ChtrB.17901.a

Peptide chain release factor 1 (RF-1)

CENTER ID:	ChtrB.17901.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17901.a.B1.GE42416	full length		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17901.a.B1.PW38525	full length		1	359

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82026

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.28
UniProt:	O84026

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEIKVLECLK RLEEVEKQIS DPNIFSNPKE YSSLSKEHAR LSEIKNAHES LVATKKILQD DKLALSTEKD PEIVAMLEEG VLVGEEAVER LSKQLENLLI PPDPDDDLSV IMELRAGTGG DEAALFVGDC VRMYHLYAAS KGWQCEVLST SESDLGGYKE YVMGISGASV KRFLQYEAGT HRVQRVPETE TQGRVHTSAV TVAVLPEPAE DDEEVFIDEK DLRIDTFRSS GAGGQHVNVT DSAVRITHIP SGVVVTCQDE RSQHKNKAKA MRVLKARIRD AEVQKRAQEA SAMRSAQVGS GDRSERIRTY NFPQNRVTDH RIGLTLYNLD RVMEGELDMI TTALVTHVHR QLFGHEETA

NT Sequence

atggaaataa aagttttaga gtgtttaaag cgccttgaag aagttgaaaa gcagatatcc gatccgaata tctttagtaa tcctaaagaa tatagttcgc tgagcaagga gcatgcgcgt ctttctgaga ttaaaaatgc tcatgagtca ttggttgcga caaagaaaat tcttcaggac gataaactcg ctttatcaac agagaaggat ccagaaatag tagctatgct agaagaagga gttcttgtag gggaagaggc tgtagaacgt ctatcgaagc agttagaaaa cctgcttatt ccacctgatc cagatgatga tctcagtgtg attatggagt tgcgagcagg aacgggagga gatgaagcgg ctctttttgt aggggactgt gtgcgcatgt atcaccttta tgcagcaagt aaggggtggc aatgcgaagt tctctctaca tcggagtcag atctcggagg ctacaaagaa tatgttatgg ggatttctgg ggcttctgtg aaacgtttct tgcagtatga agcaggaaca catcgtgtgc aaagggtccc agaaacagag actcagggta gggtacatac gtctgcggta acggtagctg ttcttccaga accagcagaa gatgacgaag aagttttcat tgatgagaag gatttacgta ttgatacctt ccgttcttct ggagccggag gccagcacgt caacgttaca gattccgctg tgcgtattac tcatattcct tctggcgttg tcgttacgtg ccaagatgaa cgcagtcagc ataaaaataa agctaaggct atgcgcgtgc taaaagctcg tattcgcgat gcagaagtgc agaagcgcgc gcaagaagcc tctgctatgc gttctgctca ggtaggaagc ggagatcgtt cggagcgaat tcgaacctat aattttcctc aaaaccgtgt gaccgatcac cgaattggct taactttata taacttagat cgtgtaatgg aaggagagtt ggatatgatt acgacagctc ttgtaaccca cgtacatcgg cagctattcg gtcatgaaga aactgcttag

Details for ChtrB.17901.a.B1.GE42416

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHME IKVLECLKRL EEVEKQISDP NIFSNPKEYS SLSKEHARLS EIKNAHESLV ATKKILQDDK LALSTEKDPE IVAMLEEGVL VGEEAVERLS KQLENLLIPP DPDDDLSVIM ELRAGTGGDE AALFVGDCVR MYHLYAASKG WQCEVLSASE SDLGGYKEYV MGISGASVKR FLQYEAGTHR VQRVPETETQ GRVHTSAVTV AVLPEPAEDD EEVFIDEKDL RIDTFRSSGA GGQHVNVTDS AVRITHIPSG VVVTCQDERS QHKNKAKAMR VLKARIRDAE VQKRAQEASA MRSAQVGSGD RSERIRTYNF PQNRVTDHRI GLTLYNLDRV MEGELDMITT ALVTHVHRQ

Validated NT Sequence

tagctgccga tgtacgtggg ttacaagagc tgtcgtaatc atatccaact ccccttccat tacacgatct aagttatata aagttaagcc aattcggtga tcggtcacac ggttttgagg aaaattatag gttcgaattc gctccgaacg atctccgctt cctacctgag cagaacgcat agcagaggct tcttgcgcgc gcttctgcac ttctgcatcg cgaatacgag cttttagcac gcgcatagcc ttagctttat ttttatgctg actgcgttca tcttggcacg taacgacaac gccagaagga atatgagtaa tacgcacagc ggaatctgta acgttgacgt gctggcctcc ggctccagaa gaacggaagg tatcaatacg taaatccttc tcatcaatga aaacttcttc gtcatcttct gctggttctg gaagaacagc taccgttacc gcagacgtat gtaccctacc ctgagtctct gtttctggga ccctttgcac acgatgtgtt cctgcttcat actgcaagaa acgtttcaca gaagccccag aaatccccat aacatattct ttgtagcctc cgagatctga ctccgatgca gagagaactt cgcattgcca ccccttactt gctgcataaa ggtgatacat gcgcacacag tcccctacaa aaagagccgc ttcatctcct cccgttcctg ctcgcaactc cataatcaca ctgagatcat catctggatc aggtggaata agcaggtttt ctaactgctt cgataggcgt tctacagcct cttcccctac aagaactcct tcttctagca tagctactat ttctggatcc ttctctgttg ataaagcgag tttatcgtcc tgaagaattt tctttgtcgc aaccaatgac tcatgagcat ttttaatctc agaaagacgc gcatgctcct tgctcagcga actatattct ttaggattac taaagatatt cggatcggat atctgctttt caacttcttc aaggcgcttt aaacactcta aaacttttat ttccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHME IKVLECLKRL EEVEKQISDP NIFSNPKEYS SLSKEHARLS EIKNAHESLV ATKKILQDDK LALSTEKDPE IVAMLEEGVL VGEEAVERLS KQLENLLIPP DPDDDLSVIM ELRAGTGGDE AALFVGDCVR MYHLYAASKG WQCEVLSTSE SDLGGYKEYV MGISGASVKR FLQYEAGTHR VQRVPETETQ GRVHTSAVTV AVLPEPAEDD EEVFIDEKDL RIDTFRSSGA GGQHVNVTDS AVRITHIPSG VVVTCQDERS QHKNKAKAMR VLKARIRDAE VQKRAQEASA MRSAQVGSGD RSERIRTYNF PQNRVTDHRI GLTLYNLDRV MEGELDMITT ALVTHVHRQL FGHEETA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa taaaagtttt agagtgttta aagcgccttg aagaagttga aaagcagata tccgatccga atatctttag taatcctaaa gaatatagtt cgctgagcaa ggagcatgcg cgtctttctg agattaaaaa tgctcatgag tcattggttg cgacaaagaa aattcttcag gacgataaac tcgctttatc aacagagaag gatccagaaa tagtagctat gctagaagaa ggagttcttg taggggaaga ggctgtagaa cgtctatcga agcagttaga aaacctgctt attccacctg atccagatga tgatctcagt gtgattatgg agttgcgagc aggaacggga ggagatgaag cggctctttt tgtaggggac tgtgtgcgca tgtatcacct ttatgcagca agtaaggggt ggcaatgcga agttctctct acatcggagt cagatctcgg aggctacaaa gaatatgtta tggggatttc tggggcttct gtgaaacgtt tcttgcagta tgaagcagga acacatcgtg tgcaaagggt cccagaaaca gagactcagg gtagggtaca tacgtctgcg gtaacggtag ctgttcttcc agaaccagca gaagatgacg aagaagtttt cattgatgag aaggatttac gtattgatac cttccgttct tctggagccg gaggccagca cgtcaacgtt acagattccg ctgtgcgtat tactcatatt ccttctggcg ttgtcgttac gtgccaagat gaacgcagtc agcataaaaa taaagctaag gctatgcgcg tgctaaaagc tcgtattcgc gatgcagaag tgcagaagcg cgcgcaagaa gcctctgcta tgcgttctgc tcaggtagga agcggagatc gttcggagcg aattcgaacc tataattttc ctcaaaaccg tgtgaccgat caccgaattg gcttaacttt atataactta gatcgtgtaa tggaaggaga gttggatatg attacgacag ctcttgtaac ccacgtacat cggcagctat tcggtcatga agaaactgct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for ChtrB.17901.a.B1.PW38525

PURIFICATION DATe:	9/18/2018
CONCENTRATION:	13.48mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	190µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHME IKVLECLKRL EEVEKQISDP NIFSNPKEYS SLSKEHARLS EIKNAHESLV ATKKILQDDK LALSTEKDPE IVAMLEEGVL VGEEAVERLS KQLENLLIPP DPDDDLSVIM ELRAGTGGDE AALFVGDCVR MYHLYAASKG WQCEVLSTSE SDLGGYKEYV MGISGASVKR FLQYEAGTHR VQRVPETETQ GRVHTSAVTV AVLPEPAEDD EEVFIDEKDL RIDTFRSSGA GGQHVNVTDS AVRITHIPSG VVVTCQDERS QHKNKAKAMR VLKARIRDAE VQKRAQEASA MRSAQVGSGD RSERIRTYNF PQNRVTDHRI GLTLYNLDRV MEGELDMITT ALVTHVHRQL FGHEETA

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA TAAAAGTTTT AGAGTGTTTA AAGCGCCTTG AAGAAGTTGA AAAGCAGATA TCCGATCCGA ATATCTTTAG TAATCCTAAA GAATATAGTT CGCTGAGCAA GGAGCATGCG CGTCTTTCTG AGATTAAAAA TGCTCATGAG TCATTGGTTG CGACAAAGAA AATTCTTCAG GACGATAAAC TCGCTTTATC AACAGAGAAG GATCCAGAAA TAGTAGCTAT GCTAGAAGAA GGAGTTCTTG TAGGGGAAGA GGCTGTAGAA CGTCTATCGA AGCAGTTAGA AAACCTGCTT ATTCCACCTG ATCCAGATGA TGATCTCAGT GTGATTATGG AGTTGCGAGC AGGAACGGGA GGAGATGAAG CGGCTCTTTT TGTAGGGGAC TGTGTGCGCA TGTATCACCT TTATGCAGCA AGTAAGGGGT GGCAATGCGA AGTTCTCTCT ACATCGGAGT CAGATCTCGG AGGCTACAAA GAATATGTTA TGGGGATTTC TGGGGCTTCT GTGAAACGTT TCTTGCAGTA TGAAGCAGGA ACACATCGTG TGCAAAGGGT CCCAGAAACA GAGACTCAGG GTAGGGTACA TACGTCTGCG GTAACGGTAG CTGTTCTTCC AGAACCAGCA GAAGATGACG AAGAAGTTTT CATTGATGAG AAGGATTTAC GTATTGATAC CTTCCGTTCT TCTGGAGCCG GAGGCCAGCA CGTCAACGTT ACAGATTCCG CTGTGCGTAT TACTCATATT CCTTCTGGCG TTGTCGTTAC GTGCCAAGAT GAACGCAGTC AGCATAAAAA TAAAGCTAAG GCTATGCGCG TGCTAAAAGC TCGTATTCGC GATGCAGAAG TGCAGAAGCG CGCGCAAGAA GCCTCTGCTA TGCGTTCTGC TCAGGTAGGA AGCGGAGATC GTTCGGAGCG AATTCGAACC TATAATTTTC CTCAAAACCG TGTGACCGAT CACCGAATTG GCTTAACTTT ATATAACTTA GATCGTGTAA TGGAAGGAGA GTTGGATATG ATTACGACAG CTCTTGTAAC CCACGTACAT CGGCAGCTAT TCGGTCATGA AGAAACTGCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT