ChtrB.00944.a

Ribose-5-phosphate isomerase A (EC 5.3.1.6) (Phosphoriboisomerase A) (PRI)

CENTER ID: ChtrB.00944.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00944.a.AE1.GE44049 full length 1 242
ChtrB.00944.a.B1.GE42409 full length 1 242
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00944.a.B1.PW38524 full length 1 242
Molecular models
METHOD RESULTS
comparative modelling Robetta_82011
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272561.5.peg.228
UniProt: O84215
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKQPENSFS SDKFFPIKQK LALEAVALVE PGMCVGLGSG STAREFILAL GDRVRTERLV ITAVASSRIS QLLAEAVGIP LSDHSLLQDV DLVVDGADEV DPCLRMIKGG GGALFREKIL LQSGKRNVIL VDERKLVPTL GKFSLPIEIA PFGCSSVQRI LNKQGYFGEW RETSAGERFI TDNGNYIYDV RTPDSYANPE EDMIRLLQIR GIIDVGFVIA KAEVWVGYAD GSIVRKKEHN EY
NT Sequence
atgtctaaac aacccgagaa ctctttttct tccgacaaat ttttccctat caagcagaaa ctagcgctag aggcagtagc tttggtggag ccaggaatgt gtgtggggtt agggagcggc tcgacggcta gagagttcat tttggcttta ggagatcgtg ttcggacaga acgtttggta atcacagcgg tagcctcttc aaggatatct cagctattag ctgaagcagt ggggattcct ttatcggatc attctttatt gcaagatgta gacttggtcg tggatggagc ggatgaagtt gatccttgct tacgtatgat caaaggagga gggggagctc ttttccgaga aaaaattctt cttcagagcg ggaagcgcaa tgttattcta gtagatgaaa ggaaacttgt tcccacatta gggaagtttt cattgcctat agagattgct ccttttggtt gctcttctgt acagaggata ttgaataaac agggatactt tggagaatgg agagaaactt ctgcaggaga acgttttatc acggataatg gcaattatat ttacgatgtg cgtacacccg attcctatgc gaatcccgaa gaggatatga ttagactatt acaaattcgt ggcattattg atgtcgggtt tgttatagca aaagccgagg tgtgggtagg ttacgccgac ggttctattg tccgaaaaaa agaacataat gaatattga
Details for ChtrB.00944.a.AE1.GE44049
HARVESTED ON: 1/7/2021
SEQUENCED ON: 1/8/2021
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MSKQPENSFS SDKFFPIKQK LALEAVALVE PGMCVGLGSG STAREFILAL GDRVRTERLV ITAVASSRIS QLLAEAVGIP LSDHSLLQDV DLVVDGADEV DPCLRMIKGG GGALFREKIL LQSGKRNVIL VDERKLVPTL GKFSLPIEIA PFGCSSVQRI LNKQGYFGEW RETSAGERFI TDNGNYIYDV RTPDSYANPE EDMIRLLQIR GIIDVGFVIA KAEVWVGYAD GSIVRKKEHN EYAGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag catattcatt atgttctttt tttcggacaa tagaaccgtc ggcgtaacct acccacacct cggcttttgc tataacaaac ccgacatcaa taatgccacg aatttgtaat agtctaatca tatcctcttc gggattcgca taggaatcgg gtgtacgcac atcgtaaata taattgccat tatccgtgat aaaacgttct cctgcagaag tttctctcca ttctccaaag tatccctgtt tattcaatat cctctgtaca gaagagcaac caaaaggagc aatctctata ggcaatgaaa acttccctaa tgtgggaaca agtttccttt catctactag aataacattg cgcttcccgc tctgaagaag aattttttct cggaaaagag ctccccctcc tcctttgatc atacgtaagc aaggatcaac ttcatccgct ccatccacga ccaagtctac atcttgcaat aaagaatgat ccgataaagg aatccccact gcttcagcta atagctgaga tatccttgaa gaggctaccg ctgtgattac caaacgttct gtccgaacac gatctcctaa agccaaaatg aactctctag ccgtcgagcc gctccctaac cccacacaca ttcctggctc caccaaagct actgcctcta gcgctagttt ctgcttgata gggaaaaatt tgtcggaaga aaaagagttc tcgggttgtt tagacatggg agaga
Expected Protein Sequence
MSKQPENSFS SDKFFPIKQK LALEAVALVE PGMCVGLGSG STAREFILAL GDRVRTERLV ITAVASSRIS QLLAEAVGIP LSDHSLLQDV DLVVDGADEV DPCLRMIKGG GGALFREKIL LQSGKRNVIL VDERKLVPTL GKFSLPIEIA PFGCSSVQRI LNKQGYFGEW RETSAGERFI TDNGNYIYDV RTPDSYANPE EDMIRLLQIR GIIDVGFVIA KAEVWVGYAD GSIVRKKEHN EYGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtctaaacaa cccgagaact ctttttcttc cgacaaattt ttccctatca agcagaaact agcgctagag gcagtagctt tggtggagcc aggaatgtgt gtggggttag ggagcggctc gacggctaga gagttcattt tggctttagg agatcgtgtt cggacagaac gtttggtaat cacagcggta gcctcttcaa ggatatctca gctattagct gaagcagtgg ggattccttt atcggatcat tctttattgc aagatgtaga cttggtcgtg gatggagcgg atgaagttga tccttgctta cgtatgatca aaggaggagg gggagctctt ttccgagaaa aaattcttct tcagagcggg aagcgcaatg ttattctagt agatgaaagg aaacttgttc ccacattagg gaagttttca ttgcctatag agattgctcc ttttggttgc tcttctgtac agaggatatt gaataaacag ggatactttg gagaatggag agaaacttct gcaggagaac gttttatcac ggataatggc aattatattt acgatgtgcg tacacccgat tcctatgcga atcccgaaga ggatatgatt agactattac aaattcgtgg cattattgat gtcgggtttg ttatagcaaa agccgaggtg tgggtaggtt acgccgacgg ttctattgtc cgaaaaaaag aacataatga atatgggcac caccatcatc atcattaacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
Details for ChtrB.00944.a.B1.GE42409
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS KQPENSFSSD KFFPIKQKLA LEAVALVEPG MCVGLGSGST AREFILALGD RVRTERLVIT AVASSRISQL LAEAVGIPLL DHSLLQDVDL VVDGADEVDP CLRMIKGGGG ALFREKILLQ SGKRNVILVD ERKLVPTLGK FSLPIEIAPF GCSSVQRILN KQGYFGEWRE TSAGERFITD NGNYIYDVRT PDSYANPEED MIRLLQIRGI IDVGFVIAKA EVWVGYADGS IVRKKEHNEY
Validated NT Sequence
atcttactca cttaatattc attatgttct ttttttcgga caatagaacc gtcggcgtaa cctacccaca cctcggcttt tgctataaca aacccgacat caataatgcc acgaatttgt aatagtctaa tcatatcctc ttcgggattc gcataggaat cgggtgtacg cacatcgtaa atataattgc cattatccgt gataaaacgt tctcctgcag aagtttctct ccattctcca aagtatccct gtttattcaa tatcctctgt acagaagagc aaccaaaagg agcaatctct ataggcaatg aaaacttccc taatgtggga acaagtttcc tttcatctac tagaataaca ttgcgcttcc cgctctgaag aagaattttt tctcggaaaa gagctccccc tcctcctttg atcatacgta agcaaggatc aacttcatcc gctccatcca cgaccaagtc tacatcttgc aataaagaat gatccaataa aggaatcccc actgcttcag ctaatagctg agatatcctt gaagaggcta ccgctgtgat taccaaacgt tctgtccgaa cacgatctcc taaagccaaa atgaactctc tagccgtcga gccgctccct aaccccacac acattcctgg ctccaccaaa gctactgcct ctagcgctag tttctgcttg atagggaaaa atttgtcgga agaaaaagag ttctcgggtt gtttagacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS KQPENSFSSD KFFPIKQKLA LEAVALVEPG MCVGLGSGST AREFILALGD RVRTERLVIT AVASSRISQL LAEAVGIPLS DHSLLQDVDL VVDGADEVDP CLRMIKGGGG ALFREKILLQ SGKRNVILVD ERKLVPTLGK FSLPIEIAPF GCSSVQRILN KQGYFGEWRE TSAGERFITD NGNYIYDVRT PDSYANPEED MIRLLQIRGI IDVGFVIAKA EVWVGYADGS IVRKKEHNEY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta aacaacccga gaactctttt tcttccgaca aatttttccc tatcaagcag aaactagcgc tagaggcagt agctttggtg gagccaggaa tgtgtgtggg gttagggagc ggctcgacgg ctagagagtt cattttggct ttaggagatc gtgttcggac agaacgtttg gtaatcacag cggtagcctc ttcaaggata tctcagctat tagctgaagc agtggggatt cctttatcgg atcattcttt attgcaagat gtagacttgg tcgtggatgg agcggatgaa gttgatcctt gcttacgtat gatcaaagga ggagggggag ctcttttccg agaaaaaatt cttcttcaga gcgggaagcg caatgttatt ctagtagatg aaaggaaact tgttcccaca ttagggaagt tttcattgcc tatagagatt gctccttttg gttgctcttc tgtacagagg atattgaata aacagggata ctttggagaa tggagagaaa cttctgcagg agaacgtttt atcacggata atggcaatta tatttacgat gtgcgtacac ccgattccta tgcgaatccc gaagaggata tgattagact attacaaatt cgtggcatta ttgatgtcgg gtttgttata gcaaaagccg aggtgtgggt aggttacgcc gacggttcta ttgtccgaaa aaaagaacat aatgaatatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ChtrB.00944.a.B1.PW38524
PURIFICATION DATe: 9/18/2018
CONCENTRATION: 14.44mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS KQPENSFSSD KFFPIKQKLA LEAVALVEPG MCVGLGSGST AREFILALGD RVRTERLVIT AVASSRISQL LAEAVGIPLL DHSLLQDVDL VVDGADEVDP CLRMIKGGGG ALFREKILLQ SGKRNVILVD ERKLVPTLGK FSLPIEIAPF GCSSVQRILN KQGYFGEWRE TSAGERFITD NGNYIYDVRT PDSYANPEED MIRLLQIRGI IDVGFVIAKA EVWVGYADGS IVRKKEHNEY
Validated NT Sequence
atcttactca cttaatattc attatgttct ttttttcgga caatagaacc gtcggcgtaa cctacccaca cctcggcttt tgctataaca aacccgacat caataatgcc acgaatttgt aatagtctaa tcatatcctc ttcgggattc gcataggaat cgggtgtacg cacatcgtaa atataattgc cattatccgt gataaaacgt tctcctgcag aagtttctct ccattctcca aagtatccct gtttattcaa tatcctctgt acagaagagc aaccaaaagg agcaatctct ataggcaatg aaaacttccc taatgtggga acaagtttcc tttcatctac tagaataaca ttgcgcttcc cgctctgaag aagaattttt tctcggaaaa gagctccccc tcctcctttg atcatacgta agcaaggatc aacttcatcc gctccatcca cgaccaagtc tacatcttgc aataaagaat gatccaataa aggaatcccc actgcttcag ctaatagctg agatatcctt gaagaggcta ccgctgtgat taccaaacgt tctgtccgaa cacgatctcc taaagccaaa atgaactctc tagccgtcga gccgctccct aaccccacac acattcctgg ctccaccaaa gctactgcct ctagcgctag tttctgcttg atagggaaaa atttgtcgga agaaaaagag ttctcgggtt gtttagacat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMS KQPENSFSSD KFFPIKQKLA LEAVALVEPG MCVGLGSGST AREFILALGD RVRTERLVIT AVASSRISQL LAEAVGIPLS DHSLLQDVDL VVDGADEVDP CLRMIKGGGG ALFREKILLQ SGKRNVILVD ERKLVPTLGK FSLPIEIAPF GCSSVQRILN KQGYFGEWRE TSAGERFITD NGNYIYDVRT PDSYANPEED MIRLLQIRGI IDVGFVIAKA EVWVGYADGS IVRKKEHNEY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTA AACAACCCGA GAACTCTTTT TCTTCCGACA AATTTTTCCC TATCAAGCAG AAACTAGCGC TAGAGGCAGT AGCTTTGGTG GAGCCAGGAA TGTGTGTGGG GTTAGGGAGC GGCTCGACGG CTAGAGAGTT CATTTTGGCT TTAGGAGATC GTGTTCGGAC AGAACGTTTG GTAATCACAG CGGTAGCCTC TTCAAGGATA TCTCAGCTAT TAGCTGAAGC AGTGGGGATT CCTTTATCGG ATCATTCTTT ATTGCAAGAT GTAGACTTGG TCGTGGATGG AGCGGATGAA GTTGATCCTT GCTTACGTAT GATCAAAGGA GGAGGGGGAG CTCTTTTCCG AGAAAAAATT CTTCTTCAGA GCGGGAAGCG CAATGTTATT CTAGTAGATG AAAGGAAACT TGTTCCCACA TTAGGGAAGT TTTCATTGCC TATAGAGATT GCTCCTTTTG GTTGCTCTTC TGTACAGAGG ATATTGAATA AACAGGGATA CTTTGGAGAA TGGAGAGAAA CTTCTGCAGG AGAACGTTTT ATCACGGATA ATGGCAATTA TATTTACGAT GTGCGTACAC CCGATTCCTA TGCGAATCCC GAAGAGGATA TGATTAGACT ATTACAAATT CGTGGCATTA TTGATGTCGG GTTTGTTATA GCAAAAGCCG AGGTGTGGGT AGGTTACGCC GACGGTTCTA TTGTCCGAAA AAAAGAACAT AATGAATATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT