ChtrB.20243.a

Effector from type III secretion system family protein (CT_711)

CENTER ID: ChtrB.20243.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20243.a.B1.GE42939 full length 1 767
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.20243.a.B1.PW38629 full length 1 767

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.783
UniProt: O84717

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIQPTSISL TKNITAALAG EQVDAAAVYM PQAVFFFQQL DEKSKGLKQA LGLLEEVDLE KFIPSLEKSP TPITTGTTSK ISADGIEIVG ELSSETILAD PNKAAAQVFG EGLADSFDDW LRLSENGGIQ DPTAIEEEIV TKYQTELNTL RNKLKQQSLT DDEYTKLYAI PQNFVKEIES LKNENNVRLI PKSKVTNFWQ NIMLTYNSVT SLSEPVTDAM NTTMAEYSLY IERATEAAKL IREITNTIKD IFNPVWDVRE QTGIFGLKGA EYNALEGNMI QSLLSFAGLF RQLMSRTATV DEIGALYPKN DKNEDVIHTA IDDYVNSLAD LKANEQVKLN GLLSLVYAYY ASTLGFAKKD VFNNAQASFT DYTNFLNQEI QYWTPRETSS FNISNQALQT FKNKPSADYN GVYLFDNKGL ETNLFNPTFF FDVVSLMTAD PTKTMSRQDY NKVITASESS IQKINQAITA WELAIAECGT KKAKLEPSSL NYFNAMVEAK KTFVETSPIQ MVYSSLMLDK YLPNQQYILE TLGSQMTFSN KAARYLNDII AYAVSFQTAD VYYSLGMYLR QMNQQEFPEV ISRANDTVKK EIDRSRADLF HCKKAIEKIK ELVTSVNADT ELTSSQRAEL LETLASYAFE FENLYHNLSN VYVMVSKVQI SGVSKPDEVD EAFTAKIGSK EFDTWIQQLT TFESAVIEGG RNGVMPGGEQ QVLQSLESKQ QDYTSFNQNQ QLALQMESAA IQQEWTMVAA ALALMNQIFA KLIRRFK
NT Sequence
gtgtcaatac aacctacatc catttcttta actaagaata taacggcagc tttagccgga gagcaggtcg atgctgctgc agtgtatatg ccgcaggctg tttttttctt tcagcaactg gatgaaaaaa gcaaggggct gaaacaggct ttaggattgc tcgaagaggt tgatctagaa aaatttatac cgtctttaga aaaatcacct acacctatca ctacgggaac aacgagtaaa atttccgctg atgggattga gattgttgga gagctttctt cagaaacaat tttggcagat cctaataaag ctgcagctca ggtttttgga gaggggcttg cagatagttt tgatgattgg ctcagattat ctgaaaatgg ggggattcaa gatcctacag caatagaaga agagattgtt actaagtatc aaacagaact caatactctg cgcaataaac tcaagcaaca atctttaaca gacgatgagt atacgaagct ttatgctatt cctcaaaact ttgttaaaga gatagaaagc ttaaagaatg aaaataatgt gaggttaatt cccaaaagta aagtcactaa cttttggcag aatatcatgc tcacttacaa ctcggtaacc tcgttatcag aacctgttac cgatgcgatg aatacgacta tggcggagta ctctctttat attgagagag ctacagaggc tgccaagttg atacgggaga taaccaacac gatcaaagac attttcaatc cagtttggga tgtgcgtgaa caaacaggaa tttttgggtt aaaaggagct gagtataacg ctttagaagg caatatgatt caaagcttgc ttagctttgc gggtctattc cggcagttaa tgagtcgtac tgcaacagtt gatgagatag gcgcacttta tcctaaaaat gataaaaacg aagacgtcat tcatactgct attgatgatt atgtgaattc tttagctgat ttgaaagcca atgaacaggt caaactcaac ggtctgttga gtttagtata tgcttattat gctagtactt taggttttgc taagaaggat gtattcaata atgcacaagc ttcttttaca gattatacta attttctaaa ccaagagatc caatattgga cgcctagaga gacttcaagt tttaatatct ccaatcaagc attgcaaacc tttaaaaata agccttcggc tgattataac ggcgtatatc tttttgataa taaaggatta gagactaatc tctttaatcc tacgttcttc tttgatgttg tgagtctcat gacagctgat cctacgaaga ctatgtctcg acaggattac aataaggtga ttacagcctc ggaatccagt attcagaaga ttaatcaggc tattaccgct tgggaactag ctattgcaga atgtgggact aaaaaagcga agctcgaacc atccagttta aattatttta atgctatggt cgaagcgaag aagaccttcg tagagacctc tccaatacag atggtctatt catctttgat gttggataag tatcttccga atcagcagta catattagag acattaggaa gtcagatgac tttctctaac aaggctgctc ggtatttaaa tgatatcatt gcgtatgcag ttagcttcca aacagctgac gtctattatt ctttagggat gtatcttcga caaatgaacc agcaggaatt tcctgaggtg atttctcgtg ctaacgatac tgtgaaaaaa gagatagatc ggagtcgtgc ggatctcttt cactgtaaaa aagctatcga aaagattaaa gaattagtga cttctgtaaa tgcggatact gaattgacct catctcagcg tgcagagtta ttagagacgt tagctagtta tgcttttgaa tttgagaatc tctatcacaa cctctctaat gtttacgtca tggtttctaa ggtacagatt tctggcgtaa gcaagcctga tgaagtggat gaggctttta ctgctaagat tggatcgaag gaattcgata cttggattca gcagcttaca acatttgaaa gtgctgtgat tgaaggtggg cgtaatggtg tgatgcctgg gggagagcag caggttttac agagtttaga gagcaagcag caagattaca cgtcgttcaa ccagaatcag caattagctc tacaaatgga gtccgcagcg attcaacaag agtggactat ggtagcagca gccttagcat taatgaatca gatttttgct aagttgatcc gtagatttaa ataa
Details for ChtrB.20243.a.B1.GE42939
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS IQPTSISLTK NITAALAGEQ VDAAAVYMPQ AVFFFQQLDE KSKGLKQALG LLEEVDLEKF IPSLEKSPTP ITTGTTSKIS ADGIEIVGEL SSETILADPN KAAAQVFGEG LADSFDDWLR LSENGGIQDP TAIEEEIVTK YQTELNTLRN KLKQQSLTDD EYTKLYAIPQ NFVKEIESLK NENNVRLIPK SKVTNFWQNI MLTYNSVTSL SEPVTDAMNT TMAEYSLYIE RATEAAKLIR EITNTIKDIF NPVWDVREQT GIFGLKGAEY NALEGNMIQS LLSFAGLFRQ LMSRTATVDE IGALYPKNDK NEDVIHTAID DYVNSLADLK ANEQVKLNGL LSLVYAYYAS TLGFAKKDVF NNAQASFTDY TNFLNQEIQY WTPRETSSFN ISNQALQTFK NKPSADYNGV YLFDNKGLET NLFNPTFFFD VVSLMTADPT KTMSRQDYNK VITASESSIQ KINQAITAWE LAIAECGTKK AKLEPSSLNY FNAMVEAKKT FVETSPIQMV YSSLMLDKYL PNQQYILETL GSQMTFSNKA ARYLNDIIAY AVSFQTADVY YSLGMYLRQM NQQEFPEVIS RANDTVKKEI DRSRADLFHC KKAIEKIKEL VTSVNADTEL TSSQRAELLE TLASYAFEFE NLYHNLSNVY VMVSKVQISG VSKPDEVDEA FTAKIGSKEF DTWIQQLTTF ESAVIEGGRN GVMPGGEQQV LQSLESKQQD YTSFNQNQQL ALQMESAAIQ QEWTMVAAAL ALMNQIFAKL IRRFK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcaa tacaacctac atccatttct ttaactaaga atataacggc agctttagcc ggagagcagg tcgatgctgc tgcagtgtat atgccgcagg ctgttttttt ctttcagcaa ctggatgaaa aaagcaaggg gctgaaacag gctttaggat tgctcgaaga ggttgatcta gaaaaattta taccgtcttt agaaaaatca cctacaccta tcactacggg aacaacgagt aaaatttccg ctgatgggat tgagattgtt ggagagcttt cttcagaaac aattttggca gatcctaata aagctgcagc tcaggttttt ggagaggggc ttgcagatag ttttgatgat tggctcagat tatctgaaaa tggggggatt caagatccta cagcaataga agaagagatt gttactaagt atcaaacaga actcaatact ctgcgcaata aactcaagca acaatcttta acagacgatg agtatacgaa gctttatgct attcctcaaa actttgttaa agagatagaa agcttaaaga atgaaaataa tgtgaggtta attcccaaaa gtaaagtcac taacttttgg cagaatatca tgctcactta caactcggta acctcgttat cagaacctgt taccgatgcg atgaatacga ctatggcgga gtactctctt tatattgaga gagctacaga ggctgccaag ttgatacggg agataaccaa cacgatcaaa gacattttca atccagtttg ggatgtgcgt gaacaaacag gaatttttgg gttaaaagga gctgagtata acgctttaga aggcaatatg attcaaagct tgcttagctt tgcgggtcta ttccggcagt taatgagtcg tactgcaaca gttgatgaga taggcgcact ttatcctaaa aatgataaaa acgaagacgt cattcatact gctattgatg attatgtgaa ttctttagct gatttgaaag ccaatgaaca ggtcaaactc aacggtctgt tgagtttagt atatgcttat tatgctagta ctttaggttt tgctaagaag gatgtattca ataatgcaca agcttctttt acagattata ctaattttct aaaccaagag atccaatatt ggacgcctag agagacttca agttttaata tctccaatca agcattgcaa acctttaaaa ataagccttc ggctgattat aacggcgtat atctttttga taataaagga ttagagacta atctctttaa tcctacgttc ttctttgatg ttgtgagtct catgacagct gatcctacga agactatgtc tcgacaggat tacaataagg tgattacagc ctcggaatcc agtattcaga agattaatca ggctattacc gcttgggaac tagctattgc agaatgtggg actaaaaaag cgaagctcga accatccagt ttaaattatt ttaatgctat ggtcgaagcg aagaagacct tcgtagagac ctctccaata cagatggtct attcatcttt gatgttggat aagtatcttc cgaatcagca gtacatatta gagacattag gaagtcagat gactttctct aacaaggctg ctcggtattt aaatgatatc attgcgtatg cagttagctt ccaaacagct gacgtctatt attctttagg gatgtatctt cgacaaatga accagcagga atttcctgag gtgatttctc gtgctaacga tactgtgaaa aaagagatag atcggagtcg tgcggatctc tttcactgta aaaaagctat cgaaaagatt aaagaattag tgacttctgt aaatgcggat actgaattga cctcatctca gcgtgcagag ttattagaga cgttagctag ttatgctttt gaatttgaga atctctatca caacctctct aatgtttacg tcatggtttc taaggtacag atttctggcg taagcaagcc tgatgaagtg gatgaggctt ttactgctaa gattggatcg aaggaattcg atacttggat tcagcagctt acaacatttg aaagtgctgt gattgaaggt gggcgtaatg gtgtgatgcc tgggggagag cagcaggttt tacagagttt agagagcaag cagcaagatt acacgtcgtt caaccagaat cagcaattag ctctacaaat ggagtccgca gcgattcaac aagagtggac tatggtagca gcagccttag cattaatgaa tcagattttt gctaagttga tccgtagatt taaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for ChtrB.20243.a.B1.PW38629
PURIFICATION DATe: 6/4/2019
CONCENTRATION: 10mg/ml
OBSERVED MW: 87kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS IQPTSISLTK NITAALAGEQ VDAAAVYMPQ AVFFFQQLDE KSKGLKQALG LLEEVDLEKF IPSLEKSPTP ITTGTTSKIS ADGIEIVGEL SSETILADPN KAAAQVFGEG LADSFDDWLR LSENGGIQDP TAIEEEIVTK YQTELNTLRN KLKQQSLTDD EYTKLYAIPQ NFVKEIESLK NENNVRLIPK SKVTNFWQNI MLTYNSVTSL SEPVTDAMNT TMAEYSLYIE RATEAAKLIR EITNTIKDIF NPVWDVREQT GIFGLKGAEY NALEGNMIQS LLSFAGLFRQ LMSRTATVDE IGALYPKNDK NEDVIHTAID DYVNSLADLK ANEQVKLNGL LSLVYAYYAS TLGFAKKDVF NNAQASFTDY TNFLNQEIQY WTPRETSSFN ISNQALQTFK NKPSADYNGV YLFDNKGLET NLFNPTFFFD VVSLMTADPT KTMSRQDYNK VITASESSIQ KINQAITAWE LAIAECGTKK AKLEPSSLNY FNAMVEAKKT FVETSPIQMV YSSLMLDKYL PNQQYILETL GSQMTFSNKA ARYLNDIIAY AVSFQTADVY YSLGMYLRQM NQQEFPEVIS RANDTVKKEI DRSRADLFHC KKAIEKIKEL VTSVNADTEL TSSQRAELLE TLASYAFEFE NLYHNLSNVY VMVSKVQISG VSKPDEVDEA FTAKIGSKEF DTWIQQLTTF ESAVIEGGRN GVMPGGEQQV LQSLESKQQD YTSFNQNQQL ALQMESAAIQ QEWTMVAAAL ALMNQIFAKL IRRFK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTCAA TACAACCTAC ATCCATTTCT TTAACTAAGA ATATAACGGC AGCTTTAGCC GGAGAGCAGG TCGATGCTGC TGCAGTGTAT ATGCCGCAGG CTGTTTTTTT CTTTCAGCAA CTGGATGAAA AAAGCAAGGG GCTGAAACAG GCTTTAGGAT TGCTCGAAGA GGTTGATCTA GAAAAATTTA TACCGTCTTT AGAAAAATCA CCTACACCTA TCACTACGGG AACAACGAGT AAAATTTCCG CTGATGGGAT TGAGATTGTT GGAGAGCTTT CTTCAGAAAC AATTTTGGCA GATCCTAATA AAGCTGCAGC TCAGGTTTTT GGAGAGGGGC TTGCAGATAG TTTTGATGAT TGGCTCAGAT TATCTGAAAA TGGGGGGATT CAAGATCCTA CAGCAATAGA AGAAGAGATT GTTACTAAGT ATCAAACAGA ACTCAATACT CTGCGCAATA AACTCAAGCA ACAATCTTTA ACAGACGATG AGTATACGAA GCTTTATGCT ATTCCTCAAA ACTTTGTTAA AGAGATAGAA AGCTTAAAGA ATGAAAATAA TGTGAGGTTA ATTCCCAAAA GTAAAGTCAC TAACTTTTGG CAGAATATCA TGCTCACTTA CAACTCGGTA ACCTCGTTAT CAGAACCTGT TACCGATGCG ATGAATACGA CTATGGCGGA GTACTCTCTT TATATTGAGA GAGCTACAGA GGCTGCCAAG TTGATACGGG AGATAACCAA CACGATCAAA GACATTTTCA ATCCAGTTTG GGATGTGCGT GAACAAACAG GAATTTTTGG GTTAAAAGGA GCTGAGTATA ACGCTTTAGA AGGCAATATG ATTCAAAGCT TGCTTAGCTT TGCGGGTCTA TTCCGGCAGT TAATGAGTCG TACTGCAACA GTTGATGAGA TAGGCGCACT TTATCCTAAA AATGATAAAA ACGAAGACGT CATTCATACT GCTATTGATG ATTATGTGAA TTCTTTAGCT GATTTGAAAG CCAATGAACA GGTCAAACTC AACGGTCTGT TGAGTTTAGT ATATGCTTAT TATGCTAGTA CTTTAGGTTT TGCTAAGAAG GATGTATTCA ATAATGCACA AGCTTCTTTT ACAGATTATA CTAATTTTCT AAACCAAGAG ATCCAATATT GGACGCCTAG AGAGACTTCA AGTTTTAATA TCTCCAATCA AGCATTGCAA ACCTTTAAAA ATAAGCCTTC GGCTGATTAT AACGGCGTAT ATCTTTTTGA TAATAAAGGA TTAGAGACTA ATCTCTTTAA TCCTACGTTC TTCTTTGATG TTGTGAGTCT CATGACAGCT GATCCTACGA AGACTATGTC TCGACAGGAT TACAATAAGG TGATTACAGC CTCGGAATCC AGTATTCAGA AGATTAATCA GGCTATTACC GCTTGGGAAC TAGCTATTGC AGAATGTGGG ACTAAAAAAG CGAAGCTCGA ACCATCCAGT TTAAATTATT TTAATGCTAT GGTCGAAGCG AAGAAGACCT TCGTAGAGAC CTCTCCAATA CAGATGGTCT ATTCATCTTT GATGTTGGAT AAGTATCTTC CGAATCAGCA GTACATATTA GAGACATTAG GAAGTCAGAT GACTTTCTCT AACAAGGCTG CTCGGTATTT AAATGATATC ATTGCGTATG CAGTTAGCTT CCAAACAGCT GACGTCTATT ATTCTTTAGG GATGTATCTT CGACAAATGA ACCAGCAGGA ATTTCCTGAG GTGATTTCTC GTGCTAACGA TACTGTGAAA AAAGAGATAG ATCGGAGTCG TGCGGATCTC TTTCACTGTA AAAAAGCTAT CGAAAAGATT AAAGAATTAG TGACTTCTGT AAATGCGGAT ACTGAATTGA CCTCATCTCA GCGTGCAGAG TTATTAGAGA CGTTAGCTAG TTATGCTTTT GAATTTGAGA ATCTCTATCA CAACCTCTCT AATGTTTACG TCATGGTTTC TAAGGTACAG ATTTCTGGCG TAAGCAAGCC TGATGAAGTG GATGAGGCTT TTACTGCTAA GATTGGATCG AAGGAATTCG ATACTTGGAT TCAGCAGCTT ACAACATTTG AAAGTGCTGT GATTGAAGGT GGGCGTAATG GTGTGATGCC TGGGGGAGAG CAGCAGGTTT TACAGAGTTT AGAGAGCAAG CAGCAAGATT ACACGTCGTT CAACCAGAAT CAGCAATTAG CTCTACAAAT GGAGTCCGCA GCGATTCAAC AAGAGTGGAC TATGGTAGCA GCAGCCTTAG CATTAATGAA TCAGATTTTT GCTAAGTTGA TCCGTAGATT TAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT