CoimA.00478.a

Putative uncharacterized protein

CENTER ID: CoimA.00478.a
ORGANISM: Coccidioides immitis RS
ASSOCIATED DISEASE: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00478.a.A1.GE27317 Full length( CoimA.00478.a ) 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00478.a.A1.PW27308 Full length( CoimA.00478.a ) 1 491
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CIMG_01969
UniProt: Q1E644
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQMAPSQSL QAALSSVQEA FVKRNPQSNQ AHKEACRYFP GGNTRSVLYT TPFPLSFQSG HGSTLTSVDG DTYTDFLGEY SAGIFGHSNP QIAEALAGVL SRGWNFGGVN SHEAILARKV CERFNLEMVR FTNSGTEANM MALAAALAFS ARNHPGRPEP KSIVVFSNAY HGSTLSFPNT LVDEIRRGHD VSHLTPNLPH NFLIAPWNNP AETAELLATR PRGSIAAILV EPLQGAGGCR PASAHFLKTL RALADQKSAL LIVDEVMTSR LATRGLAQEW ALRPDLITLG KWIGGGMSFG AFGGRRDVLQ LFDPRTTKVP LGHAGTFNNN VLSMAAGCAG LDVYSGQRVA RLNELGSTLK ARLHALLADA SLCEPPANRT DNDNDDDDDD AQRYPLETDS NSDTNDVPPT PPPGRMYVSG YGSVLSIRFS GPDATTCHQL FFHHMLEEKI YLAARGYMAL SLELSRADIE HFIAAVQRFV TKYAALLLKD G
NT Sequence
ATGTCCCAAA TGGCACCATC CCAGTCCCTT CAAGCTGCCC TCTCCAGCGT CCAAGAGGCA TTCGTCAAAC GAAACCCCCA ATCCAACCAG GCCCACAAGG AAGCTTGTAG ATACTTCCCT GGCGGCAACA CACGCTCTGT GCTCTATACC ACCCCGTTCC CGCTGAGCTT CCAGTCGGGG CACGGCAGCA CCCTCACCTC TGTCGACGGG GACACATACA CCGATTTCCT GGGCGAGTAC AGCGCCGGTA TATTCGGCCA CTCGAACCCG CAGATCGCAG AGGCGCTCGC AGGCGTCCTG TCTCGCGGAT GGAACTTTGG CGGCGTGAAC TCACACGAGG CCATCCTCGC GCGCAAGGTC TGCGAGCGTT TCAATCTTGA AATGGTCCGT TTCACGAACT CGGGCACGGA GGCAAACATG ATGGCCCTGG CCGCCGCACT GGCCTTCTCC GCCAGGAACC ATCCCGGCCG CCCGGAGCCC AAATCCATCG TGGTCTTCAG CAACGCCTAT CACGGCAGCA CCCTCTCCTT TCCAAACACC CTCGTGGACG AGATCCGTCG CGGCCACGAT GTATCTCACC TCACGCCCAA CCTACCCCAC AACTTCCTCA TCGCGCCCTG GAACAACCCT GCCGAGACCG CCGAGCTCCT AGCCACCCGG CCCCGGGGCT CCATCGCCGC GATCCTCGTC GAACCCCTCC AGGGCGCCGG CGGCTGCCGT CCGGCATCAG CGCACTTCCT CAAGACCCTC CGAGCGCTAG CAGACCAGAA ATCCGCCCTG CTCATCGTCG ACGAGGTGAT GACCTCCCGC CTCGCCACCC GTGGGCTCGC CCAAGAATGG GCCCTCAGGC CCGACCTGAT CACCCTGGGC AAATGGATCG GCGGGGGTAT GAGCTTCGGC GCGTTCGGCG GACGCAGAGA CGTACTGCAG CTCTTCGACC CGCGGACGAC GAAGGTTCCG CTCGGCCACG CGGGTACGTT CAACAATAAC GTGCTCAGCA TGGCGGCGGG CTGCGCGGGG CTGGACGTCT ACTCCGGCCA ACGCGTCGCG CGGCTGAACG AGCTCGGATC GACGCTGAAA GCGAGACTGC ACGCCCTCCT GGCTGATGCA TCGCTGTGCG AGCCTCCTGC CAACCGCACC GACAACGACA ACGATGATGA CGATGATGAT GCGCAAAGAT ATCCCCTAGA AACAGATTCC AACAGCGACA CCAACGACGT ACCTCCCACA CCACCCCCAG GACGGATGTA CGTCTCCGGC TACGGCAGCG TCCTCAGCAT CCGTTTCTCG GGCCCCGACG CCACGACCTG CCACCAGCTG TTCTTCCACC ACATGCTCGA AGAGAAGATC TACCTCGCTG CGCGCGGCTA CATGGCGCTA TCGCTCGAAT TAAGTCGCGC AGACATCGAG CATTTCATCG CCGCCGTGCA AAGATTCGTG ACCAAGTACG CTGCTCTGTT ATTAAAGGAT GGA
Details for CoimA.00478.a.A1.GE27317
HARVESTED ON: 10/23/2009
SEQUENCED ON: 10/29/2009
EXPECTED MW: 55kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQMAPSQS LQAALSSVQE AFVKRNPQSN QAHKEACRYF PGGNTRSVLY TTPFPLSFQS GHGSTLTSVD GDTYTDFLGE YSAGIFGHSN PQIAEALAGV LSRGWNFGGV NSHEAILARK VCERFNLEMV RFTNSGTEAN MMALAAALAF SARNHPGRPE PKSIVVFSNA YHGSTLSFPN TLVDEIRRGH DVSHLTPNLP HNFLIAPWNN PAETAELLAT RPRGSIAAIL VEPLQGAGGC RPASAHFLKT LRALADQKSA LLIVDEVMTS RLATRGLAQE WALRPDLITL GKWIGGGMSF GAFGGRRDVL QLFDPRTTKV PLGHAGTFNN NVLSMAAGCA GLDVYSGQRV ARLNELGSTL KARLHALLAD ASLCEPPANR TDNDNDDDDD DAQRYPLETD SNSDTNDVPP TPPPGRMYVS GYGSVLSIRF SGPDATTCHQ LFFHHMLEEK IYLAARGYMA LSLELSRADI EHFIAAVQRF VTKYAALLLK
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccca aatggcacca tcccagtccc ttcaagctgc cctctccagc gtccaagagg cattcgtcaa acgaaacccc caatccaacc aggcccacaa ggaagcttgt agatacttcc ctggcggcaa cacacgctct gtgctctata ccaccccgtt cccgctgagc ttccagtcgg ggcacggcag caccctcacc tctgtcgacg gggacacata caccgatttc ctgggcgagt acagcgccgg tatattcggc cactcgaacc cgcagatcgc agaggcgctc gcaggcgtcc tgtctcgcgg atggaacttt ggcggcgtga actcacacga ggccatcctc gcgcgcaagg tctgcgagcg tttcaatctt gaaatggtcc gtttcacgaa ctcgggcacg gaggcaaaca tgatggccct ggccgccgca ctggccttct ccgccaggaa ccatcccggc cgcccggagc ccaaatccat cgtggtcttc agcaacgcct atcacggcag caccctctcc tttccaaaca ccctcgtgga cgagatccgt cgcggccacg atgtatctca cctcacgccc aacctacccc acaacttcct catcgcgccc tggaacaacc ctgccgagac cgccgagctc ctagccaccc ggccccgggg ctccatcgcc gcgatcctcg tcgaacccct ccagggcgcc ggcggctgcc gtccggcatc agcgcacttc ctcaagaccc tccgagcgct agcagaccag aaatccgccc tgctcatcgt cgacgaggtg atgacctccc gcctcgccac ccgtgggctc gcccaagaat gggccctcag gcccgacctg atcaccctgg gcaaatggat cggcgggggt atgagcttcg gcgcgttcgg cggacgcaga gacgtactgc agctcttcga cccgcggacg acgaaggttc cgctcggcca cgcgggtacg ttcaacaata acgtgctcag catggcggcg ggctgcgcgg ggctggacgt ctactccggc caacgcgtcg cgcggctgaa cgagctcgga tcgacgctga aagcgagact gcacgccctc ctggctgatg catcgctgtg cgagcctcct gccaaccgca ccgacaacga caacgatgat gacgatgatg atgcgcaaag atatccccta gaaacagatt ccaacagcga caccaacgac gtacctccca caccaccccc aggacggatg tacgtctccg gctacggcag cgtcctcagc atccgtttct cgggccccga cgccacgacc tgccaccagc tgttcttcca ccacatgctc gaagagaaga tctacctcgc tgcgcgcggc tacatggcgc tatcgctcga attaagtcgc gcagacatcg agcatttcat cgccgccgtg caaagattcg tgaccaagta cgctgctctg ttattaaagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQMAPSQS LQAALSSVQE AFVKRNPQSN QAHKEACRYF PGGNTRSVLY TTPFPLSFQS GHGSTLTSVD GDTYTDFLGE YSAGIFGHSN PQIAEALAGV LSRGWNFGGV NSHEAILARK VCERFNLEMV RFTNSGTEAN MMALAAALAF SARNHPGRPE PKSIVVFSNA YHGSTLSFPN TLVDEIRRGH DVSHLTPNLP HNFLIAPWNN PAETAELLAT RPRGSIAAIL VEPLQGAGGC RPASAHFLKT LRALADQKSA LLIVDEVMTS RLATRGLAQE WALRPDLITL GKWIGGGMSF GAFGGRRDVL QLFDPRTTKV PLGHAGTFNN NVLSMAAGCA GLDVYSGQRV ARLNELGSTL KARLHALLAD ASLCEPPANR TDNDNDDDDD DAQRYPLETD SNSDTNDVPP TPPPGRMYVS GYGSVLSIRF SGPDATTCHQ LFFHHMLEEK IYLAARGYMA LSLELSRADI EHFIAAVQRF VTKYAALLLK DG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcccaaatg gcaccatccc agtcccttca agctgccctc tccagcgtcc aagaggcatt cgtcaaacga aacccccaat ccaaccaggc ccacaaggaa gcttgtagat acttccctgg cggcaacaca cgctctgtgc tctataccac cccgttcccg ctgagcttcc agtcggggca cggcagcacc ctcacctctg tcgacgggga cacatacacc gatttcctgg gcgagtacag cgccggtata ttcggccact cgaacccgca gatcgcagag gcgctcgcag gcgtcctgtc tcgcggatgg aactttggcg gcgtgaactc acacgaggcc atcctcgcgc gcaaggtctg cgagcgtttc aatcttgaaa tggtccgttt cacgaactcg ggcacggagg caaacatgat ggccctggcc gccgcactgg ccttctccgc caggaaccat cccggccgcc cggagcccaa atccatcgtg gtcttcagca acgcctatca cggcagcacc ctctcctttc caaacaccct cgtggacgag atccgtcgcg gccacgatgt atctcacctc acgcccaacc taccccacaa cttcctcatc gcgccctgga acaaccctgc cgagaccgcc gagctcctag ccacccggcc ccggggctcc atcgccgcga tcctcgtcga acccctccag ggcgccggcg gctgccgtcc ggcatcagcg cacttcctca agaccctccg agcgctagca gaccagaaat ccgccctgct catcgtcgac gaggtgatga cctcccgcct cgccacccgt gggctcgccc aagaatgggc cctcaggccc gacctgatca ccctgggcaa atggatcggc gggggtatga gcttcggcgc gttcggcgga cgcagagacg tactgcagct cttcgacccg cggacgacga aggttccgct cggccacgcg ggtacgttca acaataacgt gctcagcatg gcggcgggct gcgcggggct ggacgtctac tccggccaac gcgtcgcgcg gctgaacgag ctcggatcga cgctgaaagc gagactgcac gccctcctgg ctgatgcatc gctgtgcgag cctcctgcca accgcaccga caacgacaac gatgatgacg atgatgatgc gcaaagatat cccctagaaa cagattccaa cagcgacacc aacgacgtac ctcccacacc acccccagga cggatgtacg tctccggcta cggcagcgtc ctcagcatcc gtttctcggg ccccgacgcc acgacctgcc accagctgtt cttccaccac atgctcgaag agaagatcta cctcgctgcg cgcggctaca tggcgctatc gctcgaatta agtcgcgcag acatcgagca tttcatcgcc gccgtgcaaa gattcgtgac caagtacgct gctctgttat taaaggatgg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for CoimA.00478.a.A1.PW27308
PURIFICATION DATe: 12/14/2009
CONCENTRATION: 25.74mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 19
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQMAPSQS LQAALSSVQE AFVKRNPQSN QAHKEACRYF PGGNTRSVLY TTPFPLSFQS GHGSTLTSVD GDTYTDFLGE YSAGIFGHSN PQIAEALAGV LSRGWNFGGV NSHEAILARK VCERFNLEMV RFTNSGTEAN MMALAAALAF SARNHPGRPE PKSIVVFSNA YHGSTLSFPN TLVDEIRRGH DVSHLTPNLP HNFLIAPWNN PAETAELLAT RPRGSIAAIL VEPLQGAGGC RPASAHFLKT LRALADQKSA LLIVDEVMTS RLATRGLAQE WALRPDLITL GKWIGGGMSF GAFGGRRDVL QLFDPRTTKV PLGHAGTFNN NVLSMAAGCA GLDVYSGQRV ARLNELGSTL KARLHALLAD ASLCEPPANR TDNDNDDDDD DAQRYPLETD SNSDTNDVPP TPPPGRMYVS GYGSVLSIRF SGPDATTCHQ LFFHHMLEEK IYLAARGYMA LSLELSRADI EHFIAAVQRF VTKYAALLLK
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccca aatggcacca tcccagtccc ttcaagctgc cctctccagc gtccaagagg cattcgtcaa acgaaacccc caatccaacc aggcccacaa ggaagcttgt agatacttcc ctggcggcaa cacacgctct gtgctctata ccaccccgtt cccgctgagc ttccagtcgg ggcacggcag caccctcacc tctgtcgacg gggacacata caccgatttc ctgggcgagt acagcgccgg tatattcggc cactcgaacc cgcagatcgc agaggcgctc gcaggcgtcc tgtctcgcgg atggaacttt ggcggcgtga actcacacga ggccatcctc gcgcgcaagg tctgcgagcg tttcaatctt gaaatggtcc gtttcacgaa ctcgggcacg gaggcaaaca tgatggccct ggccgccgca ctggccttct ccgccaggaa ccatcccggc cgcccggagc ccaaatccat cgtggtcttc agcaacgcct atcacggcag caccctctcc tttccaaaca ccctcgtgga cgagatccgt cgcggccacg atgtatctca cctcacgccc aacctacccc acaacttcct catcgcgccc tggaacaacc ctgccgagac cgccgagctc ctagccaccc ggccccgggg ctccatcgcc gcgatcctcg tcgaacccct ccagggcgcc ggcggctgcc gtccggcatc agcgcacttc ctcaagaccc tccgagcgct agcagaccag aaatccgccc tgctcatcgt cgacgaggtg atgacctccc gcctcgccac ccgtgggctc gcccaagaat gggccctcag gcccgacctg atcaccctgg gcaaatggat cggcgggggt atgagcttcg gcgcgttcgg cggacgcaga gacgtactgc agctcttcga cccgcggacg acgaaggttc cgctcggcca cgcgggtacg ttcaacaata acgtgctcag catggcggcg ggctgcgcgg ggctggacgt ctactccggc caacgcgtcg cgcggctgaa cgagctcgga tcgacgctga aagcgagact gcacgccctc ctggctgatg catcgctgtg cgagcctcct gccaaccgca ccgacaacga caacgatgat gacgatgatg atgcgcaaag atatccccta gaaacagatt ccaacagcga caccaacgac gtacctccca caccaccccc aggacggatg tacgtctccg gctacggcag cgtcctcagc atccgtttct cgggccccga cgccacgacc tgccaccagc tgttcttcca ccacatgctc gaagagaaga tctacctcgc tgcgcgcggc tacatggcgc tatcgctcga attaagtcgc gcagacatcg agcatttcat cgccgccgtg caaagattcg tgaccaagta cgctgctctg ttattaaagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQMAPSQS LQAALSSVQE AFVKRNPQSN QAHKEACRYF PGGNTRSVLY TTPFPLSFQS GHGSTLTSVD GDTYTDFLGE YSAGIFGHSN PQIAEALAGV LSRGWNFGGV NSHEAILARK VCERFNLEMV RFTNSGTEAN MMALAAALAF SARNHPGRPE PKSIVVFSNA YHGSTLSFPN TLVDEIRRGH DVSHLTPNLP HNFLIAPWNN PAETAELLAT RPRGSIAAIL VEPLQGAGGC RPASAHFLKT LRALADQKSA LLIVDEVMTS RLATRGLAQE WALRPDLITL GKWIGGGMSF GAFGGRRDVL QLFDPRTTKV PLGHAGTFNN NVLSMAAGCA GLDVYSGQRV ARLNELGSTL KARLHALLAD ASLCEPPANR TDNDNDDDDD DAQRYPLETD SNSDTNDVPP TPPPGRMYVS GYGSVLSIRF SGPDATTCHQ LFFHHMLEEK IYLAARGYMA LSLELSRADI EHFIAAVQRF VTKYAALLLK DG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCCAAATG GCACCATCCC AGTCCCTTCA AGCTGCCCTC TCCAGCGTCC AAGAGGCATT CGTCAAACGA AACCCCCAAT CCAACCAGGC CCACAAGGAA GCTTGTAGAT ACTTCCCTGG CGGCAACACA CGCTCTGTGC TCTATACCAC CCCGTTCCCG CTGAGCTTCC AGTCGGGGCA CGGCAGCACC CTCACCTCTG TCGACGGGGA CACATACACC GATTTCCTGG GCGAGTACAG CGCCGGTATA TTCGGCCACT CGAACCCGCA GATCGCAGAG GCGCTCGCAG GCGTCCTGTC TCGCGGATGG AACTTTGGCG GCGTGAACTC ACACGAGGCC ATCCTCGCGC GCAAGGTCTG CGAGCGTTTC AATCTTGAAA TGGTCCGTTT CACGAACTCG GGCACGGAGG CAAACATGAT GGCCCTGGCC GCCGCACTGG CCTTCTCCGC CAGGAACCAT CCCGGCCGCC CGGAGCCCAA ATCCATCGTG GTCTTCAGCA ACGCCTATCA CGGCAGCACC CTCTCCTTTC CAAACACCCT CGTGGACGAG ATCCGTCGCG GCCACGATGT ATCTCACCTC ACGCCCAACC TACCCCACAA CTTCCTCATC GCGCCCTGGA ACAACCCTGC CGAGACCGCC GAGCTCCTAG CCACCCGGCC CCGGGGCTCC ATCGCCGCGA TCCTCGTCGA ACCCCTCCAG GGCGCCGGCG GCTGCCGTCC GGCATCAGCG CACTTCCTCA AGACCCTCCG AGCGCTAGCA GACCAGAAAT CCGCCCTGCT CATCGTCGAC GAGGTGATGA CCTCCCGCCT CGCCACCCGT GGGCTCGCCC AAGAATGGGC CCTCAGGCCC GACCTGATCA CCCTGGGCAA ATGGATCGGC GGGGGTATGA GCTTCGGCGC GTTCGGCGGA CGCAGAGACG TACTGCAGCT CTTCGACCCG CGGACGACGA AGGTTCCGCT CGGCCACGCG GGTACGTTCA ACAATAACGT GCTCAGCATG GCGGCGGGCT GCGCGGGGCT GGACGTCTAC TCCGGCCAAC GCGTCGCGCG GCTGAACGAG CTCGGATCGA CGCTGAAAGC GAGACTGCAC GCCCTCCTGG CTGATGCATC GCTGTGCGAG CCTCCTGCCA ACCGCACCGA CAACGACAAC GATGATGACG ATGATGATGC GCAAAGATAT CCCCTAGAAA CAGATTCCAA CAGCGACACC AACGACGTAC CTCCCACACC ACCCCCAGGA CGGATGTACG TCTCCGGCTA CGGCAGCGTC CTCAGCATCC GTTTCTCGGG CCCCGACGCC ACGACCTGCC ACCAGCTGTT CTTCCACCAC ATGCTCGAAG AGAAGATCTA CCTCGCTGCG CGCGGCTACA TGGCGCTATC GCTCGAATTA AGTCGCGCAG ACATCGAGCA TTTCATCGCC GCCGTGCAAA GATTCGTGAC CAAGTACGCT GCTCTGTTAT TAAAGGATGG AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA