SSGCID: Target Page

ChtrB.20275.a

Uncharacterized protein CT_036

CENTER ID:	ChtrB.20275.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.20275.a.B1.GE42967	full length		1	403
ChtrB.20275.a.B2.GE43585	soluble domain 1		87	403

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.20275.a.B2.PW38868	soluble domain 1		87	403

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272561.5.peg.41
UniProt:	O84039

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFPIECHTLQ TSFKQVLSLV AEKITTKAFV IFSVLFSLII GFIASCGFLF AGPPAFIASG LCFALLVSVV SFFGCQKLIP YGIQHLMSYV KSIPSLSSSL IDFLKTESKS ISSLYPNPGL KECFKGASPK YKKFFFDHPE KLLSAAFTDW TPQIIPSDSG QPRTIILSHS SLPFSLTLST LDFETLHTHL IKSNALTCRV GYAHQLPSGN PVMREAKEGV LQQHYDTGNE TFFISIQESK QLQQEELFKK LFSHYAQITE HNLSNEILLL EPLKTPLHTP KARTLELLAL FCALEQLRYT KVADWRTKKL APIFPLDYED FFTLFMKKQH YTLPGNVSNM RILSPVRPVS ETALTTIIIS GLEEEDKLGL LGQVQPFLFD AEEAHPQRGE SILIQNVLDD ITQ

NT Sequence

atgttcccaa tagaatgtca caccctacag acttctttta aacaagttct ctctttggtt gctgaaaaga taacaaccaa agcttttgtg attttttctg tgttattttc cttaattata ggattcatcg catcgtgtgg attccttttt gctggcccgc cggctttcat agcctccgga ctttgctttg ctctgcttgt ttccgtggtt agtttttttg ggtgtcagaa gctcatccct tacggaattc aacatctgat gtcgtatgta aaatccatcc cctctctctc atcttctctc attgattttc taaaaacgga atccaaaagc atttcaagcc tataccccaa tcctggctta aaagaatgtt tcaagggtgc ctctcctaaa tataaaaagt ttttctttga tcacccagaa aagcttcttt ctgcagcttt tacagactgg acaccacaaa tcatcccttc ggattcaggg caacctcgaa ccattatcct atcgcactcc agtttgcctt tctccctcac actatccact ctggattttg aaacgctaca tacccatctc atcaagtcca atgctctcac ctgtcgagtg ggttatgcac accaattacc ttccggcaat cctgttatgc gcgaagccaa agaaggagtt ctacaacaac attatgatac aggaaatgaa accttcttca tttctattca ggaaagcaag cagctgcaac aagaagagct ctttaaaaaa ctctttagtc attatgcaca gatcacagaa cacaacctat ctaatgagat tttattgcta gaacccctca aaactcctct gcacacacca aaagcacgca ctctagagtt gctagcacta ttctgcgctt tagaacagtt gcgttataca aaagttgcgg attggagaac taagaaactc gctcccatat tcccgttgga ttatgaagat ttctttactc tattcatgaa aaagcaacac tacacgcttc ccggtaatgt atcgaatatg cgtatcctat ctcctgtacg acctgttagt gagacagcac taaccaccat tattatttct ggattagaag aggaagataa attgggtcta ctaggacaag tgcaaccgtt cttatttgat gctgaggaag cacatcctca aagaggtgag agcattttaa tacaaaacgt gctcgacgac attactcaat ag

Details for ChtrB.20275.a.B1.GE42967

HARVESTED ON:	3/4/2019
SEQUENCED ON:	3/12/2019
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMF PIECHTLQTS FKQVLSLVAE KITTKAFVIF SVLFSLIIGF IASCGFLFAG PPAFIASGLC FALLVSVVSF FGCQKLIPYG IQHLMSYVKS IPSLSSSLID FLKTESKSIS SLYPNPGLKE CFKGASPKYK KFFFDHPEKL LSAAFTDWTP QIIPSDSGQP RTIILSHSSL PFSLTLSTLD FETLHTHLIK SNALTCRVGY AHQLPSGNPV MREAKEGVLQ QHYDTGNETF FISIQESKQL QQEELFKKLF SHYAQITEHN LSNEILLLEP LKTPLHTPKA RTLELLALFC ALEQLRYTKV ADWRTKKLAP IFPLDYEDFF TLFMKKQHYT LPGNVSNMRI LSPVRPVSET ALTTIIISGL EEEDKLGLLG QVQPFLFDAE EAHPQRGESI LIQNVLDDIT Q

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcc caatagaatg tcacacccta cagacttctt ttaaacaagt tctctctttg gttgctgaaa agataacaac caaagctttt gtgatttttt ctgtgttatt ttccttaatt ataggattca tcgcatcgtg tggattcctt tttgctggcc cgccggcttt catagcctcc ggactttgct ttgctctgct tgtttccgtg gttagttttt ttgggtgtca gaagctcatc ccttacggaa ttcaacatct gatgtcgtat gtaaaatcca tcccctctct ctcatcttct ctcattgatt ttctaaaaac ggaatccaaa agcatttcaa gcctataccc caatcctggc ttaaaagaat gtttcaaggg tgcctctcct aaatataaaa agtttttctt tgatcaccca gaaaagcttc tttctgcagc ttttacagac tggacaccac aaatcatccc ttcggattca gggcaacctc gaaccattat cctatcgcac tccagtttgc ctttctccct cacactatcc actctggatt ttgaaacgct acatacccat ctcatcaagt ccaatgctct cacctgtcga gtgggttatg cacaccaatt accttccggc aatcctgtta tgcgcgaagc caaagaagga gttctacaac aacattatga tacaggaaat gaaaccttct tcatttctat tcaggaaagc aagcagctgc aacaagaaga gctctttaaa aaactcttta gtcattatgc acagatcaca gaacacaacc tatctaatga gattttattg ctagaacccc tcaaaactcc tctgcacaca ccaaaagcac gcactctaga gttgctagca ctattctgcg ctttagaaca gttgcgttat acaaaagttg cggattggag aactaagaaa ctcgctccca tattcccgtt ggattatgaa gatttcttta ctctattcat gaaaaagcaa cactacacgc ttcccggtaa tgtatcgaat atgcgtatcc tatctcctgt acgacctgtt agtgagacag cactaaccac cattattatt tctggattag aagaggaaga taaattgggt ctactaggac aagtgcaacc gttcttattt gatgctgagg aagcacatcc tcaaagaggt gagagcattt taatacaaaa cgtgctcgac gacattactc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for ChtrB.20275.a.B2.GE43585

HARVESTED ON:	2/3/2020
SEQUENCED ON:	2/13/2020
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	20
PERCENT COVERAGE:	27

Validated AA Sequence

MLSPVEWVMH TNYLPXXLLC AKPXKEFYNX IMIQEMKPSS FLFXKAXXCN XKXSLKNSLX XMHRSXNTTY LMRFYCXNXS XTPLHTXX

Validated NT Sequence

tggctcnccn ccnccaccac catatgtcgn atgtaannnn nntcccctct ctctcntctt ctctcattga ttttctaaaa acnggaatcc aaaagcattt caagcctata ccccaatcct ggcttaaaag aatgtttcna gggcgcctct cctaaatata aaaagttttt ctttgatcac ccagaaaagc ttctttctgc nncttttaca gactggacac cacaaatcat cccttcggat tcagggcaac ctcgaaccat tatcctgtcg cactccagtt tgcctttctc cctcacacta tccactctgg attttgaaac gctacatacc tatctcatca agtccaatgc tctcacctgt cgagtgggtt atgcacacca attaccttcc ggnnnncctg ttatgcgcga agccnaanaa ggagttctac aacancatta tgatacagga aatgaaacct tcttcatttc tattcangaa agcaagnnnc tgcaacaana aganctcttt aaaaaactct ttannnncta tgcacagatc atanaacaca acctatctaa tgagatttta ttgctanaac ncctcnnaaa ctcctctgca cacancanaa gca

Expected Protein Sequence

MAHHHHHHMS YVKSIPSLSS SLIDFLKTES KSISSLYPNP GLKECFKGAS PKYKKFFFDH PEKLLSAAFT DWTPQIIPSD SGQPRTIILS HSSLPFSLTL STLDFETLHT HLIKSNALTC RVGYAHQLPS GNPVMREAKE GVLQQHYDTG NETFFISIQE SKQLQQEELF KKLFSHYAQI TEHNLSNEIL LLEPLKTPLH TPKARTLELL ALFCALEQLR YTKVADWRTK KLAPIFPLDY EDFFTLFMKK QHYTLPGNVS NMRILSPVRP VSETALTTII ISGLEEEDKL GLLGQVQPFL FDAEEAHPQR GESILIQNVL DDITQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt atgtaaaatc catcccctct ctctcatctt ctctcattga ttttctaaaa acggaatcca aaagcatttc aagcctatac cccaatcctg gcttaaaaga atgtttcaag ggtgcctctc ctaaatataa aaagtttttc tttgatcacc cagaaaagct tctttctgca gcttttacag actggacacc acaaatcatc ccttcggatt cagggcaacc tcgaaccatt atcctatcgc actccagttt gcctttctcc ctcacactat ccactctgga ttttgaaacg ctacataccc atctcatcaa gtccaatgct ctcacctgtc gagtgggtta tgcacaccaa ttaccttccg gcaatcctgt tatgcgcgaa gccaaagaag gagttctaca acaacattat gatacaggaa atgaaacctt cttcatttct attcaggaaa gcaagcagct gcaacaagaa gagctcttta aaaaactctt tagtcattat gcacagatca cagaacacaa cctatctaat gagattttat tgctagaacc cctcaaaact cctctgcaca caccaaaagc acgcactcta gagttgctag cactattctg cgctttagaa cagttgcgtt atacaaaagt tgcggattgg agaactaaga aactcgctcc catattcccg ttggattatg aagatttctt tactctattc atgaaaaagc aacactacac gcttcccggt aatgtatcga atatgcgtat cctatctcct gtacgacctg ttagtgagac agcactaacc accattatta tttctggatt agaagaggaa gataaattgg gtctactagg acaagtgcaa ccgttcttat ttgatgctga ggaagcacat cctcaaagag gtgagagcat tttaatacaa aacgtgctcg acgacattac tcaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ChtrB.20275.a.B2.PW38868

PURIFICATION DATe:	7/9/2020
CONCENTRATION:	52mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	20
PERCENT COVERAGE:	27

Protocol Notes

notes unavailable

Validated AA Sequence

MLSPVEWVMH TNYLPXXLLC AKPXKEFYNX IMIQEMKPSS FLFXKAXXCN XKXSLKNSLX XMHRSXNTTY LMRFYCXNXS XTPLHTXX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGT ATGTAAAATC CATCCCCTCT CTCTCATCTT CTCTCATTGA TTTTCTAAAA ACGGAATCCA AAAGCATTTC AAGCCTATAC CCCAATCCTG GCTTAAAAGA ATGTTTCAAG GGTGCCTCTC CTAAATATAA AAAGTTTTTC TTTGATCACC CAGAAAAGCT TCTTTCTGCA GCTTTTACAG ACTGGACACC ACAAATCATC CCTTCGGATT CAGGGCAACC TCGAACCATT ATCCTATCGC ACTCCAGTTT GCCTTTCTCC CTCACACTAT CCACTCTGGA TTTTGAAACG CTACATACCC ATCTCATCAA GTCCAATGCT CTCACCTGTC GAGTGGGTTA TGCACACCAA TTACCTTCCG GCAATCCTGT TATGCGCGAA GCCAAAGAAG GAGTTCTACA ACAACATTAT GATACAGGAA ATGAAACCTT CTTCATTTCT ATTCAGGAAA GCAAGCAGCT GCAACAAGAA GAGCTCTTTA AAAAACTCTT TAGTCATTAT GCACAGATCA CAGAACACAA CCTATCTAAT GAGATTTTAT TGCTAGAACC CCTCAAAACT CCTCTGCACA CACCAAAAGC ACGCACTCTA GAGTTGCTAG CACTATTCTG CGCTTTAGAA CAGTTGCGTT ATACAAAAGT TGCGGATTGG AGAACTAAGA AACTCGCTCC CATATTCCCG TTGGATTATG AAGATTTCTT TACTCTATTC ATGAAAAAGC AACACTACAC GCTTCCCGGT AATGTATCGA ATATGCGTAT CCTATCTCCT GTACGACCTG TTAGTGAGAC AGCACTAACC ACCATTATTA TTTCTGGATT AGAAGAGGAA GATAAATTGG GTCTACTAGG ACAAGTGCAA CCGTTCTTAT TTGATGCTGA GGAAGCACAT CCTCAAAGAG GTGAGAGCAT TTTAATACAA AACGTGCTCG ACGACATTAC TCAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT