ChtrB.20272.a

Deubiquitinase and deneddylase Dub2 (ChlaDub2) (EC 3.4.22.-)

CENTER ID: ChtrB.20272.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20272.a.B1.GE42964 full length 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.958
UniProt: O84875

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPIHNPPPQ TCSYSRPSTT YTSFKDASCG TKVTRIIIAL FLIVISCGLI LCAYTFRDLL DADYSAQEGP QQATKLLQQL DKVLTGPPLP IWDNEHLFQF SCLMQNKHRR VLPIDICNPL TKFNFLEYIC NCLMTKQSVN VNETDMCELF CPPTCTPENY RRLLCTSSVF PFVMWHDPSA DTQEAMLTKM DQTMSSGRVG NSHWVLVIVD IEHRCVTFFD SFYDYIASPQ QMREQLEGLA ASLGAIYPKE GGADSDQEEL LSPFQVRIGS TVKVQSPGEF TCGAWCCQFL AWYLENPDFD LEEKVPTNPS ERRALLADFI STTEQAMSRY SSLSWPTTD
NT Sequence
atggaaccaa ttcataatcc tcccccacaa acatgttcgt attctagacc ttcaactacc tatacatctt tcaaagatgc ttcttgcggt actaaagtta ctagaatcat catagcgcta ttcctcatag tgatctcttg cggacttatt ctctgcgcat atacattccg tgacctttta gatgcggatt actcagcaca agagggacca cagcaagcaa ctaagctttt acaacaacta gataaggtct taaccggtcc tcctcttcct atctgggata acgaacattt gttccaattc tcgtgcttaa tgcagaacaa acacaggcgg gttctcccta tagacatctg taatccgctt accaaattca atttcttaga atatatttgt aactgcctca tgacgaaaca atccgttaac gtcaacgaaa ccgacatgtg cgagcttttc tgtcctccga cttgcacacc agaaaactat cgacgacttc tgtgcacctc tagtgtattc ccattcgtta tgtggcatga tccttctgct gatacgcaag aagctatgct tacgaaaatg gatcagacta tgagctctgg ccgagtagga aatagtcact gggtactggt tatcgtagat atcgagcatc gatgtgtcac attcttcgac agtttttacg actatatagc ctctccacaa caaatgcggg aacaattaga aggacttgcc gcctcccttg gagctatcta tcccaaagaa ggtggagcag actccgatca agaagaatta ctttctcctt tccaggtacg catcggatcg acagtaaaag tccaatctcc tggagaattc acctgtggag cttggtgctg tcaattcttg gcgtggtacc tagaaaatcc tgactttgat cttgaagaga aagtacctac aaacccatct gagagaagag ctctactcgc tgattttatc tctacaacgg aacaagctat gtctagatac tcatctttga gctggccaac tacggattag
Details for ChtrB.20272.a.B1.GE42964
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME PIHNPPPQTC SYSRPSTTYT SFKDASCGTK VTRIIIALFL IVISCGLILC AYTFRDLLDA DYSAQEGPQQ ATKLLQQLDK VLTGPPLPIW DNEHLFQFSC LMQNKHRRVL PIDICNPLTK FNFLEYICNC LMTKQSVNVN ETDMCELFCP PTCTPENYRR LLCTSSVFPF VMWHDPSADT QEAMLTKMDQ TMSSGRVGNS HWVLVIVDIE HRCVTFFDSF YDYIASPQQM REQLEGLAAS LGAIYPKEGG ADSDQEELLS PFQVRIGSTV KVQSPGEFTC GAWCCQFLAW YLENPDFDLE EKVPTNPSER RALLADFIST TEQAMSRYSS LSWPTTD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac caattcataa tcctccccca caaacatgtt cgtattctag accttcaact acctatacat ctttcaaaga tgcttcttgc ggtactaaag ttactagaat catcatagcg ctattcctca tagtgatctc ttgcggactt attctctgcg catatacatt ccgtgacctt ttagatgcgg attactcagc acaagaggga ccacagcaag caactaagct tttacaacaa ctagataagg tcttaaccgg tcctcctctt cctatctggg ataacgaaca tttgttccaa ttctcgtgct taatgcagaa caaacacagg cgggttctcc ctatagacat ctgtaatccg cttaccaaat tcaatttctt agaatatatt tgtaactgcc tcatgacgaa acaatccgtt aacgtcaacg aaaccgacat gtgcgagctt ttctgtcctc cgacttgcac accagaaaac tatcgacgac ttctgtgcac ctctagtgta ttcccattcg ttatgtggca tgatccttct gctgatacgc aagaagctat gcttacgaaa atggatcaga ctatgagctc tggccgagta ggaaatagtc actgggtact ggttatcgta gatatcgagc atcgatgtgt cacattcttc gacagttttt acgactatat agcctctcca caacaaatgc gggaacaatt agaaggactt gccgcctccc ttggagctat ctatcccaaa gaaggtggag cagactccga tcaagaagaa ttactttctc ctttccaggt acgcatcgga tcgacagtaa aagtccaatc tcctggagaa ttcacctgtg gagcttggtg ctgtcaattc ttggcgtggt acctagaaaa tcctgacttt gatcttgaag agaaagtacc tacaaaccca tctgagagaa gagctctact cgctgatttt atctctacaa cggaacaagc tatgtctaga tactcatctt tgagctggcc aactacggat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat