggcagg tttgcacatg atattgaggg tgtaaggaag gtggttaggg aggtggaaga gagtttgaga taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASALAGPR FPPLPKTLLI ISLKMYFTPS RTIDYIQGLL EPRNDIIRQE NRSRLLLALI PDFLTIYPCS EAIKEFESNL AAPQDADTPP PLLLGAQDCF WDSLGPYTGE ISPVCLRDMN VSIVELGHAE RRAIFGETDQ QVARKAAAAA DQGLIPLVCI GEVSTLGPIV SEAIGRAVGE CEAQIRPVLE ALPRDAPVIF AYEPVWAIGK PQPARVDHVG AVVSGIRSVI ERIDRHRKGE VRILYGGSAG PGLWGPGGLG KEVDGMFLGR FAHDIEGVRK VVREVEESLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatccgca ctggcagggc ctcgttttcc cccactgcca aaaacgctcc tcatcatctc tttgaaaatg tactttactc cctctcgaac aatcgactat atccaaggcc tcctagagcc ccggaacgac atcatccggc aagagaatag atccagactc cttctcgctc ttattccaga tttccttacc atatatccct gctctgaagc aattaaagaa tttgaatcca accttgccgc tccgcaagat gcagacacac cacccccact tctcctcggt gcgcaggatt gtttctggga cagcttgggt ccttatacag gcgagatatc ccccgtctgc ttgcgagaca tgaatgtttc aattgtcgag cttggacatg cagagcgtcg cgccatattc ggcgagacag accagcaagt tgcacgaaag gccgccgcgg cagcggacca gggtctgatc ccgctggtgt gtattgggga agtttccaca ctgggaccaa tcgtgtcaga agcaattggt cgcgcggtgg gggagtgcga ggcgcaaatt agaccggtgc tggaggcgct gccgcgtgat gcgccggtga tttttgccta cgagccagtg tgggccattg ggaagccaca gcccgccagg gttgaccatg ttggtgccgt tgtgtcggga ataaggagtg ttattgagag aattgataga cataggaaag gggaagttag gatcttgtat ggcgggagcg cgggaccggg actatggggg ccaggggggt tgggaaagga ggtggacggg atgtttctgg gcaggtttgc acatgatatt gagggtgtaa ggaaggtggt tagggaggtg gaagagagtt tgagaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for CoimA.00717.a.A1.PS00678
| PURIFICATION DATe: | 7/13/2010 |
| CONCENTRATION: | 53.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASALAGPR FPPLPKTLLI ISLKMYFTPS RTIDYIQGLL EPRNDIIRQE NRSRLLLALI PDFLTIYPCS EAIKEFESNL AAPQDADTPP PLLLGAQDCF WDSLGPYTGE ISPVCLRDMN VSIVELGHAE RRAIFGETDQ QVARKAAAAA DQGLIPLVCI GEVSTLGPIV SEAIGRAVGE CEAQIRPVLE ALPRDAPVIF AYEPVWAIGK PQPARVDHVG AVVSGIRSVI ERIDRHRKGE VRILYGGSAG PGLWGPGGLG KEVDGMFLGR FAHDIEGVRK VVREVEESLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcat ccgcactggc agggcctcgt tttcccccac tgccaaaaac gctcctcatc atctctttga aaatgtactt tactccctct cgaacaatcg actatatcca aggcctccta gagccccgga acgacatcat ccggcaagag aatagatcca gactccttct cgctcttatt ccagatttcc ttaccatata tccctgctct gaagcaatta aagaatttga atccaacctt gccgctccgc aagatgcaga cacaccaccc ccacttctcc tcggtgcgca ggattgtttc tgggacagct tgggtcctta tacaggcgag atatcccccg tctgcttgcg agacatgaat gtttcaattg tcgagcttgg acatgcagag cgtcgcgcca tattcggcga gacagaccag caagttgcac gaaaggccgc cgcggcagcg gaccagggtc tgatcccgct ggtgtgtatt ggggaagttt ccacactggg accaatcgtg tcagaagcaa ttggtcgcgc ggtgggggag tgcgaggcgc aaattagacc ggtgctggag gcgctgccgc gtgatgcgcc ggtgattttt gcctacgagc cagtgtgggc cattgggaag ccacagcccg ccagggttga ccatgttggt gccgttgtgt cgggaataag gagtgttatt gagagaattg atagacatag gaaaggggaa gttaggatct tgtatggcgg gagcgcggga ccgggactat gggggccagg ggggttggga aaggaggtgg acgggatgtt tctgggcagg tttgcacatg atattgaggg tgtaaggaag gtggttaggg aggtggaaga gagtttgaga taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASALAGPR FPPLPKTLLI ISLKMYFTPS RTIDYIQGLL EPRNDIIRQE NRSRLLLALI PDFLTIYPCS EAIKEFESNL AAPQDADTPP PLLLGAQDCF WDSLGPYTGE ISPVCLRDMN VSIVELGHAE RRAIFGETDQ QVARKAAAAA DQGLIPLVCI GEVSTLGPIV SEAIGRAVGE CEAQIRPVLE ALPRDAPVIF AYEPVWAIGK PQPARVDHVG AVVSGIRSVI ERIDRHRKGE VRILYGGSAG PGLWGPGGLG KEVDGMFLGR FAHDIEGVRK VVREVEESLR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATCCGCA CTGGCAGGGC CTCGTTTTCC CCCACTGCCA AAAACGCTCC TCATCATCTC TTTGAAAATG TACTTTACTC CCTCTCGAAC AATCGACTAT ATCCAAGGCC TCCTAGAGCC CCGGAACGAC ATCATCCGGC AAGAGAATAG ATCCAGACTC CTTCTCGCTC TTATTCCAGA TTTCCTTACC ATATATCCCT GCTCTGAAGC AATTAAAGAA TTTGAATCCA ACCTTGCCGC TCCGCAAGAT GCAGACACAC CACCCCCACT TCTCCTCGGT GCGCAGGATT GTTTCTGGGA CAGCTTGGGT CCTTATACAG GCGAGATATC CCCCGTCTGC TTGCGAGACA TGAATGTTTC AATTGTCGAG CTTGGACATG CAGAGCGTCG CGCCATATTC GGCGAGACAG ACCAGCAAGT TGCACGAAAG GCCGCCGCGG CAGCGGACCA GGGTCTGATC CCGCTGGTGT GTATTGGGGA AGTTTCCACA CTGGGACCAA TCGTGTCAGA AGCAATTGGT CGCGCGGTGG GGGAGTGCGA GGCGCAAATT AGACCGGTGC TGGAGGCGCT GCCGCGTGAT GCGCCGGTGA TTTTTGCCTA CGAGCCAGTG TGGGCCATTG GGAAGCCACA GCCCGCCAGG GTTGACCATG TTGGTGCCGT TGTGTCGGGA ATAAGGAGTG TTATTGAGAG AATTGATAGA CATAGGAAAG GGGAAGTTAG GATCTTGTAT GGCGGGAGCG CGGGACCGGG ACTATGGGGG CCAGGGGGGT TGGGAAAGGA GGTGGACGGG ATGTTTCTGG GCAGGTTTGC ACATGATATT GAGGGTGTAA GGAAGGTGGT TAGGGAGGTG GAAGAGAGTT TGAGAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA