CoimA.00415.a

Putative uncharacterized protein

CENTER ID: CoimA.00415.a
ORGANISM: Coccidioides immitis RS
ASSOCIATED DISEASE: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00415.a.A1.GE27315 Full length( CoimA.00415.a ) 1 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00415.a.A1.PW27306 Full length( CoimA.00415.a ) 1 471
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CIMG_01371
UniProt: Q1E7U2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASQPQSSSK PAAGKLWGGR FSGATDPLMV AYNESIYFDR AFYAQDIAGS IAFARANVAT GILSQAEFEA IEKGLNQIRG EWENNTFVIQ PGVDEDIHTA NERRLGEIIG KDIAGKLHTG RSRNDQVATD LRLWLRDELK TIETYLVDLI STITDRAEAD IEYLIPGYTH LQRGQPVRWS HWLLSYGTAF LNDLERVREV IKRVNKSPLG CGALSGNAFK IDREAMAKEL GFEGLVMNSM AAVGDRDFIL EAMQCGSTVM LHISRWSEDL ILYSSTEFGF VRLSDAYTTG SSLMPQKKNS DSLELLRGKC GRLFGGMAGL MMTIKGIPST YNKDLQESVE PMIEHVKTLK DSLQIANRVL LTMTTFPDKM IAALTPDMLA TDLAEYLVRK GVPFRETHHI AGRVVALGEK ENKPIDQLTL EQLKSVDQRI GDDVLDVFNY ERSVEMKSAI GGTSKSAVLQ QIKVMRQSLG N
NT Sequence
ATGGCGTCCC AACCACAATC GTCCTCCAAA CCCGCTGCTG GCAAGCTATG GGGCGGCAGA TTCTCCGGAG CAACTGACCC ATTGATGGTG GCATACAATG AGTCCATCTA CTTCGACCGT GCCTTTTACG CTCAAGATAT TGCTGGGTCA ATTGCCTTTG CCCGTGCGAA CGTCGCCACA GGCATCCTCA GCCAAGCTGA ATTCGAAGCC ATCGAGAAGG GTTTGAACCA GATTCGCGGC GAGTGGGAGA ATAACACTTT TGTTATCCAG CCTGGTGTCG ACGAAGATAT TCACACTGCA AACGAGCGCA GACTCGGCGA GATCATTGGG AAGGATATTG CGGGGAAGCT ACATACGGGC AGAAGCCGAA ATGACCAGGT TGCAACAGAC TTGAGACTGT GGTTGAGGGA CGAGTTGAAA ACAATCGAGA CGTATTTGGT CGACTTGATC AGCACCATTA CTGATCGGGC TGAGGCTGAT ATTGAGTACT TGATCCCTGG ATACACCCAC CTTCAGCGTG GACAGCCCGT GAGATGGAGT CATTGGCTGT TGAGCTATGG AACAGCTTTC TTGAACGACC TTGAACGCGT TCGCGAAGTC ATCAAGCGCG TAAACAAGAG TCCGCTGGGC TGTGGTGCAC TCTCGGGAAA CGCATTTAAG ATTGACCGAG AAGCTATGGC TAAAGAGCTT GGTTTCGAAG GGCTAGTCAT GAATAGCATG GCTGCGGTTG GCGACCGGGA CTTTATCCTC GAAGCTATGC AGTGTGGATC AACCGTCATG CTCCACATCT CCCGCTGGAG TGAGGACCTT ATCCTCTACA GTTCCACTGA ATTCGGTTTC GTCCGACTCT CCGACGCTTA CACGACCGGC AGCTCCCTCA TGCCACAAAA GAAAAACAGC GATTCCCTTG AACTGCTCCG CGGTAAATGC GGCAGGCTCT TCGGTGGCAT GGCGGGATTG ATGATGACCA TCAAGGGCAT TCCATCTACA TACAATAAAG ATTTGCAAGA AAGCGTTGAA CCTATGATTG AGCATGTTAA GACGCTCAAA GACAGTCTGC AAATTGCCAA CCGTGTCCTT TTGACCATGA CGACGTTCCC TGATAAAATG ATCGCAGCCC TCACCCCAGA TATGCTAGCT ACCGATTTAG CTGAATACCT TGTCCGGAAA GGCGTCCCAT TTAGAGAAAC ACACCATATT GCTGGCCGTG TCGTCGCATT GGGCGAAAAG GAGAACAAGC CCATTGACCA ACTCACCCTG GAGCAATTGA AATCTGTTGA CCAGCGCATC GGTGATGACG TTCTAGACGT GTTCAACTAT GAACGGTCAG TCGAGATGAA ATCTGCTATT GGCGGTACTA GCAAATCTGC AGTTCTACAG CAGATCAAGG TTATGCGCCA GTCATTAGGT AAC
Details for CoimA.00415.a.A1.GE27315
HARVESTED ON: 10/23/2009
SEQUENCED ON: 10/29/2009
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MASQPQSSSK PAAGKLWGGR FSGATDPLMV AYNESIYFDR AFYAQDIAGS IAFARANVAT GILSQAEFEA IEKGLNQIRG EWENNTFVIQ PGVDEDIHTA NERRLGEIIG KDIAGKLHTG RSRNDQVATD LRLWLRDELK TIETYLVDLI STITDRAEAD IEYLIPGYTH LQRGQPVRWS HWLLSYGTAF LNDLERVREV XKRVNKSPLG CGALSGNAFK IDREAMAKEL GFXGLVMNSM AAVGDRDFIL EAMQCGSTVM LHISRWSEDL ILYSSTEFGF VRLSDAYTTG SSLMPQKKNS DSLELXRGKC GRLFGGMAGL MMTIKGIPST YNKDLQESVE PMIEHVKTLK DSLQIANRVL LTMTTFPDKM IAALTPDMLA TDLAEYLVRK GVPFRETHHI AGRVVALGEK ENKPIDQLTL EQLKSVDQRI GDDVLDVFNY ERSVEMKSAI GGTSKSAVLQ QIKVMRQSLG N
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatggcgtc ccaaccacaa tcgtcctcca aacccgctgc tggcaagcta tggggcggca gattctccgg agcaactgac ccattgatgg tggcatacaa tgagtccatc tacttcgacc gtgcctttta cgctcaagat attgctgggt caattgcctt tgcccgtgcg aacgtcgcca caggcatcct cagccaagct gaattcgaag ccatcgagaa gggtttgaac cagattcgcg gcgagtggga gaataacact tttgttatcc agcctggtgt cgacgaagat attcacactg caaacgagcg cagactcggc gagatcattg ggaaggatat tgcggggaag ctacatacgg gcagaagccg aaatgaccag gttgcaacag acttgagact gtggttgagg gacgagttga aaacaatcga gacgtatttg gtcgacttga tcagcaccat tactgatcgg gctgaggctg atattgagta cttgatccct ggatacaccc accttcagcg tggacagccc gtgagatgga gtcattggct gttgagctat ggaacagctt tcttgaacga ccttgaacgc gttcgcgaag tcntcaagcg cgtaaacaag agtccgctgg gctgtggtgc actctcggga aacgcattta agattgaccg agaagctatg gctaaagagc ttggtttcna agggctagtc atgaatagca tggctgcggt tggcgaccgg gactttatcc tcgaagctat gcagtgtgga tcaaccgtca tgctccacat ctcccgctgg agtgaggacc ttatcctcta cagttccact gaattcggtt tcgtccgact ctccgacgct tacacgaccg gcagctccct catgccacaa aagaaaaaca gcgattccct tgaactgntc cgcggtaaat gcggcaggct cttcggtggc atggcgggat tgatgatgac catcaagggc attccatcta catacaataa agatttgcaa gaaagcgttg aacctatgat tgagcatgtt aagacgctca aagacagtct gcaaattgcc aaccgtgtcc ttttgaccat gacgacgttc cctgataaaa tgatcgcagc cctcacccca gatatgctag ctaccgattt agctgaatac cttgtccgga aaggcgtccc atttagagaa acacaccata ttgctggccg tgtcgtcgca ttgggcgaaa aggagaacaa gcccattgac caactcaccc tggagcaatt gaaatctgtt gaccagcgca tcggtgatga cgttctagac gtgttcaact atgaacggtc agtcgagatg aaatctgcta ttggcggtac tagcaaatct gcagttctac agcagatcaa ggttatgcgc cagtcattag gtaactaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASQPQSSS KPAAGKLWGG RFSGATDPLM VAYNESIYFD RAFYAQDIAG SIAFARANVA TGILSQAEFE AIEKGLNQIR GEWENNTFVI QPGVDEDIHT ANERRLGEII GKDIAGKLHT GRSRNDQVAT DLRLWLRDEL KTIETYLVDL ISTITDRAEA DIEYLIPGYT HLQRGQPVRW SHWLLSYGTA FLNDLERVRE VIKRVNKSPL GCGALSGNAF KIDREAMAKE LGFEGLVMNS MAAVGDRDFI LEAMQCGSTV MLHISRWSED LILYSSTEFG FVRLSDAYTT GSSLMPQKKN SDSLELLRGK CGRLFGGMAG LMMTIKGIPS TYNKDLQESV EPMIEHVKTL KDSLQIANRV LLTMTTFPDK MIAALTPDML ATDLAEYLVR KGVPFRETHH IAGRVVALGE KENKPIDQLT LEQLKSVDQR IGDDVLDVFN YERSVEMKSA IGGTSKSAVL QQIKVMRQSL GN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtcccaa ccacaatcgt cctccaaacc cgctgctggc aagctatggg gcggcagatt ctccggagca actgacccat tgatggtggc atacaatgag tccatctact tcgaccgtgc cttttacgct caagatattg ctgggtcaat tgcctttgcc cgtgcgaacg tcgccacagg catcctcagc caagctgaat tcgaagccat cgagaagggt ttgaaccaga ttcgcggcga gtgggagaat aacacttttg ttatccagcc tggtgtcgac gaagatattc acactgcaaa cgagcgcaga ctcggcgaga tcattgggaa ggatattgcg gggaagctac atacgggcag aagccgaaat gaccaggttg caacagactt gagactgtgg ttgagggacg agttgaaaac aatcgagacg tatttggtcg acttgatcag caccattact gatcgggctg aggctgatat tgagtacttg atccctggat acacccacct tcagcgtgga cagcccgtga gatggagtca ttggctgttg agctatggaa cagctttctt gaacgacctt gaacgcgttc gcgaagtcat caagcgcgta aacaagagtc cgctgggctg tggtgcactc tcgggaaacg catttaagat tgaccgagaa gctatggcta aagagcttgg tttcgaaggg ctagtcatga atagcatggc tgcggttggc gaccgggact ttatcctcga agctatgcag tgtggatcaa ccgtcatgct ccacatctcc cgctggagtg aggaccttat cctctacagt tccactgaat tcggtttcgt ccgactctcc gacgcttaca cgaccggcag ctccctcatg ccacaaaaga aaaacagcga ttcccttgaa ctgctccgcg gtaaatgcgg caggctcttc ggtggcatgg cgggattgat gatgaccatc aagggcattc catctacata caataaagat ttgcaagaaa gcgttgaacc tatgattgag catgttaaga cgctcaaaga cagtctgcaa attgccaacc gtgtcctttt gaccatgacg acgttccctg ataaaatgat cgcagccctc accccagata tgctagctac cgatttagct gaataccttg tccggaaagg cgtcccattt agagaaacac accatattgc tggccgtgtc gtcgcattgg gcgaaaagga gaacaagccc attgaccaac tcaccctgga gcaattgaaa tctgttgacc agcgcatcgg tgatgacgtt ctagacgtgt tcaactatga acggtcagtc gagatgaaat ctgctattgg cggtactagc aaatctgcag ttctacagca gatcaaggtt atgcgccagt cattaggtaa caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for CoimA.00415.a.A1.PW27306
PURIFICATION DATe: 12/7/2009
CONCENTRATION: 29.04mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 25
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MASQPQSSSK PAAGKLWGGR FSGATDPLMV AYNESIYFDR AFYAQDIAGS IAFARANVAT GILSQAEFEA IEKGLNQIRG EWENNTFVIQ PGVDEDIHTA NERRLGEIIG KDIAGKLHTG RSRNDQVATD LRLWLRDELK TIETYLVDLI STITDRAEAD IEYLIPGYTH LQRGQPVRWS HWLLSYGTAF LNDLERVREV XKRVNKSPLG CGALSGNAFK IDREAMAKEL GFXGLVMNSM AAVGDRDFIL EAMQCGSTVM LHISRWSEDL ILYSSTEFGF VRLSDAYTTG SSLMPQKKNS DSLELXRGKC GRLFGGMAGL MMTIKGIPST YNKDLQESVE PMIEHVKTLK DSLQIANRVL LTMTTFPDKM IAALTPDMLA TDLAEYLVRK GVPFRETHHI AGRVVALGEK ENKPIDQLTL EQLKSVDQRI GDDVLDVFNY ERSVEMKSAI GGTSKSAVLQ QIKVMRQSLG N
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatggcgtc ccaaccacaa tcgtcctcca aacccgctgc tggcaagcta tggggcggca gattctccgg agcaactgac ccattgatgg tggcatacaa tgagtccatc tacttcgacc gtgcctttta cgctcaagat attgctgggt caattgcctt tgcccgtgcg aacgtcgcca caggcatcct cagccaagct gaattcgaag ccatcgagaa gggtttgaac cagattcgcg gcgagtggga gaataacact tttgttatcc agcctggtgt cgacgaagat attcacactg caaacgagcg cagactcggc gagatcattg ggaaggatat tgcggggaag ctacatacgg gcagaagccg aaatgaccag gttgcaacag acttgagact gtggttgagg gacgagttga aaacaatcga gacgtatttg gtcgacttga tcagcaccat tactgatcgg gctgaggctg atattgagta cttgatccct ggatacaccc accttcagcg tggacagccc gtgagatgga gtcattggct gttgagctat ggaacagctt tcttgaacga ccttgaacgc gttcgcgaag tcntcaagcg cgtaaacaag agtccgctgg gctgtggtgc actctcggga aacgcattta agattgaccg agaagctatg gctaaagagc ttggtttcna agggctagtc atgaatagca tggctgcggt tggcgaccgg gactttatcc tcgaagctat gcagtgtgga tcaaccgtca tgctccacat ctcccgctgg agtgaggacc ttatcctcta cagttccact gaattcggtt tcgtccgact ctccgacgct tacacgaccg gcagctccct catgccacaa aagaaaaaca gcgattccct tgaactgntc cgcggtaaat gcggcaggct cttcggtggc atggcgggat tgatgatgac catcaagggc attccatcta catacaataa agatttgcaa gaaagcgttg aacctatgat tgagcatgtt aagacgctca aagacagtct gcaaattgcc aaccgtgtcc ttttgaccat gacgacgttc cctgataaaa tgatcgcagc cctcacccca gatatgctag ctaccgattt agctgaatac cttgtccgga aaggcgtccc atttagagaa acacaccata ttgctggccg tgtcgtcgca ttgggcgaaa aggagaacaa gcccattgac caactcaccc tggagcaatt gaaatctgtt gaccagcgca tcggtgatga cgttctagac gtgttcaact atgaacggtc agtcgagatg aaatctgcta ttggcggtac tagcaaatct gcagttctac agcagatcaa ggttatgcgc cagtcattag gtaactaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASQPQSSS KPAAGKLWGG RFSGATDPLM VAYNESIYFD RAFYAQDIAG SIAFARANVA TGILSQAEFE AIEKGLNQIR GEWENNTFVI QPGVDEDIHT ANERRLGEII GKDIAGKLHT GRSRNDQVAT DLRLWLRDEL KTIETYLVDL ISTITDRAEA DIEYLIPGYT HLQRGQPVRW SHWLLSYGTA FLNDLERVRE VIKRVNKSPL GCGALSGNAF KIDREAMAKE LGFEGLVMNS MAAVGDRDFI LEAMQCGSTV MLHISRWSED LILYSSTEFG FVRLSDAYTT GSSLMPQKKN SDSLELLRGK CGRLFGGMAG LMMTIKGIPS TYNKDLQESV EPMIEHVKTL KDSLQIANRV LLTMTTFPDK MIAALTPDML ATDLAEYLVR KGVPFRETHH IAGRVVALGE KENKPIDQLT LEQLKSVDQR IGDDVLDVFN YERSVEMKSA IGGTSKSAVL QQIKVMRQSL GN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTCCCAA CCACAATCGT CCTCCAAACC CGCTGCTGGC AAGCTATGGG GCGGCAGATT CTCCGGAGCA ACTGACCCAT TGATGGTGGC ATACAATGAG TCCATCTACT TCGACCGTGC CTTTTACGCT CAAGATATTG CTGGGTCAAT TGCCTTTGCC CGTGCGAACG TCGCCACAGG CATCCTCAGC CAAGCTGAAT TCGAAGCCAT CGAGAAGGGT TTGAACCAGA TTCGCGGCGA GTGGGAGAAT AACACTTTTG TTATCCAGCC TGGTGTCGAC GAAGATATTC ACACTGCAAA CGAGCGCAGA CTCGGCGAGA TCATTGGGAA GGATATTGCG GGGAAGCTAC ATACGGGCAG AAGCCGAAAT GACCAGGTTG CAACAGACTT GAGACTGTGG TTGAGGGACG AGTTGAAAAC AATCGAGACG TATTTGGTCG ACTTGATCAG CACCATTACT GATCGGGCTG AGGCTGATAT TGAGTACTTG ATCCCTGGAT ACACCCACCT TCAGCGTGGA CAGCCCGTGA GATGGAGTCA TTGGCTGTTG AGCTATGGAA CAGCTTTCTT GAACGACCTT GAACGCGTTC GCGAAGTCAT CAAGCGCGTA AACAAGAGTC CGCTGGGCTG TGGTGCACTC TCGGGAAACG CATTTAAGAT TGACCGAGAA GCTATGGCTA AAGAGCTTGG TTTCGAAGGG CTAGTCATGA ATAGCATGGC TGCGGTTGGC GACCGGGACT TTATCCTCGA AGCTATGCAG TGTGGATCAA CCGTCATGCT CCACATCTCC CGCTGGAGTG AGGACCTTAT CCTCTACAGT TCCACTGAAT TCGGTTTCGT CCGACTCTCC GACGCTTACA CGACCGGCAG CTCCCTCATG CCACAAAAGA AAAACAGCGA TTCCCTTGAA CTGCTCCGCG GTAAATGCGG CAGGCTCTTC GGTGGCATGG CGGGATTGAT GATGACCATC AAGGGCATTC CATCTACATA CAATAAAGAT TTGCAAGAAA GCGTTGAACC TATGATTGAG CATGTTAAGA CGCTCAAAGA CAGTCTGCAA ATTGCCAACC GTGTCCTTTT GACCATGACG ACGTTCCCTG ATAAAATGAT CGCAGCCCTC ACCCCAGATA TGCTAGCTAC CGATTTAGCT GAATACCTTG TCCGGAAAGG CGTCCCATTT AGAGAAACAC ACCATATTGC TGGCCGTGTC GTCGCATTGG GCGAAAAGGA GAACAAGCCC ATTGACCAAC TCACCCTGGA GCAATTGAAA TCTGTTGACC AGCGCATCGG TGATGACGTT CTAGACGTGT TCAACTATGA ACGGTCAGTC GAGATGAAAT CTGCTATTGG CGGTACTAGC AAATCTGCAG TTCTACAGCA GATCAAGGTT ATGCGCCAGT CATTAGGTAA CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA