CoimA.00464.a

Calmodulin

CENTER ID:	CoimA.00464.a
ORGANISM:	Coccidioides immitis RS
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CoimA.00464.a.FX11.GE00470	full length		18	166

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CIMG_02413
UniProt:	J3KLB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADSLTEEQV SEYKEAFSLF DKDGDGQITT KELGTVMRSL GQNPSESELQ DMINEVDADN NGTIDFPEFL TMMARKMKDT DSEEEIREAF KVFDRDNNGF ISAAELRHVM TSIGEKLTDD EVDEMIREAD QDGDGRIDYN EFVQLMMQK

NT Sequence

atggctgatt cattgaccga agaacaagtt tctgagtaca aggaggcgtt ctctctgttt gacaaagatg gcgatggtca gatcaccacc aaggaactcg gaaccgtaat gcgttccctc ggccaaaacc cttccgaatc tgaattacag gatatgatca acgaggttga cgccgataac aatggaacca tcgatttccc ggagttcctt accatgatgg cacggaaaat gaaggatacc gattccgaag aagagattcg ggaggcgttc aaggtctttg accgggataa caacggcttc atctccgccg cagagttgcg ccacgtaatg acctccatcg gtgagaagtt gaccgatgac gaagtggatg agatgatccg tgaggccgac caggatggtg atggaagaat cgactataac gaattcgtcc aacttatgat gcagaaataa

Details for CoimA.00464.a.FX11.GE00470

HARVESTED ON:	8/10/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	17kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Data Unavailable
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MADSLTEEQV SEYKEAFSLF DKDGDGQITT KELGTVMRSL GQNPSESELQ DMINEVDADN NGTIDFPEFL TMMARKMKDT DSEEEIREAF KVFDRDNNGF ISAAELRHVM TSIGEKLTDD EVDEMIREAD QDGDGRIDYN EFVQLMMQK

Full NT Sequence (Expression Vector + Insert)

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatccatg gcggactcat tgaccgaaga acaagtgtct gagtataagg aagccttctc tcttttcgac aaggacggcg atggacagat cacgacaaag gagctgggca ctgtgatgcg tagtcttggt cagaatccta gcgagagcga gctgcaggat atgattaatg aggtcgatgc agataacaac ggaactatag atttccccga gttcttgaca atgatggccc ggaagatgaa ggacaccgat tctgaggagg agattaggga ggcttttaag gttttcgatc gggataacaa tggcttcatc tccgctgccg aactccgcca cgtgatgaca tcaattggag aaaagctgac tgatgatgaa gtggacgaga tgatcagaga ggccgatcag gatggggatg gtcgcattga ctacaatgag tttgtacagc tgatgatgca gaaatgataa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg