SSGCID: Target Page

ChtrB.18857.a

Translation initiation factor IF-3

CENTER ID:	ChtrB.18857.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.18857.a.B1.GE42423	full length		1	175

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82023

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272561.5.peg.920
UniProt:	O84840

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALNLRINRQ IRAPRVRVIG SAGEQLGILS IKEALDLAKE ANLDLVEVAS NSEPPVCKIM DYGKYRYDVT KKEKDSKKAQ HQVRIKEVKL KPNIDDNDFL TKAKQARAFI EKGNKVKVSC MFRGRELAYP EHGYKVIQRM CQGLEDIGFV ESEPKLNGRS LICVIAPGTL KTKKK

NT Sequence

gtggctttaa acctaagaat aaataggcag atacgagctc ctagggtacg tgtaataggt tccgcaggag agcagctagg catattgagt ataaaagagg ccctagattt agccaaggaa gctaatttag accttgttga ggttgcttca aactcagagc ctcccgtgtg caaaatcatg gactatggga agtatcgtta cgacgtaact aaaaaagaaa aagatagtaa gaaagcacag caccaagtac gtatcaaaga ggttaagctt aagcctaata tcgatgataa cgactttctt acgaaagcaa agcaagctag agcctttatt gagaaaggaa ataaagtaaa ggtttcttgt atgtttcggg ggcgagagtt ggcttatccc gaacacgggt ataaggttat tcaaagaatg tgtcagggct tagaggacat aggttttgtt gagtcagagc ctaaactgaa tggccgttct ttgatctgtg ttattgctcc gggaacacta aaaactaaga aaaaatag

Details for ChtrB.18857.a.B1.GE42423

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	21kDa
OBSERVED MW:	21kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV ALNLRINRQI RAPRVRVIGS AGEQLGILSI KEALDLAKEA DLDLVEVASN SEPPVCKIMD YGKYRYDITE KEKDSKKAQH QVRIKEVKLK PNIDDNDFLT KAKQARAFIE KGNKVKVSCM FRGRELAYPE HGHKVVQRMC QGLEDIGFVE SEPKLNGRSL ICVIAPGTLK TKKK

Validated NT Sequence

atcttactca cttatttttt cttagttttt agtgttcccg gagcaataac acagatcaaa gaacggccat tcagtttagg ctctgactca acaaaaccta tgtcctctaa gccctgacac attctttgaa caaccttatg cccgtgttcg ggataagcca actctcgccc ccgaaacata caagaaacct ttactttatt tcctttctca ataaaggctc tagcttgctt tgctttcgta agaaagtcgt tatcatcgat attaggctta agcttaacct ctttgatacg cacttggtgc tgtgctttct tactatcttt ttctttttca gttatgtcgt aacgatactt cccgtagtcc atgattttgc acacaggagg ctctgagttt gaagcaacct caacaaggtc taaatcagct tccttggcta aatctagggc ctcttttata ctcaatatgc ctagctgctc tcctgcggaa cctattacac gtaccctagg agctcgtatc tgcctattta ttcttaggtt taaagccacc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMA LNLRINRQIR APRVRVIGSA GEQLGILSIK EALDLAKEAN LDLVEVASNS EPPVCKIMDY GKYRYDVTKK EKDSKKAQHQ VRIKEVKLKP NIDDNDFLTK AKQARAFIEK GNKVKVSCMF RGRELAYPEH GYKVIQRMCQ GLEDIGFVES EPKLNGRSLI CVIAPGTLKT KKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggctt taaacctaag aataaatagg cagatacgag ctcctagggt acgtgtaata ggttccgcag gagagcagct aggcatattg agtataaaag aggccctaga tttagccaag gaagctaatt tagaccttgt tgaggttgct tcaaactcag agcctcccgt gtgcaaaatc atggactatg ggaagtatcg ttacgacgta actaaaaaag aaaaagatag taagaaagca cagcaccaag tacgtatcaa agaggttaag cttaagccta atatcgatga taacgacttt cttacgaaag caaagcaagc tagagccttt attgagaaag gaaataaagt aaaggtttct tgtatgtttc gggggcgaga gttggcttat cccgaacacg ggtataaggt tattcaaaga atgtgtcagg gcttagagga cataggtttt gttgagtcag agcctaaact gaatggccgt tctttgatct gtgttattgc tccgggaaca ctaaaaacta agaaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat