ChtrB.18178.a

Lon protease (EC 3.4.21.53) (ATP-dependent protease La)

CENTER ID:	ChtrB.18178.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.18178.a.B1.GE42421	full length		1	819
ChtrB.18178.a.B4.GE43133	XtalPred domain #1		43	819

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82014

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.372
UniProt:	O84348

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNSTNNTDSQ NLDPNASEVE KLLDESAEAE EKVDDHTPPS ELFILPLNKR PFFPGMAAPL LIEAGPHYEV LTLLAKSSQK HIGLVLTKKE DANTLKVGFN QLHRVGVSAR ILRIMPIEGG SAQVLLSIED RIRIVKPIQD KYLKAKVSYH KENKELTEEL KAYSISIVSI IKDLLKLNPL FKEELQIFLG HSDFTEPGKL ADFSVALTTA TREELQEILE TTDMHDRIDK ALVLLKKELD LSRLQSSINQ KIEATITKSQ KEFFLKEQLK TIKKELGLEK DDHAVDLEKF MERFNKRDVP QYAMDVIQDE MDKLQTLETS SAEYAVCRNY LDWLTIVPWG IQTKEYHDLK KAESILNKDH YGLEDIKQRI LELISVGKLA NGMKGSIICL VGPPGVGKTS IGRSIAKVLH RKFFRFSVGG MRDEAEIKGH RRTYIGAMPG KLVQALKQSQ IMNPVIMIDE VDKIGSSYHG DPASALLEVL DPEQNKDFLD HYLDVRVDLS NVLFILTANV LDSIPDPLLD RMEVLRLSGY ILEEKLQIAT KYLVPRARKE MGLSAQNVTF QPEALKHMIN NYAREAGVRT LNENIKKVLR KVALKIVQNQ EKNLSKKSRF TITPKNLQDY LGKPVFSSDR FYEKTPVGVA TGLAWTSLGG ATLYIESVQV PSSSGKADMH LTGQAGDVMK ESSQIAWTYL HSALERYAPG QPFFEKSQVH IHIPEGATPK DGPSAGITMV TSLLSLLLDV PVLNNLGMTG ELTLTGRVLG IGGIREKLIA ARRSKLNILI FPEDNRRDYD ELPAYLKKGL KVHFVTHYDD VFKIAFPGV

NT Sequence

gtgaactcga cgaataatac agactctcaa aatctagatc caaatgcttc agaggtcgaa aaacttctcg acgaatccgc agaggcagaa gagaaagtag atgatcatac gcccccttca gaacttttta ttctccctct gaataagcgt ccttttttcc ctggtatggc agcccctctc cttatagagg caggccccca ttatgaagta cttacactat tagcaaaatc atcccagaaa cacatcggtc ttgtcttaac aaaaaaagaa gatgccaaca ctctaaaagt tggttttaat cagcttcatc gagtgggcgt atccgcgcgc attttacgca taatgcctat tgaaggcggt agcgctcaag tgttattgag tatagaagac cgtattcgca tcgttaagcc aattcaagat aagtatctaa aagccaaagt ctcctatcac aaagagaaca aagagctgac tgaagagcta aaagcttact caattagcat cgtatcgatt atcaaagatc ttctaaaact aaaccctctg ttcaaagaag agctacaaat tttccttggc cactctgatt tcactgagcc agggaaactc gcagatttct ccgttgcttt aactacagca acacgagagg aacttcagga aattttagaa accacagaca tgcatgatcg catagacaaa gcattggtct tactcaaaaa agagttagat ctaagccgtc tgcaaagtag tatcaatcaa aaaattgaag cgaccatcac aaaaagtcag aaagaatttt tcctaaaaga acaacttaaa acaataaaga aagagttagg gctagagaaa gacgatcacg ctgttgatct tgagaagttt atggaacgct tcaataaacg agacgttcct caatacgcca tggacgttat ccaagatgaa atggataaat tacaaacctt ggagacttct tcagctgaat atgcagtctg ccgcaactat ctcgattggt taactattgt accttggggt atccaaacca aggaatacca cgatttgaag aaagcagaat ctatcttaaa taaagaccat tacggtttag aagatataaa acaacgcatt cttgaattga ttagtgtagg caaactagca aacggtatga agggaagtat tatttgccta gtggggcctc caggagtagg gaaaacaagt atcggccgaa gtattgcaaa agtgttacac cgtaaattct tccggttctc tgtaggagga atgcgtgatg aagcagaaat caaaggacat agacggacct atatcggagc tatgccaggg aaacttgtgc aagctttgaa acaaagtcag attatgaatc ctgtgatcat gattgatgaa gtcgataaaa ttggctctag ttatcacgga gatccagctt cggcccttct tgaggtatta gatcccgaac aaaacaaaga ctttttagat cattatttgg atgtccgcgt agatttatct aacgtgctct tcattctaac cgcaaacgta ttagattcca tcccagaccc tctactggac cgaatggaag tattgcgttt atctggttac attctggaag aaaaattaca gatagccact aaatacctcg tacctcgcgc aaggaaagag atgggacttt ctgcacagaa tgtgactttc caaccagaag ctctcaagca catgatcaac aactatgcta gagaagctgg agttcgtaca ctgaatgaaa acattaagaa agtcttacga aaagtagctc ttaaaattgt tcaaaaccaa gagaaaaatc tttccaagaa gtcgcggttt acaatcacgc caaaaaatct acaagattat cttggcaaac cagtcttttc tagcgatcgt ttctatgaaa agactccggt aggagttgcc acaggcctag catggacttc cttaggagga gcaaccttgt acatagaaag tgttcaagtc ccttcttcat caggtaaagc ggatatgcat ctaacaggtc aagctgggga tgtgatgaaa gagtcttcac aaatagcatg gacctatctt catagtgctc tagaacgtta tgctccagga caaccattct ttgaaaaatc gcaagtccat atccatatcc cagagggagc cactccaaaa gacggtccct ctgcaggaat tactatggta acgtccttgc tttctttact tttggatgtt cctgtgctta ataacctcgg catgactgga gagctcacct taacaggcag agttttagga attggaggta tccgggaaaa acttatcgca gcaagaagat cgaaattgaa tattttaatc ttccctgaag ataaccgtcg agattacgac gagctccccg cataccttaa gaaaggtctc aaggtacact tcgtcaccca ttatgacgat gtattcaaga ttgctttccc tggggtttaa

Details for ChtrB.18178.a.B1.GE42421

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	93kDa
OBSERVED MW:	93kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN STNNTDSQNL DPNASEVEKL LDESAEAEEK VDDHTPPSEL FILPLNKRPF FPGMAAPLLI EAGPHYEVLT LLAKSSQKHI GLVLTKKEDA NTLKVGFNQL HRVGVSARIL RIMPIEGGSA QVLLSIEDRI RIVKPIQDKY LKAKVSYHKE NKELTEELKA YSISIVSIIK DLLKLNPLFK EELQIFLGHS DFTEPGKLAD FSVALTTATR EELQEILETT DMHDRIDKAL VLLKKELDLS RLQSSINQKI EATITKSQKE FFLKEQLKTI KKELGLEKDD HAVDLEKFME RFNKRDVPQY AMDVIQDEMD KLQTLETSSA EYAVCRNYLD WLTIVPWGIQ TKEYHDLKKA ESILNKDHYG LEDIKQRILE LISVGKLANG MKGSIICLVG PPGVGKTSIG RSIAKVLHRK FFRFSVGGMR DEAEIKGHRR TYIGAMPGKL VQALKQSQIM NPVIMIDEVD KIGSSYHGDP ASALLEVLDP EQNKDFLDHY LDVRVDLSNV LFILTANVLD SIPDPLLDRM EVLRLSGYIL EEKLQIATKY LVPRARKEMG LSAQNVTFQP EALKHMINNY AREAGVRTLN ENIKKVLRKV ALKIVQNQEK NLSKKSRFTI TPKNLQDYLG KPVFSSDRFY EKTPVGVATG LAWTSLGGAT LYIESVQVPS SSGKADMHLT GQAGDVMKES SQIAWTYLHS ALERYAPGQP FFEKSQVHIH IPEGATPKDG PSAGITMVTS LLSLLLDVPV LNNLGMTGEL TLTGRVLGIG GIREKLIAAR RSKLNILIFP EDNRRDYDEL PAYLKKGLKV HFVTHYDDVF KIAFPGV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaact cgacgaataa tacagactct caaaatctag atccaaatgc ttcagaggtc gaaaaacttc tcgacgaatc cgcagaggca gaagagaaag tagatgatca tacgccccct tcagaacttt ttattctccc tctgaataag cgtccttttt tccctggtat ggcagcccct ctccttatag aggcaggccc ccattatgaa gtacttacac tattagcaaa atcatcccag aaacacatcg gtcttgtctt aacaaaaaaa gaagatgcca acactctaaa agttggtttt aatcagcttc atcgagtggg cgtatccgcg cgcattttac gcataatgcc tattgaaggc ggtagcgctc aagtgttatt gagtatagaa gaccgtattc gcatcgttaa gccaattcaa gataagtatc taaaagccaa agtctcctat cacaaagaga acaaagagct gactgaagag ctaaaagctt actcaattag catcgtatcg attatcaaag atcttctaaa actaaaccct ctgttcaaag aagagctaca aattttcctt ggccactctg atttcactga gccagggaaa ctcgcagatt tctccgttgc tttaactaca gcaacacgag aggaacttca ggaaatttta gaaaccacag acatgcatga tcgcatagac aaagcattgg tcttactcaa aaaagagtta gatctaagcc gtctgcaaag tagtatcaat caaaaaattg aagcgaccat cacaaaaagt cagaaagaat ttttcctaaa agaacaactt aaaacaataa agaaagagtt agggctagag aaagacgatc acgctgttga tcttgagaag tttatggaac gcttcaataa acgagacgtt cctcaatacg ccatggacgt tatccaagat gaaatggata aattacaaac cttggagact tcttcagctg aatatgcagt ctgccgcaac tatctcgatt ggttaactat tgtaccttgg ggtatccaaa ccaaggaata ccacgatttg aagaaagcag aatctatctt aaataaagac cattacggtt tagaagatat aaaacaacgc attcttgaat tgattagtgt aggcaaacta gcaaacggta tgaagggaag tattatttgc ctagtggggc ctccaggagt agggaaaaca agtatcggcc gaagtattgc aaaagtgtta caccgtaaat tcttccggtt ctctgtagga ggaatgcgtg atgaagcaga aatcaaagga catagacgga cctatatcgg agctatgcca gggaaacttg tgcaagcttt gaaacaaagt cagattatga atcctgtgat catgattgat gaagtcgata aaattggctc tagttatcac ggagatccag cttcggccct tcttgaggta ttagatcccg aacaaaacaa agacttttta gatcattatt tggatgtccg cgtagattta tctaacgtgc tcttcattct aaccgcaaac gtattagatt ccatcccaga ccctctactg gaccgaatgg aagtattgcg tttatctggt tacattctgg aagaaaaatt acagatagcc actaaatacc tcgtacctcg cgcaaggaaa gagatgggac tttctgcaca gaatgtgact ttccaaccag aagctctcaa gcacatgatc aacaactatg ctagagaagc tggagttcgt acactgaatg aaaacattaa gaaagtctta cgaaaagtag ctcttaaaat tgttcaaaac caagagaaaa atctttccaa gaagtcgcgg tttacaatca cgccaaaaaa tctacaagat tatcttggca aaccagtctt ttctagcgat cgtttctatg aaaagactcc ggtaggagtt gccacaggcc tagcatggac ttccttagga ggagcaacct tgtacataga aagtgttcaa gtcccttctt catcaggtaa agcggatatg catctaacag gtcaagctgg ggatgtgatg aaagagtctt cacaaatagc atggacctat cttcatagtg ctctagaacg ttatgctcca ggacaaccat tctttgaaaa atcgcaagtc catatccata tcccagaggg agccactcca aaagacggtc cctctgcagg aattactatg gtaacgtcct tgctttcttt acttttggat gttcctgtgc ttaataacct cggcatgact ggagagctca ccttaacagg cagagtttta ggaattggag gtatccggga aaaacttatc gcagcaagaa gatcgaaatt gaatatttta atcttccctg aagataaccg tcgagattac gacgagctcc ccgcatacct taagaaaggt ctcaaggtac acttcgtcac ccattatgac gatgtattca agattgcttt ccctggggtt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for ChtrB.18178.a.B4.GE43133

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/24/2019
EXPECTED MW:	88kDa
OBSERVED MW:	88kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHFI LPLNKRPFFP GMAAPLLIEA GPHYEVLTLL AKSSQKHIGL VLTKKEDANT LKVGFNQLHR VGVSARILRI MPIEGGSAQV LLSIEDRIRI VKPIQDKYLK AKVSYHKENK ELTEELKAYS ISIVSIIKDL LKLNPLFKEE LQIFLGHSDF TEPGKLADFS VALTTATREE LQEILETTDM HDRIDKALVL LKKELDLSRL QSSINQKIEA TITKSQKEFF LKEQLKTIKK ELGLEKDDHA VDLEKFMERF NKRDVPQYAM DVIQDEMDKL QTLETSSAEY AVCRNYLDWL TIVPWGIQTK EYHDLKKAES ILNKDHYGLE DIKQRILELI SVGKLANGMK GSIICLVGPP GVGKTSIGRS IAKVLHRKFF RFSVGGMRDE AEIKGHRRTY IGAMPGKLVQ ALKQSQIMNP VIMIDEVDKI GSSYHGDPAS ALLEVLDPEQ NKDFLDHYLD VRVDLSNVLF ILTANVLDSI PDPLLDRMEV LRLSGYILEE KLQIATKYLV PRARKEMGLS AQNVTFQPEA LKHMINNYAR EAGVRTLNEN IKKVLRKVAL KIVQNQEKNL SKKSRFTITP KNLQDYLGKP VFSSDRFYEK TPVGVATGLA WTSLGGATLY IESVQVPSSS GKADMHLTGQ AGDVMKESSQ IAWTYLHSAL ERYAPGQPFF EKSQVHIHIP EGATPKDGPS AGITMVTSLL SLLLDVPVLN NLGMTGELTL TGRVLGIGGI REKLIAARRS KLNILIFPED NRRDYDELPA YLKKGLKVHF VTHYDDVFKI AFPGV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattttattc tccctctgaa taagcgtcct tttttccctg gtatggcagc ccctctcctt atagaggcag gcccccatta tgaagtactt acactattag caaaatcatc ccagaaacac atcggtcttg tcttaacaaa aaaagaagat gccaacactc taaaagttgg ttttaatcag cttcatcgag tgggcgtatc cgcgcgcatt ttacgcataa tgcctattga aggcggtagc gctcaagtgt tattgagtat agaagaccgt attcgcatcg ttaagccaat tcaagataag tatctaaaag ccaaagtctc ctatcacaaa gagaacaaag agctgactga agagctaaaa gcttactcaa ttagcatcgt atcgattatc aaagatcttc taaaactaaa ccctctgttc aaagaagagc tacaaatttt ccttggccac tctgatttca ctgagccagg gaaactcgca gatttctccg ttgctttaac tacagcaaca cgagaggaac ttcaggaaat tttagaaacc acagacatgc atgatcgcat agacaaagca ttggtcttac tcaaaaaaga gttagatcta agccgtctgc aaagtagtat caatcaaaaa attgaagcga ccatcacaaa aagtcagaaa gaatttttcc taaaagaaca acttaaaaca ataaagaaag agttagggct agagaaagac gatcacgctg ttgatcttga gaagtttatg gaacgcttca ataaacgaga cgttcctcaa tacgccatgg acgttatcca agatgaaatg gataaattac aaaccttgga gacttcttca gctgaatatg cagtctgccg caactatctc gattggttaa ctattgtacc ttggggtatc caaaccaagg aataccacga tttgaagaaa gcagaatcta tcttaaataa agaccattac ggtttagaag atataaaaca acgcattctt gaattgatta gtgtaggcaa actagcaaac ggtatgaagg gaagtattat ttgcctagtg gggcctccag gagtagggaa aacaagtatc ggccgaagta ttgcaaaagt gttacaccgt aaattcttcc ggttctctgt aggaggaatg cgtgatgaag cagaaatcaa aggacataga cggacctata tcggagctat gccagggaaa cttgtgcaag ctttgaaaca aagtcagatt atgaatcctg tgatcatgat tgatgaagtc gataaaattg gctctagtta tcacggagat ccagcttcgg cccttcttga ggtattagat cccgaacaaa acaaagactt tttagatcat tatttggatg tccgcgtaga tttatctaac gtgctcttca ttctaaccgc aaacgtatta gattccatcc cagaccctct actggaccga atggaagtat tgcgtttatc tggttacatt ctggaagaaa aattacagat agccactaaa tacctcgtac ctcgcgcaag gaaagagatg ggactttctg cacagaatgt gactttccaa ccagaagctc tcaagcacat gatcaacaac tatgctagag aagctggagt tcgtacactg aatgaaaaca ttaagaaagt cttacgaaaa gtagctctta aaattgttca aaaccaagag aaaaatcttt ccaagaagtc gcggtttaca atcacgccaa aaaatctaca agattatctt ggcaaaccag tcttttctag cgatcgtttc tatgaaaaga ctccggtagg agttgccaca ggcctagcat ggacttcctt aggaggagca accttgtaca tagaaagtgt tcaagtccct tcttcatcag gtaaagcgga tatgcatcta acaggtcaag ctggggatgt gatgaaagag tcttcacaaa tagcatggac ctatcttcat agtgctctag aacgttatgc tccaggacaa ccattctttg aaaaatcgca agtccatatc catatcccag agggagccac tccaaaagac ggtccctctg caggaattac tatggtaacg tccttgcttt ctttactttt ggatgttcct gtgcttaata acctcggcat gactggagag ctcaccttaa caggcagagt tttaggaatt ggaggtatcc gggaaaaact tatcgcagca agaagatcga aattgaatat tttaatcttc cctgaagata accgtcgaga ttacgacgag ctccccgcat accttaagaa aggtctcaag gtacacttcg tcacccatta tgacgatgta ttcaagattg ctttccctgg ggtttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat