ChtrB.00925.a

Probable fructose-bisphosphate aldolase class 1 (EC 4.1.2.13) (Probable fructose-bisphosphate aldolase class I) (FBP aldolase)

CENTER ID: ChtrB.00925.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00925.a.B1.GE42408 full length 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00925.a.B1.PS38528 full length 1 348
Molecular models
METHOD RESULTS
comparative modelling Robetta_82017
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272561.5.peg.230
UniProt: O84217
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTIFDLLGK DADYLLNHKC VIKKEALTLP SGDLVSRVFA ESDRNNRVLR SLQQMFSCGR LGGTGYLSIL PVDQGVEHTA GASFAKNPMY FDPENIVRLA MEAGCSAVAS SYGVLSILAR RYAHKIPFLL KLNHNELLSY PTTYHQIFFS QVEDAYNMGA VAVGATIYFG SESSSEEIVA VAEAFARARE LGLATVLWCY LRNPHFVVNN VDYHTAADLT GQADHLGATL GADIVKQKLP TLQGGFKTIN FSKTDDLVYS ELSSNHPIDL CRYQVLNSYC GKVGLINSGG PSGQDDFAEA VKTAVINKRA GGMGLILGRK AFQRPFSEGV RLLNLIQDIY LDPTISIS
NT Sequence
atgacaacga tttttgatct tcttggtaaa gacgccgact atctccttaa ccacaagtgt gttataaaaa aagaagcttt aacgcttcct tctggagacc tcgtatcacg agtttttgca gaatcagaca gaaataatcg ggtgctccgt tcccttcaac agatgttcag ttgtggacgc ttaggcggaa caggatacct ctctattctc cctgttgacc aaggtgtcga acatactgca ggcgcatcgt ttgccaagaa tcctatgtac tttgatccag aaaatatcgt gcgtctggct atggaagctg gctgttccgc ggtagcttct tcctatggcg tgttaagtat attggctaga cgatatgctc ataaaattcc ttttttactt aaactaaacc acaacgaact gctatcctac ccgacaacat atcaccaaat cttcttcagt caagttgaag atgcctataa tatgggagct gttgctgtcg gagcaacgat ttactttgga tcagagtctt cttctgaaga aattgttgct gttgccgaag cttttgcaag agctagagag cttggcttgg ctacagtgtt atggtgctat ttaaggaatc cacattttgt tgtgaataat gtggattacc acactgctgc cgatttaaca gggcaagcgg accatcttgg agcaacttta ggagctgata tcgttaagca aaagctccca actcttcaag gaggatttaa aacaattaat ttcagcaaga ctgatgatct tgtgtactcg gagctttcat caaaccaccc tattgacctc tgtcggtacc aggtacttaa tagttactgt ggaaaggttg gtctaattaa ctccggaggc ccttctgggc aagatgactt tgctgaagca gttaaaacag cagtaattaa taaacgagct ggtggcatgg gactgatttt agggagaaaa gctttccaga gacccttctc tgaaggcgtc cggttactca atttaattca agatatttat ttagatccga caatctccat atcataa
Details for ChtrB.00925.a.B1.GE42408
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMT TIFDLLGKDA DYLLNHKCVI KKEALTLPSG DFVSRVFAES DRNNRVLRSL QQMFSYGRLG GTGYLSILPV DQGVEHTAGA SFAKNPMYFD PENIVRLAME AGCSAVASSY GVLSILARRY AHKIPFLLKL NHNELLSYPT TYHQIFFSQV EDAYNMGAVA VGATIYFGSE SSSEEIVAVA EAFARARELG LATVLWCYLR NPHFVVNNVD YHTAADLTGQ ADHLGATLGA DIVKQKLPTL QGGFKTINFS KTDDLVYSEL SSNHPIDLCR YQVLNSYCGK VGLINSGGPS GQDDFAEAAK TAVINKRAGG MGLILGRKAF QRPLSEGVRL LNLIQDIYXX XTIS
Validated NT Sequence
atggagattg tnnnnnnnaa ataaatatct tgaattaaat tgagtaaccg gacgccttca gagaggggtc tctggaaagc ttttctccct aaaatcagtc ccatgccacc agctcgttta ttaattactg ctgttttagc tgcttcagca aagtcatctt gcccagaagg gcctccggag ttaattagac caacctttcc acagtaacta ttaagtacct ggtaccgaca gaggtcaata gggtggtttg atgaaagctc cgagtacaca agatcatcag tcttgctgaa attaattgtt ttaaatcctc cttgaagagt tgggagcttt tgcttaacga tatcagctcc taaagttgct ccaagatggt ccgcttgccc tgttaaatcg gcagcagtgt ggtaatccac attattcaca acaaaatgtg gattccttaa atagcaccat aacactgtag ccaagccaag ctctctagct cttgcaaaag cttcggcaac agcaacaatt tcttcagaag aagactctga tccaaagtaa atcgttgctc cgacagcaac agctcccata ttataggcat cttcaacttg actgaagaag atttggtgat atgttgtcgg gtaggatagc agttcgttgt ggtttagttt aagtaaaaaa ggaattttat gagcatatcg tctagccaat atacttaaca cgccatagga agaagctaca gcggaacagc cagcttccat agccagacgc acgatatttt ctggatcaaa gtacatagga ttcttggcaa acgatgcgcc tgcagtatgt tcgacacctt ggtcaacagg gagaatagag aggtatcctg ttccgcctaa gcgtccataa ctgaacatct gttgaaggga acggagcacc cgattatttc tgtctgattc tgcaaaaact cgtgatacga agtctccaga aggaagcgtt aaagcttctt tttttataac acacttgtgg ttaaggagat agtcggcgtc tttaccaaga agatcaaaaa tcgttgtcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMT TIFDLLGKDA DYLLNHKCVI KKEALTLPSG DLVSRVFAES DRNNRVLRSL QQMFSCGRLG GTGYLSILPV DQGVEHTAGA SFAKNPMYFD PENIVRLAME AGCSAVASSY GVLSILARRY AHKIPFLLKL NHNELLSYPT TYHQIFFSQV EDAYNMGAVA VGATIYFGSE SSSEEIVAVA EAFARARELG LATVLWCYLR NPHFVVNNVD YHTAADLTGQ ADHLGATLGA DIVKQKLPTL QGGFKTINFS KTDDLVYSEL SSNHPIDLCR YQVLNSYCGK VGLINSGGPS GQDDFAEAVK TAVINKRAGG MGLILGRKAF QRPFSEGVRL LNLIQDIYLD PTISIS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa cgatttttga tcttcttggt aaagacgccg actatctcct taaccacaag tgtgttataa aaaaagaagc tttaacgctt ccttctggag acctcgtatc acgagttttt gcagaatcag acagaaataa tcgggtgctc cgttcccttc aacagatgtt cagttgtgga cgcttaggcg gaacaggata cctctctatt ctccctgttg accaaggtgt cgaacatact gcaggcgcat cgtttgccaa gaatcctatg tactttgatc cagaaaatat cgtgcgtctg gctatggaag ctggctgttc cgcggtagct tcttcctatg gcgtgttaag tatattggct agacgatatg ctcataaaat tcctttttta cttaaactaa accacaacga actgctatcc tacccgacaa catatcacca aatcttcttc agtcaagttg aagatgccta taatatggga gctgttgctg tcggagcaac gatttacttt ggatcagagt cttcttctga agaaattgtt gctgttgccg aagcttttgc aagagctaga gagcttggct tggctacagt gttatggtgc tatttaagga atccacattt tgttgtgaat aatgtggatt accacactgc tgccgattta acagggcaag cggaccatct tggagcaact ttaggagctg atatcgttaa gcaaaagctc ccaactcttc aaggaggatt taaaacaatt aatttcagca agactgatga tcttgtgtac tcggagcttt catcaaacca ccctattgac ctctgtcggt accaggtact taatagttac tgtggaaagg ttggtctaat taactccgga ggcccttctg ggcaagatga ctttgctgaa gcagttaaaa cagcagtaat taataaacga gctggtggca tgggactgat tttagggaga aaagctttcc agagaccctt ctctgaaggc gtccggttac tcaatttaat tcaagatatt tatttagatc cgacaatctc catatcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for ChtrB.00925.a.B1.PS38528
PURIFICATION DATe: 11/6/2018
CONCENTRATION: 2.18mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TIFDLLGKDA DYLLNHKCVI KKEALTLPSG DFVSRVFAES DRNNRVLRSL QQMFSYGRLG GTGYLSILPV DQGVEHTAGA SFAKNPMYFD PENIVRLAME AGCSAVASSY GVLSILARRY AHKIPFLLKL NHNELLSYPT TYHQIFFSQV EDAYNMGAVA VGATIYFGSE SSSEEIVAVA EAFARARELG LATVLWCYLR NPHFVVNNVD YHTAADLTGQ ADHLGATLGA DIVKQKLPTL QGGFKTINFS KTDDLVYSEL SSNHPIDLCR YQVLNSYCGK VGLINSGGPS GQDDFAEAAK TAVINKRAGG MGLILGRKAF QRPLSEGVRL LNLIQDIYXX XTIS
Validated NT Sequence
atggagattg tnnnnnnnaa ataaatatct tgaattaaat tgagtaaccg gacgccttca gagaggggtc tctggaaagc ttttctccct aaaatcagtc ccatgccacc agctcgttta ttaattactg ctgttttagc tgcttcagca aagtcatctt gcccagaagg gcctccggag ttaattagac caacctttcc acagtaacta ttaagtacct ggtaccgaca gaggtcaata gggtggtttg atgaaagctc cgagtacaca agatcatcag tcttgctgaa attaattgtt ttaaatcctc cttgaagagt tgggagcttt tgcttaacga tatcagctcc taaagttgct ccaagatggt ccgcttgccc tgttaaatcg gcagcagtgt ggtaatccac attattcaca acaaaatgtg gattccttaa atagcaccat aacactgtag ccaagccaag ctctctagct cttgcaaaag cttcggcaac agcaacaatt tcttcagaag aagactctga tccaaagtaa atcgttgctc cgacagcaac agctcccata ttataggcat cttcaacttg actgaagaag atttggtgat atgttgtcgg gtaggatagc agttcgttgt ggtttagttt aagtaaaaaa ggaattttat gagcatatcg tctagccaat atacttaaca cgccatagga agaagctaca gcggaacagc cagcttccat agccagacgc acgatatttt ctggatcaaa gtacatagga ttcttggcaa acgatgcgcc tgcagtatgt tcgacacctt ggtcaacagg gagaatagag aggtatcctg ttccgcctaa gcgtccataa ctgaacatct gttgaaggga acggagcacc cgattatttc tgtctgattc tgcaaaaact cgtgatacga agtctccaga aggaagcgtt aaagcttctt tttttataac acacttgtgg ttaaggagat agtcggcgtc tttaccaaga agatcaaaaa tcgttgtcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMT TIFDLLGKDA DYLLNHKCVI KKEALTLPSG DLVSRVFAES DRNNRVLRSL QQMFSCGRLG GTGYLSILPV DQGVEHTAGA SFAKNPMYFD PENIVRLAME AGCSAVASSY GVLSILARRY AHKIPFLLKL NHNELLSYPT TYHQIFFSQV EDAYNMGAVA VGATIYFGSE SSSEEIVAVA EAFARARELG LATVLWCYLR NPHFVVNNVD YHTAADLTGQ ADHLGATLGA DIVKQKLPTL QGGFKTINFS KTDDLVYSEL SSNHPIDLCR YQVLNSYCGK VGLINSGGPS GQDDFAEAVK TAVINKRAGG MGLILGRKAF QRPFSEGVRL LNLIQDIYLD PTISIS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA CGATTTTTGA TCTTCTTGGT AAAGACGCCG ACTATCTCCT TAACCACAAG TGTGTTATAA AAAAAGAAGC TTTAACGCTT CCTTCTGGAG ACCTCGTATC ACGAGTTTTT GCAGAATCAG ACAGAAATAA TCGGGTGCTC CGTTCCCTTC AACAGATGTT CAGTTGTGGA CGCTTAGGCG GAACAGGATA CCTCTCTATT CTCCCTGTTG ACCAAGGTGT CGAACATACT GCAGGCGCAT CGTTTGCCAA GAATCCTATG TACTTTGATC CAGAAAATAT CGTGCGTCTG GCTATGGAAG CTGGCTGTTC CGCGGTAGCT TCTTCCTATG GCGTGTTAAG TATATTGGCT AGACGATATG CTCATAAAAT TCCTTTTTTA CTTAAACTAA ACCACAACGA ACTGCTATCC TACCCGACAA CATATCACCA AATCTTCTTC AGTCAAGTTG AAGATGCCTA TAATATGGGA GCTGTTGCTG TCGGAGCAAC GATTTACTTT GGATCAGAGT CTTCTTCTGA AGAAATTGTT GCTGTTGCCG AAGCTTTTGC AAGAGCTAGA GAGCTTGGCT TGGCTACAGT GTTATGGTGC TATTTAAGGA ATCCACATTT TGTTGTGAAT AATGTGGATT ACCACACTGC TGCCGATTTA ACAGGGCAAG CGGACCATCT TGGAGCAACT TTAGGAGCTG ATATCGTTAA GCAAAAGCTC CCAACTCTTC AAGGAGGATT TAAAACAATT AATTTCAGCA AGACTGATGA TCTTGTGTAC TCGGAGCTTT CATCAAACCA CCCTATTGAC CTCTGTCGGT ACCAGGTACT TAATAGTTAC TGTGGAAAGG TTGGTCTAAT TAACTCCGGA GGCCCTTCTG GGCAAGATGA CTTTGCTGAA GCAGTTAAAA CAGCAGTAAT TAATAAACGA GCTGGTGGCA TGGGACTGAT TTTAGGGAGA AAAGCTTTCC AGAGACCCTT CTCTGAAGGC GTCCGGTTAC TCAATTTAAT TCAAGATATT TATTTAGATC CGACAATCTC CATATCATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT