ChtrB.01427.a
Inorganic pyrophosphatase (EC 3.6.1.1) (Pyrophosphate phospho-hydrolase) (PPase)
| CENTER ID: | ChtrB.01427.a |
| ORGANISM: | Chlamydia trachomatis D/UW-3/Cx |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.01427.a.B1.GE42413 | full length | 1 | 209 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6WE5
DEPOSITED: 4/1/2020
DETERMINATION: XRay
CLONE: ChtrB.01427.a.B1.GE42413
PROTEIN: ChtrB.01427.a.B1.PW38676
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_82016 |
Publications by SSGCID
Crystal structure of an inorganic pyrophosphatase from Chlamydia trachomatis D/UW-3/Cx
Abendroth J, Edwards TE, Myler PJ, Staker BL, Subramanian S, Asojo OA, Hybiske K, Maddy J
Acta Crystallogr F Struct Biol Commun - 2022
volume 78, issue Pt 3, pages 135-142
PMID: 35234139; PMCID: PMC8900733
PDB:
6WE5
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272561.5.peg.848 |
| UniProt: | O84777 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKTPLSIAH PWHGPVLTRD DYESLCCYIE ITPADSVKFE LDKETGILKV DRPQKFSNFC PCLYGLLPKT YCGDLSGEYS GQQSNRENIK GDGDPLDICV LTEKNITQGN ILLQARPIGG IRILDSEEAD DKIIAVLEDD LVYGNIEDIS ECPGTVLDMI QHYFLTYKAT PESLIQAKPA KIEIVGLYGK KEAQKVIRLA HEDYCNLFM
NT Sequence
atgtctaaaa caccattatc catagctcat ccttggcatg ggccagtatt aacacgcgat gattatgaat ctctttgttg ctatatagaa atcactccag ccgactccgt taaattcgaa ctggataaag aaactggtat cctaaaagtg gatcggccac aaaagttttc taacttttgt ccttgcttat acgggctgtt acctaagact tattgtggag atctttctgg agaatacagt ggtcaacaaa gtaacagaga gaatatcaaa ggcgatggcg atcctcttga tatctgtgtg ttaacggaaa aaaatattac acaagggaat atcctcttgc aagcgcgtcc tatcggaggg attcgtattt tagactcgga agaagccgat gataaaatca tcgctgttct agaagatgat ttagtctatg gcaatataga agatatttct gaatgcccag gcacagtttt ggacatgatc caacactatt tcttaaccta taaagctact ccagaaagct taattcaagc aaaaccagct aaaattgaaa ttgtaggttt atacggcaaa aaagaagctc aaaaagtcat tcgtcttgct cacgaagact attgcaatct ttttatgtaa
Details for ChtrB.01427.a.B1.GE42413
| HARVESTED ON: | 5/25/2018 |
| SEQUENCED ON: | 5/31/2018 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHHM SKTPLSIAHP WHGPVLTRDD YESLCCYIEI TPADSVKFEL DKETGILKVD RPQKFSNFCP CLYGLLPKTY CGDLSGEYSG QQSNRENIKG DGDPLDICVL TEKNITQGNI LLQARPIGGI RILDSEEADD KIIAVLEDDL VYGNIEDISE CPGTVLDMIQ HYFLTYKATP ESLIQAKPAK IEIVGLYGKK EAQKVIRLAH EDYCNLFM
Validated NT Sequence
atcttactca cttacataaa aagattgcaa tagtcttcgt gagcaagacg aatgactttt tgagcttctt ttttgccgta taaacctaca atttcaattt tagctggttt tgcttgaatt aagctttctg gagtagcttt ataggttaag aaatagtgtt ggatcatgtc caaaactgtg cctgggcatt cagaaatatc ttctatattg ccatagacta aatcatcttc tagaacagcg atgattttat catcggcttc ttccgagtct aaaatacgaa tccctccgat aggacgcgct tgcaagagga tgttcccttg tgtaatattt ttttccgtta acacacagat atcaagagga tcgccatcgc ctttgatatt ctctctgtta ctttgttgac cactgtattc tccagaaaga tctccacaat aagtcttagg taacagcccg tataagcaag gacaaaagtt agaaaacttt tgtggccgat ccacttttag gataccagtt tctttatcca gttcgaattt aacggagtcg gctggagtga tttctatata gcaacaaaga gattcataat catcgcgtgt taatactggc ccatgccaag gatgagctat ggataatggt gttttagaca tatggtggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS KTPLSIAHPW HGPVLTRDDY ESLCCYIEIT PADSVKFELD KETGILKVDR PQKFSNFCPC LYGLLPKTYC GDLSGEYSGQ QSNRENIKGD GDPLDICVLT EKNITQGNIL LQARPIGGIR ILDSEEADDK IIAVLEDDLV YGNIEDISEC PGTVLDMIQH YFLTYKATPE SLIQAKPAKI EIVGLYGKKE AQKVIRLAHE DYCNLFM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta aaacaccatt atccatagct catccttggc atgggccagt attaacacgc gatgattatg aatctctttg ttgctatata gaaatcactc cagccgactc cgttaaattc gaactggata aagaaactgg tatcctaaaa gtggatcggc cacaaaagtt ttctaacttt tgtccttgct tatacgggct gttacctaag acttattgtg gagatctttc tggagaatac agtggtcaac aaagtaacag agagaatatc aaaggcgatg gcgatcctct tgatatctgt gtgttaacgg aaaaaaatat tacacaaggg aatatcctct tgcaagcgcg tcctatcgga gggattcgta ttttagactc ggaagaagcc gatgataaaa tcatcgctgt tctagaagat gatttagtct atggcaatat agaagatatt tctgaatgcc caggcacagt tttggacatg atccaacact atttcttaac ctataaagct actccagaaa gcttaattca agcaaaacca gctaaaattg aaattgtagg tttatacggc aaaaaagaag ctcaaaaagt cattcgtctt gctcacgaag actattgcaa tctttttatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat