SSGCID: Target Page

ChtrB.20243.b

Effector from type III secretion system family protein (CT_621)

CENTER ID:	ChtrB.20243.b
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.20243.b.B1.GE42940	full length		1	832

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272561.5.peg.678
UniProt:	O84626

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNRIHRTQGS LTDYNSTLEA IAKKIAKPDS ATIVSQVAQY EQFKMEQEAL KALLVSFDQK ADQRYRNLIQ RLEQLDVDRQ TGRSTESQHI QEKPMASLQS ENQVVAQAVV QSDSSMPIFT GIKQSWAVRL VQGIREILDQ VLVDTSLFTE EERGDLLAIR MDAASLQDKQ ERLSTEDIRS LLSLSNDVMR VLQKASVSST RQLELIQSLI DIFGTEENLE QSFAQVRLEN FQAILSVIKE RLTEEEFRVF QEVSEEISSI QRTSESHLSP EHIEAIARVG GHLSAKIVES ELKASQKVDL CQRIAAMYQE QVDAVQAYHS LEQDALFVNS RQHSHFVQVI SLVSSLMHSL SPTSEEERIL LNPAMMVSVL PTVRAIGLRF DFLTAEQQQM VNAAVSSLQQ QQLDEFLGVL CAHLVVVNCQ NKETGLLEGL EESFSETLSG LSNNFVLTAK MQDILQVCSL QGFVTLANGD RYELFSYNDS GEAVCDEIAL GDGFHKVLGT MLAVALSQAE VFKQECDRFI LQADSEKNMI HKRMVQGEQK SLFLTKMQTE LNAGKTIAQT KEVEASPLPS AVASVLIDHY MPKEVEFLEK ISSRLYYGNK GSDIGNTILD AISLYVNSAT YFGFANYIGQ PPVVGKTREN IFAGSADNAK AKLDEEKKQV DVFLEITEAA KTTVTNQQSA VTNDDKLSTE QKAKIKAELT QYTDMLNAIS NSLTSLKTQL APLSVSTVEG VDGVFEVKNG IPGENGKNWR LVLQTLEDTV VSGEVGSPTN IGMFQMQALV HLNQQAYADM GQNFQLELQM HLTSMQQEWM VVATSLQLLN QIYLGLARNL LR

NT Sequence

atgaaccgta ttcatcgtac acaaggatct ttaacggatt ataatagcac tcttgaggct attgcaaaga agattgccaa accagattct gcaacaatag tatctcaggt tgctcaatat gagcagttca aaatggaaca agaagcttta aaagcacttc ttgtttcctt cgatcaaaaa gcagaccagc gatatcgaaa tttgattcaa cgactggaac agttggatgt agatagacaa acaggacgct ctacagagag ccaacacatc caagaaaaac cgatggcttc tctgcaatct gaaaaccagg ttgttgcaca agctgtagtt cagtctgatt ctagcatgcc catttttaca ggtattaaac aaagttgggc agtacgatta gtacagggta tacgtgaaat tttggatcaa gtcttagtgg acacatcctt gtttacggaa gaagaacggg gagatttact tgctatccgt atggatgccg cttccctaca ggacaagcaa gagcgtttgt ctacggaaga tattcgttct cttctttctc tttcgaatga tgttatgcga gttttgcaga aagcttctgt atctagcacg cggcagctag aattgatcca atctttgata gatatttttg gtacggaaga gaatttagag cagtccttcg cccaggtacg attggagaac ttccaagcga ttttatctgt gattaaagag cgtttgacag aagaagagtt tcgggtattc caagaagtct cggaagaaat ttcctctatc caacgcacga gtgaatctca tctcagtcca gaacatatag aggcgattgc tcgagtcggt gggcatcttt ctgctaagat cgtggagtct gagctgaagg caagtcagaa ggtggatctc tgtcaacgaa tagcagctat gtaccaagaa caagtggatg cggtacaagc gtatcatagt ctcgaacaag atgctctatt tgtgaattct aggcaacata gtcacttcgt gcaggtgatt tctttagtat cctctcttat gcactcattg tctcctacta gtgaagagga gcgtatttta ctgaaccctg ccatgatggt aagtgttctt cccactgtgc gtgcaatagg tttacgtttt gactttttaa cagctgagca gcagcaaatg gtgaatgctg cggtatcatc gttgcaacaa cagcagttag acgaattttt aggagttctc tgtgcgcatt tggttgttgt taattgccag aataaagaaa caggtctctt agaaggttta gaagaaagct tttcagaaac gttaagtggt ttgagtaata actttgtttt gacggccaaa atgcaagaca ttttgcaggt ttgttcttta caaggctttg tcacattagc aaacggggat cgatacgagt tattttccta taatgattca ggagaagcgg tatgtgatga gatcgctcta ggcgatggat ttcataaagt gctaggaacg atgttagcag tagcgctttc tcaagcagag gtatttaagc aagagtgcga tcgattcatt ctgcaagctg attcagagaa gaatatgatt cacaaacgta tggttcaagg ggaacagaag agtctattct tgaccaagat gcagacagag ttgaatgctg ggaaaactat tgcacaaact aaggaagtag aggcttctcc gctcccttct gccgttgctt ctgtattgat tgaccactac atgccaaagg aagtggagtt tttagaaaag atctcgtctc gtctgtatta cggaaataaa ggttcggata taggcaacac cattttagat gcgattagtt tgtatgtaaa ctccgcaacc tactttggtt ttgctaacta tattgggcaa cctcctgttg taggtaaaac aagggagaat atttttgcag ggtctgcaga taatgctaaa gccaagttag acgaagaaaa gaaacaagta gatgtgtttc tagaaattac ggaagcagct aagactaccg tcactaatca acaaagtgcg gttactaatg atgacaagtt atctacagag cagaaagcaa aaattaaggc agagttgaca cagtataccg atatgcttaa cgcaatcagc aattctttaa catcattgaa aactcaattg gcccctttat cagtgagtac tgttgaaggt gtggatggtg tttttgaggt taaaaatggt ataccaggag aaaacggaaa gaactggaga ttagttttac agactttaga ggatactgtg gtatccggag aagtcggtag cccaactaat attggaatgt tccaaatgca agctctggta cacttgaatc agcaggccta tgctgatatg ggacagaatt ttcagttaga gttgcaaatg catctcactt ctatgcaaca ggagtggatg gtggtagcaa catctcttca gctattaaat caaatttatt tgggattagc gcgtaatctc ttaagatag

Details for ChtrB.20243.b.B1.GE42940

HARVESTED ON:	3/4/2019
SEQUENCED ON:	3/12/2019
EXPECTED MW:	94kDa
OBSERVED MW:	94kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN RIHRTQGSLT DYNSTLEAIA KKIAKPDSAT IVSQVAQYEQ FKMEQEALKA LLVSFDQKAD QRYRNLIQRL EQLDVDRQTG RSTESQHIQE KPMASLQSEN QVVAQAVVQS DSSMPIFTGI KQSWAVRLVQ GIREILDQVL VDTSLFTEEE RGDLLAIRMD AASLQDKQER LSTEDIRSLL SLSNDVMRVL QKASVSSTRQ LELIQSLIDI FGTEENLEQS FAQVRLENFQ AILSVIKERL TEEEFRVFQE VSEEISSIQR TSESHLSPEH IEAIARVGGH LSAKIVESEL KASQKVDLCQ RIAAMYQEQV DAVQAYHSLE QDALFVNSRQ HSHFVQVISL VSSLMHSLSP TSEEERILLN PAMMVSVLPT VRAIGLRFDF LTAEQQQMVN AAVSSLQQQQ LDEFLGVLCA HLVVVNCQNK ETGLLEGLEE SFSETLSGLS NNFVLTAKMQ DILQVCSLQG FVTLANGDRY ELFSYNDSGE AVCDEIALGD GFHKVLGTML AVALSQAEVF KQECDRFILQ ADSEKNMIHK RMVQGEQKSL FLTKMQTELN AGKTIAQTKE VEASPLPSAV ASVLIDHYMP KEVEFLEKIS SRLYYGNKGS DIGNTILDAI SLYVNSATYF GFANYIGQPP VVGKTRENIF AGSADNAKAK LDEEKKQVDV FLEITEAAKT TVTNQQSAVT NDDKLSTEQK AKIKAELTQY TDMLNAISNS LTSLKTQLAP LSVSTVEGVD GVFEVKNGIP GENGKNWRLV LQTLEDTVVS GEVGSPTNIG MFQMQALVHL NQQAYADMGQ NFQLELQMHL TSMQQEWMVV ATSLQLLNQI YLGLARNLLR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gtattcatcg tacacaagga tctttaacgg attataatag cactcttgag gctattgcaa agaagattgc caaaccagat tctgcaacaa tagtatctca ggttgctcaa tatgagcagt tcaaaatgga acaagaagct ttaaaagcac ttcttgtttc cttcgatcaa aaagcagacc agcgatatcg aaatttgatt caacgactgg aacagttgga tgtagataga caaacaggac gctctacaga gagccaacac atccaagaaa aaccgatggc ttctctgcaa tctgaaaacc aggttgttgc acaagctgta gttcagtctg attctagcat gcccattttt acaggtatta aacaaagttg ggcagtacga ttagtacagg gtatacgtga aattttggat caagtcttag tggacacatc cttgtttacg gaagaagaac ggggagattt acttgctatc cgtatggatg ccgcttccct acaggacaag caagagcgtt tgtctacgga agatattcgt tctcttcttt ctctttcgaa tgatgttatg cgagttttgc agaaagcttc tgtatctagc acgcggcagc tagaattgat ccaatctttg atagatattt ttggtacgga agagaattta gagcagtcct tcgcccaggt acgattggag aacttccaag cgattttatc tgtgattaaa gagcgtttga cagaagaaga gtttcgggta ttccaagaag tctcggaaga aatttcctct atccaacgca cgagtgaatc tcatctcagt ccagaacata tagaggcgat tgctcgagtc ggtgggcatc tttctgctaa gatcgtggag tctgagctga aggcaagtca gaaggtggat ctctgtcaac gaatagcagc tatgtaccaa gaacaagtgg atgcggtaca agcgtatcat agtctcgaac aagatgctct atttgtgaat tctaggcaac atagtcactt cgtgcaggtg atttctttag tatcctctct tatgcactca ttgtctccta ctagtgaaga ggagcgtatt ttactgaacc ctgccatgat ggtaagtgtt cttcccactg tgcgtgcaat aggtttacgt tttgactttt taacagctga gcagcagcaa atggtgaatg ctgcggtatc atcgttgcaa caacagcagt tagacgaatt tttaggagtt ctctgtgcgc atttggttgt tgttaattgc cagaataaag aaacaggtct cttagaaggt ttagaagaaa gcttttcaga aacgttaagt ggtttgagta ataactttgt tttgacggcc aaaatgcaag acattttgca ggtttgttct ttacaaggct ttgtcacatt agcaaacggg gatcgatacg agttattttc ctataatgat tcaggagaag cggtatgtga tgagatcgct ctaggcgatg gatttcataa agtgctagga acgatgttag cagtagcgct ttctcaagca gaggtattta agcaagagtg cgatcgattc attctgcaag ctgattcaga gaagaatatg attcacaaac gtatggttca aggggaacag aagagtctat tcttgaccaa gatgcagaca gagttgaatg ctgggaaaac tattgcacaa actaaggaag tagaggcttc tccgctccct tctgccgttg cttctgtatt gattgaccac tacatgccaa aggaagtgga gtttttagaa aagatctcgt ctcgtctgta ttacggaaat aaaggttcgg atataggcaa caccatttta gatgcgatta gtttgtatgt aaactccgca acctactttg gttttgctaa ctatattggg caacctcctg ttgtaggtaa aacaagggag aatatttttg cagggtctgc agataatgct aaagccaagt tagacgaaga aaagaaacaa gtagatgtgt ttctagaaat tacggaagca gctaagacta ccgtcactaa tcaacaaagt gcggttacta atgatgacaa gttatctaca gagcagaaag caaaaattaa ggcagagttg acacagtata ccgatatgct taacgcaatc agcaattctt taacatcatt gaaaactcaa ttggcccctt tatcagtgag tactgttgaa ggtgtggatg gtgtttttga ggttaaaaat ggtataccag gagaaaacgg aaagaactgg agattagttt tacagacttt agaggatact gtggtatccg gagaagtcgg tagcccaact aatattggaa tgttccaaat gcaagctctg gtacacttga atcagcaggc ctatgctgat atgggacaga attttcagtt agagttgcaa atgcatctca cttctatgca acaggagtgg atggtggtag caacatctct tcagctatta aatcaaattt atttgggatt agcgcgtaat ctcttaagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat