ChtrB.20260.a

Uncharacterized protein (CT135)

CENTER ID: ChtrB.20260.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20260.a.MB7.GE44055 soluble domain 2 146 210
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_2670
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272561.5.peg.147
UniProt: O84137
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSFDLNDPV RNTDNHYRNI NRMLNSATCA AGGIGLLTPV VCSPMGAFCF AQGPSSAEDL GHRIQHFVAC SGPAAGFYSL SNERIMFEEA AVPSVLEAVE ATFWISAFAR LRGNEPSTCD TVMMSCVIGC ISLVCGAMFV AIVSCAVKIS RIVRTMTQAH ALRETIQRQL AARATDMRSA YSKLKGIIAI RALNEVERGH RKLRNKMITA FVANALITLA FCALLASAVI AAFFFGAASA GLASVFFGCL WGGIGALAVG VLVGIVSGIC QRNYKVEAAR CIQRGALYAL VLEKMQRFPK EFLKDGVAKS VVAIQAGESL DTGELAWEEM PSITACLGRE GMDAQAYSFL SASPLDARIE
NT Sequence
atggtaagct tcgatttaaa tgatccagta aggaatacag acaatcatta cagaaatatc aatcgcatgc tcaatagtgc gacttgtgct gctggcggca taggattgtt aacaccagtg gtatgctctc caatgggagc tttctgtttt gctcaagggc cttctagtgc cgaagactta gggcatcgta ttcaacattt tgttgcgtgt tcgggaccag ctgcaggatt ctattctcta agtaacgaac ggatcatgtt tgaagaggct gcagttccta gtgttctcga agccgtagaa gcaacttttt ggatatctgc cttcgcccgt ttgagaggga atgaaccttc aacttgcgat actgtgatga tgagttgcgt aattggatgc atttctttgg tatgcggggc tatgtttgtt gccattgtct cctgtgctgt gaaaattagt cgcattgtga gaactatgac gcaggcacat gcgctccgtg aaacaattca aagacagtta gcagcacgag ctacagatat gcgttctgct tactccaagc tcaaaggtat tatagccata agagctctta atgaagtaga gaggggccat cgaaaattaa gaaacaaaat gatcacagct tttgttgcaa atgcactcat tacactagct ttctgtgctt tattagcttc tgcggtaatc gcagcatttt tctttggtgc agcaagtgct ggactagcaa gcgttttctt tgggtgctta tggggaggca taggagcctt agctgtcgga gttttggtcg gcatcgtttc cggaatctgc cagcgcaact ataaagtaga agctgcgcgg tgtattcagc gaggtgctct ttatgcgctt gttcttgaga aaatgcagcg attccctaaa gaattcctta aagatggtgt agcgaaaagt gttgttgcga ttcaagctgg ggaatctttg gatacaggag agttggcttg ggaagagatg ccgagcatca cagcttgttt aggaagagag ggaatggatg ctcaggcgta ttcctttctt tctgcaagtc ctttagatgc gcgtatagag taa
Details for ChtrB.20260.a.MB7.GE44055
HARVESTED ON: 1/7/2021
SEQUENCED ON: 1/8/2021
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 55
Validated AA Sequence
MNADTDYSIA EAAFXKGETA MXINGPWAWS NIDTSKVNYG VTVLPTFKXQ PSXPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQINSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSMA VKISRIVRTM TQAHALRETI QRQLAARATD MRSAYSKLKG IIAIRALNEV ERGHXKLRNK MITA
Validated NT Sequence
cnngctntgt tagcagccgg atcctcgaga agcttggctg cggaacttgt tcgtgctgtt tattaagctg tgatcatttt gtttcttaat tttcnatggc ccctctctac ttcattaaga gctcttatgg ctataatacc tttgagcttg gagtaagcag aacgcatatc tgtagctcgt gctgctaact gtctttgaat tgtttcacgg agcgcatgtg cctgcgtcat agttctcaca atgcgactaa ttttcacagc catcgaacca ggaccctggg tctgagcttc cagggtacct tctgaaatcc ttccctcgat cccgaggttg ttgttattgt tgttgttgtt gttgttcgag ctcgaattaa tctgcgcgtc tttcagggct tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac ggcataccag aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatagtg gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc tacggcaccc agcggtttgt ctttattaac cgcttccaga ccttcatcag tcagcagata gttttcgagg aactcttttg ccagctcttt gttcggactg gcggcgttaa tacctgcgct cagcacgcca acgaacggnt tggatggttg anccttgaan gtcggcagta ccgttacacc ataattcact ttgctggtgt cgatgttgga ccatgcccac gggccgttga tnnncatcgc tgtttcgcct ttantaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tntgntttta atcaggncaa cnnnnaangt canacccgct ttcgnnncag cggtatccac gcccncgtct tantgtcgta cttgccggtt tcatacttgn acgcntnaac cccgtcagnn gcatcagcnn naggtgnann ncgnnncttn canntgaaca tcnnncggct ntnncttnnc ttcnntnttt atcnnnnnng nnnntcnnnc nnnnntttgn nngnnnnnnn nnnaannntt tnnnat
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS AVKISRIVRT MTQAHALRET IQRQLAARAT DMRSAYSKLK GIIAIRALNE VERGHRKLRN KMITA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggctgt gaaaattagt cgcattgtga gaactatgac gcaggcacat gcgctccgtg aaacaattca aagacagtta gcagcacgag ctacagatat gcgttctgct tactccaagc tcaaaggtat tatagccata agagctctta atgaagtaga gaggggccat cgaaaattaa gaaacaaaat gatcacagct taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa