ChtrB.20284.a

Uncharacterized protein

CENTER ID: ChtrB.20284.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20284.a.B2.GE43588 soluble domain 121 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.939
UniProt: O84858

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGFGTVRGKG KAVKSFFLRP LQNLEVGLFS LPIVLLLGEI GCVSSISSVS LVAVLSIVGV FVALVSFFRS WGYGLSVVGA IFFGLALCNN FPVSVFWGGL LTVSFIISYG ILLLSVSLVE GHIKEKAVSL SELTASHNSL QDSYNREVQE RKEKELLAQS KITALEQELS VSHEQLQEVS RKYTHASEDL QILIDQRDSW LKDYMTLHQE YVRVVAGDEE NVIFPWKVFQ GNSEKDSGYQ QRVQDAEHKI AHLEKLCEEE NSGKRYAEEC LDKALADLLE STRLREILEK EIFQKDEEIA SLKQEIAAEK LLSSSVSDDR AAYKGKYLQL REQFTVKDSF LKKARRERFL AQEQLLVLKR AKEEEASNLS TTDSFSIIQN LLLQIEALEE EVTYLEELVL HNQNR
NT Sequence
atgggattcg gaactgtgag agggaaagga aaagctgtga agtccttctt cctaaggcct ctacagaatc ttgaagtagg gctcttttca ttacctatag tgttattgtt gggggagatt gggtgtgtat cttcaatctc ttccgtttct ttagtagctg ttttaagtat tgtaggcgtt ttcgttgctc tagtttcctt cttccgtagt tggggctatg ggctctctgt tgtaggggct atatttttcg ggttggctct ttgtaacaat ttccctgtgt ctgttttttg gggaggattg ttaactgtct cttttattat ttcttatggg attcttctgc tgagtgtgtc tttggttgaa ggacatatta aggagaaggc tgtatcttta tcagagctga cggcatcgca caattccctt caagactcct ataaccgcga agtgcaagaa cgaaaagaaa aggagcttct tgcccagagt aagataactg ctttagaaca agagctttct gtttcgcatg agcaactcca agaggtttcc aggaaatata cgcatgcaag tgaagattta cagattctta ttgatcagag ggatagctgg ttaaaagatt acatgactct ccatcaagag tatgtgcgtg tagttgctgg agatgaagag aatgtcattt tcccttggaa agtctttcag ggtaattctg aaaaggattc tggttatcag cagcgggttc aggatgctga acataagatc gctcatttag agaagctctg tgaagaggaa aatagcggga agcgctatgc tgaggagtgc ttggataagg ccttagcaga tttattagag tctactcggc ttagggaaat tctagagaaa gagatcttcc aaaaggatga ggagatcgca tctttgaaac aagagattgc agcggaaaag cttttgagtt cttcggtcag tgatgacaga gcggcttata agggtaagta cctgcaatta agggagcagt tcacggttaa ggattccttt ctgaaaaaag ctagacgcga acgcttctta gctcaagagc aacttttagt tttgaagcga gctaaagaag aagaagcctc gaatttgtct acaacggata gtttttctat tattcaaaat cttttgctac aaattgaggc actagaagaa gaagttactt atctagaaga gttagtactt cacaaccaga atcggtaa
Details for ChtrB.20284.a.B2.GE43588
HARVESTED ON: 2/3/2020
SEQUENCED ON: 2/13/2020
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 88
PERCENT COVERAGE: 46
Validated AA Sequence
MAHHHHHHMG HIKEKAVSLS ELTASHNSLQ DSYNREVQER KEKXXLAQSK ITALEQELSV SHEQLQEVSR KYTHTSEDLQ ILIDQRDSWX KDYXTLXQEY VRVVAGDEEN VIFPWKXFXG NSEKGFWLSA AGXGX
Validated NT Sequence
tcctataggg agaccacanc ggtttccctc tagaaataat tttgtttaac tttnnnnngg ngnnntacca tggctcacca ccaccaccac catatgggac atattaagga gaaggctgta tctttatcag agctgacggc atcgcacaat tcccttcaag actcctataa ccgcgaagtg caagaacgaa aagaaaaggn gnttcttgcc cagagtaaga taactgcttt agaacaagag ctttctgttt cgcatgagca actccaagag gtttccagga aatatacgca tacaagtgaa gatttacaga ttcttattga tcagagggat agctggntaa aagattacnt gactctccnt caagagtatg tgcgtgtagt tgctggagat gaagagaatg tcattttccc ttggaaagnc tttcanggta attctgaaaa aggattctgg ttatcagcag cgggntnagg atgntgaaca taanatcgct catttataga agctctgtga agaggaaaat nncgggaagc gctatgctga tgagtgcttg gataaggcct tannnnattt atttacagtc tactcggctt anggtaantt ctananaaag agattctttc caaaaggatg aggagatcgc atcttttgaa acnanagatn gcancggaaa agcttttgag nttcntcngn cagngatgac agagcngctt ataagggtaa agtaccctgc tattaanggg agcnnnttan cggtnaanga ttccttttct gaaaaaannt anacgcnaac gcttcttatc tcaanagcaa ctttttnntt ttgaagcgag ctaangaaaa aananannct tnnnntgtct accnacngga tantttttct attattcaan anccttttgn tnactaantg naggcatntn naaanaagaa attactttat tntagaagnn ttantacttn nccaacncgn anttgntnnn tgnngnnnnn ataggantcc nggnntgcnn ancnaaannc cnaaangnaa nncttnnant tgnntngtnn ncnnnccnnc tgnannantn aacttannnn anancccntn gggngnnctc nnnannnggn nnnnnnnnng gntttttttn nncttnaaan ggnagnaann tannntnncc tggnannnnn ncccnng
Expected Protein Sequence
MAHHHHHHGH IKEKAVSLSE LTASHNSLQD SYNREVQERK EKELLAQSKI TALEQELSVS HEQLQEVSRK YTHASEDLQI LIDQRDSWLK DYMTLHQEYV RVVAGDEENV IFPWKVFQGN SEKDSGYQQR VQDAEHKIAH LEKLCEEENS GKRYAEECLD KALADLLEST RLREILEKEI FQKDEEIASL KQEIAAEKLL SSSVSDDRAA YKGKYLQLRE QFTVKDSFLK KARRERFLAQ EQLLVLKRAK EEEASNLSTT DSFSIIQNLL LQIEALEEEV TYLEELVLHN QNR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggacata ttaaggagaa ggctgtatct ttatcagagc tgacggcatc gcacaattcc cttcaagact cctataaccg cgaagtgcaa gaacgaaaag aaaaggagct tcttgcccag agtaagataa ctgctttaga acaagagctt tctgtttcgc atgagcaact ccaagaggtt tccaggaaat atacgcatgc aagtgaagat ttacagattc ttattgatca gagggatagc tggttaaaag attacatgac tctccatcaa gagtatgtgc gtgtagttgc tggagatgaa gagaatgtca ttttcccttg gaaagtcttt cagggtaatt ctgaaaagga ttctggttat cagcagcggg ttcaggatgc tgaacataag atcgctcatt tagagaagct ctgtgaagag gaaaatagcg ggaagcgcta tgctgaggag tgcttggata aggccttagc agatttatta gagtctactc ggcttaggga aattctagag aaagagatct tccaaaagga tgaggagatc gcatctttga aacaagagat tgcagcggaa aagcttttga gttcttcggt cagtgatgac agagcggctt ataagggtaa gtacctgcaa ttaagggagc agttcacggt taaggattcc tttctgaaaa aagctagacg cgaacgcttc ttagctcaag agcaactttt agttttgaag cgagctaaag aagaagaagc ctcgaatttg tctacaacgg atagtttttc tattattcaa aatcttttgc tacaaattga ggcactagaa gaagaagtta cttatctaga agagttagta cttcacaacc agaatcggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat