ChtrB.00612.a

Lysine--tRNA ligase (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS)

CENTER ID: ChtrB.00612.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00612.a.B1.GE42404 full length 1 526
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_82003
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272561.5.peg.858
UniProt: O84786
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVEVEYLQH EDYLYRTSKL KEIRDLGINP YPYQYTDCLE VQEIRNQFVD NELGDSEAAF RKETPKVRFA GRLVLFRSMG KNSFGQILDN DAKIQVMFNR DFSAVAGLAA DAGISPIKFI EKKLDLGDIL GLEGYLFFTH SGELTVLVET VTLLCKSLIS LPDKHAGLAD KEIRYRKRWA DLISSEDVRK TFLTRSRILK LIREYMDQQS FLEVETPILQ TIYGGAEATP FVTTLQALHA EMFLRISLEI ALKKLLVGGM SRVYEIGKVF RNEGIDRTHN PEFTMIEAYA AYWDYNDVMK CVENLVEYIV RALNNGETQV QYSHLKSGPQ VVDFKAPWIR MTMKESISVY GGVDVDLHAD HELRKILETQ TSLPEKTYVH ASRGELIALL FDELVCDKLI APHHITDHPL ETTPLCKTLR SGDETLVERF ESFCLGKELC NAYSELNDPL QQRKLLEEQM RKKALNPDSE YHPIDEEFLE ALCQGMPPAG GFGIGIDRLV MMLTDAASIR DVLFFPVMRR IEAKKD
NT Sequence
atgtctgtag aagttgaata cttgcaacac gaagattatt tgtatagaac aagcaagcta aaggaaatca gagatttggg cataaatcct tacccttatc aatacactga ttgtcttgaa gtacaggaaa ttcgtaatca gtttgtagat aacgaattag gagatagcga agcggctttt cgtaaagaga cacctaaggt gcgttttgcc ggacgacttg ttcttttccg ttctatgggg aaaaattctt ttgggcagat cctcgataat gatgcaaaga ttcaagtgat gtttaatcga gatttttctg cagtggcagg gttagccgcg gatgctggga tttctccgat taaatttatt gagaagaaac ttgatctagg agacatcttg ggtctcgaag ggtatctttt ctttactcac tcaggagaat taacggttct cgttgaaacg gtaacgttgt tatgtaaatc cttaatttct ttgcctgata agcatgcagg attagcagat aaagaaattc gctatcgcaa acgttgggca gatctgattt cctcagagga tgtgcgtaag actttcttaa caagaagccg gattcttaag ttgattcgtg agtacatgga tcagcagagc tttttagagg tggaaactcc tatcctgcaa accatctacg gaggagcaga agcaactcct tttgttacca cgctgcaagc gctacatgca gaaatgttcc taagaatttc tctagagatt gctttgaaaa aactccttgt tggaggaatg tcccgagttt atgaaatcgg caaagttttc cgtaacgaag gaatcgatag aacgcataat ccagagttta ccatgataga ggcttatgcg gcttattggg attacaatga tgtaatgaaa tgtgtggaaa accttgttga gtatatcgta cgtgctttga ataacgggga aactcaggtt cagtattcac atttaaaatc aggacctcag gttgttgatt ttaaagctcc atggatccgt atgacgatga aagaaagtat ctctgtctat ggcggcgtcg atgtagactt acatgcagat catgaattac gtaaaatttt agaaacacaa acatctcttc cagagaaaac gtatgttcat gcctcacgag gagagctgat cgctttgcta tttgatgagc tggtttgtga taagctcatc gctccgcatc acattacaga tcatccatta gaaacaacgc cactttgtaa aacattgcgt tctggggatg aaactctggt agaacgattt gagagttttt gtttaggaaa agagttgtgt aatgcttatt cggaactcaa tgatccttta caacaacgaa aattgttgga agagcaaatg cgtaaaaagg ctttaaatcc tgacagcgaa taccatccta tagatgaaga atttctagaa gctctttgcc aaggaatgcc tcctgcagga ggatttggaa taggtatcga tcgattggtt atgatgttga cagacgccgc atccattcgg gatgtcctgt ttttccctgt tatgcggcgt atagaagcaa aaaaagatta a
Details for ChtrB.00612.a.B1.GE42404
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS VEVEYLQHED YLYRTSKLKE IRDLGINPYP YQYTDCLEVQ EIRNQFVDNE LGDSEAAFRK ETPKVRFAGR LVLFRSMGKN SFGQILDNDA KIQVMFNRDF SAVAGLAADA GISPIKFIEK KLDLGDILGL EGYLFFTHSG ELTVLVETVT LLCKSLISLP DKHAGLADKE IRYRKRWADL ISSEDVRKTF LTRSRILKLI REYMDQQSFL EVETPILQTI YGGAEATPFV TTLQALHAEM FLRISLEIAL KKLLVGGMSR VYEIGKVFRN EGIDRTHNPE FTMIEAYAAY WDYNDVMKCV ENLVEYIVRA LNNGETQVQY SHLKSGPQVV DFKAPWIRMT MKESISVYGG VDVDLHADHE LRKILETQTS LPEKTYVHAS RGELIALLFD ELVCDKLIAP HHITDHPLET TPLCKTLRSG DETLVERFES FCLGKELCNA YSELNDPLQQ RKLLEEQMRK KALNPDSEYH PIDEEFLEAL CQGMPPAGGF GIGIDRLVMM LTDAASIRDV LFFPVMRRIE AKKD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg tagaagttga atacttgcaa cacgaagatt atttgtatag aacaagcaag ctaaaggaaa tcagagattt gggcataaat ccttaccctt atcaatacac tgattgtctt gaagtacagg aaattcgtaa tcagtttgta gataacgaat taggagatag cgaagcggct tttcgtaaag agacacctaa ggtgcgtttt gccggacgac ttgttctttt ccgttctatg gggaaaaatt cttttgggca gatcctcgat aatgatgcaa agattcaagt gatgtttaat cgagattttt ctgcagtggc agggttagcc gcggatgctg ggatttctcc gattaaattt attgagaaga aacttgatct aggagacatc ttgggtctcg aagggtatct tttctttact cactcaggag aattaacggt tctcgttgaa acggtaacgt tgttatgtaa atccttaatt tctttgcctg ataagcatgc aggattagca gataaagaaa ttcgctatcg caaacgttgg gcagatctga tttcctcaga ggatgtgcgt aagactttct taacaagaag ccggattctt aagttgattc gtgagtacat ggatcagcag agctttttag aggtggaaac tcctatcctg caaaccatct acggaggagc agaagcaact ccttttgtta ccacgctgca agcgctacat gcagaaatgt tcctaagaat ttctctagag attgctttga aaaaactcct tgttggagga atgtcccgag tttatgaaat cggcaaagtt ttccgtaacg aaggaatcga tagaacgcat aatccagagt ttaccatgat agaggcttat gcggcttatt gggattacaa tgatgtaatg aaatgtgtgg aaaaccttgt tgagtatatc gtacgtgctt tgaataacgg ggaaactcag gttcagtatt cacatttaaa atcaggacct caggttgttg attttaaagc tccatggatc cgtatgacga tgaaagaaag tatctctgtc tatggcggcg tcgatgtaga cttacatgca gatcatgaat tacgtaaaat tttagaaaca caaacatctc ttccagagaa aacgtatgtt catgcctcac gaggagagct gatcgctttg ctatttgatg agctggtttg tgataagctc atcgctccgc atcacattac agatcatcca ttagaaacaa cgccactttg taaaacattg cgttctgggg atgaaactct ggtagaacga tttgagagtt tttgtttagg aaaagagttg tgtaatgctt attcggaact caatgatcct ttacaacaac gaaaattgtt ggaagagcaa atgcgtaaaa aggctttaaa tcctgacagc gaataccatc ctatagatga agaatttcta gaagctcttt gccaaggaat gcctcctgca ggaggatttg gaataggtat cgatcgattg gttatgatgt tgacagacgc cgcatccatt cgggatgtcc tgtttttccc tgttatgcgg cgtatagaag caaaaaaaga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat