CoimA.00479.a

Putative uncharacterized protein

CENTER ID:	CoimA.00479.a
ORGANISM:	Coccidioides immitis RS
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.00479.a.A1.GE30640	Full length( CoimA.00479.a )		1	433

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.00479.a.A1.PW30514	Full length( CoimA.00479.a )		1	433

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CIMG_01313
UniProt:	Q1E800

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASQSWLDIP KDSPFSLANI PFGIISTAVS ATPRPAIAIG DYALDLFTFA SAGGFSKLPA LQSHIDVFAQ PVLNDFAALG RPVHRQVREY LQEIFRDNTS HPEILKNNED LKKTALVPRK DATNHLPMRI GDYTDFYAGL NHAYNVGVLF RGPDNALQPN YKHLPVGYHG RASSVVVSGT SVRRPNGQIL ANPTADPKVP VFSPCKRLDI ELELAAFVAT PNKMGDPIPI SEAEDHLFGV VLMNDWSARD IQAWEYIPLG PFNAKNFATT ITPWIVLMDA LEPFRTQGLE PGNRDSLLPY LREKRADTAY DIDLEFELKN KGGQPTVVAK TNAKNLLYSF SQMLAHHSVT GCNMNTGDLL GSGTISGKEP GMYGSLLENT NGGKVAIKLA DGTERKFLED GDEVTLRGFA GQEGGYVGFG DCFSVIEPAH PIN

NT Sequence

ATGGCATCCC AATCCTGGCT GGACATCCCC AAGGATTCCC CTTTCTCTCT CGCAAACATC CCGTTCGGCA TCATTTCCAC CGCTGTCTCA GCGACACCAC GCCCCGCCAT TGCCATTGGC GACTATGCCC TGGACCTCTT CACTTTTGCC TCCGCTGGCG GTTTCTCCAA GCTGCCCGCT TTGCAGTCAC ACATTGATGT CTTTGCCCAG CCAGTGCTAA ACGACTTTGC CGCTCTGGGC AGACCTGTAC ACCGCCAGGT CAGAGAGTAC TTACAGGAGA TCTTCCGGGA TAACACCTCC CACCCCGAGA TCCTCAAGAA CAATGAAGAT CTCAAGAAAA CTGCCCTTGT TCCTCGGAAG GATGCCACCA ACCATCTTCC CATGAGGATC GGTGACTACA CCGATTTCTA TGCTGGTCTC AACCACGCCT ACAACGTCGG TGTGCTCTTC CGTGGCCCCG ATAATGCCTT GCAGCCAAAC TACAAGCATC TCCCCGTTGG TTATCACGGC CGCGCCTCGT CAGTGGTCGT GTCGGGCACT TCCGTCCGCC GACCCAACGG TCAGATCCTG GCCAACCCTA CGGCGGATCC CAAGGTGCCC GTTTTCTCTC CCTGCAAGCG ATTGGACATC GAACTCGAAC TGGCCGCCTT TGTGGCCACT CCCAACAAGA TGGGCGACCC GATCCCCATC TCTGAGGCCG AGGACCACCT GTTCGGTGTC GTCTTGATGA ACGACTGGTC TGCCCGCGAC ATCCAGGCCT GGGAGTACAT CCCATTGGGT CCGTTCAACG CTAAGAACTT CGCAACCACA ATCACACCTT GGATCGTCCT CATGGATGCC CTTGAGCCTT TCCGCACCCA GGGCCTTGAG CCAGGAAACC GTGATTCTCT CCTCCCGTAT CTCCGTGAAA AGAGAGCGGA CACCGCGTAC GATATCGATC TGGAATTCGA GCTGAAGAAC AAGGGCGGCC AGCCAACCGT CGTTGCCAAG ACCAACGCAA AGAACTTACT CTACTCCTTT TCGCAGATGT TGGCTCACCA CTCCGTCACC GGATGCAACA TGAACACTGG CGACTTGCTC GGTAGCGGTA CCATCTCCGG AAAGGAACCT GGCATGTACG GCAGCTTGTT GGAGAACACC AACGGTGGAA AGGTTGCCAT CAAGTTGGCA GACGGAACGG AGAGAAAGTT CTTGGAGGAT GGCGATGAGG TCACTCTGAG AGGATTCGCT GGCCAGGAGG GAGGTTACGT CGGATTCGGT GACTGCTTCT CAGTCATTGA ACCTGCTCAT CCAATTAAC

Details for CoimA.00479.a.A1.GE30640

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MASQSWLDIP KDSPFSLANI PFGIISTAVS ATPRPAIAIG DYALDLFTFA SAGGFSKLPA LQSHIDVFAQ PVLNDFAALG RPVHRQVREY LQEIFRDNTS HPEILKNNEX LKKTALVPRK DATNHLPMRI GDYTDFYAGL NHAYNVGVLF RGPDNALQPN YKHLPVGYHG RASSVVVSGT SVRRPNXQIL ANPXADPKVP VFSPCKRLDI ELELAAFVAT PNKMGDPIPI SEAEDHLFGV VLMNDWSARD IQAWEYIPLG PFNAKNFATT ITPWIVLMDA LEPFRTQGLE PGNRDSLLPY LREKRADTAY DIDLEFELKN KGGQPTVVAK TNAKNLLYSF SQMLAHHSVT GCNMNTGDLL GSGTISGKEP GMYGSLLENT NGGKVAIKLA DGTERKFLED GDEVTLRGFA GQEGGYVGFG DCFSVIEPAH PIN

Validated NT Sequence

tattagttaa ttggatgagc aggttcaatg actgagaagc agtcaccgaa tccgacgtaa cctccctcct ggccagcgaa tcctctcaga gtgacctcat cgccatcctc caagaacttt ctctccgttc cgtctgccaa cttgatggca acctttccac cgttggtgtt ctccaacaag ctgccgtaca tgccaggttc ctttccggag atggtaccgc taccgagcaa gtcgccagtg ttcatgttgc atccggtgac ggagtggtga gccaacatct gcgaaaagga gtagagtaag ttctttgcgt tggtcttggc aacgacggtt ggctggccgc ccttgttctt cagctcgaat tccagatcga tatcgtacgc ggtgtccgct ctcttttcac ggagatacgg gaggagagaa tcacggtttc ctggctcaag gccctgggtg cggaaaggct caagggcatc catgaggacg atccaaggtg tgattgtggt tgcgaagttc ttagcgttga acggacccaa tgggatgtac tcccaggcct ggatgtcgcg ggcagaccag tcgttcatca agacgacacc gaacaggtgg tcctcggcct cagagatggg gatcgggtcg cccatcttgt tgggagtggc cacaaaggcg gccagttcga gttcgatgtc caatcgcttg cagggagaga aaacgggcac cttgggatcc gccntagggt tggccaggat ctgacngttg ggtcggcgga cggaagtgcc cgacacgacc actgacgagg cgcggccgtg ataaccaacg gggagatgct tgtagtttgg ctgcaaggca ttatcggggc cacggaagag cacaccgacg ttgtaggcgt ggttgagacc agcatagaaa tcggtgtagt caccgatcct catgggaaga tggttggtgg catccttccg aggaacaagg gcagttttct tgagatnttc attgttcttg aggatctcgg ggtgggaggt gttatcccgg aagatctcct gtaagtactc tctgacctgg cggtgtacag gtctgcccag agcggcaaag tcgtttagca ctggctgggc aaagacatca atgtgtgact gcaaagcggg cagcttggag aaaccgccag cggaggcaaa agtgaagagg tccagggcat agtcgccaat ggcaatggcg gggcgtggtg tcgctgagac agcggtggaa atgatgccga acgggatgtt tgcgagagag aaaggggaat ccttggggat gtccagccag gattgggatg ccatcgaacc aggaccctgg gtctgagctt cca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASQSWLDI PKDSPFSLAN IPFGIISTAV SATPRPAIAI GDYALDLFTF ASAGGFSKLP ALQSHIDVFA QPVLNDFAAL GRPVHRQVRE YLQEIFRDNT SHPEILKNNE DLKKTALVPR KDATNHLPMR IGDYTDFYAG LNHAYNVGVL FRGPDNALQP NYKHLPVGYH GRASSVVVSG TSVRRPNGQI LANPTADPKV PVFSPCKRLD IELELAAFVA TPNKMGDPIP ISEAEDHLFG VVLMNDWSAR DIQAWEYIPL GPFNAKNFAT TITPWIVLMD ALEPFRTQGL EPGNRDSLLP YLREKRADTA YDIDLEFELK NKGGQPTVVA KTNAKNLLYS FSQMLAHHSV TGCNMNTGDL LGSGTISGKE PGMYGSLLEN TNGGKVAIKL ADGTERKFLE DGDEVTLRGF AGQEGGYVGF GDCFSVIEPA HPIN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatcccaa tcctggctgg acatccccaa ggattcccct ttctctctcg caaacatccc gttcggcatc atttccaccg ctgtctcagc gacaccacgc cccgccattg ccattggcga ctatgccctg gacctcttca cttttgcctc cgctggcggt ttctccaagc tgcccgcttt gcagtcacac attgatgtct ttgcccagcc agtgctaaac gactttgccg ctctgggcag acctgtacac cgccaggtca gagagtactt acaggagatc ttccgggata acacctccca ccccgagatc ctcaagaaca atgaagatct caagaaaact gcccttgttc ctcggaagga tgccaccaac catcttccca tgaggatcgg tgactacacc gatttctatg ctggtctcaa ccacgcctac aacgtcggtg tgctcttccg tggccccgat aatgccttgc agccaaacta caagcatctc cccgttggtt atcacggccg cgcctcgtca gtggtcgtgt cgggcacttc cgtccgccga cccaacggtc agatcctggc caaccctacg gcggatccca aggtgcccgt tttctctccc tgcaagcgat tggacatcga actcgaactg gccgcctttg tggccactcc caacaagatg ggcgacccga tccccatctc tgaggccgag gaccacctgt tcggtgtcgt cttgatgaac gactggtctg cccgcgacat ccaggcctgg gagtacatcc cattgggtcc gttcaacgct aagaacttcg caaccacaat cacaccttgg atcgtcctca tggatgccct tgagcctttc cgcacccagg gccttgagcc aggaaaccgt gattctctcc tcccgtatct ccgtgaaaag agagcggaca ccgcgtacga tatcgatctg gaattcgagc tgaagaacaa gggcggccag ccaaccgtcg ttgccaagac caacgcaaag aacttactct actccttttc gcagatgttg gctcaccact ccgtcaccgg atgcaacatg aacactggcg acttgctcgg tagcggtacc atctccggaa aggaacctgg catgtacggc agcttgttgg agaacaccaa cggtggaaag gttgccatca agttggcaga cggaacggag agaaagttct tggaggatgg cgatgaggtc actctgagag gattcgctgg ccaggaggga ggttacgtcg gattcggtga ctgcttctca gtcattgaac ctgctcatcc aattaacaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for CoimA.00479.a.A1.PW30514

PURIFICATION DATe:	12/20/2010
CONCENTRATION:	30.07mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATCCCAA TCCTGGCTGG ACATCCCCAA GGATTCCCCT TTCTCTCTCG CAAACATCCC GTTCGGCATC ATTTCCACCG CTGTCTCAGC GACACCACGC CCCGCCATTG CCATTGGCGA CTATGCCCTG GACCTCTTCA CTTTTGCCTC CGCTGGCGGT TTCTCCAAGC TGCCCGCTTT GCAGTCACAC ATTGATGTCT TTGCCCAGCC AGTGCTAAAC GACTTTGCCG CTCTGGGCAG ACCTGTACAC CGCCAGGTCA GAGAGTACTT ACAGGAGATC TTCCGGGATA ACACCTCCCA CCCCGAGATC CTCAAGAACA ATGAAGATCT CAAGAAAACT GCCCTTGTTC CTCGGAAGGA TGCCACCAAC CATCTTCCCA TGAGGATCGG TGACTACACC GATTTCTATG CTGGTCTCAA CCACGCCTAC AACGTCGGTG TGCTCTTCCG TGGCCCCGAT AATGCCTTGC AGCCAAACTA CAAGCATCTC CCCGTTGGTT ATCACGGCCG CGCCTCGTCA GTGGTCGTGT CGGGCACTTC CGTCCGCCGA CCCAACGGTC AGATCCTGGC CAACCCTACG GCGGATCCCA AGGTGCCCGT TTTCTCTCCC TGCAAGCGAT TGGACATCGA ACTCGAACTG GCCGCCTTTG TGGCCACTCC CAACAAGATG GGCGACCCGA TCCCCATCTC TGAGGCCGAG GACCACCTGT TCGGTGTCGT CTTGATGAAC GACTGGTCTG CCCGCGACAT CCAGGCCTGG GAGTACATCC CATTGGGTCC GTTCAACGCT AAGAACTTCG CAACCACAAT CACACCTTGG ATCGTCCTCA TGGATGCCCT TGAGCCTTTC CGCACCCAGG GCCTTGAGCC AGGAAACCGT GATTCTCTCC TCCCGTATCT CCGTGAAAAG AGAGCGGACA CCGCGTACGA TATCGATCTG GAATTCGAGC TGAAGAACAA GGGCGGCCAG CCAACCGTCG TTGCCAAGAC CAACGCAAAG AACTTACTCT ACTCCTTTTC GCAGATGTTG GCTCACCACT CCGTCACCGG ATGCAACATG AACACTGGCG ACTTGCTCGG TAGCGGTACC ATCTCCGGAA AGGAACCTGG CATGTACGGC AGCTTGTTGG AGAACACCAA CGGTGGAAAG GTTGCCATCA AGTTGGCAGA CGGAACGGAG AGAAAGTTCT TGGAGGATGG CGATGAGGTC ACTCTGAGAG GATTCGCTGG CCAGGAGGGA GGTTACGTCG GATTCGGTGA CTGCTTCTCA GTCATTGAAC CTGCTCATCC AATTAACAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA