ChtrB.20286.a

Uncharacterized protein CT_005

CENTER ID: ChtrB.20286.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ChtrB.20286.a.B1.GE43136 full length 1 363
ChtrB.20286.a.B2.GE43590 soluble domain 1 143 363
ChtrB.20286.a.B2.GE43871 soluble domain 1 143 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.6
UniProt: O84008

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPVTPVPPQ SPQQVKGLLS RFLTAPDRHP KLRYVYDIAL IAISILCIVS IILWTQGSGL ALFAIAPALA IGALGVTLLV SDLAESQKSK EIADTVAAVS LPFILTGTAA GLMFSAIAVG GGAVILANPL FLMGSMTLGF ALMSLHRVTY QYLSNREQWK QQKKLEQVEL AAWESHLPKE SKSSALEEVR YSPRLMKRGK TWRKRAIRRK NYTPIPLVDK TLQTMQPDAL FSSTTTHSTD SEQILTSVSP QSSDTESSSS SSFHTPPNSD KELSDSNSSD SSSSSEYMDA LETVAAGDVS GITPPSKPSS SPKTTRRVVK LSRSERNAQH HRNKDQEQRQ DSSESSEEDS SSDSSQKKKP SRK
NT Sequence
atgactccag taacaccagt ccctccccaa tctccccaac aggtaaaagg gcttttatcc aggtttctga cggcacccga tcgtcacccc aaactacgct atgtttacga tattgctctt atagctatta gtattctctg tattgtgagt atcattctct ggacacaagg gtctggactc gctttatttg caatcgctcc agccttagct attggagccc taggagtcac tctgctagtc tcagatcttg ccgaatccca gaaaagtaaa gagattgctg ataccgttgc ggcagtctct cttcctttta tcctaacagg gacagctgct ggattgatgt tttctgctat tgccgtaggc ggaggcgctg taatcttagc gaatcctcta ttcctaatgg gctctatgac tctcggcttt gctctgatgt ctctgcatag agtgacctat caatatctca gcaatcgcga gcaatggaaa cagcagaaga agctcgaaca agttgagtta gctgcctggg agagccatct tcctaaagaa agcaaatcct ccgctctgga agaggttcgc tattcccctc gtttgatgaa aagagggaag acttggcgaa aacgggcaat cagaagaaaa aactatacac ctattccgtt ggtcgacaaa acattgcaaa ccatgcaacc ggatgcactc ttctcctcta caaccacaca ttctacagat agtgagcaga ttctaacttc tgtcagtcct caaagctcag ataccgaatc ctcctcttct tctagcttcc acactccacc aaatagcgat aaagaactgt ccgactcgaa ttcttctgac agcagctctt cttctgaata tatggatgct cttgaaaccg tagctgcagg agatgtctca ggaataaccc ctccatccaa accctcttct tctccgaaaa cgacacgccg cgtcgtaaag ctctctcgca gcgagagaaa tgctcagcat catcgtaata aagaccaaga gcaaagacaa gacagcagcg aatcttcgga agaggattcc tcatccgatt catctcaaaa gaagaaaccc tctcgtaaat aa
Details for ChtrB.20286.a.B1.GE43136
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT PVTPVPPQSP QQVKGLLSRF LTAPDRHPKL RYVYDIALIA ISILCIVSII LWTQGSGLAL FAIAPALAIG ALGVTLLVSD LAESQKSKEI ADTVAAVSLP FILTGTAAGL MFSAIAVGGG AVILANPLFL MGSMTLGFAL MSLHRVTYQY LSNREQWKQQ KKLEQVELAA WESHLPKESK SSALEEVRYS PRLMKRGKTW RKRAIRRKNY IPIPLVDKTL QTMQPDALFS STTTHSTDSE QILTSVSPQS SDTESSSSSS FHTPPNSDKE LSDSNSSDSS SSSEYMDALE TVAAGDVSGI TPPSKPSSSP KTTRRVVKLS RSERNAQHHR NKDQEQRQDS SESSDEESSS
Validated NT Sequence
cggaagagga ttcctcatcc gaagattcgc tgctgtcttg tctttgctct tggtctttat tacgatgatg ctgagcattt ctctcgctgc gagagagctt tacgacgcgg cgtgtcgttt tcggagaaga agagggtttg gatggagggg ttattcctga gacatctcct gcagctacgg tttcaagagc atccatatat tcagaagaag agctgctgtc agaagaattc gagtcggaca gttctttatc gctatttggt ggagtgtgga agctagaaga agaggaggat tcggtatctg agctttgagg actgacagaa gttagaatct gctcactatc tgtagaatgt gtggttgtag aggagaagag tgcatccggt tgcatggttt gcaatgtttt gtcgaccaac ggaataggta tatagttttt tcttctgatt gcccgttttc gccaagtctt ccctcttttc atcaaacgag gggaatagcg aacctcttcc agagcggagg atttgctttc tttaggaaga tggctctccc aggcagctaa ctcaacttgt tcgagcttct tctgctgttt ccattgctcg cgattactga gatattgata ggtcactcta tgcagagaca tcagagcaaa gccgagagtc atagagccca ttaggaatag aggattcgct aagattacag cgcctccgcc tacggcaata gcagaaaaca tcaatccagc agctgtccct gttaggataa aaggaagaga gactgccgca acggtatcag caatctcttt acttttctgg gattcggcaa gatctgagac tagcagagtg actcctaggg ctccaatagc taaggctgga gcgattgcaa ataaggcgag cccagaccct tgtgtccaga gaatgatact cacaatacag agaatactaa tagctataag agcaatatcg taaacatagc gtagtttggg gtgacgatcg ggtgccgtca gaaacctgga taaaagccct tttacctgtt ggggagattg gggagggact ggtgttactg gagtcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMT PVTPVPPQSP QQVKGLLSRF LTAPDRHPKL RYVYDIALIA ISILCIVSII LWTQGSGLAL FAIAPALAIG ALGVTLLVSD LAESQKSKEI ADTVAAVSLP FILTGTAAGL MFSAIAVGGG AVILANPLFL MGSMTLGFAL MSLHRVTYQY LSNREQWKQQ KKLEQVELAA WESHLPKESK SSALEEVRYS PRLMKRGKTW RKRAIRRKNY TPIPLVDKTL QTMQPDALFS STTTHSTDSE QILTSVSPQS SDTESSSSSS FHTPPNSDKE LSDSNSSDSS SSSEYMDALE TVAAGDVSGI TPPSKPSSSP KTTRRVVKLS RSERNAQHHR NKDQEQRQDS SESSEEDSSS DSSQKKKPSR K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactc cagtaacacc agtccctccc caatctcccc aacaggtaaa agggctttta tccaggtttc tgacggcacc cgatcgtcac cccaaactac gctatgttta cgatattgct cttatagcta ttagtattct ctgtattgtg agtatcattc tctggacaca agggtctgga ctcgctttat ttgcaatcgc tccagcctta gctattggag ccctaggagt cactctgcta gtctcagatc ttgccgaatc ccagaaaagt aaagagattg ctgataccgt tgcggcagtc tctcttcctt ttatcctaac agggacagct gctggattga tgttttctgc tattgccgta ggcggaggcg ctgtaatctt agcgaatcct ctattcctaa tgggctctat gactctcggc tttgctctga tgtctctgca tagagtgacc tatcaatatc tcagcaatcg cgagcaatgg aaacagcaga agaagctcga acaagttgag ttagctgcct gggagagcca tcttcctaaa gaaagcaaat cctccgctct ggaagaggtt cgctattccc ctcgtttgat gaaaagaggg aagacttggc gaaaacgggc aatcagaaga aaaaactata cacctattcc gttggtcgac aaaacattgc aaaccatgca accggatgca ctcttctcct ctacaaccac acattctaca gatagtgagc agattctaac ttctgtcagt cctcaaagct cagataccga atcctcctct tcttctagct tccacactcc accaaatagc gataaagaac tgtccgactc gaattcttct gacagcagct cttcttctga atatatggat gctcttgaaa ccgtagctgc aggagatgtc tcaggaataa cccctccatc caaaccctct tcttctccga aaacgacacg ccgcgtcgta aagctctctc gcagcgagag aaatgctcag catcatcgta ataaagacca agagcaaaga caagacagca gcgaatcttc ggaagaggat tcctcatccg attcatctca aaagaagaaa ccctctcgta aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for ChtrB.20286.a.B2.GE43590
HARVESTED ON: data unavailable
SEQUENCED ON: 2/13/2020
EXPECTED MW: 26kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LHRVTYQYLS NREQWKQQKK LEQVELAAWE SHLPKESKSS ALEEVRYSPR LMKRGKTWRK RAIRRKNYIP IPLVDKTLQT MQPDALFSST TTHSTDSEQI LTSVSPQSSD TESSSSSSFH TPPNSDKELS DSNSSDSSSS SEYMDALETV AAGDVSGITP PSKPSSSPKT TRRVVKLSRS ERNAQHHRNK DQEQRQDSSE SSDEESSSDS SQKKKPSRK
Validated NT Sequence
atggctcacc accaccacca ccatatgtct ctgcatagag tgacctatca atatctcagt aatcgcgagc aatggaaaca gcagaagaag ctcgaacaag ttgagttagc tgcctgggag agccatcttc ctaaagaaag caaatcctcc gctctggaag aggttcgcta ttcccctcgt ttgatgaaaa gagggaagac ttggcgaaaa cgggcaatca gaagaaaaaa ctatatacct attccgttgg tcgacaaaac attgcaaacc atgcaaccgg atgcactctt ctcctctaca accacacatt ctacagatag tgagcagatt ctaacttctg tcagtcctca aagctcagat accgaatcct cctcttcttc tagcttccac actccaccaa atagcgataa agaactgtcc gactcgaatt cttctgacag cagctcttct tctgaatata tggatgctct tgaaaccgta gctgcaggag atgtctcagg aataacccct ccatccaaac cctcttcttc tccgaaaacg acacgccgcg tcgtaaagct ctctcgcagc gagagaaatg ctcagcatca tcgtaataaa gaccaagagc aaagacaaga cagcagcgaa tcttcggatg aggaatcctc ttccgattca tctcaaaaga agaaaccctc tcgtaaataa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMS LHRVTYQYLS NREQWKQQKK LEQVELAAWE SHLPKESKSS ALEEVRYSPR LMKRGKTWRK RAIRRKNYTP IPLVDKTLQT MQPDALFSST TTHSTDSEQI LTSVSPQSSD TESSSSSSFH TPPNSDKELS DSNSSDSSSS SEYMDALETV AAGDVSGITP PSKPSSSPKT TRRVVKLSRS ERNAQHHRNK DQEQRQDSSE SSEEDSSSDS SQKKKPSRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc tgcatagagt gacctatcaa tatctcagca atcgcgagca atggaaacag cagaagaagc tcgaacaagt tgagttagct gcctgggaga gccatcttcc taaagaaagc aaatcctccg ctctggaaga ggttcgctat tcccctcgtt tgatgaaaag agggaagact tggcgaaaac gggcaatcag aagaaaaaac tatacaccta ttccgttggt cgacaaaaca ttgcaaacca tgcaaccgga tgcactcttc tcctctacaa ccacacattc tacagatagt gagcagattc taacttctgt cagtcctcaa agctcagata ccgaatcctc ctcttcttct agcttccaca ctccaccaaa tagcgataaa gaactgtccg actcgaattc ttctgacagc agctcttctt ctgaatatat ggatgctctt gaaaccgtag ctgcaggaga tgtctcagga ataacccctc catccaaacc ctcttcttct ccgaaaacga cacgccgcgt cgtaaagctc tctcgcagcg agagaaatgc tcagcatcat cgtaataaag accaagagca aagacaagac agcagcgaat cttcggaaga ggattcctca tccgattcat ctcaaaagaa gaaaccctct cgtaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for ChtrB.20286.a.B2.GE43871
HARVESTED ON: data unavailable
SEQUENCED ON: 12/2/2020
EXPECTED MW: 26kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LHRVTYQYLS NREQWKQQKK LEQVELAAWE SHLPKESKSS ALEEVRYSPR LMKRGKTWRK RAIRRKNYIP IPLVDKTLQT MQPDALFSST TTHSTDSEQI LTSVSPQSSD TESSSSSSFH TPPNSDKELS DSNSSDSSSS SEYMDALETV AAGDVSGITP PSKPSSSPKT TRRVVKLSRS ERNAQHHRNK DQEQRQDSSE SSDEESSSDS SQKKKPSRK
Validated NT Sequence
atggctcacc accaccacca ccatatgtct ctgcatagag tgacctatca atatctcagt aatcgcgagc aatggaaaca gcagaagaag ctcgaacaag ttgagttagc tgcctgggag agccatcttc ctaaagaaag caaatcctcc gctctggaag aggttcgcta ttcccctcgt ttgatgaaaa gagggaagac ttggcgaaaa cgggcaatca gaagaaaaaa ctatatacct attccgttgg tcgacaaaac attgcaaacc atgcaaccgg atgcactctt ctcctctaca accacacatt ctacagatag tgagcagatt ctaacttctg tcagtcctca aagctcagat accgaatcct cctcttcttc tagcttccac actccaccaa atagcgataa agaactgtcc gactcgaatt cttctgacag cagctcttct tctgaatata tggatgctct tgaaaccgta gctgcaggag atgtctcagg aataacccct ccatccaaac cctcttcttc tccgaaaacg acacgccgcg tcgtaaagct ctctcgcagc gagagaaatg ctcagcatca tcgtaataaa gaccaagagc aaagacaaga cagcagcgaa tcttcggatg aggaatcctc ttccgattca tctcaaaaga agaaaccctc tcgtaaataa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMS LHRVTYQYLS NREQWKQQKK LEQVELAAWE SHLPKESKSS ALEEVRYSPR LMKRGKTWRK RAIRRKNYTP IPLVDKTLQT MQPDALFSST TTHSTDSEQI LTSVSPQSSD TESSSSSSFH TPPNSDKELS DSNSSDSSSS SEYMDALETV AAGDVSGITP PSKPSSSPKT TRRVVKLSRS ERNAQHHRNK DQEQRQDSSE SSEEDSSSDS SQKKKPSRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc tgcatagagt gacctatcaa tatctcagca atcgcgagca atggaaacag cagaagaagc tcgaacaagt tgagttagct gcctgggaga gccatcttcc taaagaaagc aaatcctccg ctctggaaga ggttcgctat tcccctcgtt tgatgaaaag agggaagact tggcgaaaac gggcaatcag aagaaaaaac tatacaccta ttccgttggt cgacaaaaca ttgcaaacca tgcaaccgga tgcactcttc tcctctacaa ccacacattc tacagatagt gagcagattc taacttctgt cagtcctcaa agctcagata ccgaatcctc ctcttcttct agcttccaca ctccaccaaa tagcgataaa gaactgtccg actcgaattc ttctgacagc agctcttctt ctgaatatat ggatgctctt gaaaccgtag ctgcaggaga tgtctcagga ataacccctc catccaaacc ctcttcttct ccgaaaacga cacgccgcgt cgtaaagctc tctcgcagcg agagaaatgc tcagcatcat cgtaataaag accaagagca aagacaagac agcagcgaat cttcggaaga ggattcctca tccgattcat ctcaaaagaa gaaaccctct cgtaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t