SSGCID: Target Page

ChtrB.17194.a

Uncharacterized protein CT_147

CENTER ID:	ChtrB.17194.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17194.a.B3.GE42251	soluble domain #2		900	1449

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.17194.a.B3.PW38519	soluble domain #2		900	1449

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272561.5.peg.160
UniProt:	O84149

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MANPSTPSFN HSDLSLQGRL RASSQQCTQA GQGDPQPLSP ESRGLTSNFS TRRDLIDVVE ESIETAKGSE LKKLRIYEIA LKILTIIGAA ILFAVPLCML LGVPLWIPIV TCIGVGIAFS IAKGCLQKRC QQIREEYRAL HLYHRYLLSN KDSIDGTLLS RFDIRFRKAE EKLHGLDLDK REANHPLEAD KRYDFAGLAH QRYQVDAALG ISSSQDAFWR GVAQQVKSVK DDVVLGDKAS TDLYPIAQQA LQAAGVGFSG AAGKESLLDL AKSLSSLFAW GSQVGKDSHE ALQQYQMRFL SSPILATWCG AGFSASAQDF VLKGENILDI ASENHTKMQN AIKRVQLVSV LGKMRNWKEK IDTLIQNKNL DQDSLRKLYQ DIEKAMHKVC IEDGVSTSIQ TQVRKVTQKY LRQDLQELLN KKAPLNESDL SKMQKGISSC ANLVVTLLES QLGTSGQTPI KEVEESIYRD LIATILQMGS AAGGVTPLVD GVHKAIREGK ALRSELSRAM SLHPRQSFLG VQSAVEKLQA FIRDPKWGAS AVHTSAEETL AQKQKFVSDL TRIQTSLADW RERYGLFEET KLNHIVSTDF VSRTEAFLDT LKNVAEACSL EQAVAELKDC EDAMKADLTH VEQKMNPTEI ESAREEFKRL MEELAGIQEQ LEQIAQPIYE EGVSGERLLL NTVFFHPEVL RKKVQAKEAS LEALTKGEQP SPTKKKTLKQ LSEGCEYFSS LVSKINALKT ILEGSRGKKI ASQDIRQLIG LTDELALELS SFQQDSLESL LYGLEGLSIP AASIEQKKGS PKSSSIAEKV VYASHQRVHN GVKAKVNRTL EAFSQLIKGL RGSLRNAMIT KAVVAAVLSV AFSCLAIALF SVQLTWLPIM LCVLALVLEA IPSALSIWVE KRNWKYEVAS LAKQLVSDGR KLPYPDLGDQ NIKHLEKIRD VYGLDGVAEL RVAEAALLGV QKLPEEQKQE SLKSAVKALR ADAKVLNKKF KKLPESYQPQ HSEVTGVQGV TEQESRDDVL VAQDMAAIEE LQDQYHAACL QFESVSTRFL AEQRKAKFLE ELLVQKRRDV SHLSHQEAHY TQVVSHLKEL ISMRKGASTQ HASKEEISTK MRELLSLDDQ LLKAHTAQDV NRDNSINGQL QQQFKKLSEE GSLQKVKALL ELNMCLGNAG QTLYHSRLKR EVFEASLSGT SRQLLQYGED LFASYDGSDR SALLRFVLGS GYEMISEASS ELKSLRKRWK RSASQAAIAP EDYEKVCRVL ERFLKARDSL RPKLGLPLGK SSDATVGLQH QIRDNQRVKA RVTACYQESC RNVLQHLEDW VRKTRQESAE CQKVETKIRE FCQKAGSKEN LAESTEMLFS SLEEDLNKIP LDVLRAILRS LSSKVLHIRD QKLELEKLEE QFAKTNAIVK AKEAEFEKNG EVWHNQYQML KSQMEKLESQ KRRLTDKKE

NT Sequence

atggcgaatc cgtctacacc ctcattcaat cattccgacc tttctttaca aggtcgttta agagcttcaa gtcagcaatg tacgcaggct ggacaagggg accctcaacc tttgagtcca gagtccagag gcttgacctc aaacttttct actcggcgag atttaattga tgttgtagag gagtctatag agactgctaa gggcagcgag cttaaaaaac ttcgaatata tgagattgct ctaaagattc ttacaattat tggagccgcg attctcttcg ctgttcctct ttgtatgttg ctcggtgtac ctttatggat tcctattgta acgtgtatcg gtgtaggaat tgcttttagt atcgccaaag gatgcttaca gaaaagatgt cagcagattc gagaagaata tcgtgctcta catctctatc atcgctatct actttccaac aaagattcca ttgatgggac tcttttgagt cgcttcgata tccgttttcg aaaagcggaa gagaaattac acgggttaga tcttgataaa agagaggcta atcatccact agaagcggac aagagatatg attttgccgg gttggctcat caacgctacc aggtggatgc agctcttgga atctctagta gccaagacgc tttttggaga ggggttgctc agcaggtaaa atctgttaag gacgatgttg ttttagggga taaggcgagt acagatctgt acccgatagc gcaacaggct ctacaagcag cgggggtagg tttctctggc gctgcaggga aagagtcttt gttggatcta gcaaaatctt tatccagtct gtttgcgtgg ggttctcaag tcggcaaaga ctctcacgaa gctttacagc aatatcaaat gcgcttttta agtagtccca tcttagctac gtggtgtggg gctgggtttt ccgcatctgc tcaggatttt gttcttaaag gtgagaatat cttagatatt gctagtgaaa atcatacgaa gatgcagaat gctatcaaac gtgtgcagct agtttccgtt ttaggcaaaa tgagaaattg gaaagagaag attgataccc taatccaaaa caaaaatctt gatcaagact ctctacgaaa actgtaccaa gacattgaaa aagctatgca taaggtttgt atcgaagatg gggtttccac ttctatacag actcaggtgc gtaaggtcac acaaaaatat ttacgacaag atttacaaga gcttcttaat aagaaagcac cattaaatga aagcgatctt tctaaaatgc aaaaaggcat tagttcgtgt gctaatcttg ttgtcacact cttagaaagc cagttaggaa cttcggggca gactcctata aaagaagtcg aagagagtat ttaccgagac ttgatcgcta ctattttaca aatgggaagt gcggcaggag gagtgacacc attagttgat ggtgtacata aagctattag agaaggaaaa gctttacgta gcgaacttag ccgggctatg tctttacatc caagacaatc tttcctaggg gtgcaatctg ctgtagagaa gttgcaagca tttatccgag atcctaagtg gggagcatcg gcagtgcata cctctgctga agagactcta gcgcaaaaac agaagtttgt ttctgatctt acgcgcatcc aaacgagcct agcagactgg agagaacgtt acgggctatt tgaagagaca aaactgaatc atattgtgtc tacggacttt gtatcgagaa cagaagcttt tctagatacc ctgaaaaacg ttgctgaagc atgttctctg gagcaagctg ttgcagagct caaagattgt gaggatgcta tgaaagcaga tctcactcat gttgagcaaa aaatgaatcc tacagagata gagtctgcaa gagaagagtt taagcggttg atggaagagc tagctggtat tcaagagcag ctagaacaga tcgctcaacc tatttatgaa gaaggggtaa gcggtgaacg tcttctactt aatacagtct tctttcatcc agaagtatta cgtaagaaag ttcaagcaaa agaagcctcg ttagaggctt taacaaaagg cgaacagcct tctccaacga agaagaaaac gttgaagcag ctttctgaag gatgtgagta cttctctagt cttgtaagca agattaatgc gcttaagaca atattagaag gttctagagg caagaaaatt gcgtcccaag atatacgaca gctgattgga ttgactgatg agcttgctct agagttgtcc tctttccaac aggattcttt agagagtttg ctctatggat tagaggggtt aagcattcca gctgcttcta tagaacagaa gaaaggatct cctaagtctt cttctatagc agagaaggtg gtgtatgctt ctcatcagcg tgtccataat ggggtaaaag cgaaagtgaa tcgcacatta gaagcatttt cacagctgat caaaggctta cgaggatctt tacgtaatgc gatgatcact aaagctgttg tagcggcggt tctctctgta gctttttcct gcctagcgat tgcgctcttc tctgtgcagc ttacatggct tcctattatg ctctgcgttt tagctttggt attggaagct atcccttctg ctttatctat ttgggtggag aaaagaaact ggaaatatga ggttgcctct ttagcgaagc agttagtttc ggatggaaga aagcttcctt atccagattt gggggatcaa aatatcaagc atctagagaa gattcgagat gtttatgggc tggatggtgt tgcagaatta cgggtagctg aagcagcttt attaggagtt cagaaacttc ctgaagagca aaaacaagaa tctttaaaaa gtgctgttaa agcattgcgg gcggatgcga aggttcttaa taagaaattt aagaagcttc ctgagtcata tcagcctcaa cactctgaag tcacaggagt ccaaggtgta acggaacaag aaagcaggga tgacgttttg gtagcacaag atatggctgc catagaagaa ttgcaagacc agtatcatgc agcttgcttg caatttgagt ctgtgagtac gcgattttta gccgaacagc gtaaagctaa gtttctggaa gagctgttag ttcaaaaacg tcgagatgtg tcccatttat ctcatcaaga agctcattat actcaagtag tcagtcattt gaaagagctc atctcaatga gaaagggagc atctactcaa cacgcttcta aagaagagat ttctacaaaa atgagagagc tgttgtcttt agatgatcaa cttctaaaag ctcatacagc tcaagatgtg aaccgggata atagcataaa cggtcaactg caacagcagt ttaaaaagtt atctgaagaa ggaagcctac aaaaagtaaa agctctgcta gaactaaata tgtgtttagg taatgctggg caaacccttt atcattcaag gctaaagaga gaggttttcg aagcatctct ctctggaacc tctcggcaac ttcttcaata cggtgaagat ctgtttgcat cttacgatgg aagtgatcga tcggctcttc tacggtttgt tttaggatcc ggatatgaga tgatcagtga ggccagctct gagctgaagt ctctacgcaa acgttggaaa agaagcgctt ctcaagccgc aattgctcct gaagattatg agaaagtctg cagagtgtta gaacgttttc ttaaagcgcg agacagtctg cgtccgaagt taggcttacc tcttggtaag agctcagatg ctaccgttgg tttacaacat caaatacgag ataatcaacg agttaaagct cgagtaaccg cttgttacca agagagttgc agaaatgttt tacagcattt agaagattgg gtgcggaaaa cgcgacagga gtcggcagaa tgtcaaaaag tagaaacaaa aatacgcgag ttctgccaaa aagccggatc taaggagaat cttgctgaat ctacagagat gctattttct agcttagaag aagatttgaa taaaatacct ctagatgttt tgcgtgctat tttacgatct ttgtcttcta aagttcttca tattagggat caaaagttag aacttgaaaa gttagaagag cagtttgcga agacaaatgc tattgtaaaa gccaaggaag ctgagttcga gaagaatggg gaagtgtggc ataatcagta tcagatgcta aaaagtcaga tggagaagct ggagtctcag aaaagaagac tgacagataa gaaagaataa

Details for ChtrB.17194.a.B3.GE42251

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	76
PERCENT COVERAGE:	58

Validated AA Sequence

MAHHHHHHME KRNWKYEVAS LAKQLVSDGR KLPYPDLGDQ NIKHLEKIRD VYGLDGVAEL RVAEAALLGV QKLPEEQKQE SLKSAVKALR ADAKVLNKKF KKLPESYQPQ HSEVTGVQGV TEQESRDDVL VAQDMAAIEE LQDQYHAACL QFESVSMRFL AEQRKAKFLE KLLIQKRRDV SHLSHQEAHY TQVVSHLKER ISMRKGASTQ HASKEEISTK MRELLSLDDX XLKAHTAQDV NWDNSINXXX XXXXXKXXXX XXXXKVKALL XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX ICLHLTXXXX DXXFYGLX

Validated NT Sequence

tcttttctga gactccagct tctccatctg actttttagc atctgatact gattatgcca cacttcccca ttcttctcga actcagcttc cttggctttt acaataatat ttgtcttcgc aaactgctct tctaactttt caagttctaa cttttgatcc ctaatatgaa gaactttaga agataaagat cgtaaaatag cacgcaaaac atctagaggt attttattca aatcttcttc taagctagaa aatagcatct ctgtagattc agcaagattc tccttagatc cggctttttg gcagaactcg cgtatttttg tttctacttt ttgacattct gccgactcct gtcgcgtttt ccgcacccaa tcttctaaat gctgtaaaac atttctgcaa ctctcttggt aacaagcggt tactcgagct ttaactcgtt gattatctcg tatttgatgt tgtaaaccaa cggtagcatc tgagctctta ccaagaggta agcctaactt cggacgcaga ctgtctcgcg ctttaagaaa acgttctaac actctgcaga ctttctcata atcttcagga gcaattgcgg cttgagaagc gcttcttttc caacgtttgc gtagagactt cagctcagag ctggcctcac tgatcatctc atatccagat cctannncaa accgtagaag nnnnnatcga nnnnnnnnnn cgtaagatgc aaacagatct nnnnnnnnnn nnnnnnnttn cnnnnnnnnn nnnnnnnnnn nnnnntnnnn annnnnnnnn nnnnnnnnnn nnnnnnnnnn nggnnnnncn nnnnnnnnnn nnannnnnnn ntnannnnta gcagngcttt tactttttnn nnnnnnnnnn cnnnagnnna cttttnnnnn nnnnnnnnnn nnnnnncgtt tatgctatta tcccagttca catcttgagc tgtatgagct tttagannnt gatcatctaa agacaacagc tctctcattt tngtagaaat ctcttcttta gaagcgtgtt gagtagatgc tccttttctc attgagatgc gctctttcaa atgactgact acttgagtat aatgagcttc ttgatgagat aaatgggaca catctcgacg tttttgaatt aacagctttt ccagaaactt agctttacgc tgttcggcta aaaatcgcat gctcacagac tcaaattgca agcaagctgc atgatactgg tcttgcaact cttctatggc agccatatct tgtgctacca aaacgtcatc cctgctttct tgttccgtta caccttggac tcctgtgact tcagagtgtt gaggctgata tgactcagga agcttcttaa atttcttatt aagaaccttc gcatccgccc gcaatgcttt aacagcactt tttaaagatt cttgtttttg ctcttcagga agtttctgaa ctcctaataa agctgcttca gctacccgta attctgcaac accatccagc ccataaacat ctcgaatctt ctctagatgc ttgatatttt gatcccccaa atctggataa ggaagctttc ttccatccga aaccaactgc ttcgctaaag aggcaacctc atatttccag tttcttttct ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHEK RNWKYEVASL AKQLVSDGRK LPYPDLGDQN IKHLEKIRDV YGLDGVAELR VAEAALLGVQ KLPEEQKQES LKSAVKALRA DAKVLNKKFK KLPESYQPQH SEVTGVQGVT EQESRDDVLV AQDMAAIEEL QDQYHAACLQ FESVSTRFLA EQRKAKFLEE LLVQKRRDVS HLSHQEAHYT QVVSHLKELI SMRKGASTQH ASKEEISTKM RELLSLDDQL LKAHTAQDVN RDNSINGQLQ QQFKKLSEEG SLQKVKALLE LNMCLGNAGQ TLYHSRLKRE VFEASLSGTS RQLLQYGEDL FASYDGSDRS ALLRFVLGSG YEMISEASSE LKSLRKRWKR SASQAAIAPE DYEKVCRVLE RFLKARDSLR PKLGLPLGKS SDATVGLQHQ IRDNQRVKAR VTACYQESCR NVLQHLEDWV RKTRQESAEC QKVETKIREF CQKAGSKENL AESTEMLFSS LEEDLNKIPL DVLRAILRSL SSKVLHIRDQ KLELEKLEEQ FAKTNAIVKA KEAEFEKNGE VWHNQYQMLK SQMEKLESQK RRLTDKKE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagaaaa gaaactggaa atatgaggtt gcctctttag cgaagcagtt agtttcggat ggaagaaagc ttccttatcc agatttgggg gatcaaaata tcaagcatct agagaagatt cgagatgttt atgggctgga tggtgttgca gaattacggg tagctgaagc agctttatta ggagttcaga aacttcctga agagcaaaaa caagaatctt taaaaagtgc tgttaaagca ttgcgggcgg atgcgaaggt tcttaataag aaatttaaga agcttcctga gtcatatcag cctcaacact ctgaagtcac aggagtccaa ggtgtaacgg aacaagaaag cagggatgac gttttggtag cacaagatat ggctgccata gaagaattgc aagaccagta tcatgcagct tgcttgcaat ttgagtctgt gagtacgcga tttttagccg aacagcgtaa agctaagttt ctggaagagc tgttagttca aaaacgtcga gatgtgtccc atttatctca tcaagaagct cattatactc aagtagtcag tcatttgaaa gagctcatct caatgagaaa gggagcatct actcaacacg cttctaaaga agagatttct acaaaaatga gagagctgtt gtctttagat gatcaacttc taaaagctca tacagctcaa gatgtgaacc gggataatag cataaacggt caactgcaac agcagtttaa aaagttatct gaagaaggaa gcctacaaaa agtaaaagct ctgctagaac taaatatgtg tttaggtaat gctgggcaaa ccctttatca ttcaaggcta aagagagagg ttttcgaagc atctctctct ggaacctctc ggcaacttct tcaatacggt gaagatctgt ttgcatctta cgatggaagt gatcgatcgg ctcttctacg gtttgtttta ggatccggat atgagatgat cagtgaggcc agctctgagc tgaagtctct acgcaaacgt tggaaaagaa gcgcttctca agccgcaatt gctcctgaag attatgagaa agtctgcaga gtgttagaac gttttcttaa agcgcgagac agtctgcgtc cgaagttagg cttacctctt ggtaagagct cagatgctac cgttggttta caacatcaaa tacgagataa tcaacgagtt aaagctcgag taaccgcttg ttaccaagag agttgcagaa atgttttaca gcatttagaa gattgggtgc ggaaaacgcg acaggagtcg gcagaatgtc aaaaagtaga aacaaaaata cgcgagttct gccaaaaagc cggatctaag gagaatcttg ctgaatctac agagatgcta ttttctagct tagaagaaga tttgaataaa atacctctag atgttttgcg tgctatttta cgatctttgt cttctaaagt tcttcatatt agggatcaaa agttagaact tgaaaagtta gaagagcagt ttgcgaagac aaatgctatt gtaaaagcca aggaagctga gttcgagaag aatggggaag tgtggcataa tcagtatcag atgctaaaaa gtcagatgga gaagctggag tctcagaaaa gaagactgac agataagaaa gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for ChtrB.17194.a.B3.PW38519

PURIFICATION DATe:	8/7/2018
CONCENTRATION:	10mg/ml
OBSERVED MW:	63kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25mM HEPES, 500mM NaCl, 5% glycerol 0.025% azide, 2mM DTT
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	76
PERCENT COVERAGE:	58

Protocol Notes

Insoluble protein for antibody production

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAGAAAA GAAACTGGAA ATATGAGGTT GCCTCTTTAG CGAAGCAGTT AGTTTCGGAT GGAAGAAAGC TTCCTTATCC AGATTTGGGG GATCAAAATA TCAAGCATCT AGAGAAGATT CGAGATGTTT ATGGGCTGGA TGGTGTTGCA GAATTACGGG TAGCTGAAGC AGCTTTATTA GGAGTTCAGA AACTTCCTGA AGAGCAAAAA CAAGAATCTT TAAAAAGTGC TGTTAAAGCA TTGCGGGCGG ATGCGAAGGT TCTTAATAAG AAATTTAAGA AGCTTCCTGA GTCATATCAG CCTCAACACT CTGAAGTCAC AGGAGTCCAA GGTGTAACGG AACAAGAAAG CAGGGATGAC GTTTTGGTAG CACAAGATAT GGCTGCCATA GAAGAATTGC AAGACCAGTA TCATGCAGCT TGCTTGCAAT TTGAGTCTGT GAGTACGCGA TTTTTAGCCG AACAGCGTAA AGCTAAGTTT CTGGAAGAGC TGTTAGTTCA AAAACGTCGA GATGTGTCCC ATTTATCTCA TCAAGAAGCT CATTATACTC AAGTAGTCAG TCATTTGAAA GAGCTCATCT CAATGAGAAA GGGAGCATCT ACTCAACACG CTTCTAAAGA AGAGATTTCT ACAAAAATGA GAGAGCTGTT GTCTTTAGAT GATCAACTTC TAAAAGCTCA TACAGCTCAA GATGTGAACC GGGATAATAG CATAAACGGT CAACTGCAAC AGCAGTTTAA AAAGTTATCT GAAGAAGGAA GCCTACAAAA AGTAAAAGCT CTGCTAGAAC TAAATATGTG TTTAGGTAAT GCTGGGCAAA CCCTTTATCA TTCAAGGCTA AAGAGAGAGG TTTTCGAAGC ATCTCTCTCT GGAACCTCTC GGCAACTTCT TCAATACGGT GAAGATCTGT TTGCATCTTA CGATGGAAGT GATCGATCGG CTCTTCTACG GTTTGTTTTA GGATCCGGAT ATGAGATGAT CAGTGAGGCC AGCTCTGAGC TGAAGTCTCT ACGCAAACGT TGGAAAAGAA GCGCTTCTCA AGCCGCAATT GCTCCTGAAG ATTATGAGAA AGTCTGCAGA GTGTTAGAAC GTTTTCTTAA AGCGCGAGAC AGTCTGCGTC CGAAGTTAGG CTTACCTCTT GGTAAGAGCT CAGATGCTAC CGTTGGTTTA CAACATCAAA TACGAGATAA TCAACGAGTT AAAGCTCGAG TAACCGCTTG TTACCAAGAG AGTTGCAGAA ATGTTTTACA GCATTTAGAA GATTGGGTGC GGAAAACGCG ACAGGAGTCG GCAGAATGTC AAAAAGTAGA AACAAAAATA CGCGAGTTCT GCCAAAAAGC CGGATCTAAG GAGAATCTTG CTGAATCTAC AGAGATGCTA TTTTCTAGCT TAGAAGAAGA TTTGAATAAA ATACCTCTAG ATGTTTTGCG TGCTATTTTA CGATCTTTGT CTTCTAAAGT TCTTCATATT AGGGATCAAA AGTTAGAACT TGAAAAGTTA GAAGAGCAGT TTGCGAAGAC AAATGCTATT GTAAAAGCCA AGGAAGCTGA GTTCGAGAAG AATGGGGAAG TGTGGCATAA TCAGTATCAG ATGCTAAAAA GTCAGATGGA GAAGCTGGAG TCTCAGAAAA GAAGACTGAC AGATAAGAAA GAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT