CoimA.00410.a
Putative glutathione transferase
| CENTER ID: | CoimA.00410.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00410.a.A1.GE27314 | Full length( CoimA.00410.a ) | 1 | 231 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00410.a.A1.PW27305 | Full length( CoimA.00410.a ) | 1 | 231 |
Structures
3LG6
DEPOSITED: 1/19/2010
DETERMINATION: XRay
CLONE: CoimA.00410.a.A1.GE27314
PROTEIN: CoimA.00410.a.A1.PW27305
3N5O
DEPOSITED: 5/25/2010
DETERMINATION: XRay
CLONE: CoimA.00410.a.A1.GE27314
PROTEIN: CoimA.00410.a.A1.PW27305
Publications by SSGCID
Structures of a putative ΞΆ-class glutathione S-transferase from the pathogenic fungus Coccidioides immitis.
Abendroth J, Bryan CM, Dieterich SH, Edwards TE, Leibly DJ, Myler PJ, Sankaran B, Sivam D, Staker BL, Stewart LJ, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1038-43
PMID: 21904047; PMCID: PMC3169399
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_01314 |
| OrthoMCL: | OG5_129965 |
| RefSeq: | XP_001247543.1 |
| UniProt: | D2YW48 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTPNFELYG YFRSSCSGRL RIAFHLKSIP YTRHPVNLLK GEQHSDTYKS LNPTNTVPLL VVSNINNTVS PSSASFSIGQ SLAALEYLEE ALPTNARPLL PPISNPVARA HVRTICNIIA CDVQPVTNLK IQKKVKALDG DPTVWSRDLA TQGFGAVEKL LELSAGRFCV GDEITLADVC LVPAVWAAER VGMDLARFPI TKRVFEEMLK EEAVQKAHWQ KQEDTPEDLR A
NT Sequence
ATGACGACTC CCAACTTCGA ACTCTACGGC TACTTCCGCT CCTCCTGCTC CGGCCGCCTG CGCATCGCCT TCCACCTCAA ATCCATCCCC TACACTCGCC ACCCCGTCAA CCTCCTCAAG GGCGAACAGC ACTCCGACAC CTACAAGTCC CTCAATCCAA CCAACACCGT GCCCCTCCTC GTCGTCTCCA ACATCAACAA CACCGTCTCC CCATCCTCCG CCTCCTTCTC CATCGGCCAA TCCCTCGCCG CACTCGAATA CCTCGAAGAA GCCCTCCCCA CCAACGCGCG CCCGCTCCTC CCGCCCATCT CCAACCCCGT TGCCCGCGCC CACGTCCGCA CCATCTGCAA CATCATCGCC TGCGACGTCC AGCCCGTCAC AAACCTCAAG ATCCAGAAGA AAGTCAAGGC CTTGGACGGC GACCCGACTG TGTGGTCGCG CGATCTCGCC ACGCAGGGGT TCGGTGCGGT GGAGAAGTTG CTCGAGCTCA GTGCGGGGCG ATTCTGCGTC GGTGACGAGA TCACCTTGGC CGATGTGTGT CTAGTTCCCG CCGTGTGGGC GGCGGAGCGA GTGGGGATGG ACCTGGCCCG GTTTCCTATC ACCAAGAGGG TGTTTGAAGA GATGCTGAAG GAGGAGGCCG TGCAGAAGGC GCATTGGCAG AAGCAGGAGG ATACCCCGGA GGATTTGAGG GCT
Details for CoimA.00410.a.A1.GE27314
| HARVESTED ON: | 10/23/2009 |
| SEQUENCED ON: | 10/29/2009 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTPNFELY GYFRSSCSGR LRIAFHLKSI PYTRHPVNLL KGEQHSDTYK SLNPTNTVPL LVVSNINNTV SPSSASFSIG QSLAALEYLE EALPTNARPL LPPISNPVAR AHVRTICNII ACDVQPVTNL KIQKKVKALD GDPTVWSRDL ATQGFGAVEK LLELSAGRFC VGDEITLADV CLVPAVWAAE RVGMDLARFP ITKRVFEEML KEEAVQKAHW QKQEDTPEDL RA
Validated NT Sequence
ttattaagcc ctcaaatcct ccggggtatc ctcctgcttc tgccaatgcg ccttctgcac ggcctcctcc ttcagcatct cttcaaacac cctcttggtg ataggaaacc gggccaggtc catccccact cgctccgccg cccacacggc gggaactaga cacacatcgg ccaaggtgat ctcgtcaccg acgcagaatc gccccgcact gagctcgagc aacttctcca ccgcaccgaa cccctgcgtg gcgagatcgc gcgaccacac agtcgggtcg ccgtccaagg ccttgacttt cttctggatc ttgaggtttg tgacgggctg gacgtcgcag gcgatgatgt tgcagatggt gcggacgtgg gcgcgggcaa cggggttgga gatgggcggg aggagcgggc gcgcgttggt ggggagggct tcttcgaggt attcgagtgc ggcgagggat tggccgatgg agaaggaggc ggaggatggg gagacggtgt tgttgatgtt ggagacgacg aggaggggca cggtgttggt tggattgagg gacttgtagg tgtcggagtg ctgttcgccc ttgaggaggt tgacggggtg gcgagtgtag gggatggatt tgaggtggaa ggcgatgcgc aggcggccgg agcaggagga gcggaagtag ccgtagagtt cgaagttggg agtcgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTPNFELY GYFRSSCSGR LRIAFHLKSI PYTRHPVNLL KGEQHSDTYK SLNPTNTVPL LVVSNINNTV SPSSASFSIG QSLAALEYLE EALPTNARPL LPPISNPVAR AHVRTICNII ACDVQPVTNL KIQKKVKALD GDPTVWSRDL ATQGFGAVEK LLELSAGRFC VGDEITLADV CLVPAVWAAE RVGMDLARFP ITKRVFEEML KEEAVQKAHW QKQEDTPEDL RA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgactccc aacttcgaac tctacggcta cttccgctcc tcctgctccg gccgcctgcg catcgccttc cacctcaaat ccatccccta cactcgccac cccgtcaacc tcctcaaggg cgaacagcac tccgacacct acaagtccct caatccaacc aacaccgtgc ccctcctcgt cgtctccaac atcaacaaca ccgtctcccc atcctccgcc tccttctcca tcggccaatc cctcgccgca ctcgaatacc tcgaagaagc cctccccacc aacgcgcgcc cgctcctccc gcccatctcc aaccccgttg cccgcgccca cgtccgcacc atctgcaaca tcatcgcctg cgacgtccag cccgtcacaa acctcaagat ccagaagaaa gtcaaggcct tggacggcga cccgactgtg tggtcgcgcg atctcgccac gcaggggttc ggtgcggtgg agaagttgct cgagctcagt gcggggcgat tctgcgtcgg tgacgagatc accttggccg atgtgtgtct agttcccgcc gtgtgggcgg cggagcgagt ggggatggac ctggcccggt ttcctatcac caagagggtg tttgaagaga tgctgaagga ggaggccgtg cagaaggcgc attggcagaa gcaggaggat accccggagg atttgagggc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for CoimA.00410.a.A1.PW27305
| PURIFICATION DATe: | 12/7/2009 |
| CONCENTRATION: | 23.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTPNFELY GYFRSSCSGR LRIAFHLKSI PYTRHPVNLL KGEQHSDTYK SLNPTNTVPL LVVSNINNTV SPSSASFSIG QSLAALEYLE EALPTNARPL LPPISNPVAR AHVRTICNII ACDVQPVTNL KIQKKVKALD GDPTVWSRDL ATQGFGAVEK LLELSAGRFC VGDEITLADV CLVPAVWAAE RVGMDLARFP ITKRVFEEML KEEAVQKAHW QKQEDTPEDL RA
Validated NT Sequence
ttattaagcc ctcaaatcct ccggggtatc ctcctgcttc tgccaatgcg ccttctgcac ggcctcctcc ttcagcatct cttcaaacac cctcttggtg ataggaaacc gggccaggtc catccccact cgctccgccg cccacacggc gggaactaga cacacatcgg ccaaggtgat ctcgtcaccg acgcagaatc gccccgcact gagctcgagc aacttctcca ccgcaccgaa cccctgcgtg gcgagatcgc gcgaccacac agtcgggtcg ccgtccaagg ccttgacttt cttctggatc ttgaggtttg tgacgggctg gacgtcgcag gcgatgatgt tgcagatggt gcggacgtgg gcgcgggcaa cggggttgga gatgggcggg aggagcgggc gcgcgttggt ggggagggct tcttcgaggt attcgagtgc ggcgagggat tggccgatgg agaaggaggc ggaggatggg gagacggtgt tgttgatgtt ggagacgacg aggaggggca cggtgttggt tggattgagg gacttgtagg tgtcggagtg ctgttcgccc ttgaggaggt tgacggggtg gcgagtgtag gggatggatt tgaggtggaa ggcgatgcgc aggcggccgg agcaggagga gcggaagtag ccgtagagtt cgaagttggg agtcgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTPNFELY GYFRSSCSGR LRIAFHLKSI PYTRHPVNLL KGEQHSDTYK SLNPTNTVPL LVVSNINNTV SPSSASFSIG QSLAALEYLE EALPTNARPL LPPISNPVAR AHVRTICNII ACDVQPVTNL KIQKKVKALD GDPTVWSRDL ATQGFGAVEK LLELSAGRFC VGDEITLADV CLVPAVWAAE RVGMDLARFP ITKRVFEEML KEEAVQKAHW QKQEDTPEDL RA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGACTCCC AACTTCGAAC TCTACGGCTA CTTCCGCTCC TCCTGCTCCG GCCGCCTGCG CATCGCCTTC CACCTCAAAT CCATCCCCTA CACTCGCCAC CCCGTCAACC TCCTCAAGGG CGAACAGCAC TCCGACACCT ACAAGTCCCT CAATCCAACC AACACCGTGC CCCTCCTCGT CGTCTCCAAC ATCAACAACA CCGTCTCCCC ATCCTCCGCC TCCTTCTCCA TCGGCCAATC CCTCGCCGCA CTCGAATACC TCGAAGAAGC CCTCCCCACC AACGCGCGCC CGCTCCTCCC GCCCATCTCC AACCCCGTTG CCCGCGCCCA CGTCCGCACC ATCTGCAACA TCATCGCCTG CGACGTCCAG CCCGTCACAA ACCTCAAGAT CCAGAAGAAA GTCAAGGCCT TGGACGGCGA CCCGACTGTG TGGTCGCGCG ATCTCGCCAC GCAGGGGTTC GGTGCGGTGG AGAAGTTGCT CGAGCTCAGT GCGGGGCGAT TCTGCGTCGG TGACGAGATC ACCTTGGCCG ATGTGTGTCT AGTTCCCGCC GTGTGGGCGG CGGAGCGAGT GGGGATGGAC CTGGCCCGGT TTCCTATCAC CAAGAGGGTG TTTGAAGAGA TGCTGAAGGA GGAGGCCGTG CAGAAGGCGC ATTGGCAGAA GCAGGAGGAT ACCCCGGAGG ATTTGAGGGC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA