CoimA.00447.a
Putative 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (EC=4.1.3.16) (Dihydrodipicolinate synthase-like) (DHDPS-like protein)
| CENTER ID: | CoimA.00447.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00447.a.A1.GE27316 | Full length( CoimA.00447.a ) | 1 | 314 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00447.a.A1.PW28367 | Full length( CoimA.00447.a ) | 1 | 314 |
Structures
3QFE
DEPOSITED: 1/21/2011
DETERMINATION: XRay
CLONE: CoimA.00447.a.A1.GE27316
PROTEIN: CoimA.00447.a.A1.PW28367
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_00151 |
| OrthoMCL: | OG5_144400 |
| RefSeq: | XP_001246380.1 |
| UniProt: | P0CL20 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVPRVPQPGI WCPAVTFFDS KTDTLDLASQ ERYYAYLARS GLTGLVILGT NAEAFLLTRE ERAQLIATAR KAVGPDFPIM AGVGAHSTRQ VLEHINDASV AGANYVLVLP PAYFGKATTP PVIKSFFDDV SCQSPLPVVI YNFPGVCNGI DLDSDMITTI ARKNPNVVGV KLTCASVGKI TRLAATLPPA AFSVFGGQSD FLIGGLSVGS AGCIAAFANV FPKTVSKIYE LYKAGKVDQA MELHRKAALA ESPCKSGIAT TKYAAAIFSA KAAGIEDAEE KLRPRKPYDP PSEAAKQEVR KVMAEVAAIE AGLS
NT Sequence
ATGGTCCCTC GCGTCCCGCA ACCGGGCATC TGGTGCCCCG CAGTGACCTT CTTCGACTCC AAGACAGACA CCCTCGACTT GGCCTCGCAG GAGAGGTACT ACGCATACCT GGCTCGCTCT GGGCTGACCG GTCTTGTCAT TCTCGGAACA AATGCAGAGG CATTCCTGCT CACGCGTGAA GAGCGTGCCC AGCTCATCGC CACAGCCCGT AAAGCCGTGG GGCCAGATTT CCCCATCATG GCCGGCGTAG GCGCCCATTC AACGCGGCAG GTCCTCGAAC ACATCAACGA CGCATCCGTG GCGGGAGCAA ACTATGTGCT TGTCCTGCCG CCTGCATACT TTGGAAAGGC CACCACTCCT CCGGTGATAA AATCATTCTT TGACGACGTT TCCTGCCAAT CGCCGCTTCC GGTGGTGATC TATAACTTCC CAGGCGTGTG CAACGGAATC GATCTGGATT CGGATATGAT CACGACCATC GCGAGGAAGA ACCCCAACGT CGTCGGCGTG AAACTCACCT GTGCCAGCGT GGGCAAGATC ACGCGGCTCG CAGCGACACT GCCACCAGCA GCATTCTCTG TCTTTGGTGG GCAGTCCGAT TTCTTGATCG GTGGGTTGAG CGTGGGTTCG GCGGGATGCA TTGCCGCGTT TGCGAATGTA TTCCCAAAGA CTGTTTCCAA GATATATGAG CTGTATAAAG CCGGAAAGGT GGACCAGGCG ATGGAATTGC ATCGCAAAGC TGCGTTAGCG GAGTCGCCGT GTAAATCTGG GATTGCCACA ACGAAGTACG CAGCTGCGAT CTTTTCAGCA AAAGCGGCTG GGATTGAAGA TGCGGAGGAA AAGTTGCGAC CACGCAAGCC CTATGATCCA CCGAGTGAAG CGGCAAAGCA GGAAGTGAGA AAGGTGATGG CCGAAGTTGC GGCGATAGAG GCGGGCTTAT CC
Details for CoimA.00447.a.A1.GE27316
| HARVESTED ON: | 10/23/2009 |
| SEQUENCED ON: | 10/29/2009 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMVPRVPQ PGIWCPAVTF FDSKTDTLDL ASQERYYAYL ARSGLTGLVI LGTNAEAFLL TREERAQLIA TARKAVGPDF PIMAGVGAHS TRQVLEHIND ASVAGANYVL VLPPAYFGKA TTPPVIKSFF DDVSCQSPLP VVIYNFPGVC NGIDLDSDMI TTIARKNPNV VGVKLTCASV GKITRLAATL PPAAFSVFGG QSDFLIGGLS VGSAGCIAAF ANVFPKTVSK IYELYKAGKV DQAMELHRKA ALAESPCKSG IATTKYAAAI FSAKAAGIED AEEKLRPRKP YDPPSEAAKQ EVRKVMAEVA AIEAGLS
Validated NT Sequence
atatgggtac cctggaagct cagacccagg gtcctggttc gatggtccct cgcgtcccgc aaccgggcat ctggtgcccc gcagtgacct tcttcgactc caagacagac accctcgact tggcctcgca ggagaggtac tacgcatacc tggctcgctc tgggctgacc ggtcttgtca ttctcggaac aaatgcagag gcattcctgc tcacgcgtga agagcgtgcc cagctcatcg ccacagcccg taaagccgtg gggccagatt tccccatcat ggccggcgta ggcgcccatt caacgcggca ggtcctcgaa cacatcaacg acgcatccgt ggcgggagca aactatgtgc ttgtcctgcc gcctgcatac tttggaaagg ccaccactcc tccggtgata aaatcattct ttgacgacgt ttcctgccaa tcgccgcttc cggtggtgat ctataacttc ccaggcgtgt gcaacggaat cgatctggat tcggatatga tcacgaccat cgcgaggaag aaccccaacg tcgtcggcgt gaaactcacc tgtgccagcg tgggcaagat cacgcggctc gcagcgacac tgccaccagc agcattctct gtctttggtg ggcagtccga tttcttgatc ggtgggttga gcgtgggttc ggcgggatgc attgccgcgt ttgcgaatgt attcccaaag actgtttcca agatatatga gctgtataaa gccggaaagg tggaccaggc gatggaattg catcgcaaag ctgcgttagc ggagtcgccg tgtaaatctg ggattgccac aacgaagtac gcagctgcga tcttttcagc aaaagcggct gggattgaag atgcggagga aaagttgcga ccacgcaagc cctatgatcc accgagtgaa gcggcaaagc aggaagtgag aaaggtgatg gccgaagttg cggcgataga ggcgggctta tcctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPRVPQPG IWCPAVTFFD SKTDTLDLAS QERYYAYLAR SGLTGLVILG TNAEAFLLTR EERAQLIATA RKAVGPDFPI MAGVGAHSTR QVLEHINDAS VAGANYVLVL PPAYFGKATT PPVIKSFFDD VSCQSPLPVV IYNFPGVCNG IDLDSDMITT IARKNPNVVG VKLTCASVGK ITRLAATLPP AAFSVFGGQS DFLIGGLSVG SAGCIAAFAN VFPKTVSKIY ELYKAGKVDQ AMELHRKAAL AESPCKSGIA TTKYAAAIFS AKAAGIEDAE EKLRPRKPYD PPSEAAKQEV RKVMAEVAAI EAGLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtccctcgc gtcccgcaac cgggcatctg gtgccccgca gtgaccttct tcgactccaa gacagacacc ctcgacttgg cctcgcagga gaggtactac gcatacctgg ctcgctctgg gctgaccggt cttgtcattc tcggaacaaa tgcagaggca ttcctgctca cgcgtgaaga gcgtgcccag ctcatcgcca cagcccgtaa agccgtgggg ccagatttcc ccatcatggc cggcgtaggc gcccattcaa cgcggcaggt cctcgaacac atcaacgacg catccgtggc gggagcaaac tatgtgcttg tcctgccgcc tgcatacttt ggaaaggcca ccactcctcc ggtgataaaa tcattctttg acgacgtttc ctgccaatcg ccgcttccgg tggtgatcta taacttccca ggcgtgtgca acggaatcga tctggattcg gatatgatca cgaccatcgc gaggaagaac cccaacgtcg tcggcgtgaa actcacctgt gccagcgtgg gcaagatcac gcggctcgca gcgacactgc caccagcagc attctctgtc tttggtgggc agtccgattt cttgatcggt gggttgagcg tgggttcggc gggatgcatt gccgcgtttg cgaatgtatt cccaaagact gtttccaaga tatatgagct gtataaagcc ggaaaggtgg accaggcgat ggaattgcat cgcaaagctg cgttagcgga gtcgccgtgt aaatctggga ttgccacaac gaagtacgca gctgcgatct tttcagcaaa agcggctggg attgaagatg cggaggaaaa gttgcgacca cgcaagccct atgatccacc gagtgaagcg gcaaagcagg aagtgagaaa ggtgatggcc gaagttgcgg cgatagaggc gggcttatcc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for CoimA.00447.a.A1.PW28367
| PURIFICATION DATe: | 12/7/2009 |
| CONCENTRATION: | 24.64mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVPRVPQ PGIWCPAVTF FDSKTDTLDL ASQERYYAYL ARSGLTGLVI LGTNAEAFLL TREERAQLIA TARKAVGPDF PIMAGVGAHS TRQVLEHIND ASVAGANYVL VLPPAYFGKA TTPPVIKSFF DDVSCQSPLP VVIYNFPGVC NGIDLDSDMI TTIARKNPNV VGVKLTCASV GKITRLAATL PPAAFSVFGG QSDFLIGGLS VGSAGCIAAF ANVFPKTVSK IYELYKAGKV DQAMELHRKA ALAESPCKSG IATTKYAAAI FSAKAAGIED AEEKLRPRKP YDPPSEAAKQ EVRKVMAEVA AIEAGLS
Validated NT Sequence
atatgggtac cctggaagct cagacccagg gtcctggttc gatggtccct cgcgtcccgc aaccgggcat ctggtgcccc gcagtgacct tcttcgactc caagacagac accctcgact tggcctcgca ggagaggtac tacgcatacc tggctcgctc tgggctgacc ggtcttgtca ttctcggaac aaatgcagag gcattcctgc tcacgcgtga agagcgtgcc cagctcatcg ccacagcccg taaagccgtg gggccagatt tccccatcat ggccggcgta ggcgcccatt caacgcggca ggtcctcgaa cacatcaacg acgcatccgt ggcgggagca aactatgtgc ttgtcctgcc gcctgcatac tttggaaagg ccaccactcc tccggtgata aaatcattct ttgacgacgt ttcctgccaa tcgccgcttc cggtggtgat ctataacttc ccaggcgtgt gcaacggaat cgatctggat tcggatatga tcacgaccat cgcgaggaag aaccccaacg tcgtcggcgt gaaactcacc tgtgccagcg tgggcaagat cacgcggctc gcagcgacac tgccaccagc agcattctct gtctttggtg ggcagtccga tttcttgatc ggtgggttga gcgtgggttc ggcgggatgc attgccgcgt ttgcgaatgt attcccaaag actgtttcca agatatatga gctgtataaa gccggaaagg tggaccaggc gatggaattg catcgcaaag ctgcgttagc ggagtcgccg tgtaaatctg ggattgccac aacgaagtac gcagctgcga tcttttcagc aaaagcggct gggattgaag atgcggagga aaagttgcga ccacgcaagc cctatgatcc accgagtgaa gcggcaaagc aggaagtgag aaaggtgatg gccgaagttg cggcgataga ggcgggctta tcctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPRVPQPG IWCPAVTFFD SKTDTLDLAS QERYYAYLAR SGLTGLVILG TNAEAFLLTR EERAQLIATA RKAVGPDFPI MAGVGAHSTR QVLEHINDAS VAGANYVLVL PPAYFGKATT PPVIKSFFDD VSCQSPLPVV IYNFPGVCNG IDLDSDMITT IARKNPNVVG VKLTCASVGK ITRLAATLPP AAFSVFGGQS DFLIGGLSVG SAGCIAAFAN VFPKTVSKIY ELYKAGKVDQ AMELHRKAAL AESPCKSGIA TTKYAAAIFS AKAAGIEDAE EKLRPRKPYD PPSEAAKQEV RKVMAEVAAI EAGLS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTCCCTCGC GTCCCGCAAC CGGGCATCTG GTGCCCCGCA GTGACCTTCT TCGACTCCAA GACAGACACC CTCGACTTGG CCTCGCAGGA GAGGTACTAC GCATACCTGG CTCGCTCTGG GCTGACCGGT CTTGTCATTC TCGGAACAAA TGCAGAGGCA TTCCTGCTCA CGCGTGAAGA GCGTGCCCAG CTCATCGCCA CAGCCCGTAA AGCCGTGGGG CCAGATTTCC CCATCATGGC CGGCGTAGGC GCCCATTCAA CGCGGCAGGT CCTCGAACAC ATCAACGACG CATCCGTGGC GGGAGCAAAC TATGTGCTTG TCCTGCCGCC TGCATACTTT GGAAAGGCCA CCACTCCTCC GGTGATAAAA TCATTCTTTG ACGACGTTTC CTGCCAATCG CCGCTTCCGG TGGTGATCTA TAACTTCCCA GGCGTGTGCA ACGGAATCGA TCTGGATTCG GATATGATCA CGACCATCGC GAGGAAGAAC CCCAACGTCG TCGGCGTGAA ACTCACCTGT GCCAGCGTGG GCAAGATCAC GCGGCTCGCA GCGACACTGC CACCAGCAGC ATTCTCTGTC TTTGGTGGGC AGTCCGATTT CTTGATCGGT GGGTTGAGCG TGGGTTCGGC GGGATGCATT GCCGCGTTTG CGAATGTATT CCCAAAGACT GTTTCCAAGA TATATGAGCT GTATAAAGCC GGAAAGGTGG ACCAGGCGAT GGAATTGCAT CGCAAAGCTG CGTTAGCGGA GTCGCCGTGT AAATCTGGGA TTGCCACAAC GAAGTACGCA GCTGCGATCT TTTCAGCAAA AGCGGCTGGG ATTGAAGATG CGGAGGAAAA GTTGCGACCA CGCAAGCCCT ATGATCCACC GAGTGAAGCG GCAAAGCAGG AAGTGAGAAA GGTGATGGCC GAAGTTGCGG CGATAGAGGC GGGCTTATCC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA