CoimA.00345.a
Putative fructose-1,6-biphosphate aldolase
| CENTER ID: | CoimA.00345.a |
| ORGANISM: | Coccidioides immitis RS |
| ASSOCIATED DISEASE: | California disease, Desert rheumatism, San Joaquin valley fever, Valley fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00345.a.A1.GE27865 | Full length( CoimA.00345.a ) | 1 | 302 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CoimA.00345.a.A1.PS00465 | Full length( CoimA.00345.a ) | 1 | 302 |
Structures
3PM6
DEPOSITED: 11/16/2010
DETERMINATION: XRay
CLONE: CoimA.00345.a.A1.GE27865
PROTEIN: CoimA.00345.a.A1.PS00465
Publications by SSGCID
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PMID: 21359836; PMCID: PMC3123459
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CIMG_05755 |
| UniProt: | Q1DVA8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPHPSLKSNR ALPLLTFART HSFAIPAICV YNLEGILAII RAAEHKRSPA MILLFPWAIQ YADSLLVRTA ASACRAASVP ITLHLDHAQD PEIIKRAADL SRSETHEPGF DSIMVDMSHF SKEENLRLTR ELVAYCNARG IATEAEPGRI EGGEDGVQDT VDLEGVLTTP EESEEFVATG INWLAPAFGN VHGNYGPRGV QLDYERLQRI NEAVGERVGL VLHGADPFTK EIFEKCIERG VAKVNVNRAV NNEYVKVMRE KAGSLPITRL HEEVTNAMQA AVEKIMDMID STGKAEFMMD EK
NT Sequence
ATGCCTCACC CATCTCTCAA ATCCAACCGT GCCCTTCCTC TCCTTACGTT CGCACGTACC CACTCCTTCG CCATCCCCGC AATATGCGTC TACAACCTTG AAGGCATCCT AGCCATCATC CGTGCTGCCG AACACAAACG CTCCCCAGCC ATGATCCTTC TCTTCCCGTG GGCCATCCAG TATGCCGACT CACTCCTCGT CCGCACCGCC GCATCTGCAT GTCGAGCAGC CAGCGTGCCC ATCACACTAC ACCTAGACCA CGCCCAAGAC CCAGAAATTA TCAAGCGGGC CGCAGACCTG TCGCGATCGG AAACCCACGA ACCGGGCTTC GATAGTATCA TGGTTGACAT GAGCCATTTC TCCAAGGAGG AGAATCTGCG ACTGACGCGT GAGCTGGTGG CATATTGCAA TGCTCGTGGG ATCGCGACGG AGGCGGAGCC GGGAAGAATC GAAGGCGGAG AGGATGGCGT GCAGGATACG GTGGATTTGG AAGGTGTCTT GACGACCCCG GAGGAGAGCG AGGAGTTTGT GGCGACGGGG ATTAACTGGT TGGCTCCGGC GTTTGGGAAT GTTCACGGCA ATTATGGGCC GAGGGGCGTG CAGCTGGATT ATGAGCGGTT GCAGAGGATT AATGAGGCGG TGGGGGAACG GGTGGGACTT GTGTTGCACG GCGCTGACCC ATTTACGAAG GAGATCTTTG AGAAGTGTAT TGAGCGCGGG GTGGCTAAGG TTAATGTTAA TAGGGCCGTG AATAATGAGT ATGTGAAAGT GATGAGGGAG AAAGCAGGGA GTTTGCCTAT TACAAGGCTG CATGAGGAGG TCACAAATGC CATGCAGGCT GCAGTCGAGA AGATCATGGA TATGATTGAT TCCACAGGAA AGGCTGAATT TATGATGGAC GAAAAA
Details for CoimA.00345.a.A1.GE27865
| HARVESTED ON: | 11/3/2009 |
| SEQUENCED ON: | 12/10/2009 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPHPSLKSN RALPLLTFAR THSFAIPAIC VYNLEGILAI IRAAEHKRSP AMILLFPWAI QYADSLLVRT AASACRAASV PITLHLDHAQ DPEIIKRAAD LSRSETHEPG FDSIMVDMSH FSKEENLRLT RELVAYCNAR GIATEAEPGR IEGGEDGVQD TVDLEGVLTT PEESEEFVAT GINWLAPAFG NVHGNYGPRG VQLDYERLQR INEAVGERVG LVLHGADPFT KEIFEKCIER GVAKVNVNRA VNNEYVKVMR EKAGSLPITR LHEEVTNAMQ AAVEKIMDMI DSTGKAEFMM D
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctc acccatctct caaatccaac cgtgcccttc ctctccttac gttcgcacgt acccactcct tcgccatccc cgcaatatgc gtctacaacc ttgaaggcat cctagccatc atccgtgctg ccgaacacaa acgctcccca gccatgatcc ttctcttccc gtgggccatc cagtatgccg actcactcct cgtccgcacc gccgcatctg catgtcgagc agccagcgtg cccatcacac tacacctaga ccacgcccaa gacccagaaa ttatcaagcg ggccgcagac ctgtcgcgat cggaaaccca cgaaccgggc ttcgatagta tcatggttga catgagccat ttctccaagg aggagaatct gcgactgacg cgtgagctgg tggcatattg caatgctcgt gggatcgcga cggaggcgga gccgggaaga atcgaaggcg gagaggatgg cgtgcaggat acggtggatt tggaaggtgt cttgacgacc ccggaggaga gcgaggagtt tgtggcgacg gggattaact ggttggctcc ggcgtttggg aatgttcacg gcaattatgg gccgaggggc gtgcagctgg attatgagcg gttgcagagg attaatgagg cggtggggga acgggtggga cttgtgttgc acggcgctga cccatttacg aaggagatct ttgagaagtg tattgagcgc ggggtggcta aggttaatgt taatagggcc gtgaataatg agtatgtgaa agtgatgagg gagaaagcag ggagtttgcc tattacaagg ctgcatgagg aggtcacaaa tgccatgcag gctgcagtcg agaagatcat ggatatgatt gattccacag gaaaggctga atttatgatg gacg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPHPSLKSN RALPLLTFAR THSFAIPAIC VYNLEGILAI IRAAEHKRSP AMILLFPWAI QYADSLLVRT AASACRAASV PITLHLDHAQ DPEIIKRAAD LSRSETHEPG FDSIMVDMSH FSKEENLRLT RELVAYCNAR GIATEAEPGR IEGGEDGVQD TVDLEGVLTT PEESEEFVAT GINWLAPAFG NVHGNYGPRG VQLDYERLQR INEAVGERVG LVLHGADPFT KEIFEKCIER GVAKVNVNRA VNNEYVKVMR EKAGSLPITR LHEEVTNAMQ AAVEKIMDMI DSTGKAEFMM DEK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctcaccca tctctcaaat ccaaccgtgc ccttcctctc cttacgttcg cacgtaccca ctccttcgcc atccccgcaa tatgcgtcta caaccttgaa ggcatcctag ccatcatccg tgctgccgaa cacaaacgct ccccagccat gatccttctc ttcccgtggg ccatccagta tgccgactca ctcctcgtcc gcaccgccgc atctgcatgt cgagcagcca gcgtgcccat cacactacac ctagaccacg cccaagaccc agaaattatc aagcgggccg cagacctgtc gcgatcggaa acccacgaac cgggcttcga tagtatcatg gttgacatga gccatttctc caaggaggag aatctgcgac tgacgcgtga gctggtggca tattgcaatg ctcgtgggat cgcgacggag gcggagccgg gaagaatcga aggcggagag gatggcgtgc aggatacggt ggatttggaa ggtgtcttga cgaccccgga ggagagcgag gagtttgtgg cgacggggat taactggttg gctccggcgt ttgggaatgt tcacggcaat tatgggccga ggggcgtgca gctggattat gagcggttgc agaggattaa tgaggcggtg ggggaacggg tgggacttgt gttgcacggc gctgacccat ttacgaagga gatctttgag aagtgtattg agcgcggggt ggctaaggtt aatgttaata gggccgtgaa taatgagtat gtgaaagtga tgagggagaa agcagggagt ttgcctatta caaggctgca tgaggaggtc acaaatgcca tgcaggctgc agtcgagaag atcatggata tgattgattc cacaggaaag gctgaattta tgatggacga aaaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for CoimA.00345.a.A1.PS00465
| PURIFICATION DATe: | 1/28/2010 |
| CONCENTRATION: | 23.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPHPSLKSN RALPLLTFAR THSFAIPAIC VYNLEGILAI IRAAEHKRSP AMILLFPWAI QYADSLLVRT AASACRAASV PITLHLDHAQ DPEIIKRAAD LSRSETHEPG FDSIMVDMSH FSKEENLRLT RELVAYCNAR GIATEAEPGR IEGGEDGVQD TVDLEGVLTT PEESEEFVAT GINWLAPAFG NVHGNYGPRG VQLDYERLQR INEAVGERVG LVLHGADPFT KEIFEKCIER GVAKVNVNRA VNNEYVKVMR EKAGSLPITR LHEEVTNAMQ AAVEKIMDMI DSTGKAEFMM D
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctc acccatctct caaatccaac cgtgcccttc ctctccttac gttcgcacgt acccactcct tcgccatccc cgcaatatgc gtctacaacc ttgaaggcat cctagccatc atccgtgctg ccgaacacaa acgctcccca gccatgatcc ttctcttccc gtgggccatc cagtatgccg actcactcct cgtccgcacc gccgcatctg catgtcgagc agccagcgtg cccatcacac tacacctaga ccacgcccaa gacccagaaa ttatcaagcg ggccgcagac ctgtcgcgat cggaaaccca cgaaccgggc ttcgatagta tcatggttga catgagccat ttctccaagg aggagaatct gcgactgacg cgtgagctgg tggcatattg caatgctcgt gggatcgcga cggaggcgga gccgggaaga atcgaaggcg gagaggatgg cgtgcaggat acggtggatt tggaaggtgt cttgacgacc ccggaggaga gcgaggagtt tgtggcgacg gggattaact ggttggctcc ggcgtttggg aatgttcacg gcaattatgg gccgaggggc gtgcagctgg attatgagcg gttgcagagg attaatgagg cggtggggga acgggtggga cttgtgttgc acggcgctga cccatttacg aaggagatct ttgagaagtg tattgagcgc ggggtggcta aggttaatgt taatagggcc gtgaataatg agtatgtgaa agtgatgagg gagaaagcag ggagtttgcc tattacaagg ctgcatgagg aggtcacaaa tgccatgcag gctgcagtcg agaagatcat ggatatgatt gattccacag gaaaggctga atttatgatg gacg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPHPSLKSN RALPLLTFAR THSFAIPAIC VYNLEGILAI IRAAEHKRSP AMILLFPWAI QYADSLLVRT AASACRAASV PITLHLDHAQ DPEIIKRAAD LSRSETHEPG FDSIMVDMSH FSKEENLRLT RELVAYCNAR GIATEAEPGR IEGGEDGVQD TVDLEGVLTT PEESEEFVAT GINWLAPAFG NVHGNYGPRG VQLDYERLQR INEAVGERVG LVLHGADPFT KEIFEKCIER GVAKVNVNRA VNNEYVKVMR EKAGSLPITR LHEEVTNAMQ AAVEKIMDMI DSTGKAEFMM DEK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTCACCCA TCTCTCAAAT CCAACCGTGC CCTTCCTCTC CTTACGTTCG CACGTACCCA CTCCTTCGCC ATCCCCGCAA TATGCGTCTA CAACCTTGAA GGCATCCTAG CCATCATCCG TGCTGCCGAA CACAAACGCT CCCCAGCCAT GATCCTTCTC TTCCCGTGGG CCATCCAGTA TGCCGACTCA CTCCTCGTCC GCACCGCCGC ATCTGCATGT CGAGCAGCCA GCGTGCCCAT CACACTACAC CTAGACCACG CCCAAGACCC AGAAATTATC AAGCGGGCCG CAGACCTGTC GCGATCGGAA ACCCACGAAC CGGGCTTCGA TAGTATCATG GTTGACATGA GCCATTTCTC CAAGGAGGAG AATCTGCGAC TGACGCGTGA GCTGGTGGCA TATTGCAATG CTCGTGGGAT CGCGACGGAG GCGGAGCCGG GAAGAATCGA AGGCGGAGAG GATGGCGTGC AGGATACGGT GGATTTGGAA GGTGTCTTGA CGACCCCGGA GGAGAGCGAG GAGTTTGTGG CGACGGGGAT TAACTGGTTG GCTCCGGCGT TTGGGAATGT TCACGGCAAT TATGGGCCGA GGGGCGTGCA GCTGGATTAT GAGCGGTTGC AGAGGATTAA TGAGGCGGTG GGGGAACGGG TGGGACTTGT GTTGCACGGC GCTGACCCAT TTACGAAGGA GATCTTTGAG AAGTGTATTG AGCGCGGGGT GGCTAAGGTT AATGTTAATA GGGCCGTGAA TAATGAGTAT GTGAAAGTGA TGAGGGAGAA AGCAGGGAGT TTGCCTATTA CAAGGCTGCA TGAGGAGGTC ACAAATGCCA TGCAGGCTGC AGTCGAGAAG ATCATGGATA TGATTGATTC CACAGGAAAG GCTGAATTTA TGATGGACGA AAAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA