SSGCID: Target Page

ChtrB.20252.a

Uncharacterized protein

CENTER ID:	ChtrB.20252.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.20252.a.B1.GE42948	full length		1	547
ChtrB.20252.a.B2.GE43580	soluble domain 1		95	547

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.20252.a.B2.PW38860	soluble domain 1		95	547

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272561.5.peg.229
UniProt:	O84216

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTDSLFNPP DSTRGVFQFL ETQCDRAVAR SRQSQFIGLV SAVAAAALLL LLVVALSVPG FPVAASIVVG VLFALSIVAL TASFLVYIAN AKLVAIRIKF LSSGLQDHFS ESSILGTLRK GRGASIPLIS GQADDPLPNR IGIKKSTEMR VLQKGIGTDY KKYKQHLDRV NNDFTFVCEG ISALIPTEKD APFPIEPSHL AGVFLVSFSP DKNPILKITR HAEKMLQPPQ GGFPNGLVWL CGALSDPKKF AAPFLSLIEK THQGILVSKD LKDNKERKLA LEASLLSLNI FFSGWCLGNP EYNQYITTAV AEKYRDVSVR NCIYDFLDTG NVISALALAS SYSQDSAWAA GLQKVLREED KKTKKKSREE VSCLYRDIDP GCCLRALPKR FESKSSGSQG SPKEQLSSLL KALDQKIPSG ILGLIAKASS ADLKADFAGM LEVIKQLQAL FDSYPPLCED NILLWLSASL EQVGLQKKLR TFLPSSEKKL LERVLSTFLL GLYTRGVFSV GQVNQLATIC NTQDSTEFCQ RVSDLSLIKR ALPALFG

NT Sequence

atgcgaacag actctctttt caatcctccc gactctacta gaggagtttt tcagttttta gagactcagt gtgatcgagc cgtggctcgg tccagacaaa gccaatttat agggttagtc tctgctgtag cagctgcagc attattattg ttgcttgtgg tcgctctatc tgttccagga ttcccagttg cagcttcaat tgttgtaggg gttctctttg ctttatcgat cgtagcatta acagcttcgt ttttggtata tatagctaat gctaagcttg ttgcaataag aattaaattc ttgagtagtg gtctgcaaga tcacttttcg gagtcatcta ttttagggac tctccgtaaa ggacgtggtg ctagtattcc gcttatttcc ggacaagcag atgatcctct ccctaatcgg attgggatca aaaaaagcac tgaaatgcgt gttcttcaaa aaggaattgg gacagattat aaaaaatata agcagcatct tgatagagtg aataatgatt tcacttttgt ctgtgagggg attagcgctt taattcctac agaaaaagat gctccattcc ctatagaacc ttctcattta gcaggtgttt ttttagtatc attttcacca gacaagaatc cgattctaaa gattacgcgt catgctgaga agatgttaca gcctcctcaa ggcggattcc ctaacgggct ggtttggttg tgtggagctc tttctgatcc taagaaattt gcagctccct ttctatcttt gattgagaag actcaccaag ggattttggt gagtaaagac ttgaaagaca ataaggaaag aaagctagct ttagaggctt cccttctttc attgaatatt ttcttttccg gttggtgttt ggggaatccg gagtacaatc agtatatcac aactgctgta gctgagaaat atagggatgt ctctgtaaga aattgtattt atgatttcct ggatacaggg aatgtgattt cagctcttgc tttagcaagt agttattcac aagattccgc ttgggctgca gggttgcaga aagttttacg tgaagaagat aaaaagacta agaaaaagtc acgtgaagaa gtctcttgtt tgtatcgtga tatagatcca ggctgttgtt taagagccct tcctaagcga tttgaatcca agtcttcagg tagtcaaggt agtcctaaag agcagttaag ctctttgttg aaagctttag accagaaaat tccttcaggg attttaggat tgattgcaaa agcttcttct gcagatctca aggctgattt tgcaggtatg cttgaagtta ttaagcaatt acaagcttta ttcgattctt acccaccttt atgcgaagac aatattctct tgtggttaag cgcttcttta gaacaagtag gcttgcagaa gaaattgaga acctttttac cttcatcaga aaaaaaactc ttagaaagag ttctctctac atttttatta ggtttgtata ctcgaggagt cttttctgta gggcaagtga atcagctagc tactatttgt aatactcagg actctacaga attctgccag agagtaagtg acctttcgtt aattaaacga gctctacctg cattatttgg ttaa

Details for ChtrB.20252.a.B1.GE42948

HARVESTED ON:	3/4/2019
SEQUENCED ON:	3/12/2019
EXPECTED MW:	61kDa
OBSERVED MW:	61kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR TDSLFNPPDS TRGVFQFLET QCDRAVARSR QSQFIGLVSA VAAAALLLLL VVALSVPGFP VAASIVVGVL FALSIVALTA SFLVYIANAK LVAIRIKFLS SGLQDHFSES SILGTLRKGR GASIPLISGQ ADDPLPNRIG IKKSTEMRVL QKGIGTDYKK YKQHLDRVNN DFTFVCEGIS ALIPTEKDAP FPIEPSHLAG VFLVSFSPDK NPILKITRHA EKMLQPPQGG FPNGLVWLCG ALSDPKKFAA PFLSLIEKTH QGILVSKDLK DNKERKLALE ASLLSLNIFF SGWCLGNPEY NQYITTAVAE KYRDVSVRNC IYDFLDTGNV ISALALASSY SQDSAWAAGL QKVLREEDKK TKKKSREEVS CLYRDIDPGC CLRALPKRFE SKSSGSQGSP KEQLSSLLKA LDQKIPSGIL GLIAKASSAD LKADFAGMLE VIKQLQALFD SYPPLCEDNI LLWLSASLEQ VGLQKKLRTF LPSSEKKLLE RVLSTFLLGL YTRGVFSVGQ VNQLATICNT QDSTEFCQRV SDLSLIKRAL PALFG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa cagactctct tttcaatcct cccgactcta ctagaggagt ttttcagttt ttagagactc agtgtgatcg agccgtggct cggtccagac aaagccaatt tatagggtta gtctctgctg tagcagctgc agcattatta ttgttgcttg tggtcgctct atctgttcca ggattcccag ttgcagcttc aattgttgta ggggttctct ttgctttatc gatcgtagca ttaacagctt cgtttttggt atatatagct aatgctaagc ttgttgcaat aagaattaaa ttcttgagta gtggtctgca agatcacttt tcggagtcat ctattttagg gactctccgt aaaggacgtg gtgctagtat tccgcttatt tccggacaag cagatgatcc tctccctaat cggattggga tcaaaaaaag cactgaaatg cgtgttcttc aaaaaggaat tgggacagat tataaaaaat ataagcagca tcttgataga gtgaataatg atttcacttt tgtctgtgag gggattagcg ctttaattcc tacagaaaaa gatgctccat tccctataga accttctcat ttagcaggtg tttttttagt atcattttca ccagacaaga atccgattct aaagattacg cgtcatgctg agaagatgtt acagcctcct caaggcggat tccctaacgg gctggtttgg ttgtgtggag ctctttctga tcctaagaaa tttgcagctc cctttctatc tttgattgag aagactcacc aagggatttt ggtgagtaaa gacttgaaag acaataagga aagaaagcta gctttagagg cttcccttct ttcattgaat attttctttt ccggttggtg tttggggaat ccggagtaca atcagtatat cacaactgct gtagctgaga aatataggga tgtctctgta agaaattgta tttatgattt cctggataca gggaatgtga tttcagctct tgctttagca agtagttatt cacaagattc cgcttgggct gcagggttgc agaaagtttt acgtgaagaa gataaaaaga ctaagaaaaa gtcacgtgaa gaagtctctt gtttgtatcg tgatatagat ccaggctgtt gtttaagagc ccttcctaag cgatttgaat ccaagtcttc aggtagtcaa ggtagtccta aagagcagtt aagctctttg ttgaaagctt tagaccagaa aattccttca gggattttag gattgattgc aaaagcttct tctgcagatc tcaaggctga ttttgcaggt atgcttgaag ttattaagca attacaagct ttattcgatt cttacccacc tttatgcgaa gacaatattc tcttgtggtt aagcgcttct ttagaacaag taggcttgca gaagaaattg agaacctttt taccttcatc agaaaaaaaa ctcttagaaa gagttctctc tacattttta ttaggtttgt atactcgagg agtcttttct gtagggcaag tgaatcagct agctactatt tgtaatactc aggactctac agaattctgc cagagagtaa gtgacctttc gttaattaaa cgagctctac ctgcattatt tggttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ChtrB.20252.a.B2.GE43580

HARVESTED ON:	2/3/2020
SEQUENCED ON:	2/13/2020
EXPECTED MW:	51kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHAI RIKFLSSGLQ DHFSESSILG TLRKGRGASI PLISGQADDP LPNRIGIKKS TEMRVLQKGI GTDYKKYKQH LDRVNNDFTF VCEGISALIP TEKDAPFPIE PSHLAGVFLV SFSPDKNPIL KITRHAEKML QPPQGGFPNG LVWLCGALSD PKKFAAPFLS LIEKTHQGIL VSKDLKDNKE RKLALEASLL SLNIFFSGWC LGNPEYNQYI TTAVAEKYRD VSVRNCIYDF LDTGNVISAL ALASSYSQDS AWAAGLQKVL REEDKKTKKK SREEVSCLYR DIDPGCCLRA LPKRFESKSS GSQGSPKEQL SSLLKALDQK IPSGILGLIA KASSADLKAD FAGMLEVIKQ LQALFDSYPP LCEDNILLWL SASLEQVGLQ KKLRTFLPSS EKKLLERVLS TFLLGLYTRG VFSVGQVNQL ATICNTQDST EFCQRVSDLS LIKRALPALF G

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaataa gaattaaatt cttgagtagt ggtctgcaag atcacttttc ggagtcatct attttaggga ctctccgtaa aggacgtggt gctagtattc cgcttatttc cggacaagca gatgatcctc tccctaatcg gattgggatc aaaaaaagca ctgaaatgcg tgttcttcaa aaaggaattg ggacagatta taaaaaatat aagcagcatc ttgatagagt gaataatgat ttcacttttg tctgtgaggg gattagcgct ttaattccta cagaaaaaga tgctccattc cctatagaac cttctcattt agcaggtgtt tttttagtat cattttcacc agacaagaat ccgattctaa agattacgcg tcatgctgag aagatgttac agcctcctca aggcggattc cctaacgggc tggtttggtt gtgtggagct ctttctgatc ctaagaaatt tgcagctccc tttctatctt tgattgagaa gactcaccaa gggattttgg tgagtaaaga cttgaaagac aataaggaaa gaaagctagc tttagaggct tcccttcttt cattgaatat tttcttttcc ggttggtgtt tggggaatcc ggagtacaat cagtatatca caactgctgt agctgagaaa tatagggatg tctctgtaag aaattgtatt tatgatttcc tggatacagg gaatgtgatt tcagctcttg ctttagcaag tagttattca caagattccg cttgggctgc agggttgcag aaagttttac gtgaagaaga taaaaagact aagaaaaagt cacgtgaaga agtctcttgt ttgtatcgtg atatagatcc aggctgttgt ttaagagccc ttcctaagcg atttgaatcc aagtcttcag gtagtcaagg tagtcctaaa gagcagttaa gctctttgtt gaaagcttta gaccagaaaa ttccttcagg gattttagga ttgattgcaa aagcttcttc tgcagatctc aaggctgatt ttgcaggtat gcttgaagtt attaagcaat tacaagcttt attcgattct tacccacctt tatgcgaaga caatattctc ttgtggttaa gcgcttcttt agaacaagta ggcttgcaga agaaattgag aaccttttta ccttcatcag aaaaaaaact cttagaaaga gttctctcta catttttatt aggtttgtat actcgaggag tcttttctgt agggcaagtg aatcagctag ctactatttg taatactcag gactctacag aattctgcca gagagtaagt gacctttcgt taattaaacg agctctacct gcattatttg gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for ChtrB.20252.a.B2.PW38860

PURIFICATION DATe:	6/26/2020
CONCENTRATION:	12.3mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	110
VIAL VOLUME:	5µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

running low on gel and SEC.

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAATAA GAATTAAATT CTTGAGTAGT GGTCTGCAAG ATCACTTTTC GGAGTCATCT ATTTTAGGGA CTCTCCGTAA AGGACGTGGT GCTAGTATTC CGCTTATTTC CGGACAAGCA GATGATCCTC TCCCTAATCG GATTGGGATC AAAAAAAGCA CTGAAATGCG TGTTCTTCAA AAAGGAATTG GGACAGATTA TAAAAAATAT AAGCAGCATC TTGATAGAGT GAATAATGAT TTCACTTTTG TCTGTGAGGG GATTAGCGCT TTAATTCCTA CAGAAAAAGA TGCTCCATTC CCTATAGAAC CTTCTCATTT AGCAGGTGTT TTTTTAGTAT CATTTTCACC AGACAAGAAT CCGATTCTAA AGATTACGCG TCATGCTGAG AAGATGTTAC AGCCTCCTCA AGGCGGATTC CCTAACGGGC TGGTTTGGTT GTGTGGAGCT CTTTCTGATC CTAAGAAATT TGCAGCTCCC TTTCTATCTT TGATTGAGAA GACTCACCAA GGGATTTTGG TGAGTAAAGA CTTGAAAGAC AATAAGGAAA GAAAGCTAGC TTTAGAGGCT TCCCTTCTTT CATTGAATAT TTTCTTTTCC GGTTGGTGTT TGGGGAATCC GGAGTACAAT CAGTATATCA CAACTGCTGT AGCTGAGAAA TATAGGGATG TCTCTGTAAG AAATTGTATT TATGATTTCC TGGATACAGG GAATGTGATT TCAGCTCTTG CTTTAGCAAG TAGTTATTCA CAAGATTCCG CTTGGGCTGC AGGGTTGCAG AAAGTTTTAC GTGAAGAAGA TAAAAAGACT AAGAAAAAGT CACGTGAAGA AGTCTCTTGT TTGTATCGTG ATATAGATCC AGGCTGTTGT TTAAGAGCCC TTCCTAAGCG ATTTGAATCC AAGTCTTCAG GTAGTCAAGG TAGTCCTAAA GAGCAGTTAA GCTCTTTGTT GAAAGCTTTA GACCAGAAAA TTCCTTCAGG GATTTTAGGA TTGATTGCAA AAGCTTCTTC TGCAGATCTC AAGGCTGATT TTGCAGGTAT GCTTGAAGTT ATTAAGCAAT TACAAGCTTT ATTCGATTCT TACCCACCTT TATGCGAAGA CAATATTCTC TTGTGGTTAA GCGCTTCTTT AGAACAAGTA GGCTTGCAGA AGAAATTGAG AACCTTTTTA CCTTCATCAG AAAAAAAACT CTTAGAAAGA GTTCTCTCTA CATTTTTATT AGGTTTGTAT ACTCGAGGAG TCTTTTCTGT AGGGCAAGTG AATCAGCTAG CTACTATTTG TAATACTCAG GACTCTACAG AATTCTGCCA GAGAGTAAGT GACCTTTCGT TAATTAAACG AGCTCTACCT GCATTATTTG GTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT