ChtrB.18890.a

Phenylalanine--tRNA ligase beta subunit (EC 6.1.1.20) (Phenylalanyl-tRNA synthetase beta subunit) (PheRS)

CENTER ID:	ChtrB.18890.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.18890.a.B1.GE42424	full length		1	790

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82010

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.514
UniProt:	O84481

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLVPLSLLQK FFSSPLSIEE ILQACDRIGI EAECSNVFPD SLNTVVTGKI LSASPHPDAE RLTVAIVFDG KGERQIICGA PNCRAGIIVP IALPGAKLRN ASGEITTIKK AKIRGLESQG MCCGADELGF PHLQKAERGI FEFPADTPLG ESACMLLAGA PLECSLTPNL GHCASLLGLA REISFLSPVS LNIPEEFSFA SLPQETSICD MHDAGACPVF YSVKISGLSC RRSPEYLQAA LTALGQKPLN AIVDITNYVM LSLGQPLHAY DSQAVEQKSL HAATLQSAQP LTLLNQETYT LPAGSLVVAD QHNILGLAGV MGSAASSCSE NTTEIILEAA YFQPQAVRKY QRTIQLHTEA AYRFTRGVDP QGVLPVLHAA IHMIQSLFPD AQISPIQKIG DDSFSPLSLS VRPKTIKRLL DIELSTAEIV AKLSSLGFQT AVEEQAVRVE VPSYRHDIQE ETDLVEEICR TTPFVQKTQK ILPTYTPIYS LKRELTAFLA NGGLQQFFTY SLLDTEVSSL SLQESSLIPV QNSSWKLRDS LLPGMLKSAA TNLHRQAPYV YAFEIGNVYS KEQNRYQEEE RVAILLSRQV MDDSWQGKTP LSFYTIKGWV EKLLCQSGAS IEDFSLQPSQ HPNFHPYQQA ALYQKKHLLG IFGTLHPQLC RKAQIKHDVV FAELSLNVLL SLKKKSGPHY VPYPIYPASS RDITITIDRD LPADLVRREL LSFESKWLES VHIVSVYQGR DSASQSKNVS LRMVFRDHER TLSGQEIEEE YERLTALLDK KLANIGQGNS

NT Sequence

atgctcgttc ctttatccct attacaaaaa ttcttctctt ctcctctctc tattgaggaa attttacaag cctgtgatcg tattggaatc gaagcggaat gctcaaatgt ttttccggat tcccttaata ctgttgtaac agggaaaatt ttaagtgcct ctcctcatcc tgatgcagaa agacttactg ttgccatcgt ttttgacggg aagggtgagc gccaaatcat ctgtggagct cccaactgcc gagcaggcat cattgttcct atagccttac caggagctaa gttacgtaat gcttcgggag aaatcactac aatcaaaaaa gctaaaattc gtgggctcga atcacaagga atgtgttgtg gagcagacga attagggttc cctcatttac aaaaagcaga gcgtggtatt tttgagttcc ctgcagatac tcctcttgga gaaagtgcct gcatgctact tgctggagct cccctagaat gctcgttaac ccctaattta ggccattgcg catccttact aggcctagca agagaaattt cctttctatc ccctgtatct cttaatatcc cggaagaatt ttcttttgcc tcgcttcctc aagaaacttc tatttgtgat atgcacgatg ctggagcttg ccccgtattc tattccgtca aaatctcggg tctatcttgt cgacgatccc cagaatatct gcaagcagcc ttaacagctc ttggacaaaa acctcttaac gccatcgttg atattacgaa ttatgtgatg ctgtcattag gtcagcccct acatgcttac gatagccaag cggtggagca aaagtctctc catgcagcca ctctacagtc agctcaacca cttactcttc taaatcaaga aacctatacc ctccccgcag gatctttagt cgttgctgat cagcataata tcttaggatt agctggggtc atgggaagtg ctgcatcttc ttgctcagaa aatacaacag aaatcattct ggaagccgct tacttccagc ctcaggctgt aagaaaatat caacgcacga tacagctgca cacagaagct gcctatagat ttacccgagg agtggatcct caaggcgtat tacccgtgtt acatgctgct atccatatga ttcaatctct atttccggat gctcaaatct ctcctattca aaaaattgga gatgatagct tttctccttt gtctttaagc gttcgcccta aaacgattaa aagacttctg gatattgagc tctcgaccgc agaaatagtt gccaaactct cttctttagg atttcaaaca gcagtagaag agcaggccgt tcgagtcgag gtcccctctt accgtcatga cattcaggaa gagaccgacc ttgttgaaga gatctgtcgc accacaccat tcgttcaaaa aacacaaaaa atactcccga cctatactcc gatctattct ttgaaacgtg aactcacagc tttcttagca aatggtgggc tacaacaatt ctttacctac tccttgctgg atacagaagt atcttcctta tctttgcaag aaagctcttt gattcctgta cagaattctt cctggaaatt acgcgactcc cttcttcctg ggatgctaaa aagtgcggct actaacctcc ataggcaagc cccctatgtt tatgcttttg aaattggaaa tgtgtactcg aaggagcaga accgttacca agaggaagag cgtgttgcta ttttactctc tcgccaagta atggatgatt cctggcaagg gaaaacacct ctctcttttt atacaatcaa aggatgggtg gaaaaactat tatgccagtc aggagcttct atagaagact tttctcttca acccagccag catccgaact ttcaccctta tcagcaagct gccttgtatc agaaaaaaca tctattaggc atctttggga cgttgcaccc acaactatgt agaaaagctc agatcaaaca cgatgtagtc tttgctgagc tttctttaaa tgtgcttttg tctttaaaaa agaaatccgg acctcattac gtaccctatc caatctatcc ggcctcttcc agagacatta caattacaat agatagagat cttccagcag atctcgtacg cagagaactt ttaagtttcg aatctaaatg gcttgaaagt gttcatattg tcagtgtata tcaagggaga gactctgcat ctcaaagtaa aaacgtttct cttcggatgg tcttccgtga tcacgaacga accttatccg ggcaggaaat agaagaagaa tacgaacgtt taactgcatt acttgataag aaattagcca acataggaca aggcaactca tga

Details for ChtrB.18890.a.B1.GE42424

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	88kDa
OBSERVED MW:	88kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML VPLSLLQKFF SSPLSIEEIL QACDRIGIEA ECSNVFPDSL NTVVTGKILS ASPHPDAERL TVAIVFDGKG ERQIICGAPN CRAGIIVPIA LPGAKLRNAS GEITTIKKAK IRGLESQGMC CGADELGFPH LQKAERGIFE FPADTPLGES ACMLLAGAPL ECSLTPNLGH CASLLGLARE ISFLSPVSLN IPEEFSFASL PQETSICDMH DAGACPVFYS VKISGLSCRR SPEYLQAALT ALGQKPLNAI VDITNYVMLS LGQPLHAYDS QAVEQKSLHA ATLQSAQPLT LLNQETYTLP AGSLVVADQH NILGLAGVMG SAASSCSENT TEIILEAAYF QPQAVRKYQR TIQLHTEAAY RFTRGVDPQG VLPVLHAAIH MIQSLFPDAQ ISPIQKIGDD SFSPLSLSVR PKTIKRLLDI ELSTAEIVAK LSSLGFQTAV EEQAVRVEVP SYRHDIQEET DLVEEICRTT PFVQKTQKIL PTYTPIYSLK RELTAFLANG GLQQFFTYSL LDTEVSSLSL QESSLIPVQN SSWKLRDSLL PGMLKSAATN LHRQAPYVYA FEIGNVYSKE QNRYQEEERV AILLSRQVMD DSWQGKTPLS FYTIKGWVEK LLCQSGASIE DFSLQPSQHP NFHPYQQAAL YQKKHLLGIF GTLHPQLCRK AQIKHDVVFA ELSLNVLLSL KKKSGPHYVP YPIYPASSRD ITITIDRDLP ADLVRRELLS FESKWLESVH IVSVYQGRDS ASQSKNVSLR MVFRDHERTL SGQEIEEEYE RLTALLDKKL ANIGQGNS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg ttcctttatc cctattacaa aaattcttct cttctcctct ctctattgag gaaattttac aagcctgtga tcgtattgga atcgaagcgg aatgctcaaa tgtttttccg gattccctta atactgttgt aacagggaaa attttaagtg cctctcctca tcctgatgca gaaagactta ctgttgccat cgtttttgac gggaagggtg agcgccaaat catctgtgga gctcccaact gccgagcagg catcattgtt cctatagcct taccaggagc taagttacgt aatgcttcgg gagaaatcac tacaatcaaa aaagctaaaa ttcgtgggct cgaatcacaa ggaatgtgtt gtggagcaga cgaattaggg ttccctcatt tacaaaaagc agagcgtggt atttttgagt tccctgcaga tactcctctt ggagaaagtg cctgcatgct acttgctgga gctcccctag aatgctcgtt aacccctaat ttaggccatt gcgcatcctt actaggccta gcaagagaaa tttcctttct atcccctgta tctcttaata tcccggaaga attttctttt gcctcgcttc ctcaagaaac ttctatttgt gatatgcacg atgctggagc ttgccccgta ttctattccg tcaaaatctc gggtctatct tgtcgacgat ccccagaata tctgcaagca gccttaacag ctcttggaca aaaacctctt aacgccatcg ttgatattac gaattatgtg atgctgtcat taggtcagcc cctacatgct tacgatagcc aagcggtgga gcaaaagtct ctccatgcag ccactctaca gtcagctcaa ccacttactc ttctaaatca agaaacctat accctccccg caggatcttt agtcgttgct gatcagcata atatcttagg attagctggg gtcatgggaa gtgctgcatc ttcttgctca gaaaatacaa cagaaatcat tctggaagcc gcttacttcc agcctcaggc tgtaagaaaa tatcaacgca cgatacagct gcacacagaa gctgcctata gatttacccg aggagtggat cctcaaggcg tattacccgt gttacatgct gctatccata tgattcaatc tctatttccg gatgctcaaa tctctcctat tcaaaaaatt ggagatgata gcttttctcc tttgtcttta agcgttcgcc ctaaaacgat taaaagactt ctggatattg agctctcgac cgcagaaata gttgccaaac tctcttcttt aggatttcaa acagcagtag aagagcaggc cgttcgagtc gaggtcccct cttaccgtca tgacattcag gaagagaccg accttgttga agagatctgt cgcaccacac cattcgttca aaaaacacaa aaaatactcc cgacctatac tccgatctat tctttgaaac gtgaactcac agctttctta gcaaatggtg ggctacaaca attctttacc tactccttgc tggatacaga agtatcttcc ttatctttgc aagaaagctc tttgattcct gtacagaatt cttcctggaa attacgcgac tcccttcttc ctgggatgct aaaaagtgcg gctactaacc tccataggca agccccctat gtttatgctt ttgaaattgg aaatgtgtac tcgaaggagc agaaccgtta ccaagaggaa gagcgtgttg ctattttact ctctcgccaa gtaatggatg attcctggca agggaaaaca cctctctctt tttatacaat caaaggatgg gtggaaaaac tattatgcca gtcaggagct tctatagaag acttttctct tcaacccagc cagcatccga actttcaccc ttatcagcaa gctgccttgt atcagaaaaa acatctatta ggcatctttg ggacgttgca cccacaacta tgtagaaaag ctcagatcaa acacgatgta gtctttgctg agctttcttt aaatgtgctt ttgtctttaa aaaagaaatc cggacctcat tacgtaccct atccaatcta tccggcctct tccagagaca ttacaattac aatagataga gatcttccag cagatctcgt acgcagagaa cttttaagtt tcgaatctaa atggcttgaa agtgttcata ttgtcagtgt atatcaaggg agagactctg catctcaaag taaaaacgtt tctcttcgga tggtcttccg tgatcacgaa cgaaccttat ccgggcagga aatagaagaa gaatacgaac gtttaactgc attacttgat aagaaattag ccaacatagg acaaggcaac tcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat