ChtrB.17946.a

DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD(+)])

CENTER ID:	ChtrB.17946.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17946.a.B1.GE42418	full length		1	663

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17946.a.B1.PW38611	full length		1	663

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82028
de-novo with evolutionary constraints	ModelArchive_ma-ifaip

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.159
UniProt:	O84148

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAVSRDDYI ALCTELVEHD RRYYVLNQPT ISDYSYDVKM RELQEIEVQH PEWKVSWSPT MYLGDRPSGQ FPVVPHSSPM LSIANVYSLQ ELEEFFSRTE KLLGYSPGYS LELKIDGIAV AIRYEKRLFA QALSRGNGVK GEDITANVST IRSLPMRLPQ EAPEDLEVRG EVFLSYEAFE ELNACQREQG KLEFANPRNA AGGTLKLLSS KEAAKRKLDL SVYGLITDQK KRSHFENLQL CSQWGFFVAG MPKQCRSRQD VVERIREIEE MRAALPMAID GVVIKVDNIA YQDRLGLTSK HYRWAIAYKY APERAETILE DIVVQVGKTG ILTPVAELAP VFLSGSRVSR ASLYNQDEIE KKDIRIGDSV YVEKGGEVIP KIVGINLAKR SLESEPWKMP SLCPVCHEPV VKEKVSVRCV NPLCSGGMLE KICFFASKSA LNIDHLGEKV VTKLFEVGLI SSCSDIFALA EEDLKQVPGF KDRSIQNLLA SIAGAKKVAL DRLLTALSIP FVGSSGAIAL ADHFGTLDKV IEASLDELMS IEGIGPKVAA SIVAFFSKHE NREEIRRMQE LGVQVLSKQS DKEAPLQGKV FVLTGTLQQM TRTQAEERIR CLGGKVSSSV SKSTYAVIAG SEAGGKLKKA QDLGLSIWNE SELLRILDAK SVS

NT Sequence

atgggtgctg tatctcgaga tgattatatt gctttatgca cagaattagt ggaacatgac cgacgctatt atgtgctcaa tcaaccgacg atttctgatt acagctatga tgtgaaaatg cgagagcttc aagaaataga agttcagcat ccggaatgga aggtttcatg gtctcctacc atgtatttgg gggatcgtcc ttcaggacag tttcctgtgg ttcctcattc tagccccatg ttgtctatag cgaatgtata ctcattgcaa gagctagagg aatttttttc ccgtacagaa aaattattgg ggtactctcc tggatattct ttagaactca aaattgatgg aattgctgtc gccattcggt acgagaagcg attgtttgct caagctttga gtcgggggaa tggggtaaaa ggagaagata ttacagctaa tgtcagtact atacgctctt tacctatgag actccctcaa gaggctcccg aggatctaga agtgcgagga gaagtgtttc tttcatatga agcgtttgaa gagctcaatg cttgtcaacg ggaacaaggg aaactggaat ttgctaatcc tcgtaatgca gctggaggaa ctcttaagct tttatcttct aaggaagctg ctaagcgtaa attagacttg tctgtttatg ggttgatcac agatcaaaaa aaacgctcac attttgaaaa tcttcagtta tgctctcaat ggggattttt cgttgcaggg atgccgaaac aatgccgttc gagacaagat gtggtagaac gtattcgaga gatagaggag atgcgcgcag cgctcccgat ggctatcgat ggagtcgtca ttaaagtaga caatattgca taccaggatc ggctaggttt gacgagcaaa cattatcgtt gggcgatagc ttataaatat gctccggaaa gagcagaaac gattttggag gatattgtcg ttcaagtagg gaaaacgggt attcttactc cggttgccga gttagctcct gtttttttat ctgggagccg cgtgtccaga gcatccttat acaaccaaga tgagattgaa aaaaaagata ttcgcatcgg agattcggtg tatgtcgaaa aaggaggaga ggttattccc aaaattgttg ggattaattt ggctaagcgt tcattagaga gcgagccttg gaaaatgcca agcctgtgtc ctgtatgtca tgaacctgtt gttaaagaga aggtgtcggt gcgttgtgtc aatccacttt gttcaggggg gatgctagaa aagatctgtt tctttgcaag taagagcgct ttaaacatag atcatttggg agagaaggtc gtaactaaat tattcgaagt aggtctcatt agttcttgtt cggatatatt tgctcttgca gaagaagatt tgaaacaggt ccctgggttt aaagatcgtt ctatacagaa tttattggca agcattgccg gagccaaaaa ggtggcatta gatcggcttt taacggctct ttctattcct tttgtgggga gctctggtgc catagctttg gcagaccact tcggaactct ggacaaagta atcgaagctt ctttagatga gctgatgtct atagagggga ttggtcctaa ggtagctgca tcgatcgtcg cgttcttctc gaaacatgag aatagagagg aaattcgtcg gatgcaagag ctaggagttc aggttctttc taagcaatcc gataaagagg ctcctttaca aggaaaagtg tttgttctta cggggactct ccaacagatg acgagaacgc aagcagagga gcgtatccgc tgcctagggg ggaaagtgag ctcttcggta tcgaagagca catatgccgt catagcaggt agcgaagctg ggggtaaatt gaaaaaagct caagatttag gtttaagtat ttggaacgag agcgaattat taagaattct tgatgcaaaa tcggtctcct ga

Details for ChtrB.17946.a.B1.GE42418

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	75kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG AVSRDDYIAL CTELVEHDRR YYVLNQPTIS DYSYDVKMRE LQEIEVQHPE WKVSWSPTMY LGDRPSGQFP VVPHSSPMLS IANVYSLQEL EEFFSRTEKL LGYSPGYSLE LKIDGIAVAI RYEKRLFAQA LSRGNGVKGE DITANVSTIR SLPMRLPQEA PEDLEVRGEV FLSYEAFEEL NACQREQGKL EFANPRNAAG GTLKLLSSKE AAKRKLDLSV YGLITDQKKR SHFENLQLCS QWGFFVAGMP KQCRSRQDVV ERIREIEEMR AALPMAIDGV VIKVDNIAYQ DRLGLTSKHY RWAIAYKYAP ERAETILEDI VVQVGKTGIL TPVAELAPVF LSGSRVSRAS LYNQDEIEKK DIRIGDSVYV EKGGEVIPKI VGINLAKRSL ESEPWKMPSL CPVCHEPVVK EKVSVRCVNP LCSGGMLEKI CFFASKSALN IDHLGEKVVT KLFEVGLISS CSDIFALAEE DLKQVPGFKD RSIQNLLASI AGAKKVALDR LLTALSIPFV GSSGAIALAD HFGTLDKVIE ASLDELMSIE GIGPKVAASI VAFFSKHENR EEIRRMQELG VQVLSKQSDK EAPLQGKVFV LTGTLQQMTR TQAEERIRCL GGKVSSSVSK STYAVIAGSE AGGKLKKAQD LGLSIWNESE LLRILDAKSV S

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg ctgtatctcg agatgattat attgctttat gcacagaatt agtggaacat gaccgacgct attatgtgct caatcaaccg acgatttctg attacagcta tgatgtgaaa atgcgagagc ttcaagaaat agaagttcag catccggaat ggaaggtttc atggtctcct accatgtatt tgggggatcg tccttcagga cagtttcctg tggttcctca ttctagcccc atgttgtcta tagcgaatgt atactcattg caagagctag aggaattttt ttcccgtaca gaaaaattat tggggtactc tcctggatat tctttagaac tcaaaattga tggaattgct gtcgccattc ggtacgagaa gcgattgttt gctcaagctt tgagtcgggg gaatggggta aaaggagaag atattacagc taatgtcagt actatacgct ctttacctat gagactccct caagaggctc ccgaggatct agaagtgcga ggagaagtgt ttctttcata tgaagcgttt gaagagctca atgcttgtca acgggaacaa gggaaactgg aatttgctaa tcctcgtaat gcagctggag gaactcttaa gcttttatct tctaaggaag ctgctaagcg taaattagac ttgtctgttt atgggttgat cacagatcaa aaaaaacgct cacattttga aaatcttcag ttatgctctc aatggggatt tttcgttgca gggatgccga aacaatgccg ttcgagacaa gatgtggtag aacgtattcg agagatagag gagatgcgcg cagcgctccc gatggctatc gatggagtcg tcattaaagt agacaatatt gcataccagg atcggctagg tttgacgagc aaacattatc gttgggcgat agcttataaa tatgctccgg aaagagcaga aacgattttg gaggatattg tcgttcaagt agggaaaacg ggtattctta ctccggttgc cgagttagct cctgtttttt tatctgggag ccgcgtgtcc agagcatcct tatacaacca agatgagatt gaaaaaaaag atattcgcat cggagattcg gtgtatgtcg aaaaaggagg agaggttatt cccaaaattg ttgggattaa tttggctaag cgttcattag agagcgagcc ttggaaaatg ccaagcctgt gtcctgtatg tcatgaacct gttgttaaag agaaggtgtc ggtgcgttgt gtcaatccac tttgttcagg ggggatgcta gaaaagatct gtttctttgc aagtaagagc gctttaaaca tagatcattt gggagagaag gtcgtaacta aattattcga agtaggtctc attagttctt gttcggatat atttgctctt gcagaagaag atttgaaaca ggtccctggg tttaaagatc gttctataca gaatttattg gcaagcattg ccggagccaa aaaggtggca ttagatcggc ttttaacggc tctttctatt ccttttgtgg ggagctctgg tgccatagct ttggcagacc acttcggaac tctggacaaa gtaatcgaag cttctttaga tgagctgatg tctatagagg ggattggtcc taaggtagct gcatcgatcg tcgcgttctt ctcgaaacat gagaatagag aggaaattcg tcggatgcaa gagctaggag ttcaggttct ttctaagcaa tccgataaag aggctccttt acaaggaaaa gtgtttgttc ttacggggac tctccaacag atgacgagaa cgcaagcaga ggagcgtatc cgctgcctag gggggaaagt gagctcttcg gtatcgaaga gcacatatgc cgtcatagca ggtagcgaag ctgggggtaa attgaaaaaa gctcaagatt taggtttaag tatttggaac gagagcgaat tattaagaat tcttgatgca aaatcggtct cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for ChtrB.17946.a.B1.PW38611

PURIFICATION DATe:	5/1/2019
CONCENTRATION:	19.5mg/ml
OBSERVED MW:	78kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	55µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTG CTGTATCTCG AGATGATTAT ATTGCTTTAT GCACAGAATT AGTGGAACAT GACCGACGCT ATTATGTGCT CAATCAACCG ACGATTTCTG ATTACAGCTA TGATGTGAAA ATGCGAGAGC TTCAAGAAAT AGAAGTTCAG CATCCGGAAT GGAAGGTTTC ATGGTCTCCT ACCATGTATT TGGGGGATCG TCCTTCAGGA CAGTTTCCTG TGGTTCCTCA TTCTAGCCCC ATGTTGTCTA TAGCGAATGT ATACTCATTG CAAGAGCTAG AGGAATTTTT TTCCCGTACA GAAAAATTAT TGGGGTACTC TCCTGGATAT TCTTTAGAAC TCAAAATTGA TGGAATTGCT GTCGCCATTC GGTACGAGAA GCGATTGTTT GCTCAAGCTT TGAGTCGGGG GAATGGGGTA AAAGGAGAAG ATATTACAGC TAATGTCAGT ACTATACGCT CTTTACCTAT GAGACTCCCT CAAGAGGCTC CCGAGGATCT AGAAGTGCGA GGAGAAGTGT TTCTTTCATA TGAAGCGTTT GAAGAGCTCA ATGCTTGTCA ACGGGAACAA GGGAAACTGG AATTTGCTAA TCCTCGTAAT GCAGCTGGAG GAACTCTTAA GCTTTTATCT TCTAAGGAAG CTGCTAAGCG TAAATTAGAC TTGTCTGTTT ATGGGTTGAT CACAGATCAA AAAAAACGCT CACATTTTGA AAATCTTCAG TTATGCTCTC AATGGGGATT TTTCGTTGCA GGGATGCCGA AACAATGCCG TTCGAGACAA GATGTGGTAG AACGTATTCG AGAGATAGAG GAGATGCGCG CAGCGCTCCC GATGGCTATC GATGGAGTCG TCATTAAAGT AGACAATATT GCATACCAGG ATCGGCTAGG TTTGACGAGC AAACATTATC GTTGGGCGAT AGCTTATAAA TATGCTCCGG AAAGAGCAGA AACGATTTTG GAGGATATTG TCGTTCAAGT AGGGAAAACG GGTATTCTTA CTCCGGTTGC CGAGTTAGCT CCTGTTTTTT TATCTGGGAG CCGCGTGTCC AGAGCATCCT TATACAACCA AGATGAGATT GAAAAAAAAG ATATTCGCAT CGGAGATTCG GTGTATGTCG AAAAAGGAGG AGAGGTTATT CCCAAAATTG TTGGGATTAA TTTGGCTAAG CGTTCATTAG AGAGCGAGCC TTGGAAAATG CCAAGCCTGT GTCCTGTATG TCATGAACCT GTTGTTAAAG AGAAGGTGTC GGTGCGTTGT GTCAATCCAC TTTGTTCAGG GGGGATGCTA GAAAAGATCT GTTTCTTTGC AAGTAAGAGC GCTTTAAACA TAGATCATTT GGGAGAGAAG GTCGTAACTA AATTATTCGA AGTAGGTCTC ATTAGTTCTT GTTCGGATAT ATTTGCTCTT GCAGAAGAAG ATTTGAAACA GGTCCCTGGG TTTAAAGATC GTTCTATACA GAATTTATTG GCAAGCATTG CCGGAGCCAA AAAGGTGGCA TTAGATCGGC TTTTAACGGC TCTTTCTATT CCTTTTGTGG GGAGCTCTGG TGCCATAGCT TTGGCAGACC ACTTCGGAAC TCTGGACAAA GTAATCGAAG CTTCTTTAGA TGAGCTGATG TCTATAGAGG GGATTGGTCC TAAGGTAGCT GCATCGATCG TCGCGTTCTT CTCGAAACAT GAGAATAGAG AGGAAATTCG TCGGATGCAA GAGCTAGGAG TTCAGGTTCT TTCTAAGCAA TCCGATAAAG AGGCTCCTTT ACAAGGAAAA GTGTTTGTTC TTACGGGGAC TCTCCAACAG ATGACGAGAA CGCAAGCAGA GGAGCGTATC CGCTGCCTAG GGGGGAAAGT GAGCTCTTCG GTATCGAAGA GCACATATGC CGTCATAGCA GGTAGCGAAG CTGGGGGTAA ATTGAAAAAA GCTCAAGATT TAGGTTTAAG TATTTGGAAC GAGAGCGAAT TATTAAGAAT TCTTGATGCA AAATCGGTCT CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT