CoimA.00629.a
Acetyl-coenzyme A synthetase (EC 6.2.1.1)
CENTER ID:
CoimA.00629.a
ORGANISM:
Coccidioides immitis RS
ASSOCIATED DISEASE:
California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
II
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
CoimA.00629.a.FS11.GE00456
full length
info
2
692
CoimA.00629.a.FS11.GE00456
CoimA.00629.a.HA11.GE00476
full length
info
39
666
CoimA.00629.a.HA11.GE00476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
CoimA.00629.a.FS11.PD00400
full length
info
2
692
PD00400
CoimA.00629.a.FS11.PD00401
full length
info
2
692
PD00401
CoimA.00629.a.HA11.PD00405
full length
info
39
666
PD00405
Structures
7KQZ
DEPOSITED: 11/18/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7KVY
DEPOSITED: 11/29/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7KQ6
DEPOSITED: 11/13/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7L3Q
DEPOSITED: 12/18/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7L3P
DEPOSITED: 12/18/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSETPAEPKL PVVVEAHQVD TFDVPGVFYE NHPHEPHLSG MNEYNQLYQQ SINDPDTFWA RMARDLITFE KDFDKTHIGT LEGGDNAWFV GGRLNASFNC VDRHAMRDPN KVAIIYEADE PGHGRSITYA ELLKEVSRLA WVMKSQGVRK GDTVAIYLPM IPEAIFALLA CARIGAIHSV VFAGFSSDSL RDRTLDARSK FIITTDEGKR GGKVIGTKKI VDEALKQCPD VTNCLVFKRT GADVPWTKGR DLWWHEEVDK YPNYLPAESM DSEDPLFLLY TSGSTGKPKG VMHTTAGYLV GAAATGKYVF DIHPADRFFC GGDVGWITGH TYVVYAPLLL GCTTVVFEST PAYPNFSRYW DVIEKHKVTQ FYVAPTALRL LKRAGDHHIN HEMKDLRILG SVGEPIAAEV WKWYHEVVGK RQAHIVDTYW QTETGSHVIT PLGGITPTKP GSASLPFFGI DPVILDPVTG AEIPGNDVEG ILAFRKPWPS MARTVWGDHK RYMDTYLNVY KGFYFTGDGA GRDHEGYYWI RGRVDDVVNV SGHRLSTAEI EAALIEHHCV AEAAVVGVPD PLTGQAVHAF VALKSGNDNR EQLQKELIMQ VRKSIGPFAA PKVVFVIDDL PKTRSGKIMR RILRKILSGE EDSLGDISTL SDPSVVNKII DTFHEWKKAM AAAAAAAAAV SATAPPNSTT G
NT Sequence
atgtctgaga cgcctgctga accaaagctc ccagtggtcg tggaggccca ccaggtcgat accttcgacg ttccaggagt attctatgag aaccaccccc atgaaccaca cctgagtggc atgaatgaat ataaccagct ctaccaacaa tctatcaatg atcctgacac cttctgggcg cgcatggcgc gcgatcttat tactttcgaa aaggatttcg acaagactca cattgggacc ctcgaaggcg gggataatgc ctggtttgtc ggtggtcgat taaatgcgtc cttcaactgc gttgatcgcc atgcaatgcg tgacccgaac aaggttgcaa tcatctatga agccgatgag cctggccacg gccgcagtat tacctatgca gaattgttga aggaagtctc cagactggcc tgggtgatga agagccaagg tgttaggaaa ggcgataccg tcgcgattta tcttcctatg atccctgagg ccatcttcgc gcttcttgcc tgtgcgcgta ttggcgccat tcattctgtg gtctttgcgg gcttctcttc cgattccctg cgtgatcgta ccctggatgc ccggtcaaaa ttcattatca caaccgatga gggaaagcgt ggtggaaaag tcattgggac taagaaaatt gttgatgaag ctttaaaaca atgcccagat gttaccaatt gccttgtttt caagagaact ggggccgacg tgccttggac caaaggccgt gacctgtggt ggcatgaaga ggttgataaa tatccaaatt atctgcctgc tgagtcaatg gactctgaag acccgctttt ccttctctac acatctggct ccaccgggaa accaaagggt gttatgcaca caactgctgg ctaccttgtt ggagcagctg caactggaaa atatgtcttt gatattcacc cagctgaccg ttttttttgc ggtggtgatg ttggatggat tactggacat acttatgttg tctatgctcc attgcttttg gggtgcacta ctgtggtgtt tgaaagcaca ccagcgtatc ccaatttctc gcgatactgg gatgttattg agaagcacaa ggtcacccag ttttatgtag cacctacggc tttgcgcctg ttgaaacgtg caggtgacca ccacataaat catgaaatga aggatttacg tattctgggc tctgttggtg aaccaatcgc tgcagaggtt tggaagtggt accatgaggt tgttgggaaa agacaagccc atattgttga tacatactgg caaaccgaga ccggctccca tgttatcacc cccttgggtg gtattactcc aactaaacca ggaagtgctt cgcttccatt ctttggtatt gaccctgtca ttctggatcc agttacaggt gcagagattc cgggaaatga tgtggaggga atccttgctt tcagaaagcc atggccaagc atggctcgca ctgtatgggg cgatcataaa cgatacatgg acacctatct caatgtttat aaaggctttt atttcactgg tgatggcgct ggacgtgatc atgaaggcta ttactggatt agaggtcgtg tggatgatgt tgtgaacgtc tctggccacc gcctttcaac tgcagagatc gaagcagcac ttatcgaaca ccactgtgtt gcagaagccg cggttgttgg agttcctgat ccgctaactg gacaagctgt gcatgcattt gtggccctga agagtggaaa tgacaataga gaacagctcc aaaaggagtt gattatgcaa gtccgcaaat cgattggacc atttgctgcc ccgaaggttg tgtttgtgat cgatgacctt ccgaaaacac gtagcgggaa gatcatgaga cgcatcttgc gaaagattct gagcggtgag gaggacagtc ttggtgacat ttcaacgctc tcggatccta gcgtcgtgaa taaaatcatc gacactttcc acgaatggaa aaaagctatg gcagctgccg ccgccgctgc tgctgctgtg tcagcaaccg cgccaccaaa cagcacaacc ggttaa
Details for CoimA.00629.a.FS11.GE00456
HARVESTED ON:
8/7/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
78kDa
OBSERVED MW:
78kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
Data not available
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CoimA.00629.a.HA11.GE00476
HARVESTED ON:
8/5/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
72kDa
OBSERVED MW:
72kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CoimA.00629.a.FS11.PD00400
PURIFICATION DATe:
10/2/2015
CONCENTRATION:
19.99mg/ml
OBSERVED MW:
78kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
Data not available
VIAL COUNT (approx.):
26
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CoimA.00629.a.FS11.PD00401
PURIFICATION DATe:
10/2/2015
CONCENTRATION:
20mg/ml
OBSERVED MW:
78kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
Data not available
VIAL COUNT (approx.):
33
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CoimA.00629.a.HA11.PD00405
PURIFICATION DATe:
10/13/2015
CONCENTRATION:
10.325mg/ml
OBSERVED MW:
72kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
10 mM HEPES pH 8.5, 20 mM NaCl
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
3
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable