ChtrB.20254.a

Uncharacterized protein

CENTER ID: ChtrB.20254.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20254.a.B1.GE42950 full length 1 270
ChtrB.20254.a.B2.GE43581 soluble domain 1 96 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.239
UniProt: O84226

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSLALGTSN GVEANNGIND LSPAPEAKKT GSGLCYKISA VAALVLGLLA AAGGAVVLAL FCTFAPPLFF YAGVALVALG AVILGVGVSN TCSCCLRSRK IEAHKQLILQ QKEEISQLEQ QLAKALKELN TKYPASLLER RDLRENLKAW QSCCLNLEKE VRDLLTKLGG YQERLKVLPA KEKQIEELKA MLEHYSRICH ERGELINLLK TANKKLSKES EKLLFNYKAH RDVCLGEKVL VKSVNLIDLD PKSDSSDGDD DDGFNYGSRV
NT Sequence
atggtgagtt tagcattagg gacttctaat ggagtagaag cgaataacgg aattaatgat ctatcgccag cacctgaagc aaaaaaaacc ggttctgggt tgtgttataa aattagtgct gtcgctgcgt tggttttagg gcttttagca gctgctggtg gtgctgttgt attggcttta ttctgtacct ttgcgccgcc tctcttcttc tatgcaggag tcgctctggt tgctttggga gctgttattt taggggtagg agtttcgaat acatgtagtt gttgcttgag atctagaaaa atagaagctc acaagcaact catccttcag caaaaagaag aaatctcaca attagagcaa caattggcta aagctctaaa agaattgaat actaagtatc ctgcaagtct tttagagaga cgtgatttac gagaaaattt aaaggcgtgg caatcttgtt gtctgaatct tgaaaaagag gtgcgagatc ttctaacgaa gctggggggg tatcaagaaa gactaaaagt cctgcctgct aaagagaaac agatagaaga gcttaaagct atgttagagc actactctcg tatttgtcac gaacgaggag agctcattaa tctgctaaaa acggctaata aaaagctatc aaaggaatct gagaagcttt tgttcaacta taaggctcat cgcgacgttt gtctcggaga aaaggttctg gtgaaatcgg ttaatctcat agacctggat ccgaaatcgg atagcagtga tggcgatgat gatgatggtt tcaattacgg ctctcgggtg tag
Details for ChtrB.20254.a.B1.GE42950
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMV SLALGTSNGV EANNGINDLS PAPEAKKTGS GLCYKISAVA ALVLGLLAAA GGAVVLALFC TFAPPLFFYA GVALVALGAV ILGVGVSNTC SCCLRSRKIE AHKQLILQQK EEISQLEQQL AKALKELNTK YPASLLERRD LRENLKAWQS CCLNLEKEVR DLLTKLGGYQ ERLKVLPAKE KQIEELKAML EHYSRICHER GELINLLKTA NKKLSKESEK LLFNYKAHRD VCLGEKVLVK SVNLIDLDPK SDSSDGDDDD GFNYGSRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga gtttagcatt agggacttct aatggagtag aagcgaataa cggaattaat gatctatcgc cagcacctga agcaaaaaaa accggttctg ggttgtgtta taaaattagt gctgtcgctg cgttggtttt agggctttta gcagctgctg gtggtgctgt tgtattggct ttattctgta cctttgcgcc gcctctcttc ttctatgcag gagtcgctct ggttgctttg ggagctgtta ttttaggggt aggagtttcg aatacatgta gttgttgctt gagatctaga aaaatagaag ctcacaagca actcatcctt cagcaaaaag aagaaatctc acaattagag caacaattgg ctaaagctct aaaagaattg aatactaagt atcctgcaag tcttttagag agacgtgatt tacgagaaaa tttaaaggcg tggcaatctt gttgtctgaa tcttgaaaaa gaggtgcgag atcttctaac gaagctgggg gggtatcaag aaagactaaa agtcctgcct gctaaagaga aacagataga agagcttaaa gctatgttag agcactactc tcgtatttgt cacgaacgag gagagctcat taatctgcta aaaacggcta ataaaaagct atcaaaggaa tctgagaagc ttttgttcaa ctataaggct catcgcgacg tttgtctcgg agaaaaggtt ctggtgaaat cggttaatct catagacctg gatccgaaat cggatagcag tgatggcgat gatgatgatg gtttcaatta cggctctcgg gtgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for ChtrB.20254.a.B2.GE43581
HARVESTED ON: data unavailable
SEQUENCED ON: 2/13/2020
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 84
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHML RSRKIEAREQ LLLQQKEEIS QLEQQLAKAL GELDTKCASL LERRDLRENL KAWQAYCLNL EEDVRDLLKK LEGYQERLKX LPAKEKQIEE LKAMLEHYSR ICYERGDLIR MLKTANKKLS KECEKLLLNY KAHLDVCLGE KGSGEIG
Validated NT Sequence
gantnnnnnt nngggagacc acnncggttt ccctctagaa ataattttgt ttaacttnna gnannngana taccatggct caccaccacc accaccatat gttgagatct agaaaaatag aagctcgcga gcaactcctc cttcagcaaa aagaagaaat ctcacaatta gagcaacaat tggctaaagc tctaggagaa ttggatacta agtgtgcaag tcttttagag agacgtgatt tacgagaaaa tttaaaggcg tggcaagctt attgtctgaa tcttgaagaa gatgtgcgag atcttctaaa gaagctggag gggtatcaag aaagactaaa agncctgcct gctaaagaga aacagataga agagctgaaa gctatgttag agcactactc tcgtatttgt tacgaacgag gagatctcat taggatgcta aaaacggcta ataaaaagct atcgaaggaa tgtgagaagc ttttgttaaa ctataaggct catctcgacg tttgtctcgg agaaaaaggt tctggcgaaa tcggttaatc tcatagacct ggatctgaaa tcggatagca gtgatggcga tgattatgat ttcanttacn gntctcnngt gtaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gantnggntg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaactg gtcttgangg gttttttgct gaaanaagga actatatccg gatatccaca ggacnggtgt ggtcgccatg atcgcgtant cgatagnggc tccaagtnag cgagcgagca tgactgggcg gcggncaaag cggtcngnac agtgctccga naacgggtgc gcatanaaat tgcntcancg catnntagcg tagcancacg ccanantgac ngtnnatgct gtcggaangg acgnnntccc ncnannnnnn cngnagtacc ggcatnancn nnccnnnnnc ttactgcatc naggngnncg gngccnagga nnacgnnnnn ncnnnttnnn nnnntcnana nnnnnnngan nnnnnnnnna ntttnnnnnn gnnanaannn nnnnnatnan gctnnnnnnn ngnnnnnnnn nnnnnnngna naannnntnn aanannangn nncncggnnn cnnnncnnnt ttntttnnnn gg
Expected Protein Sequence
MAHHHHHHLR SRKIEAHKQL ILQQKEEISQ LEQQLAKALK ELNTKYPASL LERRDLRENL KAWQSCCLNL EKEVRDLLTK LGGYQERLKV LPAKEKQIEE LKAMLEHYSR ICHERGELIN LLKTANKKLS KESEKLLFNY KAHRDVCLGE KVLVKSVNLI DLDPKSDSSD GDDDDGFNYG SRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgagat ctagaaaaat agaagctcac aagcaactca tccttcagca aaaagaagaa atctcacaat tagagcaaca attggctaaa gctctaaaag aattgaatac taagtatcct gcaagtcttt tagagagacg tgatttacga gaaaatttaa aggcgtggca atcttgttgt ctgaatcttg aaaaagaggt gcgagatctt ctaacgaagc tgggggggta tcaagaaaga ctaaaagtcc tgcctgctaa agagaaacag atagaagagc ttaaagctat gttagagcac tactctcgta tttgtcacga acgaggagag ctcattaatc tgctaaaaac ggctaataaa aagctatcaa aggaatctga gaagcttttg ttcaactata aggctcatcg cgacgtttgt ctcggagaaa aggttctggt gaaatcggtt aatctcatag acctggatcc gaaatcggat agcagtgatg gcgatgatga tgatggtttc aattacggct ctcgggtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat