CoimA.00559.a
Putative deoxyribose-phosphate aldolase
| CENTER ID: | CoimA.00559.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00559.a.A1.GE27319 | Full length( CoimA.00559.a ) | 1 | 267 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00559.a.A1.PS00460 | Full length( CoimA.00559.a ) | 1 | 267 |
Structures
3OA3
DEPOSITED: 8/4/2010
DETERMINATION: XRay
CLONE: CoimA.00559.a.A1.GE27319
PROTEIN: CoimA.00559.a.A1.PS00460
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_00461 |
| UniProt: | Q1EAF2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSLNNEEWD LLISGKKATL QYPIPLLCYP APEVVSIAQI IDHTQLSLSA TGSQIDVLCA EAKEYGFATV CVRPDYVSRA VQYLQGTQVG VTCVIGFHEG TYSTDQKVSE AKRAMQNGAS ELDMVMNYPW LSEKRYTDVF QDIRAVRLAA KDAILKVILE TSQLTADEII AGCVLSSLAG ADYVKTSTGF NGPGASIENV SLMSAVCDSL QSETRVKASG GIRTIEDCVK MVRAGAERLG ASAGVKIVNE TRLGNRQVDE PMEPTNY
NT Sequence
ATGTCGTCTC TCAACAACGA GGAATGGGAT TTGCTCATCT CAGGAAAGAA AGCTACACTT CAATATCCTA TTCCACTATT ATGCTACCCT GCCCCTGAGG TTGTTTCAAT TGCTCAGATA ATTGATCACA CCCAGCTCTC GCTCTCCGCC ACCGGGTCTC AAATTGACGT CTTGTGCGCT GAAGCGAAAG AATATGGTTT TGCAACCGTA TGTGTCAGGC CGGACTATGT CTCTCGGGCA GTTCAGTATC TTCAAGGAAC CCAAGTTGGA GTGACATGTG TGATTGGATT CCATGAAGGG ACGTATTCAA CCGACCAGAA AGTGAGCGAG GCGAAACGTG CCATGCAGAA TGGAGCCAGC GAATTGGATA TGGTGATGAA CTACCCTTGG CTCAGCGAAA AACGCTATAC CGACGTATTC CAGGATATTC GCGCGGTGAG ACTTGCTGCA AAGGATGCGA TTTTGAAGGT CATTCTCGAG ACATCCCAGT TGACCGCAGA TGAGATTATC GCCGGGTGCG TACTCTCGAG TCTCGCAGGT GCAGACTATG TGAAGACCAG TACGGGTTTT AACGGTCCTG GTGCGAGTAT TGAAAATGTT TCGTTGATGA GTGCTGTCTG TGACAGCTTA CAATCGGAGA CCAGGGTGAA AGCCAGTGGC GGCATAAGGA CAATCGAAGA TTGCGTGAAG ATGGTTAGGG CAGGAGCAGA GAGACTAGGC GCAAGCGCTG GTGTGAAGAT CGTGAATGAA ACTCGGCTGG GAAACCGTCA AGTGGATGAA CCCATGGAGC CAACAAACTA C
Details for CoimA.00559.a.A1.GE27319
| HARVESTED ON: | 10/23/2009 |
| SEQUENCED ON: | 10/29/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSLNNEEW DLLISGKKAT LQYPIPLLCY PAPEVVSIAQ IIDHTQLSLS ATGSQIDVLC AEAKEYGFAT VCVRPDYVSR AVQYLQGTQV GVTCVIGFHE GTYSTDQKVS EAKRAMQNGA SELDMVMNYP WLSEKRYTDV FQDIRAVRLA AKDAILKVIL ETSQLTADEI IAGCVLSSLA GADYVKTSTG FNGPGASIEN XSLMSAVCDS LQSETRVKAS GGIRTIEXCV KMVRAGAERL XASAGVKIVN ETRLGNRQVD EPM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgt ctctcaacaa cgaggaatgg gatttgctca tctcaggaaa gaaagctaca cttcaatatc ctattccact attatgctac cctgcccctg aggttgtttc aattgctcag ataattgatc acacccagct ctcgctctcc gccaccgggt ctcaaattga cgtcttgtgc gctgaagcga aagaatatgg ttttgcaacc gtatgtgtca ggccggacta tgtctctcgg gcagttcagt atcttcaagg aacccaagtt ggagtgacat gtgtgattgg attccatgaa gggacgtatt caaccgacca gaaagtgagc gaggcgaaac gtgccatgca gaatggagcc agcgaattgg atatggtgat gaactaccct tggctcagcg aaaaacgcta taccgacgta ttccaggata ttcgcgcggt gagacttgct gcaaaggatg cgattttgaa ggtcattctc gagacatccc agttgaccgc agatgagatt atcgccgggt gcgtactctc gagtctcgca ggtgcagact atgtgaagac cagtacgggt tttaacggtc ctggtgcgag tattgaaaat gnttcgttga tgagtgctgt ctgtgacagc ttacaatcgg agaccagggt gaaagccagt ggcggcataa ggacaatcga agnttgcgtg aagatggtta gggcaggagc agagagacta gncgcaagcg ctggtgtgaa gatcgtgaat gaaactcggc tgggaaaccg tcaagtggat gaacccatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSLNNEEW DLLISGKKAT LQYPIPLLCY PAPEVVSIAQ IIDHTQLSLS ATGSQIDVLC AEAKEYGFAT VCVRPDYVSR AVQYLQGTQV GVTCVIGFHE GTYSTDQKVS EAKRAMQNGA SELDMVMNYP WLSEKRYTDV FQDIRAVRLA AKDAILKVIL ETSQLTADEI IAGCVLSSLA GADYVKTSTG FNGPGASIEN VSLMSAVCDS LQSETRVKAS GGIRTIEDCV KMVRAGAERL GASAGVKIVN ETRLGNRQVD EPMEPTNY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtctctc aacaacgagg aatgggattt gctcatctca ggaaagaaag ctacacttca atatcctatt ccactattat gctaccctgc ccctgaggtt gtttcaattg ctcagataat tgatcacacc cagctctcgc tctccgccac cgggtctcaa attgacgtct tgtgcgctga agcgaaagaa tatggttttg caaccgtatg tgtcaggccg gactatgtct ctcgggcagt tcagtatctt caaggaaccc aagttggagt gacatgtgtg attggattcc atgaagggac gtattcaacc gaccagaaag tgagcgaggc gaaacgtgcc atgcagaatg gagccagcga attggatatg gtgatgaact acccttggct cagcgaaaaa cgctataccg acgtattcca ggatattcgc gcggtgagac ttgctgcaaa ggatgcgatt ttgaaggtca ttctcgagac atcccagttg accgcagatg agattatcgc cgggtgcgta ctctcgagtc tcgcaggtgc agactatgtg aagaccagta cgggttttaa cggtcctggt gcgagtattg aaaatgtttc gttgatgagt gctgtctgtg acagcttaca atcggagacc agggtgaaag ccagtggcgg cataaggaca atcgaagatt gcgtgaagat ggttagggca ggagcagaga gactaggcgc aagcgctggt gtgaagatcg tgaatgaaac tcggctggga aaccgtcaag tggatgaacc catggagcca acaaactaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for CoimA.00559.a.A1.PS00460
| PURIFICATION DATe: | 1/14/2010 |
| CONCENTRATION: | 14.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSLNNEEW DLLISGKKAT LQYPIPLLCY PAPEVVSIAQ IIDHTQLSLS ATGSQIDVLC AEAKEYGFAT VCVRPDYVSR AVQYLQGTQV GVTCVIGFHE GTYSTDQKVS EAKRAMQNGA SELDMVMNYP WLSEKRYTDV FQDIRAVRLA AKDAILKVIL ETSQLTADEI IAGCVLSSLA GADYVKTSTG FNGPGASIEN XSLMSAVCDS LQSETRVKAS GGIRTIEXCV KMVRAGAERL XASAGVKIVN ETRLGNRQVD EPM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgt ctctcaacaa cgaggaatgg gatttgctca tctcaggaaa gaaagctaca cttcaatatc ctattccact attatgctac cctgcccctg aggttgtttc aattgctcag ataattgatc acacccagct ctcgctctcc gccaccgggt ctcaaattga cgtcttgtgc gctgaagcga aagaatatgg ttttgcaacc gtatgtgtca ggccggacta tgtctctcgg gcagttcagt atcttcaagg aacccaagtt ggagtgacat gtgtgattgg attccatgaa gggacgtatt caaccgacca gaaagtgagc gaggcgaaac gtgccatgca gaatggagcc agcgaattgg atatggtgat gaactaccct tggctcagcg aaaaacgcta taccgacgta ttccaggata ttcgcgcggt gagacttgct gcaaaggatg cgattttgaa ggtcattctc gagacatccc agttgaccgc agatgagatt atcgccgggt gcgtactctc gagtctcgca ggtgcagact atgtgaagac cagtacgggt tttaacggtc ctggtgcgag tattgaaaat gnttcgttga tgagtgctgt ctgtgacagc ttacaatcgg agaccagggt gaaagccagt ggcggcataa ggacaatcga agnttgcgtg aagatggtta gggcaggagc agagagacta gncgcaagcg ctggtgtgaa gatcgtgaat gaaactcggc tgggaaaccg tcaagtggat gaacccatgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSLNNEEW DLLISGKKAT LQYPIPLLCY PAPEVVSIAQ IIDHTQLSLS ATGSQIDVLC AEAKEYGFAT VCVRPDYVSR AVQYLQGTQV GVTCVIGFHE GTYSTDQKVS EAKRAMQNGA SELDMVMNYP WLSEKRYTDV FQDIRAVRLA AKDAILKVIL ETSQLTADEI IAGCVLSSLA GADYVKTSTG FNGPGASIEN VSLMSAVCDS LQSETRVKAS GGIRTIEDCV KMVRAGAERL GASAGVKIVN ETRLGNRQVD EPMEPTNY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGTCTCTC AACAACGAGG AATGGGATTT GCTCATCTCA GGAAAGAAAG CTACACTTCA ATATCCTATT CCACTATTAT GCTACCCTGC CCCTGAGGTT GTTTCAATTG CTCAGATAAT TGATCACACC CAGCTCTCGC TCTCCGCCAC CGGGTCTCAA ATTGACGTCT TGTGCGCTGA AGCGAAAGAA TATGGTTTTG CAACCGTATG TGTCAGGCCG GACTATGTCT CTCGGGCAGT TCAGTATCTT CAAGGAACCC AAGTTGGAGT GACATGTGTG ATTGGATTCC ATGAAGGGAC GTATTCAACC GACCAGAAAG TGAGCGAGGC GAAACGTGCC ATGCAGAATG GAGCCAGCGA ATTGGATATG GTGATGAACT ACCCTTGGCT CAGCGAAAAA CGCTATACCG ACGTATTCCA GGATATTCGC GCGGTGAGAC TTGCTGCAAA GGATGCGATT TTGAAGGTCA TTCTCGAGAC ATCCCAGTTG ACCGCAGATG AGATTATCGC CGGGTGCGTA CTCTCGAGTC TCGCAGGTGC AGACTATGTG AAGACCAGTA CGGGTTTTAA CGGTCCTGGT GCGAGTATTG AAAATGTTTC GTTGATGAGT GCTGTCTGTG ACAGCTTACA ATCGGAGACC AGGGTGAAAG CCAGTGGCGG CATAAGGACA ATCGAAGATT GCGTGAAGAT GGTTAGGGCA GGAGCAGAGA GACTAGGCGC AAGCGCTGGT GTGAAGATCG TGAATGAAAC TCGGCTGGGA AACCGTCAAG TGGATGAACC CATGGAGCCA ACAAACTACA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A