ChtrB.20264.a

Uncharacterized protein

CENTER ID: ChtrB.20264.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20264.a.B2.GE43584 soluble domain 1 114 257
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.206
UniProt: O84195

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFFSGSVETL PPSTCLISKK TRKEEFVRSV GQEASQNTLS WTIRPRIPSI IQGSKEVSLA LFVLGTVLAI VGACAAAVGG AFSVCLGVLF LGGVVLATGL LLAVLEFCHI RSSMEKYVIL TKQDLFKEPV IQEEQATPLI EEASYTCEPG IPLSGPEEVQ QERPVILQKD LDLSHAPKYI AVGSHVVELV KAGKIGRNGE RLLEEGIDTD QNFVRVWDEL FILGQRGEMV RLDGFCCKVL PKKSKSESIN NLVSNDC
NT Sequence
gtgtttttct cgggttcagt agaaactctt ccgccatcta cttgtttgat ctcaaagaaa acaagaaagg aggagtttgt gcgatcggtt ggacaagaag cttcacagaa tacattgtct tggacaattc gaccgcgaat acccagtata attcaaggta gtaaagaggt ctcgttagct ttatttgttc tgggcacagt tttggctatt gttggagcgt gtgctgcagc tgttggtgga gctttttctg tttgtttggg agttttgttt cttggtgggg ttgtattagc caccgggctt ctattagctg ttttagaatt ttgtcacatc cgaagcagca tggaaaaata cgtgattcta acaaaacagg atctgtttaa agaacctgtt atccaagaag agcaggcgac tcctcttatt gaagaagctt cttatacttg tgagccaggg attcccttgt ctggtccgga ggaagtccaa caagagcgtc cagtcattct gcagaaagat ttagatctta gtcatgctcc taaatacatc gctgtgggca gccatgtagt tgaattagtc aaagctggta aaataggccg taatggagaa cggttgttag aagaaggaat cgatacggat caaaattttg ttcgcgtttg ggatgaatta tttattctag gacaaagagg tgaaatggtt cgtttagatg gtttctgttg taaagttttg cctaagaaat ctaaaagtga gtcgattaat aatttagttt ccaatgattg ttaa
Details for ChtrB.20264.a.B2.GE43584
HARVESTED ON: 2/3/2020
SEQUENCED ON: 2/13/2020
EXPECTED MW: 17kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME KYVILTKQDL FKEPVIQEEQ ATPLIEEASY TCEPGIPLSG PEEVQQERPV ILQKDLDLSH VPKYIAVGSH VVELVKAGKI GRNGELLLEE GIDTDQNFVR VWDELFILGQ RGEVVRLDGF CCKVLPKTSK SESINNLVSN DC
Validated NT Sequence
atggctcacc accaccacca ccatatggaa aaatacgtga ttctaacaaa acaggatctg tttaaagaac ctgttatcca agaagagcag gcgactcctc ttattgaaga agcttcttat acttgtgagc cagggattcc cttgtctggt ccggaggaag tccaacaaga gcgtccagtc attctgcaga aagatttaga tcttagtcat gttcctaaat acatcgctgt gggcagccat gtagttgaac tagtcaaagc tggtaaaata ggccgtaatg gagaactgct gttagaagaa ggaatcgata cggatcaaaa ttttgttcgc gtttgggatg aattatttat tctaggacaa agaggtgaag tggttcgttt agatggtttc tgttgtaaag ttttgcctaa gacatctaaa agtgagtcga ttaataattt agtttccaat gattgttaag tgagtaagat
Expected Protein Sequence
MAHHHHHHME KYVILTKQDL FKEPVIQEEQ ATPLIEEASY TCEPGIPLSG PEEVQQERPV ILQKDLDLSH APKYIAVGSH VVELVKAGKI GRNGERLLEE GIDTDQNFVR VWDELFILGQ RGEMVRLDGF CCKVLPKKSK SESINNLVSN DC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aatacgtgat tctaacaaaa caggatctgt ttaaagaacc tgttatccaa gaagagcagg cgactcctct tattgaagaa gcttcttata cttgtgagcc agggattccc ttgtctggtc cggaggaagt ccaacaagag cgtccagtca ttctgcagaa agatttagat cttagtcatg ctcctaaata catcgctgtg ggcagccatg tagttgaatt agtcaaagct ggtaaaatag gccgtaatgg agaacggttg ttagaagaag gaatcgatac ggatcaaaat tttgttcgcg tttgggatga attatttatt ctaggacaaa gaggtgaaat ggttcgttta gatggtttct gttgtaaagt tttgcctaag aaatctaaaa gtgagtcgat taataattta gtttccaatg attgttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat