ChtrB.00743.a
Tryptophan--tRNA ligase (EC 6.1.1.2) (Tryptophanyl-tRNA synthetase) (TrpRS)
| CENTER ID: | ChtrB.00743.a |
| ORGANISM: | Chlamydia trachomatis D/UW-3/Cx |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00743.a.B1.GE42405 | full length | 1 | 346 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00743.a.B1.PW38496 | full length | 1 | 346 |
Structures
6NCR
DEPOSITED: 12/12/2018
DETERMINATION: XRay
CLONE: ChtrB.00743.a.B1.GE42405
PROTEIN: ChtrB.00743.a.B1.PW38496
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_82025 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272561.5.peg.637 |
| UniProt: | O84589 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKKRVLTGD RPTGKLHLGH WIGSIMNRLQ LQNDSRYDCF FIIADLHTLT TKTRKEEILQ IDNHIYDVLA DWLSVGIDPE KSAIYLQSAI PEIYELNLIF SMLTPLNHIM GIPSIKEMAR NASLNEESLS HGLIGYPVLQ SADILLAKAH LVPVGKDNEA HVELTRDIAK TFNRLYGEVF PEPDILQGEL TALVGTNGQG KMSKSANNAI YLSDDAKTVQ EKIRKLYTDP NRIHATTPGR VEGNPLFIYH DLFNPHKEEV EEFKTRYRQG CIRDVEVKAR LAEEINLFLN PFREKRSELV AQPKFLEEAL QQGTEKMRTV ARETMEEVHD HLGLSRKWRT ILASSK
NT Sequence
atgaaaaaaa aacgcgttct taccggagat cgccctactg ggaaacttca cctagggcat tggatcggct ccattatgaa tcgtctccag ctacaaaatg actctcgcta tgattgtttc ttcattatag cagatttgca cacgctaact actaaaacgc gcaaagaaga aatccttcaa atagataatc atatttatga cgttcttgcc gactggctta gcgtaggaat tgatcctgaa aaatccgcaa tctacctgca atcggctatc cctgagattt atgaattaaa tctcattttt tccatgctta ccccgttgaa tcacattatg ggtattccta gcatcaaaga aatggcccgt aatgcttctc taaatgaaga aagcctatcg catggactta taggataccc tgtattacaa agtgcagata ttctacttgc taaagcacat cttgttcctg ttgggaaaga taatgaggct catgtagagc tgactcgtga tattgccaaa acattcaatc ggctctatgg ggaagtgttt ccagaacccg atattctgca aggagaactg acagcccttg taggaaccaa tggtcaaggg aaaatgagta aatccgcgaa taacgccatt tatctttcgg atgacgcaaa gacagtacaa gaaaaaatac gaaaactgta cacggatcct aatcggattc acgcgacaac cccaggaaga gtggaaggga accctttatt tatctaccac gatcttttta atcctcataa ggaagaagta gaagagttta agactcgcta tcgtcaagga tgcattagag atgtagaagt aaaagctcgt ttagcagaag aaatcaatct tttcttaaat cctttccgag aaaaacgtag tgaattagtt gctcagccta aatttctcga ggaagccttg caacaaggaa cggaaaaaat gcgtaccgta gctcgagaaa ccatggaaga ggtgcatgac catctaggat taagccggaa atggcggact attctagcct cttctaaata a
Details for ChtrB.00743.a.B1.GE42405
| HARVESTED ON: | 5/25/2018 |
| SEQUENCED ON: | 5/31/2018 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK KKRVLTGDRP TGKLHLGHWI GSIMNRLQLQ NDSRYDCFFI IADLHTLTTK TRKEEVLQID NHIYDVLADW LSVGINPEKS AIYLQSAIPE IYELNLIFSM LTPLNHIMGI PSIKEMASNA SLNEESLSHG LIGYPVLQSA DILLAKAHLV PVGKDNEAHV ELTRDIAKTF NRLYGEVFPE PDILQGELTA LVGTNGQGKM SKSANNAIYL SDDAKTVQEK IRKLYTDPNR IHATTPGRVE GNPLFIYHDL FNPHKEEVEE FKTRYRQGCI KDVEVKARLA EEINLFLNPF REKRSELVAQ PKFLEEALQQ GTEKMRTVAR ETMEEVHDHL GLSRKW
Validated NT Sequence
gccatttccg gcttaatcct agatggtcat gcacctcttc catggtttct cgagctacgg tacgcatttt ttccgttcct tgttgcaagg cttcctcgag aaatttaggc tgagcaacta attcactacg tttttctcgg aaaggattta agaaaagatt gatttcttct gctaaacgag cttttacttc tacatcttta atgcatcctt gacgatagcg agtcttaaac tcttctactt cttccttatg aggattaaaa agatcgtggt agataaataa agggttccct tccactcttc ctggggttgt cgcgtgaatc cgattaggat ccgtgtatag ttttcgtatt ttttcttgta ctgtctttgc gtcatccgaa agataaatgg cgttattcgc ggatttactc attttccctt gaccattggt tcctacaagg gctgtcagtt ctccttgcag aatatcgggt tctggaaaca cttccccata gagccgattg aatgttttgg caatatcacg agtcagctct acatgagcct cattatcttt cccaacagga acaagatgtg ctttagcaag tagaatatct gcactttgta atacagggta tcctataagt ccatgcgata ggctttcttc atttagagaa gcattgctgg ccatttcttt gatgctagga atacccataa tgtgattcaa cggggtaagc atggaaaaaa tgagatttaa ttcataaatc tcagggatag ccgattgcag gtagattgcg gatttttcag gattaattcc tacgctaagc cagtcggcaa gaacgtcata aatatgatta tctatttgaa ggacttcttc tttgcgcgtt ttagtagtta gcgtgtgcaa atctgctata atgaagaaac aatcatagcg agagtcattt tgtagctgga gacgattcat aatggagccg atccaatgcc ctaggtgaag tttcccagta gggcgatctc cggtaagaac gcgttttttt ttcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMK KKRVLTGDRP TGKLHLGHWI GSIMNRLQLQ NDSRYDCFFI IADLHTLTTK TRKEEILQID NHIYDVLADW LSVGIDPEKS AIYLQSAIPE IYELNLIFSM LTPLNHIMGI PSIKEMARNA SLNEESLSHG LIGYPVLQSA DILLAKAHLV PVGKDNEAHV ELTRDIAKTF NRLYGEVFPE PDILQGELTA LVGTNGQGKM SKSANNAIYL SDDAKTVQEK IRKLYTDPNR IHATTPGRVE GNPLFIYHDL FNPHKEEVEE FKTRYRQGCI RDVEVKARLA EEINLFLNPF REKRSELVAQ PKFLEEALQQ GTEKMRTVAR ETMEEVHDHL GLSRKWRTIL ASSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaaaacgcgt tcttaccgga gatcgcccta ctgggaaact tcacctaggg cattggatcg gctccattat gaatcgtctc cagctacaaa atgactctcg ctatgattgt ttcttcatta tagcagattt gcacacgcta actactaaaa cgcgcaaaga agaaatcctt caaatagata atcatattta tgacgttctt gccgactggc ttagcgtagg aattgatcct gaaaaatccg caatctacct gcaatcggct atccctgaga tttatgaatt aaatctcatt ttttccatgc ttaccccgtt gaatcacatt atgggtattc ctagcatcaa agaaatggcc cgtaatgctt ctctaaatga agaaagccta tcgcatggac ttataggata ccctgtatta caaagtgcag atattctact tgctaaagca catcttgttc ctgttgggaa agataatgag gctcatgtag agctgactcg tgatattgcc aaaacattca atcggctcta tggggaagtg tttccagaac ccgatattct gcaaggagaa ctgacagccc ttgtaggaac caatggtcaa gggaaaatga gtaaatccgc gaataacgcc atttatcttt cggatgacgc aaagacagta caagaaaaaa tacgaaaact gtacacggat cctaatcgga ttcacgcgac aaccccagga agagtggaag ggaacccttt atttatctac cacgatcttt ttaatcctca taaggaagaa gtagaagagt ttaagactcg ctatcgtcaa ggatgcatta gagatgtaga agtaaaagct cgtttagcag aagaaatcaa tcttttctta aatcctttcc gagaaaaacg tagtgaatta gttgctcagc ctaaatttct cgaggaagcc ttgcaacaag gaacggaaaa aatgcgtacc gtagctcgag aaaccatgga agaggtgcat gaccatctag gattaagccg gaaatggcgg actattctag cctcttctaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ChtrB.00743.a.B1.PW38496
| PURIFICATION DATe: | 6/25/2018 |
| CONCENTRATION: | 17.16mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 29 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK KKRVLTGDRP TGKLHLGHWI GSIMNRLQLQ NDSRYDCFFI IADLHTLTTK TRKEEVLQID NHIYDVLADW LSVGINPEKS AIYLQSAIPE IYELNLIFSM LTPLNHIMGI PSIKEMASNA SLNEESLSHG LIGYPVLQSA DILLAKAHLV PVGKDNEAHV ELTRDIAKTF NRLYGEVFPE PDILQGELTA LVGTNGQGKM SKSANNAIYL SDDAKTVQEK IRKLYTDPNR IHATTPGRVE GNPLFIYHDL FNPHKEEVEE FKTRYRQGCI KDVEVKARLA EEINLFLNPF REKRSELVAQ PKFLEEALQQ GTEKMRTVAR ETMEEVHDHL GLSRKW
Validated NT Sequence
gccatttccg gcttaatcct agatggtcat gcacctcttc catggtttct cgagctacgg tacgcatttt ttccgttcct tgttgcaagg cttcctcgag aaatttaggc tgagcaacta attcactacg tttttctcgg aaaggattta agaaaagatt gatttcttct gctaaacgag cttttacttc tacatcttta atgcatcctt gacgatagcg agtcttaaac tcttctactt cttccttatg aggattaaaa agatcgtggt agataaataa agggttccct tccactcttc ctggggttgt cgcgtgaatc cgattaggat ccgtgtatag ttttcgtatt ttttcttgta ctgtctttgc gtcatccgaa agataaatgg cgttattcgc ggatttactc attttccctt gaccattggt tcctacaagg gctgtcagtt ctccttgcag aatatcgggt tctggaaaca cttccccata gagccgattg aatgttttgg caatatcacg agtcagctct acatgagcct cattatcttt cccaacagga acaagatgtg ctttagcaag tagaatatct gcactttgta atacagggta tcctataagt ccatgcgata ggctttcttc atttagagaa gcattgctgg ccatttcttt gatgctagga atacccataa tgtgattcaa cggggtaagc atggaaaaaa tgagatttaa ttcataaatc tcagggatag ccgattgcag gtagattgcg gatttttcag gattaattcc tacgctaagc cagtcggcaa gaacgtcata aatatgatta tctatttgaa ggacttcttc tttgcgcgtt ttagtagtta gcgtgtgcaa atctgctata atgaagaaac aatcatagcg agagtcattt tgtagctgga gacgattcat aatggagccg atccaatgcc ctaggtgaag tttcccagta gggcgatctc cggtaagaac gcgttttttt ttcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMK KKRVLTGDRP TGKLHLGHWI GSIMNRLQLQ NDSRYDCFFI IADLHTLTTK TRKEEILQID NHIYDVLADW LSVGIDPEKS AIYLQSAIPE IYELNLIFSM LTPLNHIMGI PSIKEMARNA SLNEESLSHG LIGYPVLQSA DILLAKAHLV PVGKDNEAHV ELTRDIAKTF NRLYGEVFPE PDILQGELTA LVGTNGQGKM SKSANNAIYL SDDAKTVQEK IRKLYTDPNR IHATTPGRVE GNPLFIYHDL FNPHKEEVEE FKTRYRQGCI RDVEVKARLA EEINLFLNPF REKRSELVAQ PKFLEEALQQ GTEKMRTVAR ETMEEVHDHL GLSRKWRTIL ASSK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA AAAAACGCGT TCTTACCGGA GATCGCCCTA CTGGGAAACT TCACCTAGGG CATTGGATCG GCTCCATTAT GAATCGTCTC CAGCTACAAA ATGACTCTCG CTATGATTGT TTCTTCATTA TAGCAGATTT GCACACGCTA ACTACTAAAA CGCGCAAAGA AGAAATCCTT CAAATAGATA ATCATATTTA TGACGTTCTT GCCGACTGGC TTAGCGTAGG AATTGATCCT GAAAAATCCG CAATCTACCT GCAATCGGCT ATCCCTGAGA TTTATGAATT AAATCTCATT TTTTCCATGC TTACCCCGTT GAATCACATT ATGGGTATTC CTAGCATCAA AGAAATGGCC CGTAATGCTT CTCTAAATGA AGAAAGCCTA TCGCATGGAC TTATAGGATA CCCTGTATTA CAAAGTGCAG ATATTCTACT TGCTAAAGCA CATCTTGTTC CTGTTGGGAA AGATAATGAG GCTCATGTAG AGCTGACTCG TGATATTGCC AAAACATTCA ATCGGCTCTA TGGGGAAGTG TTTCCAGAAC CCGATATTCT GCAAGGAGAA CTGACAGCCC TTGTAGGAAC CAATGGTCAA GGGAAAATGA GTAAATCCGC GAATAACGCC ATTTATCTTT CGGATGACGC AAAGACAGTA CAAGAAAAAA TACGAAAACT GTACACGGAT CCTAATCGGA TTCACGCGAC AACCCCAGGA AGAGTGGAAG GGAACCCTTT ATTTATCTAC CACGATCTTT TTAATCCTCA TAAGGAAGAA GTAGAAGAGT TTAAGACTCG CTATCGTCAA GGATGCATTA GAGATGTAGA AGTAAAAGCT CGTTTAGCAG AAGAAATCAA TCTTTTCTTA AATCCTTTCC GAGAAAAACG TAGTGAATTA GTTGCTCAGC CTAAATTTCT CGAGGAAGCC TTGCAACAAG GAACGGAAAA AATGCGTACC GTAGCTCGAG AAACCATGGA AGAGGTGCAT GACCATCTAG GATTAAGCCG GAAATGGCGG ACTATTCTAG CCTCTTCTAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT