ChtrB.17127.a
Glucose-6-phosphate isomerase (GPI) (EC 5.3.1.9) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI)
| CENTER ID: | ChtrB.17127.a |
| ORGANISM: | Chlamydia trachomatis D/UW-3/Cx |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.17127.a.B1.GE42414 | full length | 1 | 525 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.17127.a.B1.PW38526 | full length | 1 | 525 |
Structures
6OTU
DEPOSITED: 5/3/2019
DETERMINATION: XRay
CLONE: ChtrB.17127.a.B1.GE42414
PROTEIN: ChtrB.17127.a.B1.PW38526
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_82022 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272561.5.peg.407 |
| UniProt: | P0CD72 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMGKGFLDCE SLVALQEMAL HPIDLTASGC LSEERIQKNS LSAEGFTYSY ATERVDDRCL EALQGLTEER ELIKQMECMQ QGAIMNRIEG FQSESRPVLH TATRAWVRDQ DLHEEAAAIA RHSKEEALRL AEFLYIARAK FSTLVQMGIG GSELGPKAMY FAMQGSCPSD KRIFFVSNID PDNAAEVLRE IDLEQTLVVV VSKSGTTLEP AANEELFRQA YQNKGLSIAE HFVAVTSQGS PMDDKSRYLE VFHLWDSIGG RFSATSMVGG VVLGFAFGYE AFIEFLQGAA AIDAHALTPK MRENLPLLSA MLGVWNRNLL GYPTTAVIPY STGLKYFTAH LQQCGMESNG KSISREGKEI SFRTSPIIWG DVGTNCQHSF FQSLHQGTDI VPVEFIGFLH NQRGLDCVLS GSSSSQKLFA NLVAQSLALA QGRDNANSNK RFKGNRPSSI LVAQQLSPRI AGSLLAFYEH KFAFQGFCWG INSFDQEGVS LGKELATQII GIMSGNAPVE FSEARGMLRL FNVLT
NT Sequence
atgatgggta aaggtttttt agattgtgag tcattagttg ctttgcaaga gatggctttg catccgattg atttgactgc atcaggatgc ttatctgaag agcgtattca gaaaaactct ttatctgcag aaggatttac ctatagttat gctacggaac gtgtagatga tcgctgctta gaggctttac aaggacttac tgaagagagg gaattgatca aacagatgga gtgtatgcaa caaggcgcca tcatgaatcg tatagaaggt tttcagagcg aatctcgccc tgtattgcat acggcaacac gcgcttgggt tcgagatcaa gatcttcatg aagaagctgc tgcgatagca agacactcta aggaggaagc tcttcgtctt gcagagtttc tgtatatagc tcgtgctaaa ttttctacgc ttgtacagat gggtattggg ggctcggagt tgggccctaa agctatgtat tttgctatgc aaggctcttg cccatctgat aagagaatct tttttgtctc caatattgat ccggataatg ctgcagaagt attacgggag atagatttgg agcaaacact ggttgttgtg gtttctaagt caggaacaac tttagagcct gcagcaaatg aagagctgtt cagacaggct tatcaaaata aaggtttatc tattgcagag cattttgtag cggtaacttc tcaaggcagc cctatggatg ataagagtcg ttatctagaa gtattccatc tttgggatag tattggcgga agattttcag ctacatccat ggtaggagga gttgtattag gttttgcttt cggttacgaa gcttttatag agtttttgca aggagcagct gctatcgatg ctcatgcgct gactccaaaa atgagagaga atcttcctct tctatctgcg atgcttggag tttggaatcg taatctgtta gggtatccta caacagcagt gattccttat tcgacaggat tgaaatattt tacagcgcat ttacaacagt gtggaatgga atccaatgga aaaagtattt cgagagaagg gaaggagatt agttttagga cttcacccat tatttggggc gatgtgggga caaactgtca gcattctttt ttccaaagtc ttcatcaagg aacagatatt gttcccgtag agttcattgg atttttgcat aaccaacgag ggctagattg tgtattatca ggaagctcat catctcaaaa gctctttgca aaccttgttg ctcaatcttt agctttagct cagggtagag ataatgcgaa ctcgaataag aggtttaaag ggaacagacc ttcttcgata ttggtagcgc aacagctttc tcctcgtatt gctggtagtt tattggcttt ttatgagcat aaatttgcat ttcaagggtt ttgctgggga atcaattctt ttgaccaaga aggggtttca ttagggaaag aattggcaac gcaaattata ggcatcatgt cggggaatgc tcctgtagag ttttccgaag ctcgaggtat gttaagactt tttaacgttt taacataa
Details for ChtrB.17127.a.B1.GE42414
| HARVESTED ON: | 5/25/2018 |
| SEQUENCED ON: | 5/31/2018 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 59kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMM GKGFLDCESL VALQEMALHP IDLTASGCLS EERIQKNSLS VEGFTYSYAT ERVDDRCLEA LQGLTEEREL IKQMECMQQG AIMNRIEGFQ SESRPVLHTA TRAWVRDQDL HEEAAAIARH SKEEALRLAE FLYIARAKFS TLVQIGIGGS ELGPKAMYFA MQGSCPSDKR IFFVSNIDPD NAAEVLREID LEQTLVVVVS KSGTTLEPAA NEELFRQAYQ NKGLSIAEHF VAVTSQGSPM DDKSRYLEVF HLWDSIGGRF SATSMVGGVV LGFAFGYEAF IEFLQGAAAI DAHALTPKMR ENLPLLSAML GVWNRNLLGY PTTAVIPYST GLKYFTAHLQ QCGMESNGKS ISREGKEISF RTSPIIWGDV GTNCQHSFFQ SLHQGTDIVP VEFIGFLHNQ RGLDCVLSGS SSSQKLFANL VAQSLALAQG RDNANPNKRF KGNSPSSILV AQQLSPRIAG SLLAFYEHKF AFQGFCWGIN SFDQEGVSLG KELATQIIGI MSGNAPVEFP EARGV
Validated NT Sequence
taacacacct cgagcttcgg gaaactctac aggagcattc cccgacatga tgcctataat ttgcgttgcc aattctttcc ctaatgaaac cccttcttgg tcaaaagaat tgattcccca gcaaaaccct tgaaatgcaa atttatgctc ataaaaagcc aataaactac cagcaatacg aggagaaagc tgttgcgcta ccaatatcga agaaggactg ttccctttaa acctcttatt cgggttcgca ttatctctac cctgagctaa agctaaagat tgagcaacaa ggtttgcaaa gagcttttga gatgatgagc ttcctgataa tacacaatct agccctcgtt ggttatgcaa aaatccaatg aactctacgg gaacaatatc tgttccttga tgaagacttt ggaaaaaaga atgctgacag tttgtcccca catcgcccca aataatgggt gaagtcctaa aactaatctc cttcccttct ctcgaaatac tttttccatt ggattccatt ccacactgtt gtaaatgcgc tgtaaaatat ttcaatcctg tcgaataagg aatcactgct gttgtaggat accctaacag attacgattc caaactccaa gcatcgcaga tagaagagga agattctctc tcatttttgg agtcagcgca tgagcatcga tagcagctgc tccttgcaaa aactctataa aagcttcgta accgaaagca aaacctaata caactcctcc taccatggat gtagctgaaa atcttccgcc aatactatcc caaagatgga atacttctag ataacgactc ttatcatcca tagggctgcc ttgagaagtt accgctacaa aatgctctgc aatagataaa cctttatttt gataagcctg tctgaacagt tcttcatttg ctgcaggctc taaagttgtt cctgacttag aaaccacaac aaccagtgtt tgctccaaat ctatctcccg taatacttct gcagcattat ccggatcaat attggagaca aaaaagattc tcttatcaga tgggcaagag ccttgcatag caaaatacat agctttaggg cccaactccg agcccccaat acctatctgt acaagcgtag aaaatttagc acgagctata tacagaaact ctgcaagacg aagagcttcc tccttagagt gtcttgctat cgcagcagct tcttcatgaa gatcttgatc tcgaacccaa gcgcgtgttg ccgtatgcaa tacagggcga gattcgctct gaaaaccttc tatacgattc atgatggcgc cttgttgcat acactccatc tgtttgatca attccctctc ttcagtaagt ccttgtaaag cctctaagca gcgatcatct acacgttccg tagcataact ataggtaaat ccttctacag ataaagagtt tttctgaata cgctcttcag ataagcatcc tgatgcagtc aaatcaatcg gatgcaaagc catctcttgc aaagcaacta atgactcaca atctaaaaaa cctttaccca tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMM GKGFLDCESL VALQEMALHP IDLTASGCLS EERIQKNSLS AEGFTYSYAT ERVDDRCLEA LQGLTEEREL IKQMECMQQG AIMNRIEGFQ SESRPVLHTA TRAWVRDQDL HEEAAAIARH SKEEALRLAE FLYIARAKFS TLVQMGIGGS ELGPKAMYFA MQGSCPSDKR IFFVSNIDPD NAAEVLREID LEQTLVVVVS KSGTTLEPAA NEELFRQAYQ NKGLSIAEHF VAVTSQGSPM DDKSRYLEVF HLWDSIGGRF SATSMVGGVV LGFAFGYEAF IEFLQGAAAI DAHALTPKMR ENLPLLSAML GVWNRNLLGY PTTAVIPYST GLKYFTAHLQ QCGMESNGKS ISREGKEISF RTSPIIWGDV GTNCQHSFFQ SLHQGTDIVP VEFIGFLHNQ RGLDCVLSGS SSSQKLFANL VAQSLALAQG RDNANSNKRF KGNRPSSILV AQQLSPRIAG SLLAFYEHKF AFQGFCWGIN SFDQEGVSLG KELATQIIGI MSGNAPVEFS EARGMLRLFN VLT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgg gtaaaggttt tttagattgt gagtcattag ttgctttgca agagatggct ttgcatccga ttgatttgac tgcatcagga tgcttatctg aagagcgtat tcagaaaaac tctttatctg cagaaggatt tacctatagt tatgctacgg aacgtgtaga tgatcgctgc ttagaggctt tacaaggact tactgaagag agggaattga tcaaacagat ggagtgtatg caacaaggcg ccatcatgaa tcgtatagaa ggttttcaga gcgaatctcg ccctgtattg catacggcaa cacgcgcttg ggttcgagat caagatcttc atgaagaagc tgctgcgata gcaagacact ctaaggagga agctcttcgt cttgcagagt ttctgtatat agctcgtgct aaattttcta cgcttgtaca gatgggtatt gggggctcgg agttgggccc taaagctatg tattttgcta tgcaaggctc ttgcccatct gataagagaa tcttttttgt ctccaatatt gatccggata atgctgcaga agtattacgg gagatagatt tggagcaaac actggttgtt gtggtttcta agtcaggaac aactttagag cctgcagcaa atgaagagct gttcagacag gcttatcaaa ataaaggttt atctattgca gagcattttg tagcggtaac ttctcaaggc agccctatgg atgataagag tcgttatcta gaagtattcc atctttggga tagtattggc ggaagatttt cagctacatc catggtagga ggagttgtat taggttttgc tttcggttac gaagctttta tagagttttt gcaaggagca gctgctatcg atgctcatgc gctgactcca aaaatgagag agaatcttcc tcttctatct gcgatgcttg gagtttggaa tcgtaatctg ttagggtatc ctacaacagc agtgattcct tattcgacag gattgaaata ttttacagcg catttacaac agtgtggaat ggaatccaat ggaaaaagta tttcgagaga agggaaggag attagtttta ggacttcacc cattatttgg ggcgatgtgg ggacaaactg tcagcattct tttttccaaa gtcttcatca aggaacagat attgttcccg tagagttcat tggatttttg cataaccaac gagggctaga ttgtgtatta tcaggaagct catcatctca aaagctcttt gcaaaccttg ttgctcaatc tttagcttta gctcagggta gagataatgc gaactcgaat aagaggttta aagggaacag accttcttcg atattggtag cgcaacagct ttctcctcgt attgctggta gtttattggc tttttatgag cataaatttg catttcaagg gttttgctgg ggaatcaatt cttttgacca agaaggggtt tcattaggga aagaattggc aacgcaaatt ataggcatca tgtcggggaa tgctcctgta gagttttccg aagctcgagg tatgttaaga ctttttaacg ttttaacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for ChtrB.17127.a.B1.PW38526
| PURIFICATION DATe: | 9/18/2018 |
| CONCENTRATION: | 18.94mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 41 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMM GKGFLDCESL VALQEMALHP IDLTASGCLS EERIQKNSLS VEGFTYSYAT ERVDDRCLEA LQGLTEEREL IKQMECMQQG AIMNRIEGFQ SESRPVLHTA TRAWVRDQDL HEEAAAIARH SKEEALRLAE FLYIARAKFS TLVQIGIGGS ELGPKAMYFA MQGSCPSDKR IFFVSNIDPD NAAEVLREID LEQTLVVVVS KSGTTLEPAA NEELFRQAYQ NKGLSIAEHF VAVTSQGSPM DDKSRYLEVF HLWDSIGGRF SATSMVGGVV LGFAFGYEAF IEFLQGAAAI DAHALTPKMR ENLPLLSAML GVWNRNLLGY PTTAVIPYST GLKYFTAHLQ QCGMESNGKS ISREGKEISF RTSPIIWGDV GTNCQHSFFQ SLHQGTDIVP VEFIGFLHNQ RGLDCVLSGS SSSQKLFANL VAQSLALAQG RDNANPNKRF KGNSPSSILV AQQLSPRIAG SLLAFYEHKF AFQGFCWGIN SFDQEGVSLG KELATQIIGI MSGNAPVEFP EARGV
Validated NT Sequence
taacacacct cgagcttcgg gaaactctac aggagcattc cccgacatga tgcctataat ttgcgttgcc aattctttcc ctaatgaaac cccttcttgg tcaaaagaat tgattcccca gcaaaaccct tgaaatgcaa atttatgctc ataaaaagcc aataaactac cagcaatacg aggagaaagc tgttgcgcta ccaatatcga agaaggactg ttccctttaa acctcttatt cgggttcgca ttatctctac cctgagctaa agctaaagat tgagcaacaa ggtttgcaaa gagcttttga gatgatgagc ttcctgataa tacacaatct agccctcgtt ggttatgcaa aaatccaatg aactctacgg gaacaatatc tgttccttga tgaagacttt ggaaaaaaga atgctgacag tttgtcccca catcgcccca aataatgggt gaagtcctaa aactaatctc cttcccttct ctcgaaatac tttttccatt ggattccatt ccacactgtt gtaaatgcgc tgtaaaatat ttcaatcctg tcgaataagg aatcactgct gttgtaggat accctaacag attacgattc caaactccaa gcatcgcaga tagaagagga agattctctc tcatttttgg agtcagcgca tgagcatcga tagcagctgc tccttgcaaa aactctataa aagcttcgta accgaaagca aaacctaata caactcctcc taccatggat gtagctgaaa atcttccgcc aatactatcc caaagatgga atacttctag ataacgactc ttatcatcca tagggctgcc ttgagaagtt accgctacaa aatgctctgc aatagataaa cctttatttt gataagcctg tctgaacagt tcttcatttg ctgcaggctc taaagttgtt cctgacttag aaaccacaac aaccagtgtt tgctccaaat ctatctcccg taatacttct gcagcattat ccggatcaat attggagaca aaaaagattc tcttatcaga tgggcaagag ccttgcatag caaaatacat agctttaggg cccaactccg agcccccaat acctatctgt acaagcgtag aaaatttagc acgagctata tacagaaact ctgcaagacg aagagcttcc tccttagagt gtcttgctat cgcagcagct tcttcatgaa gatcttgatc tcgaacccaa gcgcgtgttg ccgtatgcaa tacagggcga gattcgctct gaaaaccttc tatacgattc atgatggcgc cttgttgcat acactccatc tgtttgatca attccctctc ttcagtaagt ccttgtaaag cctctaagca gcgatcatct acacgttccg tagcataact ataggtaaat ccttctacag ataaagagtt tttctgaata cgctcttcag ataagcatcc tgatgcagtc aaatcaatcg gatgcaaagc catctcttgc aaagcaacta atgactcaca atctaaaaaa cctttaccca tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMM GKGFLDCESL VALQEMALHP IDLTASGCLS EERIQKNSLS AEGFTYSYAT ERVDDRCLEA LQGLTEEREL IKQMECMQQG AIMNRIEGFQ SESRPVLHTA TRAWVRDQDL HEEAAAIARH SKEEALRLAE FLYIARAKFS TLVQMGIGGS ELGPKAMYFA MQGSCPSDKR IFFVSNIDPD NAAEVLREID LEQTLVVVVS KSGTTLEPAA NEELFRQAYQ NKGLSIAEHF VAVTSQGSPM DDKSRYLEVF HLWDSIGGRF SATSMVGGVV LGFAFGYEAF IEFLQGAAAI DAHALTPKMR ENLPLLSAML GVWNRNLLGY PTTAVIPYST GLKYFTAHLQ QCGMESNGKS ISREGKEISF RTSPIIWGDV GTNCQHSFFQ SLHQGTDIVP VEFIGFLHNQ RGLDCVLSGS SSSQKLFANL VAQSLALAQG RDNANSNKRF KGNRPSSILV AQQLSPRIAG SLLAFYEHKF AFQGFCWGIN SFDQEGVSLG KELATQIIGI MSGNAPVEFS EARGMLRLFN VLT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATGG GTAAAGGTTT TTTAGATTGT GAGTCATTAG TTGCTTTGCA AGAGATGGCT TTGCATCCGA TTGATTTGAC TGCATCAGGA TGCTTATCTG AAGAGCGTAT TCAGAAAAAC TCTTTATCTG CAGAAGGATT TACCTATAGT TATGCTACGG AACGTGTAGA TGATCGCTGC TTAGAGGCTT TACAAGGACT TACTGAAGAG AGGGAATTGA TCAAACAGAT GGAGTGTATG CAACAAGGCG CCATCATGAA TCGTATAGAA GGTTTTCAGA GCGAATCTCG CCCTGTATTG CATACGGCAA CACGCGCTTG GGTTCGAGAT CAAGATCTTC ATGAAGAAGC TGCTGCGATA GCAAGACACT CTAAGGAGGA AGCTCTTCGT CTTGCAGAGT TTCTGTATAT AGCTCGTGCT AAATTTTCTA CGCTTGTACA GATGGGTATT GGGGGCTCGG AGTTGGGCCC TAAAGCTATG TATTTTGCTA TGCAAGGCTC TTGCCCATCT GATAAGAGAA TCTTTTTTGT CTCCAATATT GATCCGGATA ATGCTGCAGA AGTATTACGG GAGATAGATT TGGAGCAAAC ACTGGTTGTT GTGGTTTCTA AGTCAGGAAC AACTTTAGAG CCTGCAGCAA ATGAAGAGCT GTTCAGACAG GCTTATCAAA ATAAAGGTTT ATCTATTGCA GAGCATTTTG TAGCGGTAAC TTCTCAAGGC AGCCCTATGG ATGATAAGAG TCGTTATCTA GAAGTATTCC ATCTTTGGGA TAGTATTGGC GGAAGATTTT CAGCTACATC CATGGTAGGA GGAGTTGTAT TAGGTTTTGC TTTCGGTTAC GAAGCTTTTA TAGAGTTTTT GCAAGGAGCA GCTGCTATCG ATGCTCATGC GCTGACTCCA AAAATGAGAG AGAATCTTCC TCTTCTATCT GCGATGCTTG GAGTTTGGAA TCGTAATCTG TTAGGGTATC CTACAACAGC AGTGATTCCT TATTCGACAG GATTGAAATA TTTTACAGCG CATTTACAAC AGTGTGGAAT GGAATCCAAT GGAAAAAGTA TTTCGAGAGA AGGGAAGGAG ATTAGTTTTA GGACTTCACC CATTATTTGG GGCGATGTGG GGACAAACTG TCAGCATTCT TTTTTCCAAA GTCTTCATCA AGGAACAGAT ATTGTTCCCG TAGAGTTCAT TGGATTTTTG CATAACCAAC GAGGGCTAGA TTGTGTATTA TCAGGAAGCT CATCATCTCA AAAGCTCTTT GCAAACCTTG TTGCTCAATC TTTAGCTTTA GCTCAGGGTA GAGATAATGC GAACTCGAAT AAGAGGTTTA AAGGGAACAG ACCTTCTTCG ATATTGGTAG CGCAACAGCT TTCTCCTCGT ATTGCTGGTA GTTTATTGGC TTTTTATGAG CATAAATTTG CATTTCAAGG GTTTTGCTGG GGAATCAATT CTTTTGACCA AGAAGGGGTT TCATTAGGGA AAGAATTGGC AACGCAAATT ATAGGCATCA TGTCGGGGAA TGCTCCTGTA GAGTTTTCCG AAGCTCGAGG TATGTTAAGA CTTTTTAACG TTTTAACATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT