ChtrB.17981.a

Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase) (ProRS)

CENTER ID:	ChtrB.17981.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17981.a.B1.GE42419	full length		1	581

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17981.a.B1.PW38500	full length		1	581

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82029

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.423
UniProt:	P36431

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRMSLLFYRT SKNANKEASV LSYELLQKAG YLFKTSKGIY SYTPLFQRVI LKMTEIIREE LNAIGGQEVC LPLLQPAELW EKTGRWKAFL SEKLLYVLKD RENKAMCLAP THEEVVSEFV AQWLTGREQL PIHLYQIGTK FRDEIRPRFG LMRAKEFLME DSYTFSDSPE QMEEQYAKLR LAYQRIFDRL NLKYVIVAAD GGKIGKGKSE EFHVLCSLGE DTICVSGSYG ANVEAAQAIP PSYVYDSNLL PVEEVATPNI RTIEDLEVFF NTPKHKILKT LVVKTCQKDS EKFFAICIRG DRQINLTKVA SFLQVDDCEL ASEEEILKHL HVEKGFIGPL YCPIPCYADE TTRPMTNFIC ANNQKDVHCK HVNWGRDIPL PAFGDFLLAE AGDLCPQNGG APYEIFQGVE VAHIFNLGTR YTESFSVGFQ DKNGDKQLCW MGTYGIGVGR TLAACIEQLA DNKGLVWPLA VAPFSITILY NGGDTEGEAT ALQLYQSLNT EGFEPLLDDR NERLGFKLKD SDLLGIPYKL IIGKSFQSTG LLEIESRSGE KCNVSPENLL DWCSKNLPCH TRKIPPLREQ N

NT Sequence

atgagaatgt ccctcctgtt ttacagaact tccaaaaatg caaataaaga agcctccgtt ctttcctacg agcttcttca gaaagcaggg tatcttttca aaacatctaa agggatttac tcatacacac ctctcttcca acgagttatc ctaaaaatga ccgaaatcat tcgtgaagaa ctcaatgcta ttggaggaca agaggtctgt ctccctctcc tgcaaccagc agaactatgg gaaaaaaccg gtcgttggaa ggccttttta tcggaaaaat tactctatgt gcttaaggat agagaaaata aggcaatgtg tttagcccct acccacgaag aggttgtctc tgaattcgtg gctcagtggc taacaggacg agaacagctc cctatccacc tataccaaat cggaaccaaa ttccgagatg aaatccgacc tcgctttggg cttatgcgtg ccaaagagtt cttaatggaa gatagctata ctttttctga ctctccagaa caaatggaag aacagtatgc aaagctacgt ctagcttacc agcgcatctt tgatcgatta aatctcaaat atgtaattgt tgctgcagat ggagggaaaa ttggtaaggg gaaatctgag gaattccatg ttctctgctc tttaggagaa gacacgatct gtgtgagtgg gtcttacgga gcgaatgtag aagcagcaca agcaatccct ccatcctatg tttacgattc aaaccttcta cctgtagaag aagttgcgac tcctaacatt cgaacaattg aagacttaga agtctttttt aacaccccta aacataaaat actgaaaact ttagttgtta aaacttgcca aaaagactcg gagaaattct ttgcgatttg tatccgaggg gatcgtcaga tcaacttaac caaagttgca tctttcctac aagttgatga ctgcgagctg gcttctgagg aagagatcct taagcatctt catgttgaaa aaggatttat cggacctcta tactgtccta tcccctgtta tgcagacgaa acaacgcgcc caatgactaa cttcatctgc gcaaacaacc aaaaagacgt gcactgcaag catgtgaact ggggacgcga tatccctctt ccagctttcg gagactttct tttagcagaa gctggagacc tttgtcctca aaatggagga gctccctatg aaattttcca aggcgtcgaa gtcgctcata tcttcaatct gggaacccgc tatacggaga gcttctccgt agggttccag gataaaaatg gagataagca gctttgctgg atggggacat atggtattgg agtagggaga acccttgctg catgcataga acagctcgct gataacaaag gactggtttg gcctttagct gtagctcctt tctccatcac cattctctat aatggtggtg atacggaagg agaagccacc gccttacaac tttatcaaag cctgaatact gaaggctttg agcctctact agatgatcgc aatgaacgcc ttgggtttaa gttaaaagac agcgatttac taggtatccc gtacaaattg atcattggga agtctttcca aagtacaggc ttattagaaa tcgaatcacg ctcaggagaa aaatgtaatg tttctcctga aaacctattg gattggtgct ctaaaaatct tccttgtcat acaagaaaga tccctccact ccgagaacaa aactag

Details for ChtrB.17981.a.B1.GE42419

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR MSLLFYRTSK NANKEASVLS YELLQKAGYL FKTSKGIYSY TPLFQRVILK MTEIIREELN AIGGQEVCLP LLQPAELWEK TGRWKAFLSE KLLYVLKDRE NKAMCLAPTH EEVVSEFVAQ WLTGREQLPI HLYQIGTKFR DEIRPRFGLM RAKEFLMEDS YTFSDSPEQM EEQYAKLRLA YQRIFDRLNL KYVIVAADGG KIGKGKSEEF HVLCSLGEDT ICVSGSYGAN VEAAQAIPPS YVYDSNLLPV XXVATPNIRT IXXLEVFFNT PKHKILKTLV VKTRQKDSEK FFAICIRGDR QINLTKVASF LQVDDCELAS EEEILKHLHV EKGFIGPLYC PIPCYADETT RPMTNFICAN NQKDVHCKHV NWGRDIPLPA FGDFLLAEAG DLCPQNGGAP YEIFQGVEVA HIFNLGTRYT ESFSVGFQDK NGDKQLCWMG TYGIGVGRTL AACIEQLADN KGLVWPLAVA PFSITILYNG GDTEGEATAL QLYQSLNTEG FEPLLDDRNE RLGFKLKDSD LLGIPYKLII GKSFQSTGLL EIESRSGEKC NVSPENLLDW CSKNLPCHTR K

Validated NT Sequence

gatctttctt gtatgacaag gaagattttt agagcaccaa tccaataggt tttcaggaga aacattacat ttttctcctg agcgtgattc gatttctaat aagcctgtac tttggaaaga cttcccgatg atcaatttgt acgggatacc tagtaaatcg ctgtctttta acttaaaccc aaggcgttca ttgcgatcat ctagtagagg ctcaaagcct tcagtattca ggctttgata aagttgtaag gcggtggctt ctccttccgt atcaccaccg ttatagagaa tggtgatgga gaaaggagct acagctaaag gccaaaccag tcctttgtta tcagcgagct gttctatgca tgcagcaagg gttctcccta ctccaatacc atatgtcccc atccagcaaa gctgtttatc tccattttta tcctggaacc ctacggagaa gctctccgta tagcgggttc ccagattgaa gatatgagcg acttcgacgc cttggaaaat ttcataggga gctcctccat tttgaggaca aaggtctcca gcttctgcta aaagaaagtc tccgaaagct ggaagaggga tatcgcgtcc ccagttcaca tgcttgcagt gcacgtcttt ttggttgttt gcgcagatga agttagtcat tgggcgcgtt gtttcgtctg cataacaggg gataggacag tatagaggtc cgataaatcc tttttcaaca tgaagatgct taaggatctc ttcctcagaa gccagctcgc agtcatcaac ttgtaggaaa gatgcaactt tggttaagtt gatctgacga tcccctcgga tacaaatcgc aaagaatttc tccgagtctt tttggcgagt tttaacaact aaagttttca gtattttatg tttaggggtg ttaaaaaaga cttctaagnn ntcaattgtt cgaatgttag gagtcgcaac tnnntctaca ggtagaaggt ttgaatcgta aacataggat ggagggattg cttgtgctgc ttctacattc gctccgtaag acccactcac acagatcgtg tcttctccta aagaacagag aacatggaat tcctcagatt tccccttacc aattttcccc ccatctgcag caacaattac atatttgaga tttaatcgat caaagatgcg ctggtaagct agacgtagct ttgcatactg ttcttccatt tgttctggag agtcagaaaa agtatagcta tcttccatta agaactcttt ggcacgcata agcccaaagc gaggtcggat ttcatctcgg aatttggttc cgatttggta taggtggata gggagctgtt ctcgtcctgt tagccactga gccacgaatt cagagacaac ctcttcgtgg gtaggggcta aacacattgc cttattttct ctatccttaa gcacatagag taatttttcc gataagaagg ccttccaacg accggttttt tcccatagtt ctgctggttg caggagaggg agacagacct cttgtcctcc aatagcattg agttcttcac gaatgatttc ggtcattttt aggataactc gttggaagag aggtgtgtat gagtaaatcc ctttagatgt tttgaaaaga taccctgctt tctgaagaag ctcgtaggaa agaacggagg cttctttatt tgcatttttg gaagttctgt aaaacaggag ggacattctc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMR MSLLFYRTSK NANKEASVLS YELLQKAGYL FKTSKGIYSY TPLFQRVILK MTEIIREELN AIGGQEVCLP LLQPAELWEK TGRWKAFLSE KLLYVLKDRE NKAMCLAPTH EEVVSEFVAQ WLTGREQLPI HLYQIGTKFR DEIRPRFGLM RAKEFLMEDS YTFSDSPEQM EEQYAKLRLA YQRIFDRLNL KYVIVAADGG KIGKGKSEEF HVLCSLGEDT ICVSGSYGAN VEAAQAIPPS YVYDSNLLPV EEVATPNIRT IEDLEVFFNT PKHKILKTLV VKTCQKDSEK FFAICIRGDR QINLTKVASF LQVDDCELAS EEEILKHLHV EKGFIGPLYC PIPCYADETT RPMTNFICAN NQKDVHCKHV NWGRDIPLPA FGDFLLAEAG DLCPQNGGAP YEIFQGVEVA HIFNLGTRYT ESFSVGFQDK NGDKQLCWMG TYGIGVGRTL AACIEQLADN KGLVWPLAVA PFSITILYNG GDTEGEATAL QLYQSLNTEG FEPLLDDRNE RLGFKLKDSD LLGIPYKLII GKSFQSTGLL EIESRSGEKC NVSPENLLDW CSKNLPCHTR KIPPLREQN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa tgtccctcct gttttacaga acttccaaaa atgcaaataa agaagcctcc gttctttcct acgagcttct tcagaaagca gggtatcttt tcaaaacatc taaagggatt tactcataca cacctctctt ccaacgagtt atcctaaaaa tgaccgaaat cattcgtgaa gaactcaatg ctattggagg acaagaggtc tgtctccctc tcctgcaacc agcagaacta tgggaaaaaa ccggtcgttg gaaggccttt ttatcggaaa aattactcta tgtgcttaag gatagagaaa ataaggcaat gtgtttagcc cctacccacg aagaggttgt ctctgaattc gtggctcagt ggctaacagg acgagaacag ctccctatcc acctatacca aatcggaacc aaattccgag atgaaatccg acctcgcttt gggcttatgc gtgccaaaga gttcttaatg gaagatagct atactttttc tgactctcca gaacaaatgg aagaacagta tgcaaagcta cgtctagctt accagcgcat ctttgatcga ttaaatctca aatatgtaat tgttgctgca gatggaggga aaattggtaa ggggaaatct gaggaattcc atgttctctg ctctttagga gaagacacga tctgtgtgag tgggtcttac ggagcgaatg tagaagcagc acaagcaatc cctccatcct atgtttacga ttcaaacctt ctacctgtag aagaagttgc gactcctaac attcgaacaa ttgaagactt agaagtcttt tttaacaccc ctaaacataa aatactgaaa actttagttg ttaaaacttg ccaaaaagac tcggagaaat tctttgcgat ttgtatccga ggggatcgtc agatcaactt aaccaaagtt gcatctttcc tacaagttga tgactgcgag ctggcttctg aggaagagat ccttaagcat cttcatgttg aaaaaggatt tatcggacct ctatactgtc ctatcccctg ttatgcagac gaaacaacgc gcccaatgac taacttcatc tgcgcaaaca accaaaaaga cgtgcactgc aagcatgtga actggggacg cgatatccct cttccagctt tcggagactt tcttttagca gaagctggag acctttgtcc tcaaaatgga ggagctccct atgaaatttt ccaaggcgtc gaagtcgctc atatcttcaa tctgggaacc cgctatacgg agagcttctc cgtagggttc caggataaaa atggagataa gcagctttgc tggatgggga catatggtat tggagtaggg agaacccttg ctgcatgcat agaacagctc gctgataaca aaggactggt ttggccttta gctgtagctc ctttctccat caccattctc tataatggtg gtgatacgga aggagaagcc accgccttac aactttatca aagcctgaat actgaaggct ttgagcctct actagatgat cgcaatgaac gccttgggtt taagttaaaa gacagcgatt tactaggtat cccgtacaaa ttgatcattg ggaagtcttt ccaaagtaca ggcttattag aaatcgaatc acgctcagga gaaaaatgta atgtttctcc tgaaaaccta ttggattggt gctctaaaaa tcttccttgt catacaagaa agatccctcc actccgagaa caaaactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for ChtrB.17981.a.B1.PW38500

PURIFICATION DATe:	6/25/2018
CONCENTRATION:	17.78mg/ml
OBSERVED MW:	67kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMR MSLLFYRTSK NANKEASVLS YELLQKAGYL FKTSKGIYSY TPLFQRVILK MTEIIREELN AIGGQEVCLP LLQPAELWEK TGRWKAFLSE KLLYVLKDRE NKAMCLAPTH EEVVSEFVAQ WLTGREQLPI HLYQIGTKFR DEIRPRFGLM RAKEFLMEDS YTFSDSPEQM EEQYAKLRLA YQRIFDRLNL KYVIVAADGG KIGKGKSEEF HVLCSLGEDT ICVSGSYGAN VEAAQAIPPS YVYDSNLLPV EEVATPNIRT IEDLEVFFNT PKHKILKTLV VKTCQKDSEK FFAICIRGDR QINLTKVASF LQVDDCELAS EEEILKHLHV EKGFIGPLYC PIPCYADETT RPMTNFICAN NQKDVHCKHV NWGRDIPLPA FGDFLLAEAG DLCPQNGGAP YEIFQGVEVA HIFNLGTRYT ESFSVGFQDK NGDKQLCWMG TYGIGVGRTL AACIEQLADN KGLVWPLAVA PFSITILYNG GDTEGEATAL QLYQSLNTEG FEPLLDDRNE RLGFKLKDSD LLGIPYKLII GKSFQSTGLL EIESRSGEKC NVSPENLLDW CSKNLPCHTR KIPPLREQN

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAA TGTCCCTCCT GTTTTACAGA ACTTCCAAAA ATGCAAATAA AGAAGCCTCC GTTCTTTCCT ACGAGCTTCT TCAGAAAGCA GGGTATCTTT TCAAAACATC TAAAGGGATT TACTCATACA CACCTCTCTT CCAACGAGTT ATCCTAAAAA TGACCGAAAT CATTCGTGAA GAACTCAATG CTATTGGAGG ACAAGAGGTC TGTCTCCCTC TCCTGCAACC AGCAGAACTA TGGGAAAAAA CCGGTCGTTG GAAGGCCTTT TTATCGGAAA AATTACTCTA TGTGCTTAAG GATAGAGAAA ATAAGGCAAT GTGTTTAGCC CCTACCCACG AAGAGGTTGT CTCTGAATTC GTGGCTCAGT GGCTAACAGG ACGAGAACAG CTCCCTATCC ACCTATACCA AATCGGAACC AAATTCCGAG ATGAAATCCG ACCTCGCTTT GGGCTTATGC GTGCCAAAGA GTTCTTAATG GAAGATAGCT ATACTTTTTC TGACTCTCCA GAACAAATGG AAGAACAGTA TGCAAAGCTA CGTCTAGCTT ACCAGCGCAT CTTTGATCGA TTAAATCTCA AATATGTAAT TGTTGCTGCA GATGGAGGGA AAATTGGTAA GGGGAAATCT GAGGAATTCC ATGTTCTCTG CTCTTTAGGA GAAGACACGA TCTGTGTGAG TGGGTCTTAC GGAGCGAATG TAGAAGCAGC ACAAGCAATC CCTCCATCCT ATGTTTACGA TTCAAACCTT CTACCTGTAG AAGAAGTTGC GACTCCTAAC ATTCGAACAA TTGAAGACTT AGAAGTCTTT TTTAACACCC CTAAACATAA AATACTGAAA ACTTTAGTTG TTAAAACTTG CCAAAAAGAC TCGGAGAAAT TCTTTGCGAT TTGTATCCGA GGGGATCGTC AGATCAACTT AACCAAAGTT GCATCTTTCC TACAAGTTGA TGACTGCGAG CTGGCTTCTG AGGAAGAGAT CCTTAAGCAT CTTCATGTTG AAAAAGGATT TATCGGACCT CTATACTGTC CTATCCCCTG TTATGCAGAC GAAACAACGC GCCCAATGAC TAACTTCATC TGCGCAAACA ACCAAAAAGA CGTGCACTGC AAGCATGTGA ACTGGGGACG CGATATCCCT CTTCCAGCTT TCGGAGACTT TCTTTTAGCA GAAGCTGGAG ACCTTTGTCC TCAAAATGGA GGAGCTCCCT ATGAAATTTT CCAAGGCGTC GAAGTCGCTC ATATCTTCAA TCTGGGAACC CGCTATACGG AGAGCTTCTC CGTAGGGTTC CAGGATAAAA ATGGAGATAA GCAGCTTTGC TGGATGGGGA CATATGGTAT TGGAGTAGGG AGAACCCTTG CTGCATGCAT AGAACAGCTC GCTGATAACA AAGGACTGGT TTGGCCTTTA GCTGTAGCTC CTTTCTCCAT CACCATTCTC TATAATGGTG GTGATACGGA AGGAGAAGCC ACCGCCTTAC AACTTTATCA AAGCCTGAAT ACTGAAGGCT TTGAGCCTCT ACTAGATGAT CGCAATGAAC GCCTTGGGTT TAAGTTAAAA GACAGCGATT TACTAGGTAT CCCGTACAAA TTGATCATTG GGAAGTCTTT CCAAAGTACA GGCTTATTAG AAATCGAATC ACGCTCAGGA GAAAAATGTA ATGTTTCTCC TGAAAACCTA TTGGATTGGT GCTCTAAAAA TCTTCCTTGT CATACAAGAA AGATCCCTCC ACTCCGAGAA CAAAACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T