ChtrB.17987.a

DNA polymerase III, beta subunit; beta sliding clamp

CENTER ID: ChtrB.17987.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.17987.a.B1.GE42420 full length 1 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.84
UniProt: O84078

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFRLHGAFS NPSFVKIFFT KASPERRLTC FHVTKHGYSF QTDLHDYRKI MKFVISRNEL GNLIKKVQNV VPQSTPIPVL THVLIESCND ELVFTATDLT VSTRCVVKAK VYESGSVTIP SRRFFQLIRE LTEANIEVAA HSGEMATITS GSSCFRLLSM GKEDFPMLPD MQNALRFTLD SERLKDMFQR TSFAVSREES RYVLTGVLLS IANGTMTVVG TDGKRLAKID TEISLDPSFS GDYIIPIKAV EEIIRMSSED VQSTIFLDQT KIAVECGNTL LVTKLLSGEF PDFSPVISTH SSVQLDLHRE ELISLLKQVA LFTNESSHSV KFSFSPGELT LTANCTKVGE GKVSMAVNYT GETLEIAFNP FFFLDILKHS RDELVQLGIS DSYNPGIITD STRSLFVIMP MRLHDD
NT Sequence
gtgaatttcc gccttcatgg ggctttttca aatccgtctt ttgtcaagat attttttact aaagctagcc ccgagagacg acttacttgc ttccatgtca caaaacatgg ttattcattc caaacagact tacatgatta caggaaaata atgaaattcg ttatctcccg caatgaatta ggaaatctaa ttaaaaaagt tcagaatgtc gttccacaaa gcacgccaat tccagtattg actcatgtac tcattgaaag ctgtaacgac gagttggttt tcacggctac agacctgacg gtcagcactc gttgtgttgt taaagcaaag gtctatgagt ctggatctgt taccatccct tctcgaagat tcttccaact cattcgtgag ttaacagagg caaatattga agtagcagct cattctggag aaatggcgac aatcacgtcg ggttcttcct gttttcgcct gttaagtatg gggaaagaag atttccctat gcttcctgat atgcaaaatg ctctgcgttt cactctggat tctgagcgat taaaagacat gttccaaaga acctcttttg ctgtatccag agaagagagt cgctatgtac tcactggcgt tttactttct attgcgaatg gaactatgac agtcgtggga actgatggga aacggttagc taagatcgat acagagattt ctttagatcc tagtttttca ggagactata tcatccctat caaagccgtc gaagaaatta ttcgcatgtc ttcggaagat gttcaatcca ctattttctt agatcaaact aaaatcgctg tcgaatgtgg aaatacacta ttagtaacca aactcctttc tggtgaattc ccggactttt cccctgtgat ctccactcat agcagtgtac aattagatct acatcgtgaa gaactgatct cccttctcaa acaagtggcc cttttcacta atgagtcttc gcattctgtc aaatttagct tctcacctgg agaactaact cttacagcca actgcacaaa agtaggagaa ggcaaggtaa gtatggctgt caattatact ggtgaaaccc tagaaatagc ctttaatcct ttcttcttct tagacatcct gaaacacagc cgtgatgaac ttgttcaatt aggtatctca gattcttaca atccaggaat cattacagac tccactcgca gcttgtttgt catcatgcct atgagattac atgacgatta a
Details for ChtrB.17987.a.B1.GE42420
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV NFRLHGAFSN PSFVKIFFTK ASPERRLTCF HVTKHGYSFQ TDLHDYRKIM KFVISRNELG NLIKKVQNVV PQSTPIPVLT HVLIESCNDE LVFTATDLTV STRCVVKAKV YESGSVTIPS RRFFQLIREL TEANIEVAAH SGEMATITSG SSCFRLLSMG KEDFPMLPDM QNALRFTLDS ERLKDMFQRT SFAVSREESR YVLTGVLLSI ANGTMTVVGT DGKRLAKIDT EISLDPSFSG DYIIPIKAVE EIIRMSSEDV QSTIFLDQTK IAVERGNTLL VTKLLSGEFP DFSPVISTHS SVQLDLHREE LISLLKQVAL FTNESSHSVK FSFSPGELTL TANCTKVGEG KVSMAVNYTG ETLEIAFNPF FFLDILKHSR DELVQLGISD SYNPGIITDS TRSLFV
Validated NT Sequence
gatgacaaac aagctgcgag tggagtctgt aatgattcct ggattgtaag aatctgagat acctaattga acaagttcat cacggctgtg tttcagaatg tctaagaaga agaaaggatt aaaggctatt tctagggttt caccagtata attgacagcc atacttacct tgccttctcc tacttttgtg cagttggctg taagagttag ttctccaggt gagaagctaa atttgacaga atgcgaagac tcattagtga aaagggccac ttgtttgaga agggagatca gttcttcacg atgtagatct aattgtacac tgctatgagt ggagatcaca ggggaaaagt ccgggaattc accagaaagg agtttggtta ctaatagtgt atttccacgt tcgacagcga ttttagtttg atctaagaaa atagtggatt gaacatcttc cgaagacatg cgaataattt cttcgacggc tttgataggg atgatatagt ctcctgaaaa actaggatct aaagaaatct ctgtatcgat cttagctaac cgtttcccat cagttcccac gactgtcata gttccattcg caatagaaag taaaacgcca gtgagtacat agcgactctc ttctctggat acagcaaaag aggttctttg gaacatgtct tttaatcgct cagaatccag agtgaaacgc agagcatttt gcatatcagg aagcataggg aaatcttctt tccccatact taacaggcga aaacaggaag aacccgacgt gattgtcgcc atttctccag aatgagctgc tacttcaata tttgcctctg ttaactcacg aatgagttgg aagaatcttc gagaagggat ggtaacagat ccagactcat agacctttgc tttaacaaca caacgagtgc tgaccgtcag gtctgtagcc gtgaaaacca actcatcgtt acagctttca atgagtacat gagtcaatac tggaattggc gtgctttgtg gaacgacatt ctgaactttt ttaattagat ttcctaattc attgcgggag ataacgaatt tcattatttt cctgtaatca tgtaagtctg tttggaatga ataaccatgt tttgtgacat ggaagcaagt aagtcgtctc tcggggctag ctttagtaaa aaatatcttg acaaaagacg gatttgaaaa agccccatga aggcggaaat tcaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN FRLHGAFSNP SFVKIFFTKA SPERRLTCFH VTKHGYSFQT DLHDYRKIMK FVISRNELGN LIKKVQNVVP QSTPIPVLTH VLIESCNDEL VFTATDLTVS TRCVVKAKVY ESGSVTIPSR RFFQLIRELT EANIEVAAHS GEMATITSGS SCFRLLSMGK EDFPMLPDMQ NALRFTLDSE RLKDMFQRTS FAVSREESRY VLTGVLLSIA NGTMTVVGTD GKRLAKIDTE ISLDPSFSGD YIIPIKAVEE IIRMSSEDVQ STIFLDQTKI AVECGNTLLV TKLLSGEFPD FSPVISTHSS VQLDLHREEL ISLLKQVALF TNESSHSVKF SFSPGELTLT ANCTKVGEGK VSMAVNYTGE TLEIAFNPFF FLDILKHSRD ELVQLGISDS YNPGIITDST RSLFVIMPMR LHDD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaatt tccgccttca tggggctttt tcaaatccgt cttttgtcaa gatatttttt actaaagcta gccccgagag acgacttact tgcttccatg tcacaaaaca tggttattca ttccaaacag acttacatga ttacaggaaa ataatgaaat tcgttatctc ccgcaatgaa ttaggaaatc taattaaaaa agttcagaat gtcgttccac aaagcacgcc aattccagta ttgactcatg tactcattga aagctgtaac gacgagttgg ttttcacggc tacagacctg acggtcagca ctcgttgtgt tgttaaagca aaggtctatg agtctggatc tgttaccatc ccttctcgaa gattcttcca actcattcgt gagttaacag aggcaaatat tgaagtagca gctcattctg gagaaatggc gacaatcacg tcgggttctt cctgttttcg cctgttaagt atggggaaag aagatttccc tatgcttcct gatatgcaaa atgctctgcg tttcactctg gattctgagc gattaaaaga catgttccaa agaacctctt ttgctgtatc cagagaagag agtcgctatg tactcactgg cgttttactt tctattgcga atggaactat gacagtcgtg ggaactgatg ggaaacggtt agctaagatc gatacagaga tttctttaga tcctagtttt tcaggagact atatcatccc tatcaaagcc gtcgaagaaa ttattcgcat gtcttcggaa gatgttcaat ccactatttt cttagatcaa actaaaatcg ctgtcgaatg tggaaataca ctattagtaa ccaaactcct ttctggtgaa ttcccggact tttcccctgt gatctccact catagcagtg tacaattaga tctacatcgt gaagaactga tctcccttct caaacaagtg gcccttttca ctaatgagtc ttcgcattct gtcaaattta gcttctcacc tggagaacta actcttacag ccaactgcac aaaagtagga gaaggcaagg taagtatggc tgtcaattat actggtgaaa ccctagaaat agcctttaat cctttcttct tcttagacat cctgaaacac agccgtgatg aacttgttca attaggtatc tcagattctt acaatccagg aatcattaca gactccactc gcagcttgtt tgtcatcatg cctatgagat tacatgacga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat