SSGCID: Target Page

ChtrB.20273.a

Uncharacterized protein

CENTER ID:	ChtrB.20273.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.20273.a.B1.GE42965	full length		1	810

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272561.5.peg.167
UniProt:	O84155

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKPSFLYVI QPFSVFNPRL GRFSTDSDTY IEEENRLASF IESLPLEIFD IPSFMETAIS NSPYILSWET TKDGALFTIL EPKLSACAAT CLVAPSIQMK SDAELLEEIK QALLRSSHDG VKYRITRESF SPEKKTPKVA LVDDDIELIR NVDFLGRAVD IVKLDPINIL NTVSEENILD YSFTRETAQL SADGRFGIPP GTKLFPKPSF DVEISTSIFE ETTSFTRSFS ASVTFSVPDL AATMPLQSPP MVENGQKEIC VIQKHLFPSY SPKLVDIVKR YKREAKILIN KLAFGMLWRH RAKSQILTEG SVRLDLQGFT ESKYNYQIQV GSHTIAAVLI DMDISKIQSK SEQAYAIRKI KSGFQRSLDD YHIYQIERKQ TFSFSPKHRS LSSTSHSEDS DLDLSEAAAF SGSLTCEFVK KSTQHAKNTV TCSTAAHSLY TLKEDDSSNP SEKRLDSCFR NWIENKLSAN SPDSWSAFIQ KFGTHYIASA TFGGIGFQVL KLSFEQVEDL HSKKISLETA AANSLLKGSV SSSTESGYSS YSSTSSSHTV FLGGTVLPSV HDERLDFKDW SESVHLEPVP IQVSLQPITN LLVPLHFPNI GAAELSNKRE SLQQAIRVYL KEHKVDEQGE RTTFTSGIDN PSSWFTLEAA HSPLIVSTPY IASWSTLPYL FPTLRERSSA TPIVFYFCVD NNEHASQKIL NQSYCFLGSL PIRQKIFGSE FASFPYLSFY GNAKEAYFDN TYYPTRCGWI VEKLNTTQDQ FLRDGDEVRL KHVSSGKYLA TTPLKDTHGT LTRTTNCEDA IFIIKKSSGY

NT Sequence

atgactaagc cttctttctt atacgttatt caaccttttt ccgtatttaa tccacgatta ggacgtttct ctacagactc agatacttat atcgaagaag aaaaccgcct agcatcgttc attgagagtt tgccactgga gatcttcgat ataccttctt tcatggaaac cgcgatttcc aatagcccct atattttatc ttgggagaca actaaagacg gcgctctgtt cactattctt gaacccaaac tctcagcttg cgcagccact tgcctggtag ccccttctat acaaatgaaa tccgatgcgg agctcctaga agaaattaag caagcgttat tacgcagctc tcatgacggt gtgaaatatc gcatcaccag agaatccttc tctccagaaa agaaaactcc taaggttgct ctagtcgatg acgatattga attgattcgc aatgtcgact ttttgggtag agctgttgac attgtcaaat tagaccctat taatattctg aataccgtaa gcgaagagaa tattctagat tactctttta caagagaaac ggctcagctg agcgcggatg gtcgttttgg tattcctcca gggactaagc tattccctaa accttctttt gatgtagaaa tcagtacctc cattttcgaa gaaacaactt catttactcg aagtttttct gcatcggtta cttttagtgt accagacctc gcggcgacta tgcctcttca aagccctccc atggtagaaa atggtcaaaa agaaatttgt gtcattcaaa aacacttatt cccaagctac tctcctaaac tagtcgatat tgttaaacga tacaaaagag aggctaagat cttgattaac aagcttgcct ttggaatgtt atggcgacat cgggctaaaa gccaaatcct caccgaggga agcgtacgtc tagacttaca aggattcaca gaatcgaagt acaattacca gattcaagta ggatcccata cgattgcagc tgtattaatc gatatggata tttccaagat tcaatccaaa tcagaacaag cttatgcaat taggaaaatc aaatcaggct ttcaacgtag cttggatgac tatcatattt atcaaattga aagaaaacaa accttttctt tttctccgaa gcatcgcagc ctctcatcca catcccattc cgaagattct gatttggatc tttctgaagc agccgccttt tcaggaagtc ttacctgcga gtttgtaaaa aaaagcactc aacatgccaa gaataccgtc acatgttcca cagccgctca ttccctatac acactcaaag aagatgacag ctcgaacccc tctgaaaaac gattagatag ttgtttccgc aattggattg aaaacaaact aagcgccaat tctccagatt cctggtcagc gtttattcaa aaattcggaa cacactatat tgcatcagca acttttggag ggataggttt ccaagtgctc aaactatctt ttgaacaggt ggaggatcta catagcaaaa agatctcctt agaaaccgca gcagccaact ctctattaaa aggttctgta tccagcagca cagaatctgg atactccagc tatagctcca cgtcttcttc tcatacggta tttttaggag gaacggtctt accttcggtt catgatgaac gtttagactt taaagattgg tcggaaagtg tgcacctgga acctgttcct atccaggttt ctttacaacc tataacgaat ttactagttc ctctccattt tcctaatatc ggtgctgcag agctctctaa taaacgagaa tctcttcaac aagcgattcg agtctatctc aaagaacata aagtagatga gcaaggagaa cgtactacat ttacatcagg aatcgataat ccttcttcct ggtttacctt agaagctgcc cactctcctc ttatagtcag tactccttac attgcttcgt ggtctacgct tccttatttg ttcccaacat taagagaacg ttcttcggca acccctatcg ttttctattt ttgtgtagat aataatgaac atgcttcgca aaaaatatta aaccaatcgt attgcttcct cgggtccttg cctattcgac aaaaaatttt tggtagcgaa tttgctagtt tcccctatct atctttctat ggaaatgcaa aagaggcgta ctttgataac acgtactacc caacgcgttg tgggtggatt gttgaaaagt taaatactac acaagatcaa ttcctccggg atggagacga ggtgcgacta aaacatgttt ccagcggaaa gtatctagca acaactcctc ttaaggatac ccatggtaca ctcacgcgta caacgaactg tgaagatgct atctttatta ttaaaaaatc ttcaggttat tga

Details for ChtrB.20273.a.B1.GE42965

HARVESTED ON:	3/4/2019
SEQUENCED ON:	3/12/2019
EXPECTED MW:	92kDa
OBSERVED MW:	92kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT KPSFLYVIQP FSVFNPRLGR FSTDSDTYIE EENRLASFIE SLPLEIFDIP SFMETAISNS PYILSWETTK DGALFTILEP KLSACAATCL VAPSIQMKSD AELLEEIKQA LLRSSHDGVK YRITRESFSP EKKTPKVALV DDDIELIRNV DFLGRAVDIV KLDPINILNT VSEENILDYS FTRETAQLSA DGRFGIPPGT KLFPKPSFDV EISTSIFEET TSFTRSFSAS VTFSVPDLAA TMPLQSPPMV ENGQKEICVI QKHLFPSYSP KLVDIVKRYK REAKILINKL AFGMLWRHRA KSQILTEGSV RLDLQGFTES KYNYQIQVGS HTIAAVLIDM DISKIQSKSE QAYAIRKIKS GFQRSLDDYH IYQIERKQTF SFSPKHRSLS STSHSEDSDL DLSEAAAFSG SLTCEFVKKS TQHAKNTVTC STAAHSLYTL KEDDSSNPSE KRLDSCFRNW IENKLSANSP DSWSAFIQKF GTHYIASATF GGIGFQVLKL SFEQVEDLHS KKISLETAAA NSLLKGSVSS STESGYSSYS STSSSHTVFL GGTVLPSVHD ERLDFKDWSE SVHLEPVPIQ VSLQPITNLL VPLHFPNIGA AELSNKRESL QQAIRVYLKE HKVDEQGERT TFTSGIDNPS SWFTLEAAHS PLIVSTPYIA SWSTLPYLFP TLRERSSATP IVFYFCVDNN EHASQKILNQ SYCFLGSLPI RQKIFGSEFA SFPYLSFYGN AKEAYFDNTY YPTRCGWIVE KLNTTQDQFL RDGDEVRLKH VSSGKYLATT PLKDTHGTLT RTTNCEDAIF IIKKSSGY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta agccttcttt cttatacgtt attcaacctt tttccgtatt taatccacga ttaggacgtt tctctacaga ctcagatact tatatcgaag aagaaaaccg cctagcatcg ttcattgaga gtttgccact ggagatcttc gatatacctt ctttcatgga aaccgcgatt tccaatagcc cctatatttt atcttgggag acaactaaag acggcgctct gttcactatt cttgaaccca aactctcagc ttgcgcagcc acttgcctgg tagccccttc tatacaaatg aaatccgatg cggagctcct agaagaaatt aagcaagcgt tattacgcag ctctcatgac ggtgtgaaat atcgcatcac cagagaatcc ttctctccag aaaagaaaac tcctaaggtt gctctagtcg atgacgatat tgaattgatt cgcaatgtcg actttttggg tagagctgtt gacattgtca aattagaccc tattaatatt ctgaataccg taagcgaaga gaatattcta gattactctt ttacaagaga aacggctcag ctgagcgcgg atggtcgttt tggtattcct ccagggacta agctattccc taaaccttct tttgatgtag aaatcagtac ctccattttc gaagaaacaa cttcatttac tcgaagtttt tctgcatcgg ttacttttag tgtaccagac ctcgcggcga ctatgcctct tcaaagccct cccatggtag aaaatggtca aaaagaaatt tgtgtcattc aaaaacactt attcccaagc tactctccta aactagtcga tattgttaaa cgatacaaaa gagaggctaa gatcttgatt aacaagcttg cctttggaat gttatggcga catcgggcta aaagccaaat cctcaccgag ggaagcgtac gtctagactt acaaggattc acagaatcga agtacaatta ccagattcaa gtaggatccc atacgattgc agctgtatta atcgatatgg atatttccaa gattcaatcc aaatcagaac aagcttatgc aattaggaaa atcaaatcag gctttcaacg tagcttggat gactatcata tttatcaaat tgaaagaaaa caaacctttt ctttttctcc gaagcatcgc agcctctcat ccacatccca ttccgaagat tctgatttgg atctttctga agcagccgcc ttttcaggaa gtcttacctg cgagtttgta aaaaaaagca ctcaacatgc caagaatacc gtcacatgtt ccacagccgc tcattcccta tacacactca aagaagatga cagctcgaac ccctctgaaa aacgattaga tagttgtttc cgcaattgga ttgaaaacaa actaagcgcc aattctccag attcctggtc agcgtttatt caaaaattcg gaacacacta tattgcatca gcaacttttg gagggatagg tttccaagtg ctcaaactat cttttgaaca ggtggaggat ctacatagca aaaagatctc cttagaaacc gcagcagcca actctctatt aaaaggttct gtatccagca gcacagaatc tggatactcc agctatagct ccacgtcttc ttctcatacg gtatttttag gaggaacggt cttaccttcg gttcatgatg aacgtttaga ctttaaagat tggtcggaaa gtgtgcacct ggaacctgtt cctatccagg tttctttaca acctataacg aatttactag ttcctctcca ttttcctaat atcggtgctg cagagctctc taataaacga gaatctcttc aacaagcgat tcgagtctat ctcaaagaac ataaagtaga tgagcaagga gaacgtacta catttacatc aggaatcgat aatccttctt cctggtttac cttagaagct gcccactctc ctcttatagt cagtactcct tacattgctt cgtggtctac gcttccttat ttgttcccaa cattaagaga acgttcttcg gcaaccccta tcgttttcta tttttgtgta gataataatg aacatgcttc gcaaaaaata ttaaaccaat cgtattgctt cctcgggtcc ttgcctattc gacaaaaaat ttttggtagc gaatttgcta gtttccccta tctatctttc tatggaaatg caaaagaggc gtactttgat aacacgtact acccaacgcg ttgtgggtgg attgttgaaa agttaaatac tacacaagat caattcctcc gggatggaga cgaggtgcga ctaaaacatg tttccagcgg aaagtatcta gcaacaactc ctcttaagga tacccatggt acactcacgc gtacaacgaa ctgtgaagat gctatcttta ttattaaaaa atcttcaggt tattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat