CoimA.00226.a

Putative uncharacterized protein

CENTER ID: CoimA.00226.a
ORGANISM: Coccidioides immitis RS
ASSOCIATED DISEASE: California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00226.a.A1.GE27312 Full length( CoimA.00226.a ) 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CoimA.00226.a.A1.PW27303 Full length( CoimA.00226.a ) 1 350

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CIMG_02231
UniProt: Q1E5D2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTALPEPFAS IPRKALLLEP SPIHLLPNMT ADLGGAVKIY AKRDDLNSGL AYGGNKTRKL EYLVADALAT KCDTLISIGG IQSNHTRQVA AAAAHAGLKA KLVQEKWVEW SDPGYESVGN IQLSRLMGAD VRIERMAGFG IEHKDSLKNL LKECEDKGEK PYYIPAGASD HPLGGLGFAR WAFEVREQEQ QMGITFDNIV VCAVTGSTMA GMVAGFKLIE KLYPDEPKKK VIGIDASATP AETRAQVLRI AKNTGEKIGL EPEDITASDI MIDERYHAGT YGVPDKQTWD AIEYGARMEA FITDPVYEGK SLAGLIDMVR KGEISGGNVL YAHLGGQLAL NAYSQIGTVA
NT Sequence
ATGACCGCTC TACCAGAGCC ATTTGCTTCT ATTCCAAGGA AAGCTCTCCT CCTTGAGCCT TCTCCAATCC ATCTACTTCC CAACATGACC GCGGATTTAG GCGGGGCTGT GAAGATATAC GCCAAACGCG ATGATCTCAA CTCCGGTCTC GCCTACGGCG GCAATAAGAC CAGGAAACTC GAGTACCTGG TCGCCGACGC GTTGGCGACA AAGTGCGACA CGCTGATTAG CATTGGCGGG ATTCAAAGCA ACCATACGCG TCAAGTAGCC GCTGCCGCAG CCCATGCGGG ACTCAAAGCA AAACTTGTCC AGGAAAAGTG GGTAGAGTGG TCCGACCCGG GTTACGAGTC CGTAGGAAAT ATCCAACTCT CCCGACTGAT GGGGGCTGAT GTCCGCATTG AGCGGATGGC TGGATTTGGA ATCGAGCATA AAGACTCGCT GAAAAATCTC CTGAAGGAAT GTGAGGACAA GGGCGAGAAA CCATATTATA TCCCCGCCGG CGCGTCAGAT CACCCGCTTG GTGGACTGGG CTTTGCACGC TGGGCGTTCG AGGTCCGGGA GCAGGAGCAG CAGATGGGCA TCACGTTTGA TAATATCGTT GTTTGTGCGG TGACGGGTTC AACGATGGCA GGAATGGTCG CTGGGTTCAA GCTTATTGAA AAACTATACC CGGATGAGCC CAAGAAGAAA GTTATTGGCA TAGATGCATC CGCCACGCCT GCGGAGACTC GCGCACAGGT CCTTCGGATC GCAAAGAATA CGGGAGAGAA AATCGGCCTT GAGCCTGAAG ATATCACCGC ATCAGATATT ATGATTGACG AACGATATCA TGCCGGAACC TATGGAGTTC CCGATAAACA GACATGGGAC GCGATCGAGT ATGGAGCTCG GATGGAAGCG TTTATCACCG ATCCCGTATA CGAAGGGAAA AGTCTGGCAG GCCTGATTGA CATGGTTCGA AAAGGGGAGA TCTCCGGAGG GAACGTCTTG TATGCGCATT TGGGAGGGCA GCTTGCTCTC AATGCATATT CGCAAATCGG GACTGTGGCA
Details for CoimA.00226.a.A1.GE27312
HARVESTED ON: 10/23/2009
SEQUENCED ON: 10/29/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTALPEPFA SIPRKALLLE PSPIHLLPNM TADLGGAVKI YAKRDDLNSG LAYGGNKTRK LEYLVADALA TKCDTLISIG GIQSNHTRQV AAAAAHAGLK AKLVQEKWVE WSDPGYESVG NIQLSRLMGA DVRIERMAGF GIEHKDSLKN LLKECEDKGE KPYYIPAGAS DHPLGGLGFA RWAFEVREQE QQMGITFDNI VVCAVTGSTM AGMVAGFKLI EKLYPDEPKK KVIGIDASAT PAETRAQVLR IAKNTGEKIG LEPEDITASD IMIDERYHAG TYGVPDKQTW DAIEYGARME AFITDPVYEG KSLAGLIDMV RKEEISGGNV LYAHLGGQLA LNAYSQIGTV A
Validated NT Sequence
ttattatgcc acagtcccga tttgcgaata tgcattgaga gcaagctgcc ctcccaaatg cgcatacaag acgttccctc cggagatctc ctcttttcga accatgtcaa tcaggcctgc cagacttttc ccttcgtata cgggatcggt gataaacgct tccatccgag ctccatactc gatcgcgtcc catgtctgtt tatcgggaac tccataggtt ccggcatgat atcgttcgtc aatcataata tctgatgcgg tgatatcttc aggctcaagg ccgattttct ctcccgtatt ctttgcgatc cgaaggacct gtgcgcgagt ctccgcaggc gtggcggatg catctatgcc aataactttc ttcttgggct catccgggta tagtttttca ataagcttga acccagcgac cattcctgcc atcgttgaac ccgtcaccgc acaaacaacg atattatcaa acgtgatgcc catctgctgc tcctgctccc ggacctcgaa cgcccagcgt gcaaagccca gtccaccaag cgggtgatct gacgcgccgg cggggatata atatggtttc tcgcccttgt cctcacattc cttcaggaga tttttcagcg agtctttatg ctcgattcca aatccagcca tccgctcaat gcggacatca gcccccatca gtcgggagag ttggatattt cctacggact cgtaacccgg gtcggaccac tctacccact tttcctggac aagttttgct ttgagtcccg catgggctgc ggcagcggct acttgacgcg tatggttgct ttgaatcccg ccaatgctaa tcagcgtgtc gcactttgtc gccaacgcgt cggcgaccag gtactcgagt ttcctggtct tattgccgcc gtaggcgaga ccggagttga gatcatcgcg tttggcgtat atcttcacag ccccgcctaa atccgcggtc atgttgggaa gtagatggat tggagaaggc tcaaggagga gagctttcct tggaatagaa gcaaatggct ctggtagagc ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTALPEPFA SIPRKALLLE PSPIHLLPNM TADLGGAVKI YAKRDDLNSG LAYGGNKTRK LEYLVADALA TKCDTLISIG GIQSNHTRQV AAAAAHAGLK AKLVQEKWVE WSDPGYESVG NIQLSRLMGA DVRIERMAGF GIEHKDSLKN LLKECEDKGE KPYYIPAGAS DHPLGGLGFA RWAFEVREQE QQMGITFDNI VVCAVTGSTM AGMVAGFKLI EKLYPDEPKK KVIGIDASAT PAETRAQVLR IAKNTGEKIG LEPEDITASD IMIDERYHAG TYGVPDKQTW DAIEYGARME AFITDPVYEG KSLAGLIDMV RKGEISGGNV LYAHLGGQLA LNAYSQIGTV A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgctcta ccagagccat ttgcttctat tccaaggaaa gctctcctcc ttgagccttc tccaatccat ctacttccca acatgaccgc ggatttaggc ggggctgtga agatatacgc caaacgcgat gatctcaact ccggtctcgc ctacggcggc aataagacca ggaaactcga gtacctggtc gccgacgcgt tggcgacaaa gtgcgacacg ctgattagca ttggcgggat tcaaagcaac catacgcgtc aagtagccgc tgccgcagcc catgcgggac tcaaagcaaa acttgtccag gaaaagtggg tagagtggtc cgacccgggt tacgagtccg taggaaatat ccaactctcc cgactgatgg gggctgatgt ccgcattgag cggatggctg gatttggaat cgagcataaa gactcgctga aaaatctcct gaaggaatgt gaggacaagg gcgagaaacc atattatatc cccgccggcg cgtcagatca cccgcttggt ggactgggct ttgcacgctg ggcgttcgag gtccgggagc aggagcagca gatgggcatc acgtttgata atatcgttgt ttgtgcggtg acgggttcaa cgatggcagg aatggtcgct gggttcaagc ttattgaaaa actatacccg gatgagccca agaagaaagt tattggcata gatgcatccg ccacgcctgc ggagactcgc gcacaggtcc ttcggatcgc aaagaatacg ggagagaaaa tcggccttga gcctgaagat atcaccgcat cagatattat gattgacgaa cgatatcatg ccggaaccta tggagttccc gataaacaga catgggacgc gatcgagtat ggagctcgga tggaagcgtt tatcaccgat cccgtatacg aagggaaaag tctggcaggc ctgattgaca tggttcgaaa aggggagatc tccggaggga acgtcttgta tgcgcatttg ggagggcagc ttgctctcaa tgcatattcg caaatcggga ctgtggcaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for CoimA.00226.a.A1.PW27303
PURIFICATION DATe: 12/7/2009
CONCENTRATION: 23.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTALPEPFA SIPRKALLLE PSPIHLLPNM TADLGGAVKI YAKRDDLNSG LAYGGNKTRK LEYLVADALA TKCDTLISIG GIQSNHTRQV AAAAAHAGLK AKLVQEKWVE WSDPGYESVG NIQLSRLMGA DVRIERMAGF GIEHKDSLKN LLKECEDKGE KPYYIPAGAS DHPLGGLGFA RWAFEVREQE QQMGITFDNI VVCAVTGSTM AGMVAGFKLI EKLYPDEPKK KVIGIDASAT PAETRAQVLR IAKNTGEKIG LEPEDITASD IMIDERYHAG TYGVPDKQTW DAIEYGARME AFITDPVYEG KSLAGLIDMV RKEEISGGNV LYAHLGGQLA LNAYSQIGTV A
Validated NT Sequence
ttattatgcc acagtcccga tttgcgaata tgcattgaga gcaagctgcc ctcccaaatg cgcatacaag acgttccctc cggagatctc ctcttttcga accatgtcaa tcaggcctgc cagacttttc ccttcgtata cgggatcggt gataaacgct tccatccgag ctccatactc gatcgcgtcc catgtctgtt tatcgggaac tccataggtt ccggcatgat atcgttcgtc aatcataata tctgatgcgg tgatatcttc aggctcaagg ccgattttct ctcccgtatt ctttgcgatc cgaaggacct gtgcgcgagt ctccgcaggc gtggcggatg catctatgcc aataactttc ttcttgggct catccgggta tagtttttca ataagcttga acccagcgac cattcctgcc atcgttgaac ccgtcaccgc acaaacaacg atattatcaa acgtgatgcc catctgctgc tcctgctccc ggacctcgaa cgcccagcgt gcaaagccca gtccaccaag cgggtgatct gacgcgccgg cggggatata atatggtttc tcgcccttgt cctcacattc cttcaggaga tttttcagcg agtctttatg ctcgattcca aatccagcca tccgctcaat gcggacatca gcccccatca gtcgggagag ttggatattt cctacggact cgtaacccgg gtcggaccac tctacccact tttcctggac aagttttgct ttgagtcccg catgggctgc ggcagcggct acttgacgcg tatggttgct ttgaatcccg ccaatgctaa tcagcgtgtc gcactttgtc gccaacgcgt cggcgaccag gtactcgagt ttcctggtct tattgccgcc gtaggcgaga ccggagttga gatcatcgcg tttggcgtat atcttcacag ccccgcctaa atccgcggtc atgttgggaa gtagatggat tggagaaggc tcaaggagga gagctttcct tggaatagaa gcaaatggct ctggtagagc ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTALPEPFA SIPRKALLLE PSPIHLLPNM TADLGGAVKI YAKRDDLNSG LAYGGNKTRK LEYLVADALA TKCDTLISIG GIQSNHTRQV AAAAAHAGLK AKLVQEKWVE WSDPGYESVG NIQLSRLMGA DVRIERMAGF GIEHKDSLKN LLKECEDKGE KPYYIPAGAS DHPLGGLGFA RWAFEVREQE QQMGITFDNI VVCAVTGSTM AGMVAGFKLI EKLYPDEPKK KVIGIDASAT PAETRAQVLR IAKNTGEKIG LEPEDITASD IMIDERYHAG TYGVPDKQTW DAIEYGARME AFITDPVYEG KSLAGLIDMV RKGEISGGNV LYAHLGGQLA LNAYSQIGTV A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCTCTA CCAGAGCCAT TTGCTTCTAT TCCAAGGAAA GCTCTCCTCC TTGAGCCTTC TCCAATCCAT CTACTTCCCA ACATGACCGC GGATTTAGGC GGGGCTGTGA AGATATACGC CAAACGCGAT GATCTCAACT CCGGTCTCGC CTACGGCGGC AATAAGACCA GGAAACTCGA GTACCTGGTC GCCGACGCGT TGGCGACAAA GTGCGACACG CTGATTAGCA TTGGCGGGAT TCAAAGCAAC CATACGCGTC AAGTAGCCGC TGCCGCAGCC CATGCGGGAC TCAAAGCAAA ACTTGTCCAG GAAAAGTGGG TAGAGTGGTC CGACCCGGGT TACGAGTCCG TAGGAAATAT CCAACTCTCC CGACTGATGG GGGCTGATGT CCGCATTGAG CGGATGGCTG GATTTGGAAT CGAGCATAAA GACTCGCTGA AAAATCTCCT GAAGGAATGT GAGGACAAGG GCGAGAAACC ATATTATATC CCCGCCGGCG CGTCAGATCA CCCGCTTGGT GGACTGGGCT TTGCACGCTG GGCGTTCGAG GTCCGGGAGC AGGAGCAGCA GATGGGCATC ACGTTTGATA ATATCGTTGT TTGTGCGGTG ACGGGTTCAA CGATGGCAGG AATGGTCGCT GGGTTCAAGC TTATTGAAAA ACTATACCCG GATGAGCCCA AGAAGAAAGT TATTGGCATA GATGCATCCG CCACGCCTGC GGAGACTCGC GCACAGGTCC TTCGGATCGC AAAGAATACG GGAGAGAAAA TCGGCCTTGA GCCTGAAGAT ATCACCGCAT CAGATATTAT GATTGACGAA CGATATCATG CCGGAACCTA TGGAGTTCCC GATAAACAGA CATGGGACGC GATCGAGTAT GGAGCTCGGA TGGAAGCGTT TATCACCGAT CCCGTATACG AAGGGAAAAG TCTGGCAGGC CTGATTGACA TGGTTCGAAA AGGGGAGATC TCCGGAGGGA ACGTCTTGTA TGCGCATTTG GGAGGGCAGC TTGCTCTCAA TGCATATTCG CAAATCGGGA CTGTGGCAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA