ChtrB.00799.a
Probable cytosol aminopeptidase (EC 3.4.11.1) (Leucine aminopeptidase) (LAP) (EC 3.4.11.10) (Leucyl aminopeptidase)
| CENTER ID: | ChtrB.00799.a |
| ORGANISM: | Chlamydia trachomatis D/UW-3/Cx |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00799.a.B1.GE42406 | full length | 1 | 499 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00799.a.B1.PW38523 | full length | 1 | 499 |
Structures
6OME
DEPOSITED: 4/18/2019
DETERMINATION: XRay
CLONE: ChtrB.00799.a.B1.GE42406
PROTEIN: ChtrB.00799.a.B1.PW38523
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_82021 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272561.5.peg.51 |
| UniProt: | O84049 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLLYSQASW DKRSKADALV LPFWMKNSKA QEAAVVDEDY KLVYQNALSN FSGKKGETAF LFGNDHTKEQ KIVLLGLGKS EEVSGTTVLE AYAQATTVLR KAKCKTVNIL LPTISQLRFS VEEFLTNLAA GVLSLNYNYP TYHKVDTSLP FLEKVTVMGI VSKVGDKIFR KEESLFEGVY LTRDLVNTNA DEVTPEKLAA VAKDLAGEFA SLDVKILDRK AILKEKMGLL AAVAKGAAVE PRFIVLDYQG KPKSKDRTVL IGKGVTFDSG GLDLKPGKAM ITMKEDMAGA ATVLGIFSAL ASLELPINVT GIIPATENAI GSAAYKMGDV YVGMTGLSVE IGSTDAEGRL ILADAISYAL KYCNPTRIID FATLTGAMVV SLGESVAGFF ANNDVLARDL AEASSETGEA LWRMPLVEKY DQALHSDIAD MKNIGSNRAG SITAALFLQR FLEDNPVAWA HLDIAGTAYH EKEELPYPKY ATGFGVRCLI HYMEKFLSK
NT Sequence
gtggtattac tctattctca agcgagttgg gataaacgat caaaagcgga tgctcttgtt cttccttttt ggatgaagaa ttctaaagct caagaagctg cggttgttga tgaggactac aagcttgtct atcaaaacgc attatccaat ttttcaggga agaaagggga aacggctttt ctttttggaa atgatcacac aaaagaacaa aaaattgttc ttcttggtct agggaagagc gaagaagtat ccggaacaac cgttttagaa gcctacgctc aggctactac tgttttaaga aaagctaagt gtaagactgt aaatatttta ctcccaacaa tttcacagtt gcgcttctcc gtagaagagt ttttaacgaa cttggcagca ggggtgctat ctctgaacta taattaccca acctatcaca aagtggatac gtctttgcct ttcctagaga aagtgactgt aatgggtatt gtctctaagg taggggacaa gatctttaga aaagaagaga gcctatttga aggggtatat ttaactagag atttagtgaa taccaatgca gatgaagtca ctccagaaaa acttgctgcg gtagcaaaag atctagcagg ggagttcgcg agtctggatg taaaaattct agataggaag gcgatattaa aagaaaaaat gggattgttg gctgctgttg ccaagggcgc tgctgttgag cctcggttta ttgttctgga ttaccaaggt aaacctaaat ctaaagatag aaccgtactc attggtaaag gggtaacatt cgattccgga ggactagatt tgaaacctgg gaaggcaatg attaccatga aggaagacat ggctggagcg gctaccgttc taggaatttt ttctgcttta gcttccttag agcttccgat caatgtgacc gggatcattc cagctacaga gaatgcaatt ggatcggctg cctataagat gggagatgta tatgttggaa tgaccggcct ttctgtagaa attggcagca ctgatgcgga agggcgtttg attttagcag atgccatctc ctatgctttg aaatattgta atcctacccg catcattgac tttgctacct tgacgggtgc tatggttgtt tctttaggag aatctgtggc tggatttttt gcaaataacg acgtgttggc aagagatcta gcagaagctt catcagagac cggggaagct ctatggagaa tgcctttggt agagaaatat gaccaggcac ttcattcaga tattgcagat atgaaaaata tcggcagcaa tcgtgcagga tcgattactg cagcgctatt tttacaacgt ttcctcgaag acaatccagt agcatgggca catttggaca ttgcaggtac tgcttaccat gaaaaagaag agttgcctta ccccaaatat gcaacaggat ttggtgtgcg ttgtttaatt cattatatgg agaaattcct atctaaatag
Details for ChtrB.00799.a.B1.GE42406
| HARVESTED ON: | 5/25/2018 |
| SEQUENCED ON: | 5/31/2018 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMV VLLYSQASWD QRSKADALVL PFWMKNSKAQ EAAVVDEDYK LVYQNALSNF SGKKGETAFL FGNDHTKEQK IVLLGLGKSE EVSGTTVLEA YAQATTVLRK AKCKTVNILF PTISQLRFSV EEFLTNLAAG VLSLNYNYPT YHKVDTSLPF LEKVTVIGIV SKVGDKIFRK EESLFEGVYL TRDLVNTNAD EVTPEKLAAV AKGLAGEFAS LDVKILDRKA ILKEKMGLLA AVAKGAAVEP RFIVLDYQGK PKSKDRTVLI GKGVTFDSGG LDLKPGKAMI TMKEDMAGAA TVLGIFSALA SLELPINVTG IIPATENAIG SAAYKMGDVY VGMTGLSVEI GSTDAEGRLI LADAISYALK YCNPTRIIDF ATLTGAMVVS LGESVAGFFA NNDVLARDLA EASSETGEAL WRMPLVEKYD QALHSDIADM KNIGSNRAGS ITAALFLQRF LEDNPVAWAH LDIAGTAYHE KEELPYPKYA TGFGVRCLIH
Validated NT Sequence
aatgaattaa acaacgcaca ccaaatcctg ttgcatattt ggggtaaggc aactcttctt tttcatggta agcagtaccg gcaatgtcca aatgtgccca tgctactgga ttgtcttcga ggaaacgttg taaaaatagc gctgcagtaa tcgatcctgc acgattgctg ccgatatttt tcatatctgc aatatctgaa tgaagtgcct ggtcatattt ctctaccaaa ggcattctcc atagagcttc cccggtctct gatgaagctt ctgcaagatc tcttgccaac acgtcgttat ttgcaaaaaa tccagccaca gattctccta aagaaacaac catagcaccc gttaaggtag caaagtcaat gatgcgggta ggattacaat atttcaaagc ataggagatg gcatctgcta aaatcaaacg cccttccgca tcagtgctgc caatttctac agaaaggccg gtcattccaa catatacatc tcccatctta taggcagccg atccaatcgc attctctgta gctggaatga tcccggtcac attgatcgga agctccaagg aagctaaagc agaaaaaatt cctagaacgg tagccgctcc agccatgtct tccttcatgg taatcattgc cttcccaggt ttcaaatcta gtcctccgga atcgaatgtt acccctttac caatgagtac ggttctatct ttagatttag gtttaccttg gtaatccaga acaataaacc gaggctcaac agcagcgccc ttggcaacag cagccaacaa tcccattttt tcttttaata tcgccttcct atctagaatt tttacatcca gactcgcgaa ctcccctgct agaccttttg ctaccgcagc aagtttttct ggagtgactt catctgcatt ggtattcact aaatctctag ttaaatatac cccttcaaat aggctctctt cttttctaaa gatcttgtcc cctaccttag agacaatacc tattacagtc actttctcta ggaaaggcaa agacgtatcc actttgtgat aggttgggta attatagttc agagatagca cccctgctgc caagttcgtt aaaaactctt ctacggagaa gcgcaactgt gaaattgttg ggaataaaat atttacagtc ttacacttag cttttcttaa aacagtagta gcctgagcgt aggcttctaa aacggttgtt ccggatactt cttcgctctt ccctagacca agaagaacaa ttttttgttc ttttgtgtga tcatttccaa aaagaaaagc tgtttcccct ttcttccctg aaaaattgga taatgcgttt tgatagacaa gcttgtagtc ctcatcaaca actgcagctt cttgagcttt agaattcttc atccaaaaag gaagaacaag agcatccgct ttcgatcgtt gatcccaact cgcttgagaa tagagtaata ccaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMV LLYSQASWDK RSKADALVLP FWMKNSKAQE AAVVDEDYKL VYQNALSNFS GKKGETAFLF GNDHTKEQKI VLLGLGKSEE VSGTTVLEAY AQATTVLRKA KCKTVNILLP TISQLRFSVE EFLTNLAAGV LSLNYNYPTY HKVDTSLPFL EKVTVMGIVS KVGDKIFRKE ESLFEGVYLT RDLVNTNADE VTPEKLAAVA KDLAGEFASL DVKILDRKAI LKEKMGLLAA VAKGAAVEPR FIVLDYQGKP KSKDRTVLIG KGVTFDSGGL DLKPGKAMIT MKEDMAGAAT VLGIFSALAS LELPINVTGI IPATENAIGS AAYKMGDVYV GMTGLSVEIG STDAEGRLIL ADAISYALKY CNPTRIIDFA TLTGAMVVSL GESVAGFFAN NDVLARDLAE ASSETGEALW RMPLVEKYDQ ALHSDIADMK NIGSNRAGSI TAALFLQRFL EDNPVAWAHL DIAGTAYHEK EELPYPKYAT GFGVRCLIHY MEKFLSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggtat tactctattc tcaagcgagt tgggataaac gatcaaaagc ggatgctctt gttcttcctt tttggatgaa gaattctaaa gctcaagaag ctgcggttgt tgatgaggac tacaagcttg tctatcaaaa cgcattatcc aatttttcag ggaagaaagg ggaaacggct tttctttttg gaaatgatca cacaaaagaa caaaaaattg ttcttcttgg tctagggaag agcgaagaag tatccggaac aaccgtttta gaagcctacg ctcaggctac tactgtttta agaaaagcta agtgtaagac tgtaaatatt ttactcccaa caatttcaca gttgcgcttc tccgtagaag agtttttaac gaacttggca gcaggggtgc tatctctgaa ctataattac ccaacctatc acaaagtgga tacgtctttg cctttcctag agaaagtgac tgtaatgggt attgtctcta aggtagggga caagatcttt agaaaagaag agagcctatt tgaaggggta tatttaacta gagatttagt gaataccaat gcagatgaag tcactccaga aaaacttgct gcggtagcaa aagatctagc aggggagttc gcgagtctgg atgtaaaaat tctagatagg aaggcgatat taaaagaaaa aatgggattg ttggctgctg ttgccaaggg cgctgctgtt gagcctcggt ttattgttct ggattaccaa ggtaaaccta aatctaaaga tagaaccgta ctcattggta aaggggtaac attcgattcc ggaggactag atttgaaacc tgggaaggca atgattacca tgaaggaaga catggctgga gcggctaccg ttctaggaat tttttctgct ttagcttcct tagagcttcc gatcaatgtg accgggatca ttccagctac agagaatgca attggatcgg ctgcctataa gatgggagat gtatatgttg gaatgaccgg cctttctgta gaaattggca gcactgatgc ggaagggcgt ttgattttag cagatgccat ctcctatgct ttgaaatatt gtaatcctac ccgcatcatt gactttgcta ccttgacggg tgctatggtt gtttctttag gagaatctgt ggctggattt tttgcaaata acgacgtgtt ggcaagagat ctagcagaag cttcatcaga gaccggggaa gctctatgga gaatgccttt ggtagagaaa tatgaccagg cacttcattc agatattgca gatatgaaaa atatcggcag caatcgtgca ggatcgatta ctgcagcgct atttttacaa cgtttcctcg aagacaatcc agtagcatgg gcacatttgg acattgcagg tactgcttac catgaaaaag aagagttgcc ttaccccaaa tatgcaacag gatttggtgt gcgttgttta attcattata tggagaaatt cctatctaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for ChtrB.00799.a.B1.PW38523
| PURIFICATION DATe: | 9/18/2018 |
| CONCENTRATION: | 16.07mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 140µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV VLLYSQASWD QRSKADALVL PFWMKNSKAQ EAAVVDEDYK LVYQNALSNF SGKKGETAFL FGNDHTKEQK IVLLGLGKSE EVSGTTVLEA YAQATTVLRK AKCKTVNILF PTISQLRFSV EEFLTNLAAG VLSLNYNYPT YHKVDTSLPF LEKVTVIGIV SKVGDKIFRK EESLFEGVYL TRDLVNTNAD EVTPEKLAAV AKGLAGEFAS LDVKILDRKA ILKEKMGLLA AVAKGAAVEP RFIVLDYQGK PKSKDRTVLI GKGVTFDSGG LDLKPGKAMI TMKEDMAGAA TVLGIFSALA SLELPINVTG IIPATENAIG SAAYKMGDVY VGMTGLSVEI GSTDAEGRLI LADAISYALK YCNPTRIIDF ATLTGAMVVS LGESVAGFFA NNDVLARDLA EASSETGEAL WRMPLVEKYD QALHSDIADM KNIGSNRAGS ITAALFLQRF LEDNPVAWAH LDIAGTAYHE KEELPYPKYA TGFGVRCLIH
Validated NT Sequence
aatgaattaa acaacgcaca ccaaatcctg ttgcatattt ggggtaaggc aactcttctt tttcatggta agcagtaccg gcaatgtcca aatgtgccca tgctactgga ttgtcttcga ggaaacgttg taaaaatagc gctgcagtaa tcgatcctgc acgattgctg ccgatatttt tcatatctgc aatatctgaa tgaagtgcct ggtcatattt ctctaccaaa ggcattctcc atagagcttc cccggtctct gatgaagctt ctgcaagatc tcttgccaac acgtcgttat ttgcaaaaaa tccagccaca gattctccta aagaaacaac catagcaccc gttaaggtag caaagtcaat gatgcgggta ggattacaat atttcaaagc ataggagatg gcatctgcta aaatcaaacg cccttccgca tcagtgctgc caatttctac agaaaggccg gtcattccaa catatacatc tcccatctta taggcagccg atccaatcgc attctctgta gctggaatga tcccggtcac attgatcgga agctccaagg aagctaaagc agaaaaaatt cctagaacgg tagccgctcc agccatgtct tccttcatgg taatcattgc cttcccaggt ttcaaatcta gtcctccgga atcgaatgtt acccctttac caatgagtac ggttctatct ttagatttag gtttaccttg gtaatccaga acaataaacc gaggctcaac agcagcgccc ttggcaacag cagccaacaa tcccattttt tcttttaata tcgccttcct atctagaatt tttacatcca gactcgcgaa ctcccctgct agaccttttg ctaccgcagc aagtttttct ggagtgactt catctgcatt ggtattcact aaatctctag ttaaatatac cccttcaaat aggctctctt cttttctaaa gatcttgtcc cctaccttag agacaatacc tattacagtc actttctcta ggaaaggcaa agacgtatcc actttgtgat aggttgggta attatagttc agagatagca cccctgctgc caagttcgtt aaaaactctt ctacggagaa gcgcaactgt gaaattgttg ggaataaaat atttacagtc ttacacttag cttttcttaa aacagtagta gcctgagcgt aggcttctaa aacggttgtt ccggatactt cttcgctctt ccctagacca agaagaacaa ttttttgttc ttttgtgtga tcatttccaa aaagaaaagc tgtttcccct ttcttccctg aaaaattgga taatgcgttt tgatagacaa gcttgtagtc ctcatcaaca actgcagctt cttgagcttt agaattcttc atccaaaaag gaagaacaag agcatccgct ttcgatcgtt gatcccaact cgcttgagaa tagagtaata ccaccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMV LLYSQASWDK RSKADALVLP FWMKNSKAQE AAVVDEDYKL VYQNALSNFS GKKGETAFLF GNDHTKEQKI VLLGLGKSEE VSGTTVLEAY AQATTVLRKA KCKTVNILLP TISQLRFSVE EFLTNLAAGV LSLNYNYPTY HKVDTSLPFL EKVTVMGIVS KVGDKIFRKE ESLFEGVYLT RDLVNTNADE VTPEKLAAVA KDLAGEFASL DVKILDRKAI LKEKMGLLAA VAKGAAVEPR FIVLDYQGKP KSKDRTVLIG KGVTFDSGGL DLKPGKAMIT MKEDMAGAAT VLGIFSALAS LELPINVTGI IPATENAIGS AAYKMGDVYV GMTGLSVEIG STDAEGRLIL ADAISYALKY CNPTRIIDFA TLTGAMVVSL GESVAGFFAN NDVLARDLAE ASSETGEALW RMPLVEKYDQ ALHSDIADMK NIGSNRAGSI TAALFLQRFL EDNPVAWAHL DIAGTAYHEK EELPYPKYAT GFGVRCLIHY MEKFLSK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGTAT TACTCTATTC TCAAGCGAGT TGGGATAAAC GATCAAAAGC GGATGCTCTT GTTCTTCCTT TTTGGATGAA GAATTCTAAA GCTCAAGAAG CTGCGGTTGT TGATGAGGAC TACAAGCTTG TCTATCAAAA CGCATTATCC AATTTTTCAG GGAAGAAAGG GGAAACGGCT TTTCTTTTTG GAAATGATCA CACAAAAGAA CAAAAAATTG TTCTTCTTGG TCTAGGGAAG AGCGAAGAAG TATCCGGAAC AACCGTTTTA GAAGCCTACG CTCAGGCTAC TACTGTTTTA AGAAAAGCTA AGTGTAAGAC TGTAAATATT TTACTCCCAA CAATTTCACA GTTGCGCTTC TCCGTAGAAG AGTTTTTAAC GAACTTGGCA GCAGGGGTGC TATCTCTGAA CTATAATTAC CCAACCTATC ACAAAGTGGA TACGTCTTTG CCTTTCCTAG AGAAAGTGAC TGTAATGGGT ATTGTCTCTA AGGTAGGGGA CAAGATCTTT AGAAAAGAAG AGAGCCTATT TGAAGGGGTA TATTTAACTA GAGATTTAGT GAATACCAAT GCAGATGAAG TCACTCCAGA AAAACTTGCT GCGGTAGCAA AAGATCTAGC AGGGGAGTTC GCGAGTCTGG ATGTAAAAAT TCTAGATAGG AAGGCGATAT TAAAAGAAAA AATGGGATTG TTGGCTGCTG TTGCCAAGGG CGCTGCTGTT GAGCCTCGGT TTATTGTTCT GGATTACCAA GGTAAACCTA AATCTAAAGA TAGAACCGTA CTCATTGGTA AAGGGGTAAC ATTCGATTCC GGAGGACTAG ATTTGAAACC TGGGAAGGCA ATGATTACCA TGAAGGAAGA CATGGCTGGA GCGGCTACCG TTCTAGGAAT TTTTTCTGCT TTAGCTTCCT TAGAGCTTCC GATCAATGTG ACCGGGATCA TTCCAGCTAC AGAGAATGCA ATTGGATCGG CTGCCTATAA GATGGGAGAT GTATATGTTG GAATGACCGG CCTTTCTGTA GAAATTGGCA GCACTGATGC GGAAGGGCGT TTGATTTTAG CAGATGCCAT CTCCTATGCT TTGAAATATT GTAATCCTAC CCGCATCATT GACTTTGCTA CCTTGACGGG TGCTATGGTT GTTTCTTTAG GAGAATCTGT GGCTGGATTT TTTGCAAATA ACGACGTGTT GGCAAGAGAT CTAGCAGAAG CTTCATCAGA GACCGGGGAA GCTCTATGGA GAATGCCTTT GGTAGAGAAA TATGACCAGG CACTTCATTC AGATATTGCA GATATGAAAA ATATCGGCAG CAATCGTGCA GGATCGATTA CTGCAGCGCT ATTTTTACAA CGTTTCCTCG AAGACAATCC AGTAGCATGG GCACATTTGG ACATTGCAGG TACTGCTTAC CATGAAAAAG AAGAGTTGCC TTACCCCAAA TATGCAACAG GATTTGGTGT GCGTTGTTTA ATTCATTATA TGGAGAAATT CCTATCTAAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT