CoimA.00629.a
Acetyl-coenzyme A synthetase (EC 6.2.1.1)
| CENTER ID: | CoimA.00629.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00629.a.FS11.GE00456 | full length | 2 | 692 | ||
| CoimA.00629.a.HA11.GE00476 | full length | 39 | 666 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00629.a.FS11.PD00400 | full length | 2 | 692 | ||
| CoimA.00629.a.FS11.PD00401 | full length | 2 | 692 | ||
| CoimA.00629.a.HA11.PD00405 | full length | 39 | 666 |
Structures
7KQZ
DEPOSITED: 11/18/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7KVY
DEPOSITED: 11/29/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7KQ6
DEPOSITED: 11/13/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7L3Q
DEPOSITED: 12/18/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
7L3P
DEPOSITED: 12/18/2020
DETERMINATION: XRay
CLONE: CoimA.00629.a.FS11.GE00456
PROTEIN: CoimA.00629.a.FS11.PD00401
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_01642 |
| RefSeq: | XP_001247871.2 |
| UniProt: | J3KJM0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSETPAEPKL PVVVEAHQVD TFDVPGVFYE NHPHEPHLSG MNEYNQLYQQ SINDPDTFWA RMARDLITFE KDFDKTHIGT LEGGDNAWFV GGRLNASFNC VDRHAMRDPN KVAIIYEADE PGHGRSITYA ELLKEVSRLA WVMKSQGVRK GDTVAIYLPM IPEAIFALLA CARIGAIHSV VFAGFSSDSL RDRTLDARSK FIITTDEGKR GGKVIGTKKI VDEALKQCPD VTNCLVFKRT GADVPWTKGR DLWWHEEVDK YPNYLPAESM DSEDPLFLLY TSGSTGKPKG VMHTTAGYLV GAAATGKYVF DIHPADRFFC GGDVGWITGH TYVVYAPLLL GCTTVVFEST PAYPNFSRYW DVIEKHKVTQ FYVAPTALRL LKRAGDHHIN HEMKDLRILG SVGEPIAAEV WKWYHEVVGK RQAHIVDTYW QTETGSHVIT PLGGITPTKP GSASLPFFGI DPVILDPVTG AEIPGNDVEG ILAFRKPWPS MARTVWGDHK RYMDTYLNVY KGFYFTGDGA GRDHEGYYWI RGRVDDVVNV SGHRLSTAEI EAALIEHHCV AEAAVVGVPD PLTGQAVHAF VALKSGNDNR EQLQKELIMQ VRKSIGPFAA PKVVFVIDDL PKTRSGKIMR RILRKILSGE EDSLGDISTL SDPSVVNKII DTFHEWKKAM AAAAAAAAAV SATAPPNSTT G
NT Sequence
atgtctgaga cgcctgctga accaaagctc ccagtggtcg tggaggccca ccaggtcgat accttcgacg ttccaggagt attctatgag aaccaccccc atgaaccaca cctgagtggc atgaatgaat ataaccagct ctaccaacaa tctatcaatg atcctgacac cttctgggcg cgcatggcgc gcgatcttat tactttcgaa aaggatttcg acaagactca cattgggacc ctcgaaggcg gggataatgc ctggtttgtc ggtggtcgat taaatgcgtc cttcaactgc gttgatcgcc atgcaatgcg tgacccgaac aaggttgcaa tcatctatga agccgatgag cctggccacg gccgcagtat tacctatgca gaattgttga aggaagtctc cagactggcc tgggtgatga agagccaagg tgttaggaaa ggcgataccg tcgcgattta tcttcctatg atccctgagg ccatcttcgc gcttcttgcc tgtgcgcgta ttggcgccat tcattctgtg gtctttgcgg gcttctcttc cgattccctg cgtgatcgta ccctggatgc ccggtcaaaa ttcattatca caaccgatga gggaaagcgt ggtggaaaag tcattgggac taagaaaatt gttgatgaag ctttaaaaca atgcccagat gttaccaatt gccttgtttt caagagaact ggggccgacg tgccttggac caaaggccgt gacctgtggt ggcatgaaga ggttgataaa tatccaaatt atctgcctgc tgagtcaatg gactctgaag acccgctttt ccttctctac acatctggct ccaccgggaa accaaagggt gttatgcaca caactgctgg ctaccttgtt ggagcagctg caactggaaa atatgtcttt gatattcacc cagctgaccg ttttttttgc ggtggtgatg ttggatggat tactggacat acttatgttg tctatgctcc attgcttttg gggtgcacta ctgtggtgtt tgaaagcaca ccagcgtatc ccaatttctc gcgatactgg gatgttattg agaagcacaa ggtcacccag ttttatgtag cacctacggc tttgcgcctg ttgaaacgtg caggtgacca ccacataaat catgaaatga aggatttacg tattctgggc tctgttggtg aaccaatcgc tgcagaggtt tggaagtggt accatgaggt tgttgggaaa agacaagccc atattgttga tacatactgg caaaccgaga ccggctccca tgttatcacc cccttgggtg gtattactcc aactaaacca ggaagtgctt cgcttccatt ctttggtatt gaccctgtca ttctggatcc agttacaggt gcagagattc cgggaaatga tgtggaggga atccttgctt tcagaaagcc atggccaagc atggctcgca ctgtatgggg cgatcataaa cgatacatgg acacctatct caatgtttat aaaggctttt atttcactgg tgatggcgct ggacgtgatc atgaaggcta ttactggatt agaggtcgtg tggatgatgt tgtgaacgtc tctggccacc gcctttcaac tgcagagatc gaagcagcac ttatcgaaca ccactgtgtt gcagaagccg cggttgttgg agttcctgat ccgctaactg gacaagctgt gcatgcattt gtggccctga agagtggaaa tgacaataga gaacagctcc aaaaggagtt gattatgcaa gtccgcaaat cgattggacc atttgctgcc ccgaaggttg tgtttgtgat cgatgacctt ccgaaaacac gtagcgggaa gatcatgaga cgcatcttgc gaaagattct gagcggtgag gaggacagtc ttggtgacat ttcaacgctc tcggatccta gcgtcgtgaa taaaatcatc gacactttcc acgaatggaa aaaagctatg gcagctgccg ccgccgctgc tgctgctgtg tcagcaaccg cgccaccaaa cagcacaacc ggttaa
Details for CoimA.00629.a.FS11.GE00456
| HARVESTED ON: | 8/7/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 78kDa |
| OBSERVED MW: | 78kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | Data not available |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGSETP AEPKLPVVVE AHQVDTFDVP GVFYENHPHE PHLSGMNEYN QLYQQSINDP DTFWARMARD LITFEKDFDK THIGTLEGGD NAWFVGGRLN ASFNCVDRHA MRDPNKVAII YEADEPGHGR SITYAELLKE VSRLAWVMKS QGVRKGDTVA IYLPMIPEAI FALLACARIG AIHSVVFAGF SSDSLRDRTL DARSKFIITT DEGKRGGKVI GTKKIVDEAL KQCPDVTNCL VFKRTGADVP WTKGRDLWWH EEVDKYPNYL PAESMDSEDP LFLLYTSGST GKPKGVMHTT AGYLVGAAAT GKYVFDIHPA DRFFCGGDVG WITGHTYVVY APLLLGCTTV VFESTPAYPN FSRYWDVIEK HKVTQFYVAP TALRLLKRAG DHHINHEMKD LRILGSVGEP IAAEVWKWYH EVVGKRQAHI VDTYWQTETG SHVITPLGGI TPTKPGSASL PFFGIDPVIL DPVTGAEIPG NDVEGILAFR KPWPSMARTV WGDHKRYMDT YLNVYKGFYF TGDGAGRDHE GYYWIRGRVD DVVNVSGHRL STAEIEAALI EHHCVAEAAV VGVPDPLTGQ AVHAFVALKS GNDNREQLQK ELIMQVRKSI GPFAAPKVVF VIDDLPKTRS GKIMRRILRK ILSGEEDSLG DISTLSDPSV VNKIIDTFHE WKKAMAAAAA AAAAVSATAP PNSTTG
Full NT Sequence (Expression Vector + Insert)
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aacttttaga gaccaaggag gtaaaaaatg caccatcacc accaccatca tcacgagaat ctgtacttcc aaggctccga aaccccggca gagccgaaac tgccggttgt cgttgaagcg caccaagtgg atactttcga tgtgccgggt gtgttctacg agaaccaccc gcatgagccg cacctgagcg gcatgaatga gtataaccaa ctgtaccagc agagcattaa cgacccggac acgttttggg cacgtatggc gcgtgacctg attacgttcg agaaagactt tgataaaacg cacattggta cgctggaggg tggtgacaat gcgtggtttg tgggcggccg tctgaatgcg agcttcaact gtgtggaccg tcacgccatg cgtgacccga acaaagttgc gatcatttat gaagcggatg agccgggtca tggtcgtagc attacctacg ccgaacttct gaaagaggtt agccgtctgg cttgggtcat gaaatcccag ggcgtacgca agggcgatac cgttgccatc tatctgccga tgatcccgga agccatcttc gcactgctgg cgtgtgcgcg tattggcgcg attcactctg tagtgtttgc gggtttcagc agcgattctc tgcgtgaccg tactctcgat gcgcgttcca aattcatcat caccaccgat gaaggtaagc gtggtggcaa ggttattggt accaagaaaa ttgtggacga agcgctgaaa cagtgccctg atgtcaccaa ttgcctggtc tttaaacgca ccggtgcaga tgttccgtgg accaagggtc gcgatctgtg gtggcacgaa gaggttgata agtacccgaa ttatctgcca gccgagagca tggacagcga agatcctctg tttctgctgt ataccagcgg cagcaccggc aaaccgaagg gtgtgatgca tactaccgca ggctatctgg tcggtgcagc ggcgaccggt aaatacgtgt tcgacatcca ccctgcggac cgctttttct gtggtggcga tgtgggctgg attacgggtc acacgtacgt cgtttatgcg ccgttgttgc tgggttgcac cacggtcgtg tttgagtcca cgccggcata cccgaatttc agccgttatt gggacgttat tgagaagcat aaagtgacgc agttttacgt tgctccgacc gccttacgtt tgctgaagcg cgcgggcgac catcacatta accacgagat gaaagacttg cgtatcttgg gcagcgtcgg cgagccgatc gccgcagaag tgtggaaatg gtatcatgaa gttgtgggta agcgtcaagc tcacatcgtc gatacctatt ggcagactga aaccggcagc catgtgatta cgccgctggg cggcatcacc ccgaccaagc caggtagcgc gtcgctgccg ttcttcggta tcgacccggt gatcctggac ccagtcactg gtgcagagat cccgggtaac gacgtcgagg gtatcctggc ctttcgcaag ccgtggccga gcatggcgcg cacggtctgg ggtgatcaca agcgctacat ggacacctac ctgaacgtct acaagggttt ctactttacc ggtgacggtg ccggtcgcga ccacgagggc tactattgga ttcgcggtcg cgttgatgat gtcgttaatg ttagcggtca tcgcctgagc accgcggaaa ttgaagcggc gctgatcgag catcactgcg tcgcagaggc cgcagttgtt ggcgtgccgg acccactgac gggccaagcc gtccatgctt ttgttgcgct gaagtctggc aatgataacc gtgaacagct gcagaaagaa ttgatcatgc aagttcgtaa aagcattggc ccgttcgcag cgccgaaagt cgtgtttgtg atcgatgact tgccgaaaac gcgtagcggt aagatcatgc gtcgtattct gcgcaagatc ctgagcggtg aggaagatag cctgggtgac atctctaccc tgagcgaccc gagcgttgtg aacaagatta ttgacacgtt ccacgagtgg aagaaagcaa tggcagcggc agcggctgca gctgcggctg tcagcgctac ggcgcctccg aacagcacca ccggctgata aggttgaggt ctcaccccct agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgcccc tgagacgcgt caatcgagtt cgtacctaag ggcgacaccc cctaattagc ccgggcgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggagt ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaaca aggggtgtta tgagccatat tcaggtataa atgggctcgc gataatgttc agaattggtt aattggttgt aacactgacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagaatatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcca cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcgtt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcactcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgcctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagcgg cgcgccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatat gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgag gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag atagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgccagt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg a
Details for CoimA.00629.a.HA11.GE00476
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 72kDa |
| OBSERVED MW: | 72kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGSGMN EYNQLYQQSI NDPDTFWARM ARDLITFEKD FDKTHIGTLE GGDNAWFVGG RLNASFNCVD RHAMRDPNKV AIIYEADEPG HGRSITYAEL LKEVSRLAWV MKSQGVRKGD TVAIYLPMIP EAIFALLACA RIGAIHSVVF AGFSSDSLRD RTLDARSKFI ITTDEGKRGG KVIGTKKIVD EALKQCPDVT NCLVFKRTGA DVPWTKGRDL WWHEEVDKYP NYLPAESMDS EDPLFLLYTS GSTGKPKGVM HTTAGYLVGA AATGKYVFDI HPADRFFCGG DVGWITGHTY VVYAPLLLGC TTVVFESTPA YPNFSRYWDV IEKHKVTQFY VAPTALRLLK RAGDHHINHE MKDLRILGSV GEPIAAEVWK WYHEVVGKRQ AHIVDTYWQT ETGSHVITPL GGITPTKPGS ASLPFFGIDP VILDPVTGAE IPGNDVEGIL AFRKPWPSMA RTVWGDHKRY MDTYLNVYKG FYFTGDGAGR DHEGYYWIRG RVDDVVNVSG HRLSTAEIEA ALIEHHCVAE AAVVGVPDPL TGQAVHAFVA LKSGNDNREQ LQKELIMQVR KSIGPFAAPK VVFVIDDLPK TRSGKIMRRI LRKILSGEED SLGDISTLSD PSVVNKIIDT FHE
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgcaccatc accaccacca tcatcacgag aatctgtact tccaaggcag cggcatgaat gagtataacc aactgtacca gcagagcatt aacgacccgg acacgttttg ggcacgtatg gcgcgtgacc tgattacgtt cgagaaagac tttgataaaa cgcacattgg tacgctggag ggtggtgaca atgcgtggtt tgtgggcggc cgtctgaatg cgagcttcaa ctgtgtggac cgtcacgcca tgcgtgaccc gaacaaagtt gcgatcattt atgaagcgga tgagccgggt catggtcgta gcattaccta cgccgaactt ctgaaagagg ttagccgtct ggcttgggtc atgaaatccc agggcgtacg caagggcgat accgttgcca tctatctgcc gatgatcccg gaagccatct tcgcactgct ggcgtgtgcg cgtattggcg cgattcactc tgtagtgttt gcgggtttca gcagcgattc tctgcgtgac cgtactctcg atgcgcgttc caaattcatc atcaccaccg atgaaggtaa gcgtggtggc aaggttattg gtaccaagaa aattgtggac gaagcgctga aacagtgccc tgatgtcacc aattgcctgg tctttaaacg caccggtgca gatgttccgt ggaccaaggg tcgcgatctg tggtggcacg aagaggttga taagtacccg aattatctgc cagccgagag catggacagc gaagatcctc tgtttctgct gtataccagc ggcagcaccg gcaaaccgaa gggtgtgatg catactaccg caggctatct ggtcggtgca gcggcgaccg gtaaatacgt gttcgacatc caccctgcgg accgcttttt ctgtggtggc gatgtgggct ggattacggg tcacacgtac gtcgtttatg cgccgttgtt gctgggttgc accacggtcg tgtttgagtc cacgccggca tacccgaatt tcagccgtta ttgggacgtt attgagaagc ataaagtgac gcagttttac gttgctccga ccgccttacg tttgctgaag cgcgcgggcg accatcacat taaccacgag atgaaagact tgcgtatctt gggcagcgtc ggcgagccga tcgccgcaga agtgtggaaa tggtatcatg aagttgtggg taagcgtcaa gctcacatcg tcgataccta ttggcagact gaaaccggca gccatgtgat tacgccgctg ggcggcatca ccccgaccaa gccaggtagc gcgtcgctgc cgttcttcgg tatcgacccg gtgatcctgg acccagtcac tggtgcagag atcccgggta acgacgtcga gggtatcctg gcctttcgca agccgtggcc gagcatggcg cgcacggtct ggggtgatca caagcgctac atggacacct acctgaacgt ctacaagggt ttctacttta ccggtgacgg tgccggtcgc gaccacgagg gctactattg gattcgcggt cgcgttgatg atgtcgttaa tgttagcggt catcgcctga gcaccgcgga aattgaagcg gcgctgatcg agcatcactg cgtcgcagag gccgcagttg ttggcgtgcc ggacccactg acgggccaag ccgtccatgc ttttgttgcg ctgaagtctg gcaatgataa ccgtgaacag ctgcagaaag aattgatcat gcaagttcgt aaaagcattg gcccgttcgc agcgccgaaa gtcgtgtttg tgatcgatga cttgccgaaa acgcgtagcg gtaagatcat gcgtcgtatt ctgcgcaaga tcctgagcgg tgaggaagat agcctgggtg acatctctac cctgagcgac ccgagcgttg tgaacaagat tattgacacg ttccacgagt gataagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for CoimA.00629.a.FS11.PD00400
| PURIFICATION DATe: | 10/2/2015 |
| CONCENTRATION: | 19.99mg/ml |
| OBSERVED MW: | 78kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 26 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGSETP AEPKLPVVVE AHQVDTFDVP GVFYENHPHE PHLSGMNEYN QLYQQSINDP DTFWARMARD LITFEKDFDK THIGTLEGGD NAWFVGGRLN ASFNCVDRHA MRDPNKVAII YEADEPGHGR SITYAELLKE VSRLAWVMKS QGVRKGDTVA IYLPMIPEAI FALLACARIG AIHSVVFAGF SSDSLRDRTL DARSKFIITT DEGKRGGKVI GTKKIVDEAL KQCPDVTNCL VFKRTGADVP WTKGRDLWWH EEVDKYPNYL PAESMDSEDP LFLLYTSGST GKPKGVMHTT AGYLVGAAAT GKYVFDIHPA DRFFCGGDVG WITGHTYVVY APLLLGCTTV VFESTPAYPN FSRYWDVIEK HKVTQFYVAP TALRLLKRAG DHHINHEMKD LRILGSVGEP IAAEVWKWYH EVVGKRQAHI VDTYWQTETG SHVITPLGGI TPTKPGSASL PFFGIDPVIL DPVTGAEIPG NDVEGILAFR KPWPSMARTV WGDHKRYMDT YLNVYKGFYF TGDGAGRDHE GYYWIRGRVD DVVNVSGHRL STAEIEAALI EHHCVAEAAV VGVPDPLTGQ AVHAFVALKS GNDNREQLQK ELIMQVRKSI GPFAAPKVVF VIDDLPKTRS GKIMRRILRK ILSGEEDSLG DISTLSDPSV VNKIIDTFHE WKKAMAAAAA AAAAVSATAP PNSTTG
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CACCATCACC ACCACCATCA TCACGAGAAT CTGTACTTCC AAGGCTCCGA AACCCCGGCA GAGCCGAAAC TGCCGGTTGT CGTTGAAGCG CACCAAGTGG ATACTTTCGA TGTGCCGGGT GTGTTCTACG AGAACCACCC GCATGAGCCG CACCTGAGCG GCATGAATGA GTATAACCAA CTGTACCAGC AGAGCATTAA CGACCCGGAC ACGTTTTGGG CACGTATGGC GCGTGACCTG ATTACGTTCG AGAAAGACTT TGATAAAACG CACATTGGTA CGCTGGAGGG TGGTGACAAT GCGTGGTTTG TGGGCGGCCG TCTGAATGCG AGCTTCAACT GTGTGGACCG TCACGCCATG CGTGACCCGA ACAAAGTTGC GATCATTTAT GAAGCGGATG AGCCGGGTCA TGGTCGTAGC ATTACCTACG CCGAACTTCT GAAAGAGGTT AGCCGTCTGG CTTGGGTCAT GAAATCCCAG GGCGTACGCA AGGGCGATAC CGTTGCCATC TATCTGCCGA TGATCCCGGA AGCCATCTTC GCACTGCTGG CGTGTGCGCG TATTGGCGCG ATTCACTCTG TAGTGTTTGC GGGTTTCAGC AGCGATTCTC TGCGTGACCG TACTCTCGAT GCGCGTTCCA AATTCATCAT CACCACCGAT GAAGGTAAGC GTGGTGGCAA GGTTATTGGT ACCAAGAAAA TTGTGGACGA AGCGCTGAAA CAGTGCCCTG ATGTCACCAA TTGCCTGGTC TTTAAACGCA CCGGTGCAGA TGTTCCGTGG ACCAAGGGTC GCGATCTGTG GTGGCACGAA GAGGTTGATA AGTACCCGAA TTATCTGCCA GCCGAGAGCA TGGACAGCGA AGATCCTCTG TTTCTGCTGT ATACCAGCGG CAGCACCGGC AAACCGAAGG GTGTGATGCA TACTACCGCA GGCTATCTGG TCGGTGCAGC GGCGACCGGT AAATACGTGT TCGACATCCA CCCTGCGGAC CGCTTTTTCT GTGGTGGCGA TGTGGGCTGG ATTACGGGTC ACACGTACGT CGTTTATGCG CCGTTGTTGC TGGGTTGCAC CACGGTCGTG TTTGAGTCCA CGCCGGCATA CCCGAATTTC AGCCGTTATT GGGACGTTAT TGAGAAGCAT AAAGTGACGC AGTTTTACGT TGCTCCGACC GCCTTACGTT TGCTGAAGCG CGCGGGCGAC CATCACATTA ACCACGAGAT GAAAGACTTG CGTATCTTGG GCAGCGTCGG CGAGCCGATC GCCGCAGAAG TGTGGAAATG GTATCATGAA GTTGTGGGTA AGCGTCAAGC TCACATCGTC GATACCTATT GGCAGACTGA AACCGGCAGC CATGTGATTA CGCCGCTGGG CGGCATCACC CCGACCAAGC CAGGTAGCGC GTCGCTGCCG TTCTTCGGTA TCGACCCGGT GATCCTGGAC CCAGTCACTG GTGCAGAGAT CCCGGGTAAC GACGTCGAGG GTATCCTGGC CTTTCGCAAG CCGTGGCCGA GCATGGCGCG CACGGTCTGG GGTGATCACA AGCGCTACAT GGACACCTAC CTGAACGTCT ACAAGGGTTT CTACTTTACC GGTGACGGTG CCGGTCGCGA CCACGAGGGC TACTATTGGA TTCGCGGTCG CGTTGATGAT GTCGTTAATG TTAGCGGTCA TCGCCTGAGC ACCGCGGAAA TTGAAGCGGC GCTGATCGAG CATCACTGCG TCGCAGAGGC CGCAGTTGTT GGCGTGCCGG ACCCACTGAC GGGCCAAGCC GTCCATGCTT TTGTTGCGCT GAAGTCTGGC AATGATAACC GTGAACAGCT GCAGAAAGAA TTGATCATGC AAGTTCGTAA AAGCATTGGC CCGTTCGCAG CGCCGAAAGT CGTGTTTGTG ATCGATGACT TGCCGAAAAC GCGTAGCGGT AAGATCATGC GTCGTATTCT GCGCAAGATC CTGAGCGGTG AGGAAGATAG CCTGGGTGAC ATCTCTACCC TGAGCGACCC GAGCGTTGTG AACAAGATTA TTGACACGTT CCACGAGTGG AAGAAAGCAA TGGCAGCGGC AGCGGCTGCA GCTGCGGCTG TCAGCGCTAC GGCGCCTCCG AACAGCACCA CCGGCTGATA AGGTTGAGGT CTCACCCCCT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCCCC TGAGACGCGT CAATCGAGTT CGTACCTAAG GGCGACACCC CCTAATTAGC CCGGGCGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CATGGGGAGT CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAACA AGGGGTGTTA TGAGCCATAT TCAGGTATAA ATGGGCTCGC GATAATGTTC AGAATTGGTT AATTGGTTGT AACACTGACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAATATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCA CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCGTT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCACTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGCCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAAGCGG CGCGCCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATAT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAG GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ATAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCAGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG A
Details for CoimA.00629.a.FS11.PD00401
| PURIFICATION DATe: | 10/2/2015 |
| CONCENTRATION: | 20mg/ml |
| OBSERVED MW: | 78kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 33 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGSETP AEPKLPVVVE AHQVDTFDVP GVFYENHPHE PHLSGMNEYN QLYQQSINDP DTFWARMARD LITFEKDFDK THIGTLEGGD NAWFVGGRLN ASFNCVDRHA MRDPNKVAII YEADEPGHGR SITYAELLKE VSRLAWVMKS QGVRKGDTVA IYLPMIPEAI FALLACARIG AIHSVVFAGF SSDSLRDRTL DARSKFIITT DEGKRGGKVI GTKKIVDEAL KQCPDVTNCL VFKRTGADVP WTKGRDLWWH EEVDKYPNYL PAESMDSEDP LFLLYTSGST GKPKGVMHTT AGYLVGAAAT GKYVFDIHPA DRFFCGGDVG WITGHTYVVY APLLLGCTTV VFESTPAYPN FSRYWDVIEK HKVTQFYVAP TALRLLKRAG DHHINHEMKD LRILGSVGEP IAAEVWKWYH EVVGKRQAHI VDTYWQTETG SHVITPLGGI TPTKPGSASL PFFGIDPVIL DPVTGAEIPG NDVEGILAFR KPWPSMARTV WGDHKRYMDT YLNVYKGFYF TGDGAGRDHE GYYWIRGRVD DVVNVSGHRL STAEIEAALI EHHCVAEAAV VGVPDPLTGQ AVHAFVALKS GNDNREQLQK ELIMQVRKSI GPFAAPKVVF VIDDLPKTRS GKIMRRILRK ILSGEEDSLG DISTLSDPSV VNKIIDTFHE WKKAMAAAAA AAAAVSATAP PNSTTG
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CACCATCACC ACCACCATCA TCACGAGAAT CTGTACTTCC AAGGCTCCGA AACCCCGGCA GAGCCGAAAC TGCCGGTTGT CGTTGAAGCG CACCAAGTGG ATACTTTCGA TGTGCCGGGT GTGTTCTACG AGAACCACCC GCATGAGCCG CACCTGAGCG GCATGAATGA GTATAACCAA CTGTACCAGC AGAGCATTAA CGACCCGGAC ACGTTTTGGG CACGTATGGC GCGTGACCTG ATTACGTTCG AGAAAGACTT TGATAAAACG CACATTGGTA CGCTGGAGGG TGGTGACAAT GCGTGGTTTG TGGGCGGCCG TCTGAATGCG AGCTTCAACT GTGTGGACCG TCACGCCATG CGTGACCCGA ACAAAGTTGC GATCATTTAT GAAGCGGATG AGCCGGGTCA TGGTCGTAGC ATTACCTACG CCGAACTTCT GAAAGAGGTT AGCCGTCTGG CTTGGGTCAT GAAATCCCAG GGCGTACGCA AGGGCGATAC CGTTGCCATC TATCTGCCGA TGATCCCGGA AGCCATCTTC GCACTGCTGG CGTGTGCGCG TATTGGCGCG ATTCACTCTG TAGTGTTTGC GGGTTTCAGC AGCGATTCTC TGCGTGACCG TACTCTCGAT GCGCGTTCCA AATTCATCAT CACCACCGAT GAAGGTAAGC GTGGTGGCAA GGTTATTGGT ACCAAGAAAA TTGTGGACGA AGCGCTGAAA CAGTGCCCTG ATGTCACCAA TTGCCTGGTC TTTAAACGCA CCGGTGCAGA TGTTCCGTGG ACCAAGGGTC GCGATCTGTG GTGGCACGAA GAGGTTGATA AGTACCCGAA TTATCTGCCA GCCGAGAGCA TGGACAGCGA AGATCCTCTG TTTCTGCTGT ATACCAGCGG CAGCACCGGC AAACCGAAGG GTGTGATGCA TACTACCGCA GGCTATCTGG TCGGTGCAGC GGCGACCGGT AAATACGTGT TCGACATCCA CCCTGCGGAC CGCTTTTTCT GTGGTGGCGA TGTGGGCTGG ATTACGGGTC ACACGTACGT CGTTTATGCG CCGTTGTTGC TGGGTTGCAC CACGGTCGTG TTTGAGTCCA CGCCGGCATA CCCGAATTTC AGCCGTTATT GGGACGTTAT TGAGAAGCAT AAAGTGACGC AGTTTTACGT TGCTCCGACC GCCTTACGTT TGCTGAAGCG CGCGGGCGAC CATCACATTA ACCACGAGAT GAAAGACTTG CGTATCTTGG GCAGCGTCGG CGAGCCGATC GCCGCAGAAG TGTGGAAATG GTATCATGAA GTTGTGGGTA AGCGTCAAGC TCACATCGTC GATACCTATT GGCAGACTGA AACCGGCAGC CATGTGATTA CGCCGCTGGG CGGCATCACC CCGACCAAGC CAGGTAGCGC GTCGCTGCCG TTCTTCGGTA TCGACCCGGT GATCCTGGAC CCAGTCACTG GTGCAGAGAT CCCGGGTAAC GACGTCGAGG GTATCCTGGC CTTTCGCAAG CCGTGGCCGA GCATGGCGCG CACGGTCTGG GGTGATCACA AGCGCTACAT GGACACCTAC CTGAACGTCT ACAAGGGTTT CTACTTTACC GGTGACGGTG CCGGTCGCGA CCACGAGGGC TACTATTGGA TTCGCGGTCG CGTTGATGAT GTCGTTAATG TTAGCGGTCA TCGCCTGAGC ACCGCGGAAA TTGAAGCGGC GCTGATCGAG CATCACTGCG TCGCAGAGGC CGCAGTTGTT GGCGTGCCGG ACCCACTGAC GGGCCAAGCC GTCCATGCTT TTGTTGCGCT GAAGTCTGGC AATGATAACC GTGAACAGCT GCAGAAAGAA TTGATCATGC AAGTTCGTAA AAGCATTGGC CCGTTCGCAG CGCCGAAAGT CGTGTTTGTG ATCGATGACT TGCCGAAAAC GCGTAGCGGT AAGATCATGC GTCGTATTCT GCGCAAGATC CTGAGCGGTG AGGAAGATAG CCTGGGTGAC ATCTCTACCC TGAGCGACCC GAGCGTTGTG AACAAGATTA TTGACACGTT CCACGAGTGG AAGAAAGCAA TGGCAGCGGC AGCGGCTGCA GCTGCGGCTG TCAGCGCTAC GGCGCCTCCG AACAGCACCA CCGGCTGATA AGGTTGAGGT CTCACCCCCT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCCCC TGAGACGCGT CAATCGAGTT CGTACCTAAG GGCGACACCC CCTAATTAGC CCGGGCGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CATGGGGAGT CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAACA AGGGGTGTTA TGAGCCATAT TCAGGTATAA ATGGGCTCGC GATAATGTTC AGAATTGGTT AATTGGTTGT AACACTGACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAATATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCA CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCGTT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCACTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGCCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAAGCGG CGCGCCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATAT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAG GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ATAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCAGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG A
Details for CoimA.00629.a.HA11.PD00405
| PURIFICATION DATe: | 10/13/2015 |
| CONCENTRATION: | 10.325mg/ml |
| OBSERVED MW: | 72kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 10 mM HEPES pH 8.5, 20 mM NaCl |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGSGMN EYNQLYQQSI NDPDTFWARM ARDLITFEKD FDKTHIGTLE GGDNAWFVGG RLNASFNCVD RHAMRDPNKV AIIYEADEPG HGRSITYAEL LKEVSRLAWV MKSQGVRKGD TVAIYLPMIP EAIFALLACA RIGAIHSVVF AGFSSDSLRD RTLDARSKFI ITTDEGKRGG KVIGTKKIVD EALKQCPDVT NCLVFKRTGA DVPWTKGRDL WWHEEVDKYP NYLPAESMDS EDPLFLLYTS GSTGKPKGVM HTTAGYLVGA AATGKYVFDI HPADRFFCGG DVGWITGHTY VVYAPLLLGC TTVVFESTPA YPNFSRYWDV IEKHKVTQFY VAPTALRLLK RAGDHHINHE MKDLRILGSV GEPIAAEVWK WYHEVVGKRQ AHIVDTYWQT ETGSHVITPL GGITPTKPGS ASLPFFGIDP VILDPVTGAE IPGNDVEGIL AFRKPWPSMA RTVWGDHKRY MDTYLNVYKG FYFTGDGAGR DHEGYYWIRG RVDDVVNVSG HRLSTAEIEA ALIEHHCVAE AAVVGVPDPL TGQAVHAFVA LKSGNDNREQ LQKELIMQVR KSIGPFAAPK VVFVIDDLPK TRSGKIMRRI LRKILSGEED SLGDISTLSD PSVVNKIIDT FHE
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGCACCATC ACCACCACCA TCATCACGAG AATCTGTACT TCCAAGGCAG CGGCATGAAT GAGTATAACC AACTGTACCA GCAGAGCATT AACGACCCGG ACACGTTTTG GGCACGTATG GCGCGTGACC TGATTACGTT CGAGAAAGAC TTTGATAAAA CGCACATTGG TACGCTGGAG GGTGGTGACA ATGCGTGGTT TGTGGGCGGC CGTCTGAATG CGAGCTTCAA CTGTGTGGAC CGTCACGCCA TGCGTGACCC GAACAAAGTT GCGATCATTT ATGAAGCGGA TGAGCCGGGT CATGGTCGTA GCATTACCTA CGCCGAACTT CTGAAAGAGG TTAGCCGTCT GGCTTGGGTC ATGAAATCCC AGGGCGTACG CAAGGGCGAT ACCGTTGCCA TCTATCTGCC GATGATCCCG GAAGCCATCT TCGCACTGCT GGCGTGTGCG CGTATTGGCG CGATTCACTC TGTAGTGTTT GCGGGTTTCA GCAGCGATTC TCTGCGTGAC CGTACTCTCG ATGCGCGTTC CAAATTCATC ATCACCACCG ATGAAGGTAA GCGTGGTGGC AAGGTTATTG GTACCAAGAA AATTGTGGAC GAAGCGCTGA AACAGTGCCC TGATGTCACC AATTGCCTGG TCTTTAAACG CACCGGTGCA GATGTTCCGT GGACCAAGGG TCGCGATCTG TGGTGGCACG AAGAGGTTGA TAAGTACCCG AATTATCTGC CAGCCGAGAG CATGGACAGC GAAGATCCTC TGTTTCTGCT GTATACCAGC GGCAGCACCG GCAAACCGAA GGGTGTGATG CATACTACCG CAGGCTATCT GGTCGGTGCA GCGGCGACCG GTAAATACGT GTTCGACATC CACCCTGCGG ACCGCTTTTT CTGTGGTGGC GATGTGGGCT GGATTACGGG TCACACGTAC GTCGTTTATG CGCCGTTGTT GCTGGGTTGC ACCACGGTCG TGTTTGAGTC CACGCCGGCA TACCCGAATT TCAGCCGTTA TTGGGACGTT ATTGAGAAGC ATAAAGTGAC GCAGTTTTAC GTTGCTCCGA CCGCCTTACG TTTGCTGAAG CGCGCGGGCG ACCATCACAT TAACCACGAG ATGAAAGACT TGCGTATCTT GGGCAGCGTC GGCGAGCCGA TCGCCGCAGA AGTGTGGAAA TGGTATCATG AAGTTGTGGG TAAGCGTCAA GCTCACATCG TCGATACCTA TTGGCAGACT GAAACCGGCA GCCATGTGAT TACGCCGCTG GGCGGCATCA CCCCGACCAA GCCAGGTAGC GCGTCGCTGC CGTTCTTCGG TATCGACCCG GTGATCCTGG ACCCAGTCAC TGGTGCAGAG ATCCCGGGTA ACGACGTCGA GGGTATCCTG GCCTTTCGCA AGCCGTGGCC GAGCATGGCG CGCACGGTCT GGGGTGATCA CAAGCGCTAC ATGGACACCT ACCTGAACGT CTACAAGGGT TTCTACTTTA CCGGTGACGG TGCCGGTCGC GACCACGAGG GCTACTATTG GATTCGCGGT CGCGTTGATG ATGTCGTTAA TGTTAGCGGT CATCGCCTGA GCACCGCGGA AATTGAAGCG GCGCTGATCG AGCATCACTG CGTCGCAGAG GCCGCAGTTG TTGGCGTGCC GGACCCACTG ACGGGCCAAG CCGTCCATGC TTTTGTTGCG CTGAAGTCTG GCAATGATAA CCGTGAACAG CTGCAGAAAG AATTGATCAT GCAAGTTCGT AAAAGCATTG GCCCGTTCGC AGCGCCGAAA GTCGTGTTTG TGATCGATGA CTTGCCGAAA ACGCGTAGCG GTAAGATCAT GCGTCGTATT CTGCGCAAGA TCCTGAGCGG TGAGGAAGAT AGCCTGGGTG ACATCTCTAC CCTGAGCGAC CCGAGCGTTG TGAACAAGAT TATTGACACG TTCCACGAGT GATAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T