ChtrB.00839.a
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (NAD-dependent glyceraldehyde-3-phosphate dehydrogenase)
| CENTER ID: | ChtrB.00839.a |
| ORGANISM: | Chlamydia trachomatis D/UW-3/Cx |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00839.a.B1.GE42407 | full length | 1 | 334 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00839.a.B1.PW38497 | full length | 1 | 334 |
Structures
6OK4
DEPOSITED: 4/12/2019
DETERMINATION: XRay
CLONE: ChtrB.00839.a.B1.GE42407
PROTEIN: ChtrB.00839.a.B1.PW38497
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_82012 |
Publications by SSGCID
Structures of glyceraldehyde 3-phosphate dehydrogenase in Neisseria gonorrhoeae and Chlamydia trachomatis
Abendroth J, Barrett LK, Choi R, Dranow DM, Edwards TE, Fan E, Lorimer DD, Myler P, Phan I, Staker B, Subramanian S, Van Voorhis WC, Zhang Z, Craig JC, Ojo KK, Horanyi PS, Barrett KF, Shaheen S, Hybiske K, Conrady DG, Navaluna ED, Michaels SA, Soge OO, Tillery L
Protein Sci. - 2020
volume 29, issue 3, pages 768-778
PMID: 31930578; PMCID: PMC7020975
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272561.5.peg.549 |
| UniProt: | P0CE13 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIVINGFGR IGRLVLRQIL KRNSPIEVVA INDLVAGDLL TYLFKYDSTH GSFAPQATFS DGCLVMGERK VHFLAEKDVQ KLPWKDLDVD VVVESTGLFV NRDDVAKHLD SGAKRVLITA PAKGDVPTFV MGVNHQQFDP ADVIISNASC TTNCLAPLAK VLLDNFGIEE GLMTTVHAAT ATQSVVDGPS RKDWRGGRGA FQNIIPASTG AAKAVGLCLP ELKGKLTGMA FRVPVADVSV VDLTVKLSSA TTYEAICEAV KHAANTSMKN IMYYTEEAVV SSDFIGCEYS SVFDAQAGVA LNDRFFKLVA WYDNEIGYAT RIVDLLEYVQ ENSK
NT Sequence
atgagaattg tgattaatgg ttttggacgg attgggcgat tagttttaag acagattctg aaaaggaatt ctcccataga agttgtagct attaatgatt tagtcgcagg agatctttta acatatttat ttaaatatga ttccacacac ggatctttcg ctcctcaagc aacattttcg gatggatgtt tggttatggg agaaagaaag gtccatttct tagcggaaaa agacgttcaa aagcttcctt ggaaggattt ggatgttgat gtcgtcgtcg aaagtactgg attgtttgtc aatagggatg atgttgcaaa gcatttggac tctggagcaa agagagtgtt gatcacagct cctgcgaaag gcgatgtccc tacgtttgtt atgggagtta accatcagca gtttgaccca gctgacgtca tcatttctaa tgcttcctgt accaccaatt gtttagctcc tttggccaaa gttctattgg ataattttgg tatagaagaa gggctaatga caacagttca cgctgcaaca gctacgcaga gtgtggttga tggcccttct cgtaaggatt ggagaggagg tagaggagct tttcagaata ttatcccggc ttcgacagga gctgctaaag ctgtagggtt gtgtttgcct gagcttaaag gaaaattaac aggaatggcc tttagagtgc ctgtagcaga tgtttctgta gtagatttaa ctgttaagtt gagctcagcc acgacgtacg aggctatctg tgaagctgtg aagcatgcag caaacacgag catgaagaat attatgtact acacggaaga agctgtagtc tcttctgatt ttattggctg tgagtattca tctgtattcg atgctcaagc cggggttgct ttgaacgatc gatttttcaa attggtagct tggtatgata atgaaatagg ctatgcaact cgcatagtgg atttattaga gtacgtacaa gaaaactcta aataa
Details for ChtrB.00839.a.B1.GE42407
| HARVESTED ON: | 5/25/2018 |
| SEQUENCED ON: | 5/31/2018 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR IVINGFGRIG RLVLRQILKR NSPIEVVAIN DLVAGDLLTY LFKYDSTHGS FAPQATFSDG CLVMGERKVH FLAEKDVQKL PWKDLDVDVV VESTGLFVNR DDVAKHLDSG AKRVLITAPA KGDVPTFVMG VNHQQFDPAD VIISNASCTT NCLAPLAKVL LDNFGIEEGL MTTVHAATAT QSVVDGPSRK DWRGGRGAFQ NIIPASTGAA KAVGLCLPEL KGKLTGMAFR VPVADVSVVD LTVKLSSATT YEAICEAVKH AANTSMKNIM YYTEEAVVSS DFIGCEYSSV FDAQAGVALN DRFFKLVAWY DNEIGYATRI VDLLEYVQEN SK
Validated NT Sequence
tctaataaat ccactatgcg agttgcatag cctatttcat tatcatacca agctaccaat ttgaaaaatc gatcgttcaa agcaaccccg gcttgagcat cgaatataga tgaatactca cagccaataa aatcagaaga gactacagct tcttccgtgt agtacataat attcttcatg ctcgtgtttg ctgcatgctt cacagcttca cagatagcct cgtacgtcgt ggctgagctc aacttaacag ttaaatctac tacagaaaca tctgctacag gcactctaaa ggccattcct gttaattttc ctttaagctc aggcaaacac aaccctacag ctttagcagc tcctgtcgaa gccgggataa tattctgaaa agctcctcta ccccctctcc aatccttacg agaagggcca tcaaccacac tctgcgtagc tgttgcagcg tgaactgttg tcattagccc ttcttctata ccaaaattat ccaatagaac tttggccaaa ggagctaaac aattggtagt acaggaagca ttagaaatga tgacgtcagc tgggtcaaac tgctgatggt taactcccat aacaaacgta gggacatcgc ctttcgcagg agctgtgatc aacactctct ttgctccaga gtccaaatgc tttgcagcat catccctatt gacaaacaat ccagtacttt cgacgacgac atcaacatcc aaatccttcc aaggaagctt ttgaacgtct ttttccgcta agaaacggat ctttctttct cccataacca aacatccatc cgaaaatgtt gcttgaggag cgaaagatcc gtgtgtggaa tcatatttaa ataaatatgt taaaagatct cctgcgacta aatcattaat agctacaact tctatgggag aattcctttt cagaatctgt cttaaaacta atcgcccaat ccgtccaaaa ccattaatca caattctcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMR IVINGFGRIG RLVLRQILKR NSPIEVVAIN DLVAGDLLTY LFKYDSTHGS FAPQATFSDG CLVMGERKVH FLAEKDVQKL PWKDLDVDVV VESTGLFVNR DDVAKHLDSG AKRVLITAPA KGDVPTFVMG VNHQQFDPAD VIISNASCTT NCLAPLAKVL LDNFGIEEGL MTTVHAATAT QSVVDGPSRK DWRGGRGAFQ NIIPASTGAA KAVGLCLPEL KGKLTGMAFR VPVADVSVVD LTVKLSSATT YEAICEAVKH AANTSMKNIM YYTEEAVVSS DFIGCEYSSV FDAQAGVALN DRFFKLVAWY DNEIGYATRI VDLLEYVQEN SK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa ttgtgattaa tggttttgga cggattgggc gattagtttt aagacagatt ctgaaaagga attctcccat agaagttgta gctattaatg atttagtcgc aggagatctt ttaacatatt tatttaaata tgattccaca cacggatctt tcgctcctca agcaacattt tcggatggat gtttggttat gggagaaaga aaggtccatt tcttagcgga aaaagacgtt caaaagcttc cttggaagga tttggatgtt gatgtcgtcg tcgaaagtac tggattgttt gtcaataggg atgatgttgc aaagcatttg gactctggag caaagagagt gttgatcaca gctcctgcga aaggcgatgt ccctacgttt gttatgggag ttaaccatca gcagtttgac ccagctgacg tcatcatttc taatgcttcc tgtaccacca attgtttagc tcctttggcc aaagttctat tggataattt tggtatagaa gaagggctaa tgacaacagt tcacgctgca acagctacgc agagtgtggt tgatggccct tctcgtaagg attggagagg aggtagagga gcttttcaga atattatccc ggcttcgaca ggagctgcta aagctgtagg gttgtgtttg cctgagctta aaggaaaatt aacaggaatg gcctttagag tgcctgtagc agatgtttct gtagtagatt taactgttaa gttgagctca gccacgacgt acgaggctat ctgtgaagct gtgaagcatg cagcaaacac gagcatgaag aatattatgt actacacgga agaagctgta gtctcttctg attttattgg ctgtgagtat tcatctgtat tcgatgctca agccggggtt gctttgaacg atcgattttt caaattggta gcttggtatg ataatgaaat aggctatgca actcgcatag tggatttatt agagtacgta caagaaaact ctaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ChtrB.00839.a.B1.PW38497
| PURIFICATION DATe: | 6/25/2018 |
| CONCENTRATION: | 17.29mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR IVINGFGRIG RLVLRQILKR NSPIEVVAIN DLVAGDLLTY LFKYDSTHGS FAPQATFSDG CLVMGERKVH FLAEKDVQKL PWKDLDVDVV VESTGLFVNR DDVAKHLDSG AKRVLITAPA KGDVPTFVMG VNHQQFDPAD VIISNASCTT NCLAPLAKVL LDNFGIEEGL MTTVHAATAT QSVVDGPSRK DWRGGRGAFQ NIIPASTGAA KAVGLCLPEL KGKLTGMAFR VPVADVSVVD LTVKLSSATT YEAICEAVKH AANTSMKNIM YYTEEAVVSS DFIGCEYSSV FDAQAGVALN DRFFKLVAWY DNEIGYATRI VDLLEYVQEN SK
Validated NT Sequence
tctaataaat ccactatgcg agttgcatag cctatttcat tatcatacca agctaccaat ttgaaaaatc gatcgttcaa agcaaccccg gcttgagcat cgaatataga tgaatactca cagccaataa aatcagaaga gactacagct tcttccgtgt agtacataat attcttcatg ctcgtgtttg ctgcatgctt cacagcttca cagatagcct cgtacgtcgt ggctgagctc aacttaacag ttaaatctac tacagaaaca tctgctacag gcactctaaa ggccattcct gttaattttc ctttaagctc aggcaaacac aaccctacag ctttagcagc tcctgtcgaa gccgggataa tattctgaaa agctcctcta ccccctctcc aatccttacg agaagggcca tcaaccacac tctgcgtagc tgttgcagcg tgaactgttg tcattagccc ttcttctata ccaaaattat ccaatagaac tttggccaaa ggagctaaac aattggtagt acaggaagca ttagaaatga tgacgtcagc tgggtcaaac tgctgatggt taactcccat aacaaacgta gggacatcgc ctttcgcagg agctgtgatc aacactctct ttgctccaga gtccaaatgc tttgcagcat catccctatt gacaaacaat ccagtacttt cgacgacgac atcaacatcc aaatccttcc aaggaagctt ttgaacgtct ttttccgcta agaaacggat ctttctttct cccataacca aacatccatc cgaaaatgtt gcttgaggag cgaaagatcc gtgtgtggaa tcatatttaa ataaatatgt taaaagatct cctgcgacta aatcattaat agctacaact tctatgggag aattcctttt cagaatctgt cttaaaacta atcgcccaat ccgtccaaaa ccattaatca caattctcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMR IVINGFGRIG RLVLRQILKR NSPIEVVAIN DLVAGDLLTY LFKYDSTHGS FAPQATFSDG CLVMGERKVH FLAEKDVQKL PWKDLDVDVV VESTGLFVNR DDVAKHLDSG AKRVLITAPA KGDVPTFVMG VNHQQFDPAD VIISNASCTT NCLAPLAKVL LDNFGIEEGL MTTVHAATAT QSVVDGPSRK DWRGGRGAFQ NIIPASTGAA KAVGLCLPEL KGKLTGMAFR VPVADVSVVD LTVKLSSATT YEAICEAVKH AANTSMKNIM YYTEEAVVSS DFIGCEYSSV FDAQAGVALN DRFFKLVAWY DNEIGYATRI VDLLEYVQEN SK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAA TTGTGATTAA TGGTTTTGGA CGGATTGGGC GATTAGTTTT AAGACAGATT CTGAAAAGGA ATTCTCCCAT AGAAGTTGTA GCTATTAATG ATTTAGTCGC AGGAGATCTT TTAACATATT TATTTAAATA TGATTCCACA CACGGATCTT TCGCTCCTCA AGCAACATTT TCGGATGGAT GTTTGGTTAT GGGAGAAAGA AAGGTCCATT TCTTAGCGGA AAAAGACGTT CAAAAGCTTC CTTGGAAGGA TTTGGATGTT GATGTCGTCG TCGAAAGTAC TGGATTGTTT GTCAATAGGG ATGATGTTGC AAAGCATTTG GACTCTGGAG CAAAGAGAGT GTTGATCACA GCTCCTGCGA AAGGCGATGT CCCTACGTTT GTTATGGGAG TTAACCATCA GCAGTTTGAC CCAGCTGACG TCATCATTTC TAATGCTTCC TGTACCACCA ATTGTTTAGC TCCTTTGGCC AAAGTTCTAT TGGATAATTT TGGTATAGAA GAAGGGCTAA TGACAACAGT TCACGCTGCA ACAGCTACGC AGAGTGTGGT TGATGGCCCT TCTCGTAAGG ATTGGAGAGG AGGTAGAGGA GCTTTTCAGA ATATTATCCC GGCTTCGACA GGAGCTGCTA AAGCTGTAGG GTTGTGTTTG CCTGAGCTTA AAGGAAAATT AACAGGAATG GCCTTTAGAG TGCCTGTAGC AGATGTTTCT GTAGTAGATT TAACTGTTAA GTTGAGCTCA GCCACGACGT ACGAGGCTAT CTGTGAAGCT GTGAAGCATG CAGCAAACAC GAGCATGAAG AATATTATGT ACTACACGGA AGAAGCTGTA GTCTCTTCTG ATTTTATTGG CTGTGAGTAT TCATCTGTAT TCGATGCTCA AGCCGGGGTT GCTTTGAACG ATCGATTTTT CAAATTGGTA GCTTGGTATG ATAATGAAAT AGGCTATGCA ACTCGCATAG TGGATTTATT AGAGTACGTA CAAGAAAACT CTAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT