ChtrB.01032.a

Tyrosine--tRNA ligase (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS)

CENTER ID: ChtrB.01032.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.01032.a.B1.GE42411 full length 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.01032.a.B1.PW38501 full length 1 412
Molecular models
METHOD RESULTS
comparative modelling Robetta_82007
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272561.5.peg.71
UniProt: O84065
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQLIDNLKK RGILDNSSAG LESLTVPVSA YLGFDPTAPS LHIGHWIGIC FLRRLAAYGI TPVALVGGAT GMIGDPSGKS VERSLLDQAQ VLDNSKKIAA ALASYLPGIR IVNNADWLGS LSMVDFLRDV GKHFRLGSML AKDVVKQRVY SEEGISYTEF SYLLLQSYDF AHLFKEHNVV LQCGGSDQWG NITSGIDYIR RRGLGQAYGL TYPLLTDSKG KKIGKTESGT IWLDPALTPP YELFQYFLRL PDQEISKVMR TLTLLDNEEI FALDERLTSD PQAVKKYIAE VIVKDVHGSE GLAQAQAATE SFFASKGKSI TEAELVALVE SGVGVKVARA DLIGKRWLDI VVELGFCSSR GQARRLIQQR GLYINQEPLA DEQSILDGTQ LCFDRYVLLS QGKRKKQVID LN
NT Sequence
atgcaacagt taatcgataa ccttaagaaa cggggtattc tagataattc ttctgcagga ttagaaagtt taacagttcc tgtttctgcc tatttagggt tcgatccaac tgcgccttct ttacacatag gacattggat tggaatttgt tttttgcgtc gattagcagc atatggaatc actcctgttg ctcttgttgg cggagctacc ggaatgatcg gagatccttc tggtaaaagt gtggagcgtt cattactaga tcaggcacag gtgcttgata atagtaagaa aatagcggct gctcttgcta gctatcttcc tggtatccgt attgtgaata atgcggattg gctaggatct ttaagtatgg tggatttttt aagagatgtt gggaagcatt ttcgtttagg ttctatgtta gctaaagacg tagtgaagca gcgagtctat tctgaagagg gaattagcta cactgagttc agttatttat tgctgcagtc ttatgatttt gcacatctct ttaaagagca taatgttgta ttacagtgtg gagggagtga tcagtggggg aatattactt cggggattga ttatatccgt cgaagaggac tagggcaggc ttatggtcta acctatcctt tgctcactga tagcaaaggg aagaaaatag ggaagacgga gtctggaact atctggctgg atccagcgtt aactcctcct tatgaactat tccaatattt cttacgcttg ccagatcaag aaatctccaa agtaatgaga actcttactc ttttggataa cgaagaaatt tttgctcttg atgagcgttt gactagtgat ccacaagctg tgaagaaata cattgcggaa gtgatcgtta aagatgttca tggttctgag ggattagctc aggctcaagc cgcaaccgaa agcttttttg ctagtaaggg aaagagtatt acagaagcag aactagtagc gttagtagag tcaggtgttg gcgttaaagt agctcgagca gatttaatag ggaaacgctg gttagatatc gttgtggaac taggcttttg ttcctcaaga ggacaagcta gaagactcat tcaacagcga ggtctgtaca tcaatcagga gcctttggcc gatgaacaga gtatattaga cgggactcag ttgtgtttcg atcgttatgt tttgttgtcc caagggaaaa gaaaaaaaca agtgatagat cttaattag
Details for ChtrB.01032.a.B1.GE42411
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ QLIDNLKKRG ILDNSSAGLE SLTVPVSAYL GFDPTAPSLH IGHWIGICFL RRLAAYGITP VALVGGATGM IGDPSGKSVE RSLLDQAQVL DNSKKIAAAL ASYLPGIRIV NNADWLGSLS MVDFLRDVGK HFRLGSMLAK DVVKQRVYSE EGISYTEFSY LLLQSYDFAH LFKEHNVVLQ CGGSDQWGNI TSGIDYIRRR GLGQAYGLTY PLLTDSKGKK IGKTESGTIW LDPALTPPYE LFQYFLRLPD QEISKVMRTL TLLDNEEIFA LDERLTSDPQ AVKKYIAEVI VKDVHGSEGL AQAQAATESF FASKGKSITE AELVALVESG VGVKVARADL IGKRWLDIVV ELGFCSSRGQ ARRLIQQRGL YINQEPLADE QSILDGTQLC FDRYVLLSQG KR
Validated NT Sequence
ttttcttttc ccttgggaca acaaaacata acgatcgaaa cacaactgag tcccgtctaa tatactctgt tcatcggcca aaggctcctg attgatgtac agacctcgct gttgaatgag tcttctagct tgtcctcttg aggaacaaaa gcctagttcc acaacgatat ctaaccagcg tttccctatt aaatctgctc gagctacttt aacgccaaca cctgactcta ctaacgctac tagttctgct tctgtaatac tctttccctt actagcaaaa aagctttcgg ttgcggcttg agcctgagct aatccttcag aaccatgaac atctttaacg atcacttccg caatgtattt cttcacagct tgtggatcac tagtcaaacg ctcatcaaga gcaaaaattt cttcgttatc caaaagagta agagttctca ttactttgga gatttcttga tctggcaagc gtaggaaata ttggaatagt tcataaggag gagttaacgc tggatccagc cagatagttc cagactccgt cttccctatt ttcttccctt tgctatcagt gagcaaagga taggttagac cataagcctg ccctagtcct cttcgacgga tataatcaat ccccgaagta atattccccc actgatcact ccctccacac tgtaatacaa cattatgctc tttaaagaga tgtgcaaaat cataagactg cagcaataaa taactgaact cagtgtagct aattccctct tcagaataga ctcgctgctt cactacgtct ttagctaaca tagaacctaa acggaaatgc ttcccaacat ctcttaaaaa atccaccata cttaaagatc ctagccaatc cgcattattc acaatacgga taccaggaag atagctagca agagcagccg ctattttctt actattatca agcacctgtg cctgatctag taatgaacgc tccacacttt taccagaagg atctccgatc attccggtag ctccgccaac aagagcaaca ggagtgattc catatgctgc taatcgacgc aaaaaacaaa ttccaatcca atgtcctatg tgtaaagaag gcgcagttgg atcgaaccct aaataggcag aaacaggaac tgttaaactt tctaatcctg cggaagaatt atctagaata ccccgtttct taaggttatc gattaactgt tgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMQ QLIDNLKKRG ILDNSSAGLE SLTVPVSAYL GFDPTAPSLH IGHWIGICFL RRLAAYGITP VALVGGATGM IGDPSGKSVE RSLLDQAQVL DNSKKIAAAL ASYLPGIRIV NNADWLGSLS MVDFLRDVGK HFRLGSMLAK DVVKQRVYSE EGISYTEFSY LLLQSYDFAH LFKEHNVVLQ CGGSDQWGNI TSGIDYIRRR GLGQAYGLTY PLLTDSKGKK IGKTESGTIW LDPALTPPYE LFQYFLRLPD QEISKVMRTL TLLDNEEIFA LDERLTSDPQ AVKKYIAEVI VKDVHGSEGL AQAQAATESF FASKGKSITE AELVALVESG VGVKVARADL IGKRWLDIVV ELGFCSSRGQ ARRLIQQRGL YINQEPLADE QSILDGTQLC FDRYVLLSQG KRKKQVIDLN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac agttaatcga taaccttaag aaacggggta ttctagataa ttcttctgca ggattagaaa gtttaacagt tcctgtttct gcctatttag ggttcgatcc aactgcgcct tctttacaca taggacattg gattggaatt tgttttttgc gtcgattagc agcatatgga atcactcctg ttgctcttgt tggcggagct accggaatga tcggagatcc ttctggtaaa agtgtggagc gttcattact agatcaggca caggtgcttg ataatagtaa gaaaatagcg gctgctcttg ctagctatct tcctggtatc cgtattgtga ataatgcgga ttggctagga tctttaagta tggtggattt tttaagagat gttgggaagc attttcgttt aggttctatg ttagctaaag acgtagtgaa gcagcgagtc tattctgaag agggaattag ctacactgag ttcagttatt tattgctgca gtcttatgat tttgcacatc tctttaaaga gcataatgtt gtattacagt gtggagggag tgatcagtgg gggaatatta cttcggggat tgattatatc cgtcgaagag gactagggca ggcttatggt ctaacctatc ctttgctcac tgatagcaaa gggaagaaaa tagggaagac ggagtctgga actatctggc tggatccagc gttaactcct ccttatgaac tattccaata tttcttacgc ttgccagatc aagaaatctc caaagtaatg agaactctta ctcttttgga taacgaagaa atttttgctc ttgatgagcg tttgactagt gatccacaag ctgtgaagaa atacattgcg gaagtgatcg ttaaagatgt tcatggttct gagggattag ctcaggctca agccgcaacc gaaagctttt ttgctagtaa gggaaagagt attacagaag cagaactagt agcgttagta gagtcaggtg ttggcgttaa agtagctcga gcagatttaa tagggaaacg ctggttagat atcgttgtgg aactaggctt ttgttcctca agaggacaag ctagaagact cattcaacag cgaggtctgt acatcaatca ggagcctttg gccgatgaac agagtatatt agacgggact cagttgtgtt tcgatcgtta tgttttgttg tcccaaggga aaagaaaaaa acaagtgata gatcttaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ChtrB.01032.a.B1.PW38501
PURIFICATION DATe: 7/2/2018
CONCENTRATION: 25.23mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ QLIDNLKKRG ILDNSSAGLE SLTVPVSAYL GFDPTAPSLH IGHWIGICFL RRLAAYGITP VALVGGATGM IGDPSGKSVE RSLLDQAQVL DNSKKIAAAL ASYLPGIRIV NNADWLGSLS MVDFLRDVGK HFRLGSMLAK DVVKQRVYSE EGISYTEFSY LLLQSYDFAH LFKEHNVVLQ CGGSDQWGNI TSGIDYIRRR GLGQAYGLTY PLLTDSKGKK IGKTESGTIW LDPALTPPYE LFQYFLRLPD QEISKVMRTL TLLDNEEIFA LDERLTSDPQ AVKKYIAEVI VKDVHGSEGL AQAQAATESF FASKGKSITE AELVALVESG VGVKVARADL IGKRWLDIVV ELGFCSSRGQ ARRLIQQRGL YINQEPLADE QSILDGTQLC FDRYVLLSQG KR
Validated NT Sequence
ttttcttttc ccttgggaca acaaaacata acgatcgaaa cacaactgag tcccgtctaa tatactctgt tcatcggcca aaggctcctg attgatgtac agacctcgct gttgaatgag tcttctagct tgtcctcttg aggaacaaaa gcctagttcc acaacgatat ctaaccagcg tttccctatt aaatctgctc gagctacttt aacgccaaca cctgactcta ctaacgctac tagttctgct tctgtaatac tctttccctt actagcaaaa aagctttcgg ttgcggcttg agcctgagct aatccttcag aaccatgaac atctttaacg atcacttccg caatgtattt cttcacagct tgtggatcac tagtcaaacg ctcatcaaga gcaaaaattt cttcgttatc caaaagagta agagttctca ttactttgga gatttcttga tctggcaagc gtaggaaata ttggaatagt tcataaggag gagttaacgc tggatccagc cagatagttc cagactccgt cttccctatt ttcttccctt tgctatcagt gagcaaagga taggttagac cataagcctg ccctagtcct cttcgacgga tataatcaat ccccgaagta atattccccc actgatcact ccctccacac tgtaatacaa cattatgctc tttaaagaga tgtgcaaaat cataagactg cagcaataaa taactgaact cagtgtagct aattccctct tcagaataga ctcgctgctt cactacgtct ttagctaaca tagaacctaa acggaaatgc ttcccaacat ctcttaaaaa atccaccata cttaaagatc ctagccaatc cgcattattc acaatacgga taccaggaag atagctagca agagcagccg ctattttctt actattatca agcacctgtg cctgatctag taatgaacgc tccacacttt taccagaagg atctccgatc attccggtag ctccgccaac aagagcaaca ggagtgattc catatgctgc taatcgacgc aaaaaacaaa ttccaatcca atgtcctatg tgtaaagaag gcgcagttgg atcgaaccct aaataggcag aaacaggaac tgttaaactt tctaatcctg cggaagaatt atctagaata ccccgtttct taaggttatc gattaactgt tgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMQ QLIDNLKKRG ILDNSSAGLE SLTVPVSAYL GFDPTAPSLH IGHWIGICFL RRLAAYGITP VALVGGATGM IGDPSGKSVE RSLLDQAQVL DNSKKIAAAL ASYLPGIRIV NNADWLGSLS MVDFLRDVGK HFRLGSMLAK DVVKQRVYSE EGISYTEFSY LLLQSYDFAH LFKEHNVVLQ CGGSDQWGNI TSGIDYIRRR GLGQAYGLTY PLLTDSKGKK IGKTESGTIW LDPALTPPYE LFQYFLRLPD QEISKVMRTL TLLDNEEIFA LDERLTSDPQ AVKKYIAEVI VKDVHGSEGL AQAQAATESF FASKGKSITE AELVALVESG VGVKVARADL IGKRWLDIVV ELGFCSSRGQ ARRLIQQRGL YINQEPLADE QSILDGTQLC FDRYVLLSQG KRKKQVIDLN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC AGTTAATCGA TAACCTTAAG AAACGGGGTA TTCTAGATAA TTCTTCTGCA GGATTAGAAA GTTTAACAGT TCCTGTTTCT GCCTATTTAG GGTTCGATCC AACTGCGCCT TCTTTACACA TAGGACATTG GATTGGAATT TGTTTTTTGC GTCGATTAGC AGCATATGGA ATCACTCCTG TTGCTCTTGT TGGCGGAGCT ACCGGAATGA TCGGAGATCC TTCTGGTAAA AGTGTGGAGC GTTCATTACT AGATCAGGCA CAGGTGCTTG ATAATAGTAA GAAAATAGCG GCTGCTCTTG CTAGCTATCT TCCTGGTATC CGTATTGTGA ATAATGCGGA TTGGCTAGGA TCTTTAAGTA TGGTGGATTT TTTAAGAGAT GTTGGGAAGC ATTTTCGTTT AGGTTCTATG TTAGCTAAAG ACGTAGTGAA GCAGCGAGTC TATTCTGAAG AGGGAATTAG CTACACTGAG TTCAGTTATT TATTGCTGCA GTCTTATGAT TTTGCACATC TCTTTAAAGA GCATAATGTT GTATTACAGT GTGGAGGGAG TGATCAGTGG GGGAATATTA CTTCGGGGAT TGATTATATC CGTCGAAGAG GACTAGGGCA GGCTTATGGT CTAACCTATC CTTTGCTCAC TGATAGCAAA GGGAAGAAAA TAGGGAAGAC GGAGTCTGGA ACTATCTGGC TGGATCCAGC GTTAACTCCT CCTTATGAAC TATTCCAATA TTTCTTACGC TTGCCAGATC AAGAAATCTC CAAAGTAATG AGAACTCTTA CTCTTTTGGA TAACGAAGAA ATTTTTGCTC TTGATGAGCG TTTGACTAGT GATCCACAAG CTGTGAAGAA ATACATTGCG GAAGTGATCG TTAAAGATGT TCATGGTTCT GAGGGATTAG CTCAGGCTCA AGCCGCAACC GAAAGCTTTT TTGCTAGTAA GGGAAAGAGT ATTACAGAAG CAGAACTAGT AGCGTTAGTA GAGTCAGGTG TTGGCGTTAA AGTAGCTCGA GCAGATTTAA TAGGGAAACG CTGGTTAGAT ATCGTTGTGG AACTAGGCTT TTGTTCCTCA AGAGGACAAG CTAGAAGACT CATTCAACAG CGAGGTCTGT ACATCAATCA GGAGCCTTTG GCCGATGAAC AGAGTATATT AGACGGGACT CAGTTGTGTT TCGATCGTTA TGTTTTGTTG TCCCAAGGGA AAAGAAAAAA ACAAGTGATA GATCTTAATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT