ChtrB.20256.a

Uncharacterized protein CT_288

CENTER ID: ChtrB.20256.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20256.a.B1.GE42952 full length 1 563
ChtrB.20256.a.B2.GE43582 soluble domain 2 287 563
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.308
UniProt: O84290

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVYFRAHQPR HTPKTFPLEV HHSFSDKHPQ IAKAMRITGI ALAALSLLAV VACVIAVSAG GAAIPLAVIS GIAVMSGLLS AATIICSAKK ALAQRKQKQL EESLPLDNAT EHVSYLTSDT SYFNQWESLG ALNKQLSQID LTIQAPEKKL LKEVLGSRYD SINHSIEEIS DRFTKMLSLL RLREHFYRGE ERYAPYLSPP LLNKNRLLTQ ITSNMIRMLP KSGGVFSLKA NTLSHASRTL YTVLKVALSL GVLAGVAALI IFLPPSLPFI AVIGVSSLAL GMASFLMIRG IKYLLEHSPL NRKQLAKDIQ KTIGPDVLAS MVHYQHQLLS HLHETLLDEA ITARWSEPFF IEHANLKAKI EDLTKQYDIL NAAFNKSLQQ DEALRSQLEK RAYLFPIPNN DENAKTKESQ LLDSENDSNS EFQEIINKGL EAANKRRADA KSKFYTEDET SDKIFSIWKP TKNLALEDLW RVHEACNEEQ QALLLEDYMS YKTSECQAAL QKVSQELKAA QKSFAVLEKH ALDRSYESSV ATMDLARANQ ETHRLLNILS ELQQLAQYLL DNH
NT Sequence
atggtttatt ttagagctca tcaacctagg catacgccta aaacatttcc tttggaagtt caccattcgt tctccgataa gcatcctcaa attgctaaag ctatgcggat tacggggata gccctcgcag ctctatctct gctcgctgta gtcgcctgcg ttattgccgt ctctgcggga ggagctgcca ttcctcttgc tgtcattagt ggaattgctg taatgtctgg cctcttatcc gctgccacca ttatctgttc tgcaaaaaag gctttggctc aacgaaaaca aaaacaacta gaagagtcgc ttccgttaga taatgcgacc gagcatgtga gttacctgac ctcagacacc tcttatttta atcaatggga atccttaggt gctctaaata agcagttgtc tcagattgac ttaactattc aagctcccga aaaaaaacta ttaaaagaag ttcttggttc cagatacgat tccattaatc actccatcga agagatctcc gatcgcttta cgaaaatgct ctctcttctt cgattaagag aacattttta tcgaggagaa gagcgttatg ccccctattt aagccctcct ctacttaaca agaatcgttt gctgacccaa atcacatcca atatgattag gatgctacca aaatccggtg gtgttttttc cctcaaagcc aatacactaa gtcatgccag ccgcacacta tatacagtat taaaagtcgc tttatcctta ggagttctcg ctggagtcgc tgctcttatc atctttcttc cccctagcct gccttttatc gctgttatag gagtatcttc cttagcattg gggatggcat ctttccttat gattcggggc attaagtatt tgctcgaaca ttctcctctg aatagaaagc aactagctaa agatattcaa aaaaccattg gcccagatgt cttggcctct atggttcatt accagcatca attactatca catctacatg aaactctatt agatgaagcc atcacagcta gatggagcga gcccttcttt attgaacacg ctaatcttaa ggcaaaaatt gaagatttga caaaacaata tgatatattg aacgcagcct ttaataaatc tttacaacaa gatgaggcgc tccgttctca attagagaaa cgagcttact tattcccaat tcctaataac gacgaaaatg ctaaaactaa agaatcgcag cttctagact cagaaaatga ttcaaattct gaatttcagg agattataaa taaaggacta gaagctgcca ataaacgacg agctgacgct aagtcaaaat tctatacgga agacgaaacc tctgacaaaa tattctctat atggaaaccc acaaagaact tggcattaga agatttgtgg agagtgcatg aagcttgcaa tgaagagcaa caagctctcc tcttagaaga ttatatgagt tataaaacct cagaatgtca agctgcactc caaaaagtga gtcaagaact gaaggcggca caaaaatcat tcgcagtcct agaaaagcat gctctagaca gatcttatga atccagtgta gccacgatgg atttagctag agcgaatcaa gaaacacacc ggcttctgaa catcctctct gaattacaac aactagcaca atacctgtta gataatcact aa
Details for ChtrB.20256.a.B1.GE42952
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMV YFRAHQPRHT PKTFPLEVHH SFSDKHPQIA KAMRITGIAL AALSLLAVVA CVIAVSAGGA AIPLAVISGI AVMSGLLSAA TIICSAKKAL AQRKQKQLEE SLPLDNATEH VSYLTSDTSY FNQWESLGAL NKQLSQIDLT IQAPEKKLLK EVLGSRYDSI NHSIEEISDR FTKMLSLLRL REHFYRGEER YAPYLSPPLL NKNRLLTQIT SNMIRMLPKS GGVFSLKANT LSHASRTLYT VLKVALSLGV LAGVAALIIF LPPSLPFIAV IGVSSLALGM ASFLMIRGIK YLLEHSPLNR KQLAKDIQKT IGPDVLASMV HYQHQLLSHL HETLLDEAIT ARWSEPFFIE HANLKAKIED LTKQYDILNA AFNKSLQQDE ALRSQLEKRA YLFPIPNNDE NAKTKESQLL DSENDSNSEF QEIINKGLEA ANKRRADAKS KFYTEDETSD KIFSIWKPTK NLALEDLWRV HEACNEEQQA LLLEDYMSYK TSECQAALQK VSQELKAAQK SFAVLEKHAL DRSYESSVAT MDLARANQET HRLLNILSEL QQLAQYLLDN H
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttt attttagagc tcatcaacct aggcatacgc ctaaaacatt tcctttggaa gttcaccatt cgttctccga taagcatcct caaattgcta aagctatgcg gattacgggg atagccctcg cagctctatc tctgctcgct gtagtcgcct gcgttattgc cgtctctgcg ggaggagctg ccattcctct tgctgtcatt agtggaattg ctgtaatgtc tggcctctta tccgctgcca ccattatctg ttctgcaaaa aaggctttgg ctcaacgaaa acaaaaacaa ctagaagagt cgcttccgtt agataatgcg accgagcatg tgagttacct gacctcagac acctcttatt ttaatcaatg ggaatcctta ggtgctctaa ataagcagtt gtctcagatt gacttaacta ttcaagctcc cgaaaaaaaa ctattaaaag aagttcttgg ttccagatac gattccatta atcactccat cgaagagatc tccgatcgct ttacgaaaat gctctctctt cttcgattaa gagaacattt ttatcgagga gaagagcgtt atgcccccta tttaagccct cctctactta acaagaatcg tttgctgacc caaatcacat ccaatatgat taggatgcta ccaaaatccg gtggtgtttt ttccctcaaa gccaatacac taagtcatgc cagccgcaca ctatatacag tattaaaagt cgctttatcc ttaggagttc tcgctggagt cgctgctctt atcatctttc ttccccctag cctgcctttt atcgctgtta taggagtatc ttccttagca ttggggatgg catctttcct tatgattcgg ggcattaagt atttgctcga acattctcct ctgaatagaa agcaactagc taaagatatt caaaaaacca ttggcccaga tgtcttggcc tctatggttc attaccagca tcaattacta tcacatctac atgaaactct attagatgaa gccatcacag ctagatggag cgagcccttc tttattgaac acgctaatct taaggcaaaa attgaagatt tgacaaaaca atatgatata ttgaacgcag cctttaataa atctttacaa caagatgagg cgctccgttc tcaattagag aaacgagctt acttattccc aattcctaat aacgacgaaa atgctaaaac taaagaatcg cagcttctag actcagaaaa tgattcaaat tctgaatttc aggagattat aaataaagga ctagaagctg ccaataaacg acgagctgac gctaagtcaa aattctatac ggaagacgaa acctctgaca aaatattctc tatatggaaa cccacaaaga acttggcatt agaagatttg tggagagtgc atgaagcttg caatgaagag caacaagctc tcctcttaga agattatatg agttataaaa cctcagaatg tcaagctgca ctccaaaaag tgagtcaaga actgaaggcg gcacaaaaat cattcgcagt cctagaaaag catgctctag acagatctta tgaatccagt gtagccacga tggatttagc tagagcgaat caagaaacac accggcttct gaacatcctc tctgaattac aacaactagc acaatacctg ttagataatc actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for ChtrB.20256.a.B2.GE43582
HARVESTED ON: data unavailable
SEQUENCED ON: 2/13/2020
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 84
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMI RGIKYLLEHS PLNRKQLAKD IQKTISPDVW ASMVHYQHQL LSHLXETLLD EAITARWGEP FFIEHANLKA KIEDLTKQYX ILNAAFNKSL QQDEALRSQV EKRXYLFPIP NNDENAKTKE SQLLDSENDS NSEFQEIINK GLXAANKRRA DAKSKFYTEX ETSDKXFSIW KPTKKLGXXR FCGECMMKLA MKATSXPLXR LYEL
Validated NT Sequence
tnnacttnnt ttngggagac cacaacggtt tccctctaga atanntttgt ttaactttnn nanngagata taccatggct caccaccacc accaccatat gattcggggc attaagtatt tgctcgaaca ttctcctctg aatagaaagc agctagctaa agatattcaa aaaaccatta gcccagatgt ctgggcctct atggttcatt accagcatca attactatca catctacntg aaactctatt agatgaagcc atcacagcta gatggggcga gcccttcttt attgaacacg ctaatcttaa ggcaaaaatt gaagatttga caaaacaata tganatattg aacgcagcct ttaataaatc tttacaacaa gatgaggcgc tccgttctca agtagagaaa cgnncttact tattcccaat tcctaataac gacgaaaatg ctaaaactaa agaatcgcag cttctagact cagaaaatga ttcaaattct gaatttcagg agattataaa taaaggacta naagctgcca ataaacgacg agctgacgct aagtcaaaat tctatactga anacgaaacc tctgacaaaa nattctctat atggaaaccc acaaagaaac ttggcnttan aagattttgt ggagagtgca tgatgaagct tgcaatgaaa gcaacaagcn ctcctcttan aagattatat gagttataaa acctcanaat gtcaagctgc actccaaaaa gtgagtcnag aactganngc ggcacaaaaa tcattcccag tcctanaaaa nntgntctan ncnnatctta tgatccataa cctgnnggat ttagctanan cgatcaagaa acacaccggn ntcctgaacn tcctnttctg aantacaann acnnnncata cctgnnaaat atcnctaann gannnanata ggatccngct gntnannaag cccnaaagga anngaatngc tgctgcnncc ncnnannnnn acctancatn ancccnnggg gcctctannn nnnntnnngn nnnnttngct gaanggngnn antnnntncn nancnnnnac ggnnnngnnn nnnnnnnnnt nnnnnnntcn nnannggnnn nnnnannnan annnnntnnn nnnnnnnnnn gnnnnnnnnn nnnntcnnna
Expected Protein Sequence
MAHHHHHHMI RGIKYLLEHS PLNRKQLAKD IQKTIGPDVL ASMVHYQHQL LSHLHETLLD EAITARWSEP FFIEHANLKA KIEDLTKQYD ILNAAFNKSL QQDEALRSQL EKRAYLFPIP NNDENAKTKE SQLLDSENDS NSEFQEIINK GLEAANKRRA DAKSKFYTED ETSDKIFSIW KPTKNLALED LWRVHEACNE EQQALLLEDY MSYKTSECQA ALQKVSQELK AAQKSFAVLE KHALDRSYES SVATMDLARA NQETHRLLNI LSELQQLAQY LLDNH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc ggggcattaa gtatttgctc gaacattctc ctctgaatag aaagcaacta gctaaagata ttcaaaaaac cattggccca gatgtcttgg cctctatggt tcattaccag catcaattac tatcacatct acatgaaact ctattagatg aagccatcac agctagatgg agcgagccct tctttattga acacgctaat cttaaggcaa aaattgaaga tttgacaaaa caatatgata tattgaacgc agcctttaat aaatctttac aacaagatga ggcgctccgt tctcaattag agaaacgagc ttacttattc ccaattccta ataacgacga aaatgctaaa actaaagaat cgcagcttct agactcagaa aatgattcaa attctgaatt tcaggagatt ataaataaag gactagaagc tgccaataaa cgacgagctg acgctaagtc aaaattctat acggaagacg aaacctctga caaaatattc tctatatgga aacccacaaa gaacttggca ttagaagatt tgtggagagt gcatgaagct tgcaatgaag agcaacaagc tctcctctta gaagattata tgagttataa aacctcagaa tgtcaagctg cactccaaaa agtgagtcaa gaactgaagg cggcacaaaa atcattcgca gtcctagaaa agcatgctct agacagatct tatgaatcca gtgtagccac gatggattta gctagagcga atcaagaaac acaccggctt ctgaacatcc tctctgaatt acaacaacta gcacaatacc tgttagataa tcactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat