ChtrB.19228.a

Pseudouridine synthase (EC 5.4.99.-)

CENTER ID:	ChtrB.19228.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.19228.a.B1.GE42426	full length		1	334

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82031

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.724
UniProt:	O84665

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLEIPAPTAV FSFVIDEQLT GRLDKGLVSY NGAYSRAFYQ QQIELGRVRV NGRVYTRVSH PLSLGDVVEV ELIEEEEPSS LIPEDIPLDK VYEDDMMLVI NKPRDMVVHP APGHTQGTVV HALLHEIGER LKQEFPEEPW RPGIVHRLDK DTSGLLITVK TRRAKALYSE LFATKQVKKL YVAICINAPS QERIHTRIAR HPLKRKEMTV LSTHAEGGKE AITHCQVLAT NGRLSVVALY PETGRTHQLR VHMKHLGTPI LGDPVYGIPS INFRYGLDKQ QLHAYSLVFA HPESAERVKL VTKLPDDMTS LIEKEFREGV SILDGSCDWF KITR

NT Sequence

atgctggaaa tccctgctcc aacggctgtt ttttcatttg taattgatga acaattaaca ggacgccttg ataaggggct ggtctcgtat aatggagcgt attcacgagc tttttatcag caacaaattg agttagggcg tgtccgtgtt aatgggcggg tgtatacgcg tgtttcccat ccgttatctc taggggatgt ggtagaggtg gaattgatag aagaggaaga accctcttct ttaatcccgg aagatattcc tctggataag gtatatgaag acgatatgat gttggtcatt aataaaccaa gagatatggt tgtccatcct gctcctgggc atacgcaagg tacggtggtg catgctttgc tgcatgaaat tggggagcgt ctgaaacaag agttccccga ggagccatgg cgcccaggga ttgtacatcg cttagataaa gatacttctg gtttgttgat tacagtaaaa actcggcgag ctaaggctct ctatagtgag ctctttgcta ccaaacaggt aaaaaagctt tacgtagcca tttgcattaa tgctccgtct caggaacgta ttcatactcg gatagcgcgt catcctctaa aaaggaagga aatgaccgtg ctttctacac atgccgaagg cggtaaggaa gcaattaccc attgccaagt tctagctacg aatggacgat taagtgtggt tgctctatac ccagaaacag gcagaaccca ccagcttcgt gtacatatga agcacctggg cacaccgatt ctcggagatc ctgtttacgg gatcccctct ataaattttc gttatggtct tgacaaacaa caattgcatg cctatagctt ggtttttgct catccggaga gtgcggagcg agtgaagcta gtgacaaagc ttccagacga tatgacttcc ttaatagaaa aggaatttag agaaggtgtc tctatactgg atggttcgtg tgattggttt aaaatcacta ggtag

Details for ChtrB.19228.a.B1.GE42426

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHML EIPAPTAVFS FVIDEQLTGR LDKGLVSYNG AYSRAFYQQQ IELGRVRVNG RVYTRVSHPL SLGDVVEVEL IEEEEPSSLI PEDIPLDKVY EDDMILVINK PRDMVVHPAP GHTQGTVVHA LLHEIGERLK QEFPEEPWRP GIVHRLDKDT SGLLITVKTR RAKALYSELF ATKQVKKLYV AICINAPSQE LIHTRITRHP LKRKEMTVLS THAEGGKEAI THCHVLATNG RLSVVALYPE TGRTHQLRVH MKHLGTPILG DPVYGIPSIN FRYGLDKQQL HAYSLVFAHP ESAERVKLVT KLPDDMTSLI EKEFREGVSI LDGS

Validated NT Sequence

acgaaccatc cagtatagag acaccttctc taaattcctt ttctattaag gaagtcatat cgtctggaag ctttgtcact agcttcactc gctccgcact ctccggatga gcaaaaacca agctataggc atgcaattgt tgtttgtcaa gaccataacg aaaatttata gaggggatcc cgtaaacggg atctccgaga atcggtgtgc ccaggtgctt catatgtaca cgaagctggt gggttctgcc tgtttctggg tatagagcaa ccacacttaa tcgtccattc gtagctagaa catgacaatg ggtaatggct tccttaccgc cttcagcatg tgtagaaagc acagtcattt ctttccgttt tagaggatga cgcgttatcc gagtatgaat aagttcctga gacggagcat taatgcaaat ggctacgtaa agctttttta cctgtttggt agcaaaaagc tcactataaa gagccttagc tcgccgagtt tttaccgtaa tcaataagcc ggaagtatct ttatctaagc gatgtacaat ccctgggcgc catggctcct cggggaactc ttgtttcaga cgctctccga tttcatgtaa taaagcatgc accaccgtac cttgcgtatg cccaggagca ggatggacaa ccatatctct tggtttatta atgaccaaaa tcatatcgtc ttcatatacc ttatccagag gaatatcttc cgggattaaa gaagagggtt cttcctcttc tatcaattcc acttctacca catcccctag ggataacgga tgggaaacac gcgtatacac ccgcccatta acacggacac gccctaactc aatttgttgc tgataaaaag ctcgtgaata cgctccatta tatgagacca gccccttatc aaggcgtcct gttaattgtt catcaattac aaatgaaaaa acagccgttg gagcagggat ttccagcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHML EIPAPTAVFS FVIDEQLTGR LDKGLVSYNG AYSRAFYQQQ IELGRVRVNG RVYTRVSHPL SLGDVVEVEL IEEEEPSSLI PEDIPLDKVY EDDMMLVINK PRDMVVHPAP GHTQGTVVHA LLHEIGERLK QEFPEEPWRP GIVHRLDKDT SGLLITVKTR RAKALYSELF ATKQVKKLYV AICINAPSQE RIHTRIARHP LKRKEMTVLS THAEGGKEAI THCQVLATNG RLSVVALYPE TGRTHQLRVH MKHLGTPILG DPVYGIPSIN FRYGLDKQQL HAYSLVFAHP ESAERVKLVT KLPDDMTSLI EKEFREGVSI LDGSCDWFKI TR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgg aaatccctgc tccaacggct gttttttcat ttgtaattga tgaacaatta acaggacgcc ttgataaggg gctggtctcg tataatggag cgtattcacg agctttttat cagcaacaaa ttgagttagg gcgtgtccgt gttaatgggc gggtgtatac gcgtgtttcc catccgttat ctctagggga tgtggtagag gtggaattga tagaagagga agaaccctct tctttaatcc cggaagatat tcctctggat aaggtatatg aagacgatat gatgttggtc attaataaac caagagatat ggttgtccat cctgctcctg ggcatacgca aggtacggtg gtgcatgctt tgctgcatga aattggggag cgtctgaaac aagagttccc cgaggagcca tggcgcccag ggattgtaca tcgcttagat aaagatactt ctggtttgtt gattacagta aaaactcggc gagctaaggc tctctatagt gagctctttg ctaccaaaca ggtaaaaaag ctttacgtag ccatttgcat taatgctccg tctcaggaac gtattcatac tcggatagcg cgtcatcctc taaaaaggaa ggaaatgacc gtgctttcta cacatgccga aggcggtaag gaagcaatta cccattgcca agttctagct acgaatggac gattaagtgt ggttgctcta tacccagaaa caggcagaac ccaccagctt cgtgtacata tgaagcacct gggcacaccg attctcggag atcctgttta cgggatcccc tctataaatt ttcgttatgg tcttgacaaa caacaattgc atgcctatag cttggttttt gctcatccgg agagtgcgga gcgagtgaag ctagtgacaa agcttccaga cgatatgact tccttaatag aaaaggaatt tagagaaggt gtctctatac tggatggttc gtgtgattgg tttaaaatca ctaggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat